Restriction map for
pF1KB5024
(Circular) (Six-base) MAPSORT of: pF1KB5024.seq Check: 724 from: 1 to: 4397
pF1KB5024 4397 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1377 1377
Size: 4397
AccI GT'mk_AC
Cuts at: 1435 1435
Size: 4397
Acc65I G'GTAC_C
Cuts at: 1413 1413
Size: 4397
AclI AA'CG_TT
Cuts at: 4237 4237
Size: 4397
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 768 2222 2654 3401 3669 768
Size: 1454 432 747 268 1496
Fragments arranged by size:
1496 1454 747 432 268
AflIII A'CryG_T
Cuts at: 188 575 1749 2853 3830 188
Size: 387 1174 1104 977 755
Fragments arranged by size:
1174 1104 977 755 387
AgeI A'CCGG_T
Cuts at: 2785 2785
Size: 4397
AleI CACnn'nnGTG
Cuts at: 1339 1381 1339
Size: 42 4355
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 656 656
Size: 4397
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 706 738 706
Size: 32 4365
AlwNI CAG_nnn'CTG
Cuts at: 381 426 526 1054 1120 1702 3269 381
Size: 45 100 528 66 582 1567 1509
Fragments arranged by size:
1567 1509 582 528 100 66 45
ApaI G_GGCC'C
Cuts at: 134 846 134
Size: 712 3685
ApaLI G'TGCA_C
Cuts at: 3167 3167
Size: 4397
ApoI r'AATT_y
Cuts at: 1401 3750 4006 1401
Size: 2349 256 1792
Fragments arranged by size:
2349 1792 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4397
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4397
AvaI C'yCGr_G
Cuts at: 451 1417 451
Size: 966 3431
BamHI G'GATC_C
Cuts at: 1422 1422
Size: 4397
BanI G'GyrC_C
Cuts at: 1413 1827 2075 2110 1413
Size: 414 248 35 3700
Fragments arranged by size:
3700 414 248 35
BanII G_rGCy'C
Cuts at: 134 604 846 953 1411 2441 134
Size: 470 242 107 458 1030 2090
Fragments arranged by size:
2090 1030 470 458 242 107
BbeI G_GCGC'C
Cuts at: 1831 2079 1831
Size: 248 4149
BbvCI CC'TCA_GC
Cuts at: 556 812 556
Size: 256 4141
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1297 1331 1297
Size: 34 4363
BciVI GTATCCnnnnn_n'
Cuts at: 2295 3056 4397 2295
Size: 761 1341 2295
Fragments arranged by size:
2295 1341 761
BclI T'GATC_A
Cuts at: 1364 1917 1364
Size: 553 3844
BglI GCCn_nnn'nGGC
Cuts at: 542 823 919 1177 542
Size: 281 96 258 3762
Fragments arranged by size:
3762 281 258 96
BglII A'GATC_T
Cuts at: 1912 3949 1912
Size: 2037 2360
BlpI GC'TnA_GC
Cuts at: 1121 1509 1121
Size: 388 4009
Bme1580I G_kGCm'C
Cuts at: 134 846 2022 2115 3171 134
Size: 712 1176 93 1056 1360
Fragments arranged by size:
1360 1176 1056 712 93
BmgBI CAC'GTC
Cuts at: 576 576
Size: 4397
BmrI ACTGGGnnnn_n'
Cuts at: 813 1609 1690 2025 4169 813
Size: 796 81 335 2144 1041
Fragments arranged by size:
2144 1041 796 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2764 2764
Size: 4397
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 448
Size: 4397
Bpu10I CC'TnA_GC
Cuts at: 556 812 556
Size: 256 4141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1120 1573 2452 2944 3242 1120
Size: 453 879 492 298 2275
Fragments arranged by size:
2275 879 492 453 298
BsaI GGTCTCn'nnnn_
Cuts at: 4101 4101
Size: 4397
BsaAI yAC'GTr
Cuts at: 189 1750 189
Size: 1561 2836
BsaBI GATnn'nnATC
Cuts at: 6 1911 3948 6
Size: 1905 2037 455
Fragments arranged by size:
2037 1905 455
BsaHI Gr'CG_yC
Cuts at: 1828 2076 2778 4274 1828
Size: 248 702 1496 1951
Fragments arranged by size:
1951 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1586 2107 2785 3059 3206 3617 1586
Size: 521 678 274 147 411 2366
Fragments arranged by size:
2366 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 805 805
Size: 4397
BseYI C'CCAG_C
Cuts at: 200 1822 2580 3157 3653 200
Size: 1622 758 577 496 944
Fragments arranged by size:
1622 944 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 652 913 1405 415
Size: 237 261 492 3407
Fragments arranged by size:
3407 492 261 237
BsiEI CG_ry'CG
Cuts at: 104 293 1939 1985 3193 104
Size: 189 1646 46 1208 1308
Fragments arranged by size:
1646 1308 1208 189 46
BsiHKAI G_wGCw'C
Cuts at: 604 953 1411 2189 2379 3171 604
Size: 349 458 778 190 792 1830
Fragments arranged by size:
1830 792 778 458 349 190
BsiWI C'GTAC_G
Cuts at: 937 937
Size: 4397
BsmBI CGTCTCn'nnnn_
Cuts at: 280 280
Size: 4397
Bsp1286I G_dGCh'C
Cuts at: 134 604 846 953 1411 2022 2115 2189 2379
Size: 470 242 107 458 611 93 74 190
Cuts at: 2379 2441 3171 134
Size: 62 730 1360
Fragments arranged by size:
1360 730 611 470 458 242 190 107
93 74 62
BspEI T'CCGG_A
Cuts at: 3617 3617
Size: 4397
BspHI T'CATG_A
Cuts at: 338 3573 338
Size: 3235 1162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1020 1377 1447 1661 1963 2344 1020
Size: 357 70 214 302 381 3073
Fragments arranged by size:
3073 381 357 302 214 70
BsrBI CCG'CTC
Cuts at: 50 1125 2689 2743 4227 4392 50
Size: 1075 1564 54 1484 165 55
Fragments arranged by size:
1564 1484 1075 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2309 2309
Size: 4397
BsrFI r'CCGG_y
Cuts at: 968 2395 2576 2785 968
Size: 1427 181 209 2580
Fragments arranged by size:
2580 1427 209 181
BssSI C'ACGA_G
Cuts at: 2668 3026 2668
Size: 358 4039
BstAPI GCAn_nnn'nTGC
Cuts at: 1774 1774
Size: 4397
BstBI TT'CG_AA
Cuts at: 2758 2758
Size: 4397
BstYI r'GATC_y
Cuts at: 1 551 860 929 1061 1422 1889 1912 2247
Size: 550 309 69 132 361 467 23 335
Cuts at: 2247 2493 3505 3591 3603 3949 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 550 467 449 361 346 335 309
246 132 86 69 23 12
BtgI C'CryG_G
Cuts at: 107 362 626 914 107
Size: 255 264 288 3590
Fragments arranged by size:
3590 288 264 255
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 939 1117 2347 2528 2691 939
Size: 178 1230 181 163 2645
Fragments arranged by size:
2645 1230 181 178 163
BtsI GCAGTG_nn'
Cuts at: 1640 1640
Size: 4397
DraI TTT'AAA
Cuts at: 1396 1396
Size: 4397
DraIII CAC_nnn'GTG
Cuts at: 126 126
Size: 4397
DrdI GACnn_nn'nnGTC
Cuts at: 2103 2961 2103
Size: 858 3539
EaeI y'GGCC_r
Cuts at: 290 359 534 656 1304 1982 2156 2547 2574
Size: 69 175 122 648 678 174 391 27
Cuts at: 2574 3919 290
Size: 1345 768
Fragments arranged by size:
1345 768 678 648 391 175 174 122
69 27
EagI C'GGCC_G
Cuts at: 290 1982 290
Size: 1692 2705
EarI CTCTTCn'nnn_
Cuts at: 866 2420 2630 866
Size: 1554 210 2633
Fragments arranged by size:
2633 1554 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1096 2605 2915 3061 4207 1096
Size: 1509 310 146 1146 1286
Fragments arranged by size:
1509 1286 1146 310 146
EcoICRI GAG'CTC
Cuts at: 602 951 1409 602
Size: 349 458 3590
Fragments arranged by size:
3590 458 349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 768 2222 2654 3401 3669 448
Size: 320 1454 432 747 268 1176
Fragments arranged by size:
1454 1176 747 432 320 268
EcoO109I rG'GnC_Cy
Cuts at: 130 455 842 1032 1194 1536 130
Size: 325 387 190 162 342 2991
Fragments arranged by size:
2991 387 342 325 190 162
EcoRI G'AATT_C
Cuts at: 1401 1401
Size: 4397
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 777 798 1197 2416 777
Size: 21 399 1219 2758
Fragments arranged by size:
2758 1219 399 21
FspI TGC'GCA
Cuts at: 2178 2178
Size: 4397
HaeII r_GCGC'y
Cuts at: 145 1831 2079 3101 4080 145
Size: 1686 248 1022 979 462
Fragments arranged by size:
1686 1022 979 462 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2239 2269 2271 2301 2239
Size: 30 2 30 4335
Fragments arranged by size:
4335 30 30 2
HincII GTy'rAC
Cuts at: 798 1436 798
Size: 638 3759
HpaI GTT'AAC
Cuts at: 798 798
Size: 4397
KasI G'GCGC_C
Cuts at: 1827 2075 1827
Size: 248 4149
KpnI G_GTAC'C
Cuts at: 1417 1417
Size: 4397
MfeI C'AATT_G
Cuts at: 114 114
Size: 4397
MluI A'CGCG_T
Cuts at: 3830 3830
Size: 4397
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 197 251 3068 3252 197
Size: 54 2817 184 1342
Fragments arranged by size:
2817 1342 184 54
MscI TGG'CCA
Cuts at: 2158 2158
Size: 4397
MslI CAynn'nnrTG
Cuts at: 652 1339 1381 2386 2513 652
Size: 687 42 1005 127 2536
Fragments arranged by size:
2536 1005 687 127 42
MspA1I CmG'CkG
Cuts at: 272 811 1508 1822 2182 3195 3440 272
Size: 539 697 314 360 1013 245 1229
Fragments arranged by size:
1229 1013 697 539 360 314 245
NaeI GCC'GGC
Cuts at: 970 2578 970
Size: 1608 2789
NarI GG'CG_CC
Cuts at: 1828 2076 1828
Size: 248 4149
NcoI C'CATG_G
Cuts at: 107 107
Size: 4397
NgoMIV G'CCGG_C
Cuts at: 968 2576 968
Size: 1608 2789
NspI r_CATG'y
Cuts at: 1330 1450 1950 2857 1330
Size: 120 500 907 2870
Fragments arranged by size:
2870 907 500 120
PasI CC'CwG_GG
Cuts at: 606 606
Size: 4397
PciI A'CATG_T
Cuts at: 2853 2853
Size: 4397
PflMI CCAn_nnn'nTGG
Cuts at: 126 126
Size: 4397
PfoI T'CCnGG_A
Cuts at: 683 683
Size: 4397
PmeI GTTT'AAAC
Cuts at: 1396 1396
Size: 4397
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3536 3568 3536
Size: 32 4365
PpuMI rG'GwC_Cy
Cuts at: 455 1032 455
Size: 577 3820
PshAI GACnn'nnGTC
Cuts at: 319 319
Size: 4397
PspOMI G'GGCC_C
Cuts at: 130 842 130
Size: 712 3685
PstI C_TGCA'G
Cuts at: 422 839 1444 2129 422
Size: 417 605 685 2690
Fragments arranged by size:
2690 685 605 417
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4397
PvuII CAG'CTG
Cuts at: 811 1822 2182 811
Size: 1011 360 3026
Fragments arranged by size:
3026 1011 360
RsrII CG'GwC_CG
Cuts at: 2592 2592
Size: 4397
SacI G_AGCT'C
Cuts at: 604 953 1411 604
Size: 349 458 3590
Fragments arranged by size:
3590 458 349
SalI G'TCGA_C
Cuts at: 1434 1434
Size: 4397
SanDI GG'GwC_CC
Cuts at: 455 455
Size: 4397
SapI GCTCTTCn'nnn_
Cuts at: 2420 2630 2420
Size: 210 4187
SbfI CC_TGCA'GG
Cuts at: 1444 1444
Size: 4397
ScaI AGT'ACT
Cuts at: 3914 3914
Size: 4397
SfcI C'TryA_G
Cuts at: 33 418 835 959 1440 2125 3118 3309 33
Size: 385 417 124 481 685 993 191 1121
Fragments arranged by size:
1121 993 685 481 417 385 191 124
SfiI GGCCn_nnn'nGGCC
Cuts at: 823 823
Size: 4397
SfoI GGC'GCC
Cuts at: 1829 2077 1829
Size: 248 4149
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4397
SmaI CCC'GGG
Cuts at: 1419 1419
Size: 4397
SmlI C'TyrA_G
Cuts at: 1135 1552 2467 2959 3221 1135
Size: 417 915 492 262 2311
Fragments arranged by size:
2311 915 492 417 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4397
SphI G_CATG'C
Cuts at: 1330 1450 1950 1330
Size: 120 500 3777
Fragments arranged by size:
3777 500 120
StyI C'CwwG_G
Cuts at: 107 1181 1235 1531 1885 107
Size: 1074 54 296 354 2619
Fragments arranged by size:
2619 1074 354 296 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1980 2113 2572 2781 1980
Size: 133 459 209 3596
Fragments arranged by size:
3596 459 209 133
TatI w'GTAC_w
Cuts at: 3912 3912
Size: 4397
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 679 711 914 946 3914 3946 679
Size: 32 203 32 2968 32 1130
Fragments arranged by size:
2968 1130 203 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2194 2194
Size: 4397
XbaI T'CTAG_A
Cuts at: 1428 1428
Size: 4397
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 141 1179 1892 141
Size: 1038 713 2646
Fragments arranged by size:
2646 1038 713
XmaI C'CCGG_G
Cuts at: 1417 1417
Size: 4397
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AscI AvrII BaeI BbsI
BfrBI BmtI BsaXI BsmI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII NdeI NheI NotI NruI NsiI PacI PmlI
PsiI PspXI PsrI SacII SexAI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI