Restriction map for
pF1KB5012
(Circular) (Six-base) MAPSORT of: pF1KB5012.seq Check: 9481 from: 1 to: 4742
pF1KB5012 4742 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 302 911 1780 302
Size: 609 869 3264
Fragments arranged by size:
3264 869 609
Acc65I G'GTAC_C
Cuts at: 1758 1758
Size: 4742
AclI AA'CG_TT
Cuts at: 4582 4582
Size: 4742
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 1165 2567 2999 3352 4014 148
Size: 1017 1402 432 353 662 876
Fragments arranged by size:
1402 1017 876 662 432 353
AfeI AGC'GCT
Cuts at: 1516 1516
Size: 4742
AflIII A'CryG_T
Cuts at: 2094 3894 4175 2094
Size: 1800 281 2661
Fragments arranged by size:
2661 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 783 1707 2047 3485 783
Size: 924 340 1438 2040
Fragments arranged by size:
2040 1438 924 340
ApaLI G'TGCA_C
Cuts at: 3580 3580
Size: 4742
ApoI r'AATT_y
Cuts at: 475 1746 4095 4351 475
Size: 1271 2349 256 866
Fragments arranged by size:
2349 1271 866 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4742
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4742
AvaI C'yCGr_G
Cuts at: 1762 1762
Size: 4742
BamHI G'GATC_C
Cuts at: 207 1767 207
Size: 1560 3182
BanI G'GyrC_C
Cuts at: 1758 2172 2420 2455 1758
Size: 414 248 35 4045
Fragments arranged by size:
4045 414 248 35
BanII G_rGCy'C
Cuts at: 1756 2786 1756
Size: 1030 3712
BbeI G_GCGC'C
Cuts at: 2176 2424 2176
Size: 248 4494
BbsI GAAGACnn'nnnn_
Cuts at: 995 995
Size: 4742
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 282 316 1295 1329 282
Size: 34 979 34 3695
Fragments arranged by size:
3695 979 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1378 2640 3696 4742 1378
Size: 1262 1056 1046 1378
Fragments arranged by size:
1378 1262 1056 1046
BclI T'GATC_A
Cuts at: 2262 2262
Size: 4742
BglI GCCn_nnn'nGGC
Cuts at: 463 463
Size: 4742
BglII A'GATC_T
Cuts at: 756 2257 4294 756
Size: 1501 2037 1204
Fragments arranged by size:
2037 1501 1204
BlpI GC'TnA_GC
Cuts at: 1854 1854
Size: 4742
Bme1580I G_kGCm'C
Cuts at: 610 2367 2460 3584 610
Size: 1757 93 1124 1768
Fragments arranged by size:
1768 1757 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 327 1954 2035 2370 4514 327
Size: 1627 81 335 2144 555
Fragments arranged by size:
2144 1627 555 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3109 3109
Size: 4742
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1351 1396 1467 1351
Size: 45 71 4626
Fragments arranged by size:
4626 71 45
Bpu10I CC'TnA_GC
Cuts at: 151 151
Size: 4742
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1099 1333 1918 2797 3511 3809 1099
Size: 234 585 879 714 298 2032
Fragments arranged by size:
2032 879 714 585 298 234
BsaI GGTCTCn'nnnn_
Cuts at: 724 4446 724
Size: 3722 1020
BsaAI yAC'GTr
Cuts at: 222 2095 222
Size: 1873 2869
BsaBI GATnn'nnATC
Cuts at: 6 2256 4293 6
Size: 2250 2037 455
Fragments arranged by size:
2250 2037 455
BsaHI Gr'CG_yC
Cuts at: 2173 2421 3123 4619 2173
Size: 248 702 1496 2296
Fragments arranged by size:
2296 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1931 2452 3130 3541 3688 3962 1931
Size: 521 678 411 147 274 2711
Fragments arranged by size:
2711 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 445 475 1465 1495 445
Size: 30 990 30 3692
Fragments arranged by size:
3692 990 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 409 697 1207 409
Size: 288 510 3944
Fragments arranged by size:
3944 510 288
BseYI C'CCAG_C
Cuts at: 798 1611 2167 2925 3590 3998 798
Size: 813 556 758 665 408 1542
Fragments arranged by size:
1542 813 758 665 556 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1612 1612
Size: 4742
BsiEI CG_ry'CG
Cuts at: 104 2284 2330 3560 104
Size: 2180 46 1230 1286
Fragments arranged by size:
2180 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1097 1115 1756 2534 2724 3584 1097
Size: 18 641 778 190 860 2255
Fragments arranged by size:
2255 860 778 641 190 18
Bsp1286I G_dGCh'C
Cuts at: 610 1097 1115 1756 2367 2460 2534 2724 2786
Size: 487 18 641 611 93 74 190 62
Cuts at: 2786 3584 610
Size: 798 1768
Fragments arranged by size:
1768 798 641 611 487 190 93 74
62 18
BspHI T'CATG_A
Cuts at: 479 632 1601 3174 479
Size: 153 969 1573 2047
Fragments arranged by size:
2047 1573 969 153
BspMI ACCTGCnnnn'nnnn_
Cuts at: 105 1792 2006 2308 2689 105
Size: 1687 214 302 381 2158
Fragments arranged by size:
2158 1687 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1158 3034 3088 4572 4737 50
Size: 1108 1876 54 1484 165 55
Fragments arranged by size:
1876 1484 1108 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2654 2654
Size: 4742
BsrFI r'CCGG_y
Cuts at: 1538 2740 2921 1538
Size: 1202 181 3359
Fragments arranged by size:
3359 1202 181
BsrGI T'GTAC_A
Cuts at: 227 227
Size: 4742
BssSI C'ACGA_G
Cuts at: 409 3013 3721 409
Size: 2604 708 1430
Fragments arranged by size:
2604 1430 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2119 2119
Size: 4742
BstBI TT'CG_AA
Cuts at: 3103 3103
Size: 4742
BstXI CCAn_nnnn'nTGG
Cuts at: 867 969 1329 1449 867
Size: 102 360 120 4160
Fragments arranged by size:
4160 360 120 102
BstYI r'GATC_y
Cuts at: 1 207 621 756 1697 1767 2234 2257 2592
Size: 206 414 135 941 70 467 23 335
Cuts at: 2592 2838 3144 3156 3242 4294 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 941 467 449 414 335 306 246
206 135 86 70 23 12
BstZ17I GTA'TAC
Cuts at: 912 912
Size: 4742
Bsu36I CC'TnA_GG
Cuts at: 1586 1586
Size: 4742
BtgI C'CryG_G
Cuts at: 107 1448 1574 107
Size: 1341 126 3275
Fragments arranged by size:
3275 1341 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1441 2692 2873 3036 1441
Size: 1251 181 163 3147
Fragments arranged by size:
3147 1251 181 163
BtsI GCAGTG_nn'
Cuts at: 1985 1985
Size: 4742
ClaI AT'CG_AT
Cuts at: 167 167
Size: 4742
DraI TTT'AAA
Cuts at: 1741 1741
Size: 4742
DrdI GACnn_nn'nnGTC
Cuts at: 2448 3792 2448
Size: 1344 3398
EaeI y'GGCC_r
Cuts at: 1577 2327 2501 2892 2919 4264 1577
Size: 750 174 391 27 1345 2055
Fragments arranged by size:
2055 1345 750 391 174 27
EagI C'GGCC_G
Cuts at: 2327 2327
Size: 4742
EarI CTCTTCn'nnn_
Cuts at: 683 2765 2975 683
Size: 2082 210 2450
Fragments arranged by size:
2450 2082 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2950 3692 3838 4552 2950
Size: 742 146 714 3140
Fragments arranged by size:
3140 742 714 146
EcoICRI GAG'CTC
Cuts at: 1754 1754
Size: 4742
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 1165 1351 1396 1467 2567 2999 3352 4014
Size: 1017 186 45 71 1100 432 353 662
Cuts at: 4014 148
Size: 876
Fragments arranged by size:
1100 1017 876 662 432 353 186 71
45
EcoO109I rG'GnC_Cy
Cuts at: 147 1647 1881 147
Size: 1500 234 3008
Fragments arranged by size:
3008 1500 234
EcoRI G'AATT_C
Cuts at: 1746 1746
Size: 4742
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 775 843 2761 775
Size: 68 1918 2756
Fragments arranged by size:
2756 1918 68
FspI TGC'GCA
Cuts at: 2523 2523
Size: 4742
HaeII r_GCGC'y
Cuts at: 1471 1518 2176 2424 3654 4425 1471
Size: 47 658 248 1230 771 1788
Fragments arranged by size:
1788 1230 771 658 248 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1465 1497 2584 2614 2616 2646 1465
Size: 32 1087 30 2 30 3561
Fragments arranged by size:
3561 1087 32 30 30 2
HincII GTy'rAC
Cuts at: 303 1781 303
Size: 1478 3264
KasI G'GCGC_C
Cuts at: 2172 2420 2172
Size: 248 4494
KpnI G_GTAC'C
Cuts at: 1762 1762
Size: 4742
MluI A'CGCG_T
Cuts at: 4175 4175
Size: 4742
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 497 3501 3685 497
Size: 3004 184 1554
Fragments arranged by size:
3004 1554 184
MscI TGG'CCA
Cuts at: 2503 2503
Size: 4742
MslI CAynn'nnrTG
Cuts at: 880 967 1453 2731 2858 880
Size: 87 486 1278 127 2764
Fragments arranged by size:
2764 1278 486 127 87
MspA1I CmG'CkG
Cuts at: 907 1341 1374 1414 1487 1853 2167 2527 3311
Size: 434 33 40 73 366 314 360 784
Cuts at: 3311 3556 907
Size: 245 2093
Fragments arranged by size:
2093 784 434 366 360 314 245 73
40 33
NaeI GCC'GGC
Cuts at: 2923 2923
Size: 4742
NarI GG'CG_CC
Cuts at: 2173 2421 2173
Size: 248 4494
NcoI C'CATG_G
Cuts at: 107 1448 1574 107
Size: 1341 126 3275
Fragments arranged by size:
3275 1341 126
NdeI CA'TA_TG
Cuts at: 1014 1014
Size: 4742
NgoMIV G'CCGG_C
Cuts at: 2921 2921
Size: 4742
NspI r_CATG'y
Cuts at: 498 1795 2295 3898 498
Size: 1297 500 1603 1342
Fragments arranged by size:
1603 1342 1297 500
PciI A'CATG_T
Cuts at: 3894 3894
Size: 4742
PfoI T'CCnGG_A
Cuts at: 1328 1628 1328
Size: 300 4442
PmeI GTTT'AAAC
Cuts at: 1741 1741
Size: 4742
PmlI CAC'GTG
Cuts at: 222 222
Size: 4742
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 4710
PpuMI rG'GwC_Cy
Cuts at: 1647 1647
Size: 4742
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 796 828 796
Size: 32 4710
PstI C_TGCA'G
Cuts at: 1789 2474 1789
Size: 685 4057
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4742
PvuII CAG'CTG
Cuts at: 1341 1374 1487 2167 2527 1341
Size: 33 113 680 360 3556
Fragments arranged by size:
3556 680 360 113 33
RsrII CG'GwC_CG
Cuts at: 2937 2937
Size: 4742
SacI G_AGCT'C
Cuts at: 1756 1756
Size: 4742
SalI G'TCGA_C
Cuts at: 301 1779 301
Size: 1478 3264
SapI GCTCTTCn'nnn_
Cuts at: 2765 2975 2765
Size: 210 4532
SbfI CC_TGCA'GG
Cuts at: 1789 1789
Size: 4742
ScaI AGT'ACT
Cuts at: 4259 4259
Size: 4742
SfcI C'TryA_G
Cuts at: 33 1785 2470 3438 3629 33
Size: 1752 685 968 191 1146
Fragments arranged by size:
1752 1146 968 685 191
SfoI GGC'GCC
Cuts at: 2174 2422 2174
Size: 248 4494
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4742
SmaI CCC'GGG
Cuts at: 1764 1764
Size: 4742
SmlI C'TyrA_G
Cuts at: 1114 1348 1897 2812 3526 3788 1114
Size: 234 549 915 714 262 2068
Fragments arranged by size:
2068 915 714 549 262 234
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4742
SphI G_CATG'C
Cuts at: 1795 2295 1795
Size: 500 4242
StyI C'CwwG_G
Cuts at: 107 654 860 1448 1574 1876 2230 107
Size: 547 206 588 126 302 354 2619
Fragments arranged by size:
2619 588 547 354 302 206 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2325 2458 2917 3126 2325
Size: 133 459 209 3941
Fragments arranged by size:
3941 459 209 133
TatI w'GTAC_w
Cuts at: 227 1052 1532 4257 227
Size: 825 480 2725 712
Fragments arranged by size:
2725 825 712 480
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1682 1714 4259 4291 1682
Size: 32 2545 32 2133
Fragments arranged by size:
2545 2133 32 32
Tth111I GACn'n_nGTC
Cuts at: 2539 2539
Size: 4742
XbaI T'CTAG_A
Cuts at: 351 1773 351
Size: 1422 3320
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2237 2237
Size: 4742
XmaI C'CCGG_G
Cuts at: 1762 1762
Size: 4742
XmnI GAAnn'nnTTC
Cuts at: 487 758 487
Size: 271 4471
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PfoI
PmeI PmlI PpiI PpuMI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI
BsiWI BsmI BsmBI BspEI BssHII BstEII CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PasI PflMI PshAI PsiI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI