Restriction map for
pF1KB5023
(Circular) (Six-base) MAPSORT of: pF1KB5023.seq Check: 6870 from: 1 to: 4100
pF1KB5023 4100 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 1138 1138
Size: 4100
Acc65I G'GTAC_C
Cuts at: 1116 1116
Size: 4100
AclI AA'CG_TT
Cuts at: 3940 3940
Size: 4100
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1925 2357 3104 3372 1925
Size: 432 747 268 2653
Fragments arranged by size:
2653 747 432 268
AflIII A'CryG_T
Cuts at: 1452 2556 3533 1452
Size: 1104 977 2019
Fragments arranged by size:
2019 1104 977
AgeI A'CCGG_T
Cuts at: 2488 2488
Size: 4100
AhdI GACnn_n'nnGTC
Cuts at: 166 166
Size: 4100
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 545 577 545
Size: 32 4068
AlwNI CAG_nnn'CTG
Cuts at: 539 1405 2972 539
Size: 866 1567 1667
Fragments arranged by size:
1667 1567 866
ApaLI G'TGCA_C
Cuts at: 2870 2870
Size: 4100
ApoI r'AATT_y
Cuts at: 151 940 1104 3453 3709 151
Size: 789 164 2349 256 542
Fragments arranged by size:
2349 789 542 256 164
AseI AT'TA_AT
Cuts at: 20 20
Size: 4100
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4100
AvaI C'yCGr_G
Cuts at: 1120 1120
Size: 4100
BamHI G'GATC_C
Cuts at: 1125 1125
Size: 4100
BanI G'GyrC_C
Cuts at: 1116 1530 1778 1813 1116
Size: 414 248 35 3403
Fragments arranged by size:
3403 414 248 35
BanII G_rGCy'C
Cuts at: 525 690 1114 2144 525
Size: 165 424 1030 2481
Fragments arranged by size:
2481 1030 424 165
BbeI G_GCGC'C
Cuts at: 1534 1782 1534
Size: 248 3852
BbsI GAAGACnn'nnnn_
Cuts at: 805 805
Size: 4100
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 878 912 878
Size: 34 4066
BciVI GTATCCnnnnn_n'
Cuts at: 1998 2759 4100 1998
Size: 761 1341 1998
Fragments arranged by size:
1998 1341 761
BclI T'GATC_A
Cuts at: 1620 1620
Size: 4100
BfrBI ATG'CAT
Cuts at: 238 358 238
Size: 120 3980
BglII A'GATC_T
Cuts at: 1615 3652 1615
Size: 2037 2063
BlpI GC'TnA_GC
Cuts at: 1212 1212
Size: 4100
Bme1580I G_kGCm'C
Cuts at: 643 1725 1818 2874 643
Size: 1082 93 1056 1869
Fragments arranged by size:
1869 1082 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1312 1393 1728 3872 1312
Size: 81 335 2144 1540
Fragments arranged by size:
2144 1540 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 934 2467 934
Size: 1533 2567
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 572 572
Size: 4100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 828 1276 2155 2647 2945 828
Size: 448 879 492 298 1983
Fragments arranged by size:
1983 879 492 448 298
BsaI GGTCTCn'nnnn_
Cuts at: 3804 3804
Size: 4100
BsaAI yAC'GTr
Cuts at: 1453 1453
Size: 4100
BsaBI GATnn'nnATC
Cuts at: 6 1614 3651 6
Size: 1608 2037 455
Fragments arranged by size:
2037 1608 455
BsaHI Gr'CG_yC
Cuts at: 1531 1779 2481 3977 1531
Size: 248 702 1496 1654
Fragments arranged by size:
1654 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1289 1810 2488 2762 2909 3320 1289
Size: 521 678 274 147 411 2069
Fragments arranged by size:
2069 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 545 575 818 848 545
Size: 30 243 30 3797
Fragments arranged by size:
3797 243 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 599 599
Size: 4100
BseYI C'CCAG_C
Cuts at: 328 1525 2283 2860 3356 328
Size: 1197 758 577 496 1072
Fragments arranged by size:
1197 1072 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1642 1688 2896 104
Size: 1538 46 1208 1308
Fragments arranged by size:
1538 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 525 1114 1892 2082 2874 525
Size: 589 778 190 792 1751
Fragments arranged by size:
1751 792 778 589 190
BsmI GAATG_Cn'
Cuts at: 497 497
Size: 4100
Bsp1286I G_dGCh'C
Cuts at: 525 643 690 1114 1725 1818 1892 2082 2144
Size: 118 47 424 611 93 74 190 62
Cuts at: 2144 2874 525
Size: 730 1751
Fragments arranged by size:
1751 730 611 424 190 118 93 74
62 47
BspEI T'CCGG_A
Cuts at: 3320 3320
Size: 4100
BspHI T'CATG_A
Cuts at: 1041 3276 1041
Size: 2235 1865
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1150 1364 1666 2047 1150
Size: 214 302 381 3203
Fragments arranged by size:
3203 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2392 2446 3930 4095 50
Size: 2342 54 1484 165 55
Fragments arranged by size:
2342 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2012 2012
Size: 4100
BsrFI r'CCGG_y
Cuts at: 2098 2279 2488 2098
Size: 181 209 3710
Fragments arranged by size:
3710 209 181
BssSI C'ACGA_G
Cuts at: 875 2371 2729 875
Size: 1496 358 2246
Fragments arranged by size:
2246 1496 358
BstAPI GCAn_nnn'nTGC
Cuts at: 424 1477 424
Size: 1053 3047
BstBI TT'CG_AA
Cuts at: 2461 2461
Size: 4100
BstXI CCAn_nnnn'nTGG
Cuts at: 241 241
Size: 4100
BstYI r'GATC_y
Cuts at: 1 1125 1592 1615 1950 2196 3208 3294 3306
Size: 1124 467 23 335 246 1012 86 12
Cuts at: 3306 3652 1
Size: 346 449
Fragments arranged by size:
1124 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 254 884 107
Size: 147 630 3323
Fragments arranged by size:
3323 630 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2050 2231 2394 2050
Size: 181 163 3756
Fragments arranged by size:
3756 181 163
BtsI GCAGTG_nn'
Cuts at: 641 1343 641
Size: 702 3398
DraI TTT'AAA
Cuts at: 477 1099 477
Size: 622 3478
DrdI GACnn_nn'nnGTC
Cuts at: 1806 2664 1806
Size: 858 3242
EaeI y'GGCC_r
Cuts at: 1031 1685 1859 2250 2277 3622 1031
Size: 654 174 391 27 1345 1509
Fragments arranged by size:
1509 1345 654 391 174 27
EagI C'GGCC_G
Cuts at: 1685 1685
Size: 4100
EarI CTCTTCn'nnn_
Cuts at: 2123 2333 2123
Size: 210 3890
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2308 2618 2764 3910 2308
Size: 310 146 1146 2498
Fragments arranged by size:
2498 1146 310 146
EcoICRI GAG'CTC
Cuts at: 523 1112 523
Size: 589 3511
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 572 1925 2357 3104 3372 572
Size: 1353 432 747 268 1300
Fragments arranged by size:
1353 1300 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1001 1001
Size: 4100
EcoO109I rG'GnC_Cy
Cuts at: 1239 1239
Size: 4100
EcoRI G'AATT_C
Cuts at: 1104 1104
Size: 4100
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2119 2119
Size: 4100
FspI TGC'GCA
Cuts at: 1881 1881
Size: 4100
HaeII r_GCGC'y
Cuts at: 1534 1782 2804 3783 1534
Size: 248 1022 979 1851
Fragments arranged by size:
1851 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 152 184 343 375 476 508 512 544 993
Size: 32 159 32 101 32 4 32 449
Cuts at: 993 1025 1942 1972 1974 2004 152
Size: 32 917 30 2 30 2248
Fragments arranged by size:
2248 917 449 159 101 32 32 32
32 32 30 30 4 2
HincII GTy'rAC
Cuts at: 249 1139 249
Size: 890 3210
KasI G'GCGC_C
Cuts at: 1530 1778 1530
Size: 248 3852
KpnI G_GTAC'C
Cuts at: 1120 1120
Size: 4100
MluI A'CGCG_T
Cuts at: 3533 3533
Size: 4100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 327 570 2771 2955 327
Size: 243 2201 184 1472
Fragments arranged by size:
2201 1472 243 184
MscI TGG'CCA
Cuts at: 1861 1861
Size: 4100
MslI CAynn'nnrTG
Cuts at: 239 1061 2089 2216 239
Size: 822 1028 127 2123
Fragments arranged by size:
2123 1028 822 127
MspA1I CmG'CkG
Cuts at: 1211 1525 1885 2898 3143 1211
Size: 314 360 1013 245 2168
Fragments arranged by size:
2168 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2281 2281
Size: 4100
NarI GG'CG_CC
Cuts at: 1531 1779 1531
Size: 248 3852
NcoI C'CATG_G
Cuts at: 107 884 107
Size: 777 3323
NgoMIV G'CCGG_C
Cuts at: 2279 2279
Size: 4100
NsiI A_TGCA'T
Cuts at: 240 360 240
Size: 120 3980
NspI r_CATG'y
Cuts at: 242 605 1153 1653 2560 242
Size: 363 548 500 907 1782
Fragments arranged by size:
1782 907 548 500 363
PciI A'CATG_T
Cuts at: 2556 2556
Size: 4100
PmeI GTTT'AAAC
Cuts at: 1099 1099
Size: 4100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 545 577 3239 3271 545
Size: 32 2662 32 1374
Fragments arranged by size:
2662 1374 32 32
PshAI GACnn'nnGTC
Cuts at: 809 809
Size: 4100
PstI C_TGCA'G
Cuts at: 1147 1832 1147
Size: 685 3415
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4100
PvuII CAG'CTG
Cuts at: 1525 1885 1525
Size: 360 3740
RsrII CG'GwC_CG
Cuts at: 2295 2295
Size: 4100
SacI G_AGCT'C
Cuts at: 525 1114 525
Size: 589 3511
SalI G'TCGA_C
Cuts at: 1137 1137
Size: 4100
SapI GCTCTTCn'nnn_
Cuts at: 2123 2333 2123
Size: 210 3890
SbfI CC_TGCA'GG
Cuts at: 1147 1147
Size: 4100
ScaI AGT'ACT
Cuts at: 3617 3617
Size: 4100
SfcI C'TryA_G
Cuts at: 33 1143 1828 2821 3012 33
Size: 1110 685 993 191 1121
Fragments arranged by size:
1121 1110 993 685 191
SfoI GGC'GCC
Cuts at: 1532 1780 1532
Size: 248 3852
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4100
SmaI CCC'GGG
Cuts at: 1122 1122
Size: 4100
SmlI C'TyrA_G
Cuts at: 807 1255 2170 2662 2924 807
Size: 448 915 492 262 1983
Fragments arranged by size:
1983 915 492 448 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4100
SphI G_CATG'C
Cuts at: 605 1153 1653 605
Size: 548 500 3052
Fragments arranged by size:
3052 548 500
StyI C'CwwG_G
Cuts at: 107 884 932 1234 1588 107
Size: 777 48 302 354 2619
Fragments arranged by size:
2619 777 354 302 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1683 1816 2275 2484 1683
Size: 133 459 209 3299
Fragments arranged by size:
3299 459 209 133
TatI w'GTAC_w
Cuts at: 3615 3615
Size: 4100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 818 850 3617 3649 818
Size: 32 2767 32 1269
Fragments arranged by size:
2767 1269 32 32
Tth111I GACn'n_nGTC
Cuts at: 1897 1897
Size: 4100
XbaI T'CTAG_A
Cuts at: 1131 1131
Size: 4100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1595 1595
Size: 4100
XmaI C'CCGG_G
Cuts at: 1120 1120
Size: 4100
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PmeI PpiI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI ApaI AscI
AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I BsgI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PasI PflMI PfoI
PmlI PpuMI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI