Restriction map for
pF1KB5019
(Circular) (Six-base) MAPSORT of: pF1KB5019.seq Check: 5220 from: 1 to: 4628
pF1KB5019 4628 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 433 433
Size: 4628
AccI GT'mk_AC
Cuts at: 1666 1666
Size: 4628
Acc65I G'GTAC_C
Cuts at: 1644 1644
Size: 4628
AclI AA'CG_TT
Cuts at: 4468 4468
Size: 4628
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 497 1210 1222 2453 2885 3238 3900 436
Size: 61 713 12 1231 432 353 662 1164
Fragments arranged by size:
1231 1164 713 662 432 353 61 12
AfeI AGC'GCT
Cuts at: 128 128
Size: 4628
AflIII A'CryG_T
Cuts at: 1980 3780 4061 1980
Size: 1800 281 2547
Fragments arranged by size:
2547 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1933 3371 1933
Size: 1438 3190
ApaLI G'TGCA_C
Cuts at: 3466 3466
Size: 4628
ApoI r'AATT_y
Cuts at: 538 547 973 1260 1306 1632 3981 4237 538
Size: 9 426 287 46 326 2349 256 929
Fragments arranged by size:
2349 929 426 326 287 256 46 9
AseI AT'TA_AT
Cuts at: 20 20
Size: 4628
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
AvaI C'yCGr_G
Cuts at: 135 1648 135
Size: 1513 3115
AvrII C'CTAG_G
Cuts at: 460 460
Size: 4628
BamHI G'GATC_C
Cuts at: 1653 1653
Size: 4628
BanI G'GyrC_C
Cuts at: 1644 2058 2306 2341 1644
Size: 414 248 35 3931
Fragments arranged by size:
3931 414 248 35
BanII G_rGCy'C
Cuts at: 238 1349 1642 2672 238
Size: 1111 293 1030 2194
Fragments arranged by size:
2194 1111 1030 293
BbeI G_GCGC'C
Cuts at: 2062 2310 2062
Size: 248 4380
BbsI GAAGACnn'nnnn_
Cuts at: 860 860
Size: 4628
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4594
BciVI GTATCCnnnnn_n'
Cuts at: 2526 3582 4628 2526
Size: 1056 1046 2526
Fragments arranged by size:
2526 1056 1046
BclI T'GATC_A
Cuts at: 2148 2148
Size: 4628
BglII A'GATC_T
Cuts at: 2143 4180 2143
Size: 2037 2591
BlpI GC'TnA_GC
Cuts at: 1375 1740 1375
Size: 365 4263
Bme1580I G_kGCm'C
Cuts at: 992 2253 2346 3470 992
Size: 1261 93 1124 2150
Fragments arranged by size:
2150 1261 1124 93
BmgBI CAC'GTC
Cuts at: 186 186
Size: 4628
BmrI ACTGGGnnnn_n'
Cuts at: 1840 1921 2256 4400 1840
Size: 81 335 2144 2068
Fragments arranged by size:
2144 2068 335 81
BmtI G_CTAG'C
Cuts at: 1016 1016
Size: 4628
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 627 1149 2995 627
Size: 522 1846 2260
Fragments arranged by size:
2260 1846 522
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 963 1088 1174 1363 1460 963
Size: 125 86 189 97 4131
Fragments arranged by size:
4131 189 125 97 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 660 1804 2683 3397 3695 660
Size: 1144 879 714 298 1593
Fragments arranged by size:
1593 1144 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4332 4332
Size: 4628
BsaAI yAC'GTr
Cuts at: 1981 1981
Size: 4628
BsaBI GATnn'nnATC
Cuts at: 6 1184 2142 4179 6
Size: 1178 958 2037 455
Fragments arranged by size:
2037 1178 958 455
BsaHI Gr'CG_yC
Cuts at: 2059 2307 3009 4505 2059
Size: 248 702 1496 2182
Fragments arranged by size:
2182 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1817 2338 3016 3427 3574 3848 1817
Size: 521 678 411 147 274 2597
Fragments arranged by size:
2597 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1336 1366 1336
Size: 30 4598
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1141 1141
Size: 4628
BseYI C'CCAG_C
Cuts at: 985 2053 2811 3476 3884 985
Size: 1068 758 665 408 1729
Fragments arranged by size:
1729 1068 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2170 2216 3446 104
Size: 2066 46 1230 1286
Fragments arranged by size:
2066 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1063 1642 2420 2610 3470 1063
Size: 579 778 190 860 2221
Fragments arranged by size:
2221 860 778 579 190
Bsp1286I G_dGCh'C
Cuts at: 238 992 1063 1349 1642 2253 2346 2420 2610
Size: 754 71 286 293 611 93 74 190
Cuts at: 2610 2672 3470 238
Size: 62 798 1396
Fragments arranged by size:
1396 798 754 611 293 286 190 93
74 71 62
BspHI T'CATG_A
Cuts at: 3060 3060
Size: 4628
BspMI ACCTGCnnnn'nnnn_
Cuts at: 433 1678 1892 2194 2575 433
Size: 1245 214 302 381 2486
Fragments arranged by size:
2486 1245 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2920 2974 4458 4623 50
Size: 2870 54 1484 165 55
Fragments arranged by size:
2870 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1233 2540 1233
Size: 1307 3321
BsrFI r'CCGG_y
Cuts at: 2626 2807 2626
Size: 181 4447
BssSI C'ACGA_G
Cuts at: 2899 3607 2899
Size: 708 3920
BstAPI GCAn_nnn'nTGC
Cuts at: 2005 2005
Size: 4628
BstBI TT'CG_AA
Cuts at: 2989 2989
Size: 4628
BstYI r'GATC_y
Cuts at: 1 1617 1653 2120 2143 2478 2724 3030 3042
Size: 1616 36 467 23 335 246 306 12
Cuts at: 3042 3128 4180 1
Size: 86 1052 449
Fragments arranged by size:
1616 1052 467 449 335 306 246 86
36 23 12
BtgI C'CryG_G
Cuts at: 158 158
Size: 4628
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2578 2759 2922 2578
Size: 181 163 4284
Fragments arranged by size:
4284 181 163
BtsI GCAGTG_nn'
Cuts at: 282 362 1061 1871 282
Size: 80 699 810 3039
Fragments arranged by size:
3039 810 699 80
DraI TTT'AAA
Cuts at: 1627 1627
Size: 4628
DrdI GACnn_nn'nnGTC
Cuts at: 2334 3678 2334
Size: 1344 3284
EaeI y'GGCC_r
Cuts at: 1515 2213 2387 2778 2805 4150 1515
Size: 698 174 391 27 1345 1993
Fragments arranged by size:
1993 1345 698 391 174 27
EagI C'GGCC_G
Cuts at: 2213 2213
Size: 4628
EarI CTCTTCn'nnn_
Cuts at: 1186 2651 2861 1186
Size: 1465 210 2953
Fragments arranged by size:
2953 1465 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 106 2836 3578 3724 4438 106
Size: 2730 742 146 714 296
Fragments arranged by size:
2730 742 714 296 146
EcoICRI GAG'CTC
Cuts at: 1640 1640
Size: 4628
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 497 963 1088 1174 1210 1222 1363 1460
Size: 61 466 125 86 36 12 141 97
Cuts at: 1460 2453 2885 3238 3900 436
Size: 993 432 353 662 1164
Fragments arranged by size:
1164 993 662 466 432 353 141 125
97 86 61 36 12
EcoO109I rG'GnC_Cy
Cuts at: 1767 1767
Size: 4628
EcoRI G'AATT_C
Cuts at: 1632 1632
Size: 4628
EcoRV GAT'ATC
Cuts at: 406 406
Size: 4628
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2647 2647
Size: 4628
FspI TGC'GCA
Cuts at: 2409 2409
Size: 4628
HaeII r_GCGC'y
Cuts at: 130 148 2062 2310 3540 4311 130
Size: 18 1914 248 1230 771 447
Fragments arranged by size:
1914 1230 771 447 248 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 220 252 698 730 2470 2500 2502 2532 220
Size: 32 446 32 1740 30 2 30 2316
Fragments arranged by size:
2316 1740 446 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1667 1667
Size: 4628
KasI G'GCGC_C
Cuts at: 2058 2306 2058
Size: 248 4380
KpnI G_GTAC'C
Cuts at: 1648 1648
Size: 4628
MluI A'CGCG_T
Cuts at: 4061 4061
Size: 4628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 206 251 433 959 3387 3571 206
Size: 45 182 526 2428 184 1263
Fragments arranged by size:
2428 1263 526 184 182 45
MscI TGG'CCA
Cuts at: 2389 2389
Size: 4628
MslI CAynn'nnrTG
Cuts at: 668 2617 2744 668
Size: 1949 127 2552
Fragments arranged by size:
2552 1949 127
MspA1I CmG'CkG
Cuts at: 160 175 1739 2053 2413 3197 3442 160
Size: 15 1564 314 360 784 245 1346
Fragments arranged by size:
1564 1346 784 360 314 245 15
NaeI GCC'GGC
Cuts at: 2809 2809
Size: 4628
NarI GG'CG_CC
Cuts at: 2059 2307 2059
Size: 248 4380
NdeI CA'TA_TG
Cuts at: 1440 1440
Size: 4628
NgoMIV G'CCGG_C
Cuts at: 2807 2807
Size: 4628
NheI G'CTAG_C
Cuts at: 1012 1012
Size: 4628
NspI r_CATG'y
Cuts at: 1681 2181 3784 1681
Size: 500 1603 2525
Fragments arranged by size:
2525 1603 500
PciI A'CATG_T
Cuts at: 3780 3780
Size: 4628
PflMI CCAn_nnn'nTGG
Cuts at: 941 941
Size: 4628
PfoI T'CCnGG_A
Cuts at: 815 977 815
Size: 162 4466
PmeI GTTT'AAAC
Cuts at: 1627 1627
Size: 4628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3074 3106 3074
Size: 32 4596
PstI C_TGCA'G
Cuts at: 318 1169 1675 2360 318
Size: 851 506 685 2586
Fragments arranged by size:
2586 851 685 506
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4628
PvuII CAG'CTG
Cuts at: 2053 2413 2053
Size: 360 4268
RsrII CG'GwC_CG
Cuts at: 2823 2823
Size: 4628
SacI G_AGCT'C
Cuts at: 1642 1642
Size: 4628
SacII CC_GC'GG
Cuts at: 161 161
Size: 4628
SalI G'TCGA_C
Cuts at: 1665 1665
Size: 4628
SapI GCTCTTCn'nnn_
Cuts at: 2651 2861 2651
Size: 210 4418
SbfI CC_TGCA'GG
Cuts at: 1169 1675 1169
Size: 506 4122
ScaI AGT'ACT
Cuts at: 4145 4145
Size: 4628
SfcI C'TryA_G
Cuts at: 33 314 572 1007 1165 1671 2356 3324 3515
Size: 281 258 435 158 506 685 968 191
Cuts at: 3515 33
Size: 1146
Fragments arranged by size:
1146 968 685 506 435 281 258 191
158
SfoI GGC'GCC
Cuts at: 2060 2308 2060
Size: 248 4380
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
SmaI CCC'GGG
Cuts at: 137 1650 137
Size: 1513 3115
SmlI C'TyrA_G
Cuts at: 639 1783 2698 3412 3674 639
Size: 1144 915 714 262 1593
Fragments arranged by size:
1593 1144 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4628
SphI G_CATG'C
Cuts at: 1681 2181 1681
Size: 500 4128
StyI C'CwwG_G
Cuts at: 460 1318 1762 2116 460
Size: 858 444 354 2972
Fragments arranged by size:
2972 858 444 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2211 2344 2803 3012 2211
Size: 133 459 209 3827
Fragments arranged by size:
3827 459 209 133
TatI w'GTAC_w
Cuts at: 4143 4143
Size: 4628
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4145 4177 4145
Size: 32 4596
Tth111I GACn'n_nGTC
Cuts at: 2425 2425
Size: 4628
XbaI T'CTAG_A
Cuts at: 1659 1659
Size: 4628
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2123 2123
Size: 4628
XmaI C'CCGG_G
Cuts at: 135 1648 135
Size: 1513 3115
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NheI NspI PciI PflMI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AlfI AloI ApaI
AscI BaeI BbvCI BfrBI BglI Bpu10I BsgI BsiWI
BsmI BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI HindIII
HpaI MfeI NcoI NotI NruI NsiI PacI PasI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI