Restriction map for
pF1KB5026
(Circular) (Six-base) MAPSORT of: pF1KB5026.seq Check: 3333 from: 1 to: 4457
pF1KB5026 4457 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 647 1495 647
Size: 848 3609
Acc65I G'GTAC_C
Cuts at: 1473 1473
Size: 4457
AclI AA'CG_TT
Cuts at: 4297 4297
Size: 4457
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 287 487 706 1358 2282 2714 3067 3729
Size: 74 200 219 652 924 432 353 662
Cuts at: 3729 213
Size: 941
Fragments arranged by size:
941 924 662 652 432 353 219 200
74
AflIII A'CryG_T
Cuts at: 879 1809 3609 3890 879
Size: 930 1800 281 1446
Fragments arranged by size:
1800 1446 930 281
AlwNI CAG_nnn'CTG
Cuts at: 156 261 1221 1336 1762 3200 156
Size: 105 960 115 426 1438 1413
Fragments arranged by size:
1438 1413 960 426 115 105
ApaI G_GGCC'C
Cuts at: 1448 1448
Size: 4457
ApaLI G'TGCA_C
Cuts at: 205 3295 205
Size: 3090 1367
ApoI r'AATT_y
Cuts at: 749 832 1035 1109 1461 3810 4066 749
Size: 83 203 74 352 2349 256 1140
Fragments arranged by size:
2349 1140 352 256 203 83 74
AseI AT'TA_AT
Cuts at: 20 20
Size: 4457
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4457
AvaI C'yCGr_G
Cuts at: 479 666 1477 479
Size: 187 811 3459
Fragments arranged by size:
3459 811 187
BamHI G'GATC_C
Cuts at: 1482 1482
Size: 4457
BanI G'GyrC_C
Cuts at: 1077 1473 1887 2135 2170 1077
Size: 396 414 248 35 3364
Fragments arranged by size:
3364 414 396 248 35
BanII G_rGCy'C
Cuts at: 1448 1471 2501 1448
Size: 23 1030 3404
Fragments arranged by size:
3404 1030 23
BbeI G_GCGC'C
Cuts at: 1081 1891 2139 1081
Size: 810 248 3399
Fragments arranged by size:
3399 810 248
BbsI GAAGACnn'nnnn_
Cuts at: 542 542
Size: 4457
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4423
BciVI GTATCCnnnnn_n'
Cuts at: 2355 3411 4457 2355
Size: 1056 1046 2355
Fragments arranged by size:
2355 1056 1046
BclI T'GATC_A
Cuts at: 1977 1977
Size: 4457
BglII A'GATC_T
Cuts at: 1972 4009 1972
Size: 2037 2420
BlpI GC'TnA_GC
Cuts at: 1569 1569
Size: 4457
Bme1580I G_kGCm'C
Cuts at: 209 1448 2082 2175 3299 209
Size: 1239 634 93 1124 1367
Fragments arranged by size:
1367 1239 1124 634 93
BmgBI CAC'GTC
Cuts at: 957 957
Size: 4457
BmrI ACTGGGnnnn_n'
Cuts at: 1669 1750 2085 4229 1669
Size: 81 335 2144 1897
Fragments arranged by size:
2144 1897 335 81
BmtI G_CTAG'C
Cuts at: 121 121
Size: 4457
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2824 2824
Size: 4457
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1146 1198 1441 1146
Size: 52 243 4162
Fragments arranged by size:
4162 243 52
Bpu10I CC'TnA_GC
Cuts at: 1131 1131
Size: 4457
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1633 2512 3226 3524 1633
Size: 879 714 298 2566
Fragments arranged by size:
2566 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4161 4161
Size: 4457
BsaAI yAC'GTr
Cuts at: 1810 1810
Size: 4457
BsaBI GATnn'nnATC
Cuts at: 6 308 1971 4008 6
Size: 302 1663 2037 455
Fragments arranged by size:
2037 1663 455 302
BsaHI Gr'CG_yC
Cuts at: 1078 1888 2136 2838 4334 1078
Size: 810 248 702 1496 1201
Fragments arranged by size:
1496 1201 810 702 248
BsaWI w'CCGG_w
Cuts at: 1646 2167 2845 3256 3403 3677 1646
Size: 521 678 411 147 274 2426
Fragments arranged by size:
2426 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 629 659 629
Size: 30 4427
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1438 1438
Size: 4457
BseYI C'CCAG_C
Cuts at: 507 1882 2640 3305 3713 507
Size: 1375 758 665 408 1251
Fragments arranged by size:
1375 1251 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1387 1387
Size: 4457
BsiEI CG_ry'CG
Cuts at: 104 1999 2045 3275 104
Size: 1895 46 1230 1286
Fragments arranged by size:
1895 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 209 1471 2249 2439 3299 209
Size: 1262 778 190 860 1367
Fragments arranged by size:
1367 1262 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 947 947
Size: 4457
Bsp1286I G_dGCh'C
Cuts at: 209 1448 1471 2082 2175 2249 2439 2501 3299
Size: 1239 23 611 93 74 190 62 798
Cuts at: 3299 209
Size: 1367
Fragments arranged by size:
1367 1239 798 611 190 93 74 62
23
BspHI T'CATG_A
Cuts at: 2889 2889
Size: 4457
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1507 1721 2023 2404 1507
Size: 214 302 381 3560
Fragments arranged by size:
3560 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2749 2803 4287 4452 50
Size: 2699 54 1484 165 55
Fragments arranged by size:
2699 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 353 2369 353
Size: 2016 2441
BsrFI r'CCGG_y
Cuts at: 2455 2636 2455
Size: 181 4276
BsrGI T'GTAC_A
Cuts at: 826 1284 826
Size: 458 3999
BssSI C'ACGA_G
Cuts at: 2728 3436 2728
Size: 708 3749
BstAPI GCAn_nnn'nTGC
Cuts at: 1834 1834
Size: 4457
BstBI TT'CG_AA
Cuts at: 2818 2818
Size: 4457
BstXI CCAn_nnnn'nTGG
Cuts at: 375 375
Size: 4457
BstYI r'GATC_y
Cuts at: 1 173 847 1198 1482 1949 1972 2307 2553
Size: 172 674 351 284 467 23 335 246
Cuts at: 2553 2859 2871 2957 4009 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 674 467 449 351 335 306 284
246 172 86 23 12
BtgI C'CryG_G
Cuts at: 140 1391 140
Size: 1251 3206
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2407 2588 2751 2407
Size: 181 163 4113
Fragments arranged by size:
4113 181 163
BtsI GCAGTG_nn'
Cuts at: 1231 1700 1231
Size: 469 3988
DraI TTT'AAA
Cuts at: 938 1456 938
Size: 518 3939
DraIII CAC_nnn'GTG
Cuts at: 1301 1301
Size: 4457
DrdI GACnn_nn'nnGTC
Cuts at: 2163 3507 2163
Size: 1344 3113
EaeI y'GGCC_r
Cuts at: 582 2042 2216 2607 2634 3979 582
Size: 1460 174 391 27 1345 1060
Fragments arranged by size:
1460 1345 1060 391 174 27
EagI C'GGCC_G
Cuts at: 2042 2042
Size: 4457
EarI CTCTTCn'nnn_
Cuts at: 232 2480 2690 232
Size: 2248 210 1999
Fragments arranged by size:
2248 1999 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1446 2665 3407 3553 4267 1446
Size: 1219 742 146 714 1636
Fragments arranged by size:
1636 1219 742 714 146
EcoICRI GAG'CTC
Cuts at: 1469 1469
Size: 4457
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 287 487 706 1146 1198 1358 1441 2282
Size: 74 200 219 440 52 160 83 841
Cuts at: 2282 2714 3067 3729 213
Size: 432 353 662 941
Fragments arranged by size:
941 841 662 440 432 353 219 200
160 83 74 52
EcoO109I rG'GnC_Cy
Cuts at: 1444 1445 1596 1444
Size: 1 151 4305
Fragments arranged by size:
4305 151 1
EcoRI G'AATT_C
Cuts at: 832 1035 1109 1461 832
Size: 203 74 352 3828
Fragments arranged by size:
3828 352 203 74
EcoRV GAT'ATC
Cuts at: 610 610
Size: 4457
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2476 2476
Size: 4457
FspI TGC'GCA
Cuts at: 2238 2238
Size: 4457
HaeII r_GCGC'y
Cuts at: 1081 1420 1891 2139 3369 4140 1081
Size: 339 471 248 1230 771 1398
Fragments arranged by size:
1398 1230 771 471 339 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 629 661 832 864 2299 2329 2331 2361 629
Size: 32 171 32 1435 30 2 30 2725
Fragments arranged by size:
2725 1435 171 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1496 1496
Size: 4457
KasI G'GCGC_C
Cuts at: 1077 1887 2135 1077
Size: 810 248 3399
Fragments arranged by size:
3399 810 248
KpnI G_GTAC'C
Cuts at: 1477 1477
Size: 4457
MluI A'CGCG_T
Cuts at: 3890 3890
Size: 4457
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1038 1242 3216 3400 1038
Size: 204 1974 184 2095
Fragments arranged by size:
2095 1974 204 184
MscI TGG'CCA
Cuts at: 584 2218 584
Size: 1634 2823
MslI CAynn'nnrTG
Cuts at: 373 1118 1405 2446 2573 373
Size: 745 287 1041 127 2257
Fragments arranged by size:
2257 1041 745 287 127
MspA1I CmG'CkG
Cuts at: 1029 1568 1882 2242 3026 3271 1029
Size: 539 314 360 784 245 2215
Fragments arranged by size:
2215 784 539 360 314 245
NaeI GCC'GGC
Cuts at: 2638 2638
Size: 4457
NarI GG'CG_CC
Cuts at: 1078 1888 2136 1078
Size: 810 248 3399
Fragments arranged by size:
3399 810 248
NcoI C'CATG_G
Cuts at: 140 140
Size: 4457
NgoMIV G'CCGG_C
Cuts at: 2636 2636
Size: 4457
NheI G'CTAG_C
Cuts at: 117 117
Size: 4457
NspI r_CATG'y
Cuts at: 883 1510 2010 3613 883
Size: 627 500 1603 1727
Fragments arranged by size:
1727 1603 627 500
PciI A'CATG_T
Cuts at: 879 3609 879
Size: 2730 1727
PflMI CCAn_nnn'nTGG
Cuts at: 113 156 184 113
Size: 43 28 4386
Fragments arranged by size:
4386 43 28
PmeI GTTT'AAAC
Cuts at: 1456 1456
Size: 4457
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2903 2935 2903
Size: 32 4425
PsiI TTA'TAA
Cuts at: 758 758
Size: 4457
PspOMI G'GGCC_C
Cuts at: 1444 1444
Size: 4457
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 440 472 587 619 440
Size: 32 115 32 4278
Fragments arranged by size:
4278 115 32 32
PstI C_TGCA'G
Cuts at: 416 1226 1504 2189 416
Size: 810 278 685 2684
Fragments arranged by size:
2684 810 685 278
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4457
PvuII CAG'CTG
Cuts at: 1029 1882 2242 1029
Size: 853 360 3244
Fragments arranged by size:
3244 853 360
RsrII CG'GwC_CG
Cuts at: 2652 2652
Size: 4457
SacI G_AGCT'C
Cuts at: 1471 1471
Size: 4457
SalI G'TCGA_C
Cuts at: 1494 1494
Size: 4457
SapI GCTCTTCn'nnn_
Cuts at: 2480 2690 2480
Size: 210 4247
SbfI CC_TGCA'GG
Cuts at: 1504 1504
Size: 4457
ScaI AGT'ACT
Cuts at: 3974 3974
Size: 4457
SexAI A'CCwGG_T
Cuts at: 149 149
Size: 4457
SfcI C'TryA_G
Cuts at: 33 278 412 1040 1222 1500 2185 3153 3344
Size: 245 134 628 182 278 685 968 191
Cuts at: 3344 33
Size: 1146
Fragments arranged by size:
1146 968 685 628 278 245 191 182
134
SfoI GGC'GCC
Cuts at: 1079 1889 2137 1079
Size: 810 248 3399
Fragments arranged by size:
3399 810 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4457
SmaI CCC'GGG
Cuts at: 1479 1479
Size: 4457
SmlI C'TyrA_G
Cuts at: 1612 2527 3241 3503 1612
Size: 915 714 262 2566
Fragments arranged by size:
2566 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4457
SphI G_CATG'C
Cuts at: 1510 2010 1510
Size: 500 3957
SspI AAT'ATT
Cuts at: 680 680
Size: 4457
StuI AGG'CCT
Cuts at: 917 917
Size: 4457
StyI C'CwwG_G
Cuts at: 123 140 885 1591 1945 123
Size: 17 745 706 354 2635
Fragments arranged by size:
2635 745 706 354 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1395 2040 2173 2632 2841 1395
Size: 645 133 459 209 3011
Fragments arranged by size:
3011 645 459 209 133
TatI w'GTAC_w
Cuts at: 826 1284 3972 826
Size: 458 2688 1311
Fragments arranged by size:
2688 1311 458
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3974 4006 3974
Size: 32 4425
Tth111I GACn'n_nGTC
Cuts at: 2254 2254
Size: 4457
XbaI T'CTAG_A
Cuts at: 1488 1488
Size: 4457
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1952 1952
Size: 4457
XmaI C'CCGG_G
Cuts at: 1477 1477
Size: 4457
XmnI GAAnn'nnTTC
Cuts at: 1113 1113
Size: 4457
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI
PflMI PmeI PpiI PsiI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AvrII BaeI BbvCI BfrBI BglI BsiWI
BsmI BspEI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoNI FseI FspAI HindIII HpaI MfeI NdeI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PspXI SacII SanDI SfiI SgrAI SnaBI SrfI SwaI
XhoI ZraI