Restriction map for
pF1KB5021
(Circular) (Six-base) MAPSORT of: pF1KB5021.seq Check: 225 from: 1 to: 4601
pF1KB5021 4601 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 956 1639 956
Size: 683 3918
Acc65I G'GTAC_C
Cuts at: 1617 1617
Size: 4601
AclI AA'CG_TT
Cuts at: 4441 4441
Size: 4601
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 234 337 515 1118 1562 2426 2858 3211 3873
Size: 103 178 603 444 864 432 353 662
Cuts at: 3873 234
Size: 962
Fragments arranged by size:
962 864 662 603 444 432 353 178
103
AflIII A'CryG_T
Cuts at: 1002 1953 3753 4034 1002
Size: 951 1800 281 1569
Fragments arranged by size:
1800 1569 951 281
AhdI GACnn_n'nnGTC
Cuts at: 1006 1006
Size: 4601
AlwNI CAG_nnn'CTG
Cuts at: 1906 3344 1906
Size: 1438 3163
ApaLI G'TGCA_C
Cuts at: 3439 3439
Size: 4601
ApoI r'AATT_y
Cuts at: 394 664 1343 1605 3954 4210 394
Size: 270 679 262 2349 256 785
Fragments arranged by size:
2349 785 679 270 262 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4601
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4601
AvaI C'yCGr_G
Cuts at: 1621 1621
Size: 4601
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1069 1102 1069
Size: 33 4568
BamHI G'GATC_C
Cuts at: 1626 1626
Size: 4601
BanI G'GyrC_C
Cuts at: 1142 1321 1617 2031 2279 2314 1142
Size: 179 296 414 248 35 3429
Fragments arranged by size:
3429 414 296 248 179 35
BanII G_rGCy'C
Cuts at: 1615 2645 1615
Size: 1030 3571
BbeI G_GCGC'C
Cuts at: 2035 2283 2035
Size: 248 4353
BbsI GAAGACnn'nnnn_
Cuts at: 240 1337 1589 240
Size: 1097 252 3252
Fragments arranged by size:
3252 1097 252
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4567
BciVI GTATCCnnnnn_n'
Cuts at: 428 2499 3555 4601 428
Size: 2071 1056 1046 428
Fragments arranged by size:
2071 1056 1046 428
BclI T'GATC_A
Cuts at: 2121 2121
Size: 4601
BfrBI ATG'CAT
Cuts at: 1549 1549
Size: 4601
BglII A'GATC_T
Cuts at: 2116 4153 2116
Size: 2037 2564
BlpI GC'TnA_GC
Cuts at: 1713 1713
Size: 4601
Bme1580I G_kGCm'C
Cuts at: 2226 2319 3443 2226
Size: 93 1124 3384
Fragments arranged by size:
3384 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1813 1894 2229 4373 1813
Size: 81 335 2144 2041
Fragments arranged by size:
2144 2041 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2968 2968
Size: 4601
Bpu10I CC'TnA_GC
Cuts at: 884 884
Size: 4601
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1777 2656 3370 3668 1777
Size: 879 714 298 2710
Fragments arranged by size:
2710 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4305 4305
Size: 4601
BsaAI yAC'GTr
Cuts at: 1954 1954
Size: 4601
BsaBI GATnn'nnATC
Cuts at: 6 2115 4152 6
Size: 2109 2037 455
Fragments arranged by size:
2109 2037 455
BsaHI Gr'CG_yC
Cuts at: 2032 2280 2982 4478 2032
Size: 248 702 1496 2155
Fragments arranged by size:
2155 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1790 2311 2989 3400 3547 3821 1790
Size: 521 678 411 147 274 2570
Fragments arranged by size:
2570 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 163 1376 2026 2784 3449 3857 163
Size: 1213 650 758 665 408 907
Fragments arranged by size:
1213 907 758 665 650 408
BsiEI CG_ry'CG
Cuts at: 104 2143 2189 3419 104
Size: 2039 46 1230 1286
Fragments arranged by size:
2039 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 749 890 1615 2393 2583 3443 749
Size: 141 725 778 190 860 1907
Fragments arranged by size:
1907 860 778 725 190 141
BsmI GAATG_Cn'
Cuts at: 158 451 1527 1549 158
Size: 293 1076 22 3210
Fragments arranged by size:
3210 1076 293 22
Bsp1286I G_dGCh'C
Cuts at: 749 890 1615 2226 2319 2393 2583 2645 3443
Size: 141 725 611 93 74 190 62 798
Cuts at: 3443 749
Size: 1907
Fragments arranged by size:
1907 798 725 611 190 141 93 74
62
BspHI T'CATG_A
Cuts at: 3033 3033
Size: 4601
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1651 1865 2167 2548 1651
Size: 214 302 381 3704
Fragments arranged by size:
3704 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2893 2947 4431 4596 50
Size: 2843 54 1484 165 55
Fragments arranged by size:
2843 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2513 2513
Size: 4601
BsrFI r'CCGG_y
Cuts at: 2599 2780 2599
Size: 181 4420
BsrGI T'GTAC_A
Cuts at: 1389 1389
Size: 4601
BssSI C'ACGA_G
Cuts at: 2872 3580 2872
Size: 708 3893
BstAPI GCAn_nnn'nTGC
Cuts at: 1546 1978 1546
Size: 432 4169
BstBI TT'CG_AA
Cuts at: 2962 2962
Size: 4601
BstXI CCAn_nnnn'nTGG
Cuts at: 1533 1533
Size: 4601
BstYI r'GATC_y
Cuts at: 1 486 1626 2093 2116 2451 2697 3003 3015
Size: 485 1140 467 23 335 246 306 12
Cuts at: 3015 3101 4153 1
Size: 86 1052 449
Fragments arranged by size:
1140 1052 485 467 449 335 306 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 957 957
Size: 4601
BtgI C'CryG_G
Cuts at: 125 125
Size: 4601
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2551 2732 2895 2551
Size: 181 163 4257
Fragments arranged by size:
4257 181 163
BtsI GCAGTG_nn'
Cuts at: 1844 1844
Size: 4601
DraI TTT'AAA
Cuts at: 290 1600 290
Size: 1310 3291
DrdI GACnn_nn'nnGTC
Cuts at: 2307 3651 2307
Size: 1344 3257
EaeI y'GGCC_r
Cuts at: 2186 2360 2751 2778 4123 2186
Size: 174 391 27 1345 2664
Fragments arranged by size:
2664 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2186 2186
Size: 4601
EarI CTCTTCn'nnn_
Cuts at: 187 2624 2834 187
Size: 2437 210 1954
Fragments arranged by size:
2437 1954 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2809 3551 3697 4411 2809
Size: 742 146 714 2999
Fragments arranged by size:
2999 742 714 146
EcoICRI GAG'CTC
Cuts at: 1613 1613
Size: 4601
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 234 337 515 1118 1562 2426 2858 3211 3873
Size: 103 178 603 444 864 432 353 662
Cuts at: 3873 234
Size: 962
Fragments arranged by size:
962 864 662 603 444 432 353 178
103
EcoO109I rG'GnC_Cy
Cuts at: 1740 1740
Size: 4601
EcoRI G'AATT_C
Cuts at: 394 1605 394
Size: 1211 3390
EcoRV GAT'ATC
Cuts at: 1443 1443
Size: 4601
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1350 1354 2620 1350
Size: 4 1266 3331
Fragments arranged by size:
3331 1266 4
FspI TGC'GCA
Cuts at: 2382 2382
Size: 4601
HaeII r_GCGC'y
Cuts at: 2035 2283 3513 4284 2035
Size: 248 1230 771 2352
Fragments arranged by size:
2352 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 992 1024 2443 2473 2475 2505 992
Size: 32 1419 30 2 30 3088
Fragments arranged by size:
3088 1419 32 30 30 2
HincII GTy'rAC
Cuts at: 1640 1640
Size: 4601
HindIII A'AGCT_T
Cuts at: 811 811
Size: 4601
KasI G'GCGC_C
Cuts at: 2031 2279 2031
Size: 248 4353
KpnI G_GTAC'C
Cuts at: 1621 1621
Size: 4601
MluI A'CGCG_T
Cuts at: 4034 4034
Size: 4601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 759 3360 3544 759
Size: 2601 184 1816
Fragments arranged by size:
2601 1816 184
MscI TGG'CCA
Cuts at: 2362 2362
Size: 4601
MslI CAynn'nnrTG
Cuts at: 440 2590 2717 440
Size: 2150 127 2324
Fragments arranged by size:
2324 2150 127
MspA1I CmG'CkG
Cuts at: 1376 1712 2026 2386 3170 3415 1376
Size: 336 314 360 784 245 2562
Fragments arranged by size:
2562 784 360 336 314 245
NaeI GCC'GGC
Cuts at: 2782 2782
Size: 4601
NarI GG'CG_CC
Cuts at: 2032 2280 2032
Size: 248 4353
NcoI C'CATG_G
Cuts at: 125 125
Size: 4601
NgoMIV G'CCGG_C
Cuts at: 2780 2780
Size: 4601
NsiI A_TGCA'T
Cuts at: 1551 1551
Size: 4601
NspI r_CATG'y
Cuts at: 1006 1654 2154 3757 1006
Size: 648 500 1603 1850
Fragments arranged by size:
1850 1603 648 500
PciI A'CATG_T
Cuts at: 1002 3753 1002
Size: 2751 1850
PmeI GTTT'AAAC
Cuts at: 1600 1600
Size: 4601
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3047 3079 3047
Size: 32 4569
PsiI TTA'TAA
Cuts at: 209 281 431 209
Size: 72 150 4379
Fragments arranged by size:
4379 150 72
PstI C_TGCA'G
Cuts at: 1648 2333 1648
Size: 685 3916
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4601
PvuII CAG'CTG
Cuts at: 1376 2026 2386 1376
Size: 650 360 3591
Fragments arranged by size:
3591 650 360
RsrII CG'GwC_CG
Cuts at: 2796 2796
Size: 4601
SacI G_AGCT'C
Cuts at: 1615 1615
Size: 4601
SalI G'TCGA_C
Cuts at: 1638 1638
Size: 4601
SapI GCTCTTCn'nnn_
Cuts at: 2624 2834 2624
Size: 210 4391
SbfI CC_TGCA'GG
Cuts at: 1648 1648
Size: 4601
ScaI AGT'ACT
Cuts at: 4118 4118
Size: 4601
SfcI C'TryA_G
Cuts at: 33 1130 1514 1644 2329 3297 3488 33
Size: 1097 384 130 685 968 191 1146
Fragments arranged by size:
1146 1097 968 685 384 191 130
SfoI GGC'GCC
Cuts at: 2033 2281 2033
Size: 248 4353
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4601
SmaI CCC'GGG
Cuts at: 1623 1623
Size: 4601
SmlI C'TyrA_G
Cuts at: 1756 2671 3385 3647 1756
Size: 915 714 262 2710
Fragments arranged by size:
2710 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4601
SphI G_CATG'C
Cuts at: 1654 2154 1654
Size: 500 4101
SspI AAT'ATT
Cuts at: 338 981 338
Size: 643 3958
StyI C'CwwG_G
Cuts at: 125 1146 1325 1735 2089 125
Size: 1021 179 410 354 2637
Fragments arranged by size:
2637 1021 410 354 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2184 2317 2776 2985 2184
Size: 133 459 209 3800
Fragments arranged by size:
3800 459 209 133
TatI w'GTAC_w
Cuts at: 1389 4116 1389
Size: 2727 1874
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4118 4150 4118
Size: 32 4569
Tth111I GACn'n_nGTC
Cuts at: 2398 2398
Size: 4601
XbaI T'CTAG_A
Cuts at: 1632 1632
Size: 4601
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2096 2096
Size: 4601
XmaI C'CCGG_G
Cuts at: 1621 1621
Size: 4601
XmnI GAAnn'nnTTC
Cuts at: 398 980 1334 1478 398
Size: 582 354 144 3521
Fragments arranged by size:
3521 582 354 144
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AloI
ApaI AscI AvrII BbvCI BglI BmgBI BmtI BpmI
BsaXI BseRI BsgI BsiWI BsmBI BspEI BssHII BstEII
Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI HpaI
MfeI NdeI NheI NotI NruI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI