Restriction map for
pF1KB5017
(Circular) (Six-base) MAPSORT of: pF1KB5017.seq Check: 4878 from: 1 to: 4127
pF1KB5017 4127 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1165 1165
Size: 4127
Acc65I G'GTAC_C
Cuts at: 1143 1143
Size: 4127
AclI AA'CG_TT
Cuts at: 3967 3967
Size: 4127
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 665 1080 1952 2384 2737 3399 665
Size: 415 872 432 353 662 1393
Fragments arranged by size:
1393 872 662 432 415 353
AflIII A'CryG_T
Cuts at: 968 1479 3279 3560 968
Size: 511 1800 281 1535
Fragments arranged by size:
1800 1535 511 281
AleI CACnn'nnGTG
Cuts at: 121 121
Size: 4127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 222 925 222
Size: 703 3424
AlwNI CAG_nnn'CTG
Cuts at: 879 1432 2870 879
Size: 553 1438 2136
Fragments arranged by size:
2136 1438 553
ApaI G_GGCC'C
Cuts at: 620 672 620
Size: 52 4075
ApaLI G'TGCA_C
Cuts at: 2965 2965
Size: 4127
ApoI r'AATT_y
Cuts at: 1131 3480 3736 1131
Size: 2349 256 1522
Fragments arranged by size:
2349 1522 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4127
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4127
AvaI C'yCGr_G
Cuts at: 421 671 913 1147 421
Size: 250 242 234 3401
Fragments arranged by size:
3401 250 242 234
BamHI G'GATC_C
Cuts at: 1152 1152
Size: 4127
BanI G'GyrC_C
Cuts at: 232 487 844 1143 1557 1805 1840 232
Size: 255 357 299 414 248 35 2519
Fragments arranged by size:
2519 414 357 299 255 248 35
BanII G_rGCy'C
Cuts at: 349 539 620 672 826 1141 2171 349
Size: 190 81 52 154 315 1030 2305
Fragments arranged by size:
2305 1030 315 190 154 81 52
BbeI G_GCGC'C
Cuts at: 1561 1809 1561
Size: 248 3879
BbvCI CC'TCA_GC
Cuts at: 310 310
Size: 4127
BciVI GTATCCnnnnn_n'
Cuts at: 2025 3081 4127 2025
Size: 1056 1046 2025
Fragments arranged by size:
2025 1056 1046
BclI T'GATC_A
Cuts at: 1647 1647
Size: 4127
BglI GCCn_nnn'nGGC
Cuts at: 1049 1049
Size: 4127
BglII A'GATC_T
Cuts at: 1642 3679 1642
Size: 2037 2090
BlpI GC'TnA_GC
Cuts at: 1239 1239
Size: 4127
Bme1580I G_kGCm'C
Cuts at: 235 620 660 672 1752 1845 2969 235
Size: 385 40 12 1080 93 1124 1393
Fragments arranged by size:
1393 1124 1080 385 93 40 12
BmgBI CAC'GTC
Cuts at: 779 779
Size: 4127
BmrI ACTGGGnnnn_n'
Cuts at: 188 725 1339 1420 1755 3899 188
Size: 537 614 81 335 2144 416
Fragments arranged by size:
2144 614 537 416 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2494 2494
Size: 4127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 426 685 852 1017 426
Size: 259 167 165 3536
Fragments arranged by size:
3536 259 167 165
Bpu10I CC'TnA_GC
Cuts at: 310 1024 310
Size: 714 3413
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 304 1062 1303 2182 2896 3194 165
Size: 139 758 241 879 714 298 1098
Fragments arranged by size:
1098 879 758 714 298 241 139
BsaI GGTCTCn'nnnn_
Cuts at: 362 3831 362
Size: 3469 658
BsaAI yAC'GTr
Cuts at: 1480 1480
Size: 4127
BsaBI GATnn'nnATC
Cuts at: 6 1641 3678 6
Size: 1635 2037 455
Fragments arranged by size:
2037 1635 455
BsaHI Gr'CG_yC
Cuts at: 1083 1558 1806 2508 4004 1083
Size: 475 248 702 1496 1206
Fragments arranged by size:
1496 1206 702 475 248
BsaWI w'CCGG_w
Cuts at: 1316 1837 2515 2926 3073 3347 1316
Size: 521 678 411 147 274 2096
Fragments arranged by size:
2096 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 298 298
Size: 4127
BseYI C'CCAG_C
Cuts at: 280 721 985 1552 2310 2975 3383 280
Size: 441 264 567 758 665 408 1024
Fragments arranged by size:
1024 758 665 567 441 408 264
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 218
Size: 4127
BsiEI CG_ry'CG
Cuts at: 104 1669 1715 2945 104
Size: 1565 46 1230 1286
Fragments arranged by size:
1565 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 192 349 539 1141 1919 2109 2969 192
Size: 157 190 602 778 190 860 1350
Fragments arranged by size:
1350 860 778 602 190 190 157
Bsp1286I G_dGCh'C
Cuts at: 192 235 349 539 620 660 672 826 1141
Size: 43 114 190 81 40 12 154 315
Cuts at: 1141 1752 1845 1919 2109 2171 2969 192
Size: 611 93 74 190 62 798 1350
Fragments arranged by size:
1350 798 611 315 190 190 154 114
93 81 74 62 43 40 12
BspHI T'CATG_A
Cuts at: 2559 2559
Size: 4127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1177 1391 1693 2074 1177
Size: 214 302 381 3230
Fragments arranged by size:
3230 381 302 214
BsrBI CCG'CTC
Cuts at: 50 340 374 396 2419 2473 3957 4122 50
Size: 290 34 22 2023 54 1484 165 55
Fragments arranged by size:
2023 1484 290 165 55 54 34 22
BsrDI GCAATG_nn'
Cuts at: 2039 2039
Size: 4127
BsrFI r'CCGG_y
Cuts at: 2125 2306 2125
Size: 181 3946
BssSI C'ACGA_G
Cuts at: 121 532 2398 3106 121
Size: 411 1866 708 1142
Fragments arranged by size:
1866 1142 708 411
BstAPI GCAn_nnn'nTGC
Cuts at: 1504 1504
Size: 4127
BstBI TT'CG_AA
Cuts at: 2488 2488
Size: 4127
BstYI r'GATC_y
Cuts at: 1 1152 1619 1642 1977 2223 2529 2541 2627
Size: 1151 467 23 335 246 306 12 86
Cuts at: 2627 3679 1
Size: 1052 449
Fragments arranged by size:
1151 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 992 992
Size: 4127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2077 2258 2421 2077
Size: 181 163 3783
Fragments arranged by size:
3783 181 163
BtsI GCAGTG_nn'
Cuts at: 233 1370 233
Size: 1137 2990
DraI TTT'AAA
Cuts at: 1126 1126
Size: 4127
DrdI GACnn_nn'nnGTC
Cuts at: 1833 3177 1833
Size: 1344 2783
EaeI y'GGCC_r
Cuts at: 429 662 1712 1886 2277 2304 3649 429
Size: 233 1050 174 391 27 1345 907
Fragments arranged by size:
1345 1050 907 391 233 174 27
EagI C'GGCC_G
Cuts at: 1712 1712
Size: 4127
EarI CTCTTCn'nnn_
Cuts at: 641 946 2150 2360 641
Size: 305 1204 210 2408
Fragments arranged by size:
2408 1204 305 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2335 3077 3223 3937 2335
Size: 742 146 714 2525
Fragments arranged by size:
2525 742 714 146
EcoICRI GAG'CTC
Cuts at: 347 537 1139 347
Size: 190 602 3335
Fragments arranged by size:
3335 602 190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 426 665 685 852 1017 1080 1952 2384 2737
Size: 239 20 167 165 63 872 432 353
Cuts at: 2737 3399 426
Size: 662 1154
Fragments arranged by size:
1154 872 662 432 353 239 167 165
63 20
EcoO109I rG'GnC_Cy
Cuts at: 617 668 675 1266 617
Size: 51 7 591 3478
Fragments arranged by size:
3478 591 51 7
EcoRI G'AATT_C
Cuts at: 1131 1131
Size: 4127
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2146 2146
Size: 4127
FspI TGC'GCA
Cuts at: 1908 1908
Size: 4127
HaeII r_GCGC'y
Cuts at: 654 809 1561 1809 3039 3810 654
Size: 155 752 248 1230 771 971
Fragments arranged by size:
1230 971 771 752 248 155
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 988 1020 1094 1126 1969 1999 2001 2031 988
Size: 32 74 32 843 30 2 30 3084
Fragments arranged by size:
3084 843 74 32 32 30 30 2
HincII GTy'rAC
Cuts at: 438 1166 438
Size: 728 3399
KasI G'GCGC_C
Cuts at: 1557 1805 1557
Size: 248 3879
KpnI G_GTAC'C
Cuts at: 1147 1147
Size: 4127
MluI A'CGCG_T
Cuts at: 3560 3560
Size: 4127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2886 3070 2886
Size: 184 3943
MscI TGG'CCA
Cuts at: 1888 1888
Size: 4127
MslI CAynn'nnrTG
Cuts at: 121 2116 2243 121
Size: 1995 127 2005
Fragments arranged by size:
2005 1995 127
MspA1I CmG'CkG
Cuts at: 623 685 739 751 1238 1552 1912 2696 2941
Size: 62 54 12 487 314 360 784 245
Cuts at: 2941 623
Size: 1809
Fragments arranged by size:
1809 784 487 360 314 245 62 54
12
NaeI GCC'GGC
Cuts at: 2308 2308
Size: 4127
NarI GG'CG_CC
Cuts at: 1558 1806 1558
Size: 248 3879
NgoMIV G'CCGG_C
Cuts at: 2306 2306
Size: 4127
NruI TCG'CGA
Cuts at: 351 351
Size: 4127
NspI r_CATG'y
Cuts at: 972 1180 1680 3283 972
Size: 208 500 1603 1816
Fragments arranged by size:
1816 1603 500 208
PciI A'CATG_T
Cuts at: 968 3279 968
Size: 2311 1816
PflMI CCAn_nnn'nTGG
Cuts at: 879 879
Size: 4127
PmeI GTTT'AAAC
Cuts at: 1126 1126
Size: 4127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2573 2605 2573
Size: 32 4095
PpuMI rG'GwC_Cy
Cuts at: 675 675
Size: 4127
PspOMI G'GGCC_C
Cuts at: 616 668 616
Size: 52 4075
PstI C_TGCA'G
Cuts at: 1174 1859 1174
Size: 685 3442
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4127
PvuII CAG'CTG
Cuts at: 1552 1912 1552
Size: 360 3767
RsrII CG'GwC_CG
Cuts at: 2322 2322
Size: 4127
SacI G_AGCT'C
Cuts at: 349 539 1141 349
Size: 190 602 3335
Fragments arranged by size:
3335 602 190
SalI G'TCGA_C
Cuts at: 1164 1164
Size: 4127
SapI GCTCTTCn'nnn_
Cuts at: 641 2150 2360 641
Size: 1509 210 2408
Fragments arranged by size:
2408 1509 210
SbfI CC_TGCA'GG
Cuts at: 1174 1174
Size: 4127
ScaI AGT'ACT
Cuts at: 3644 3644
Size: 4127
SfcI C'TryA_G
Cuts at: 33 183 1170 1855 2823 3014 33
Size: 150 987 685 968 191 1146
Fragments arranged by size:
1146 987 968 685 191 150
SfoI GGC'GCC
Cuts at: 1559 1807 1559
Size: 248 3879
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4127
SmaI CCC'GGG
Cuts at: 423 673 915 1149 423
Size: 250 242 234 3401
Fragments arranged by size:
3401 250 242 234
SmlI C'TyrA_G
Cuts at: 144 319 1077 1282 2197 2911 3173 144
Size: 175 758 205 915 714 262 1098
Fragments arranged by size:
1098 915 758 714 262 205 175
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4127
SphI G_CATG'C
Cuts at: 1180 1680 1180
Size: 500 3627
StyI C'CwwG_G
Cuts at: 1067 1261 1615 1067
Size: 194 354 3579
Fragments arranged by size:
3579 354 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 424 1710 1843 2302 2511 424
Size: 1286 133 459 209 2040
Fragments arranged by size:
2040 1286 459 209 133
TatI w'GTAC_w
Cuts at: 563 3642 563
Size: 3079 1048
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 512 544 3644 3676 512
Size: 32 3100 32 963
Fragments arranged by size:
3100 963 32 32
Tth111I GACn'n_nGTC
Cuts at: 1924 1924
Size: 4127
XbaI T'CTAG_A
Cuts at: 1158 1158
Size: 4127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 762 876 1622 762
Size: 114 746 3267
Fragments arranged by size:
3267 746 114
XmaI C'CCGG_G
Cuts at: 421 671 913 1147 421
Size: 250 242 234 3401
Fragments arranged by size:
3401 250 242 234
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NruI
NspI PciI PflMI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BmtI BsaXI BsiWI
BsmI BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI