Restriction map for
pF1KB5029
(Circular) (Six-base) MAPSORT of: pF1KB5029.seq Check: 7313 from: 1 to: 4757
pF1KB5029 4757 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1181 1181
Size: 4757
AccI GT'mk_AC
Cuts at: 1664 1795 1664
Size: 131 4626
Acc65I G'GTAC_C
Cuts at: 1469 1773 1469
Size: 304 4453
AclI AA'CG_TT
Cuts at: 4597 4597
Size: 4757
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 572 1402 1671 2582 3014 3761 4029 572
Size: 830 269 911 432 747 268 1300
Fragments arranged by size:
1300 911 830 747 432 269 268
AflIII A'CryG_T
Cuts at: 446 2109 3213 4190 446
Size: 1663 1104 977 1013
Fragments arranged by size:
1663 1104 1013 977
AgeI A'CCGG_T
Cuts at: 3145 3145
Size: 4757
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 529 529
Size: 4757
AlwNI CAG_nnn'CTG
Cuts at: 471 1316 1344 2062 3629 471
Size: 845 28 718 1567 1599
Fragments arranged by size:
1599 1567 845 718 28
ApaI G_GGCC'C
Cuts at: 702 702
Size: 4757
ApaLI G'TGCA_C
Cuts at: 3527 3527
Size: 4757
ApoI r'AATT_y
Cuts at: 1761 4110 4366 1761
Size: 2349 256 2152
Fragments arranged by size:
2349 2152 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4757
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4757
AvaI C'yCGr_G
Cuts at: 172 578 1777 172
Size: 406 1199 3152
Fragments arranged by size:
3152 1199 406
AvrII C'CTAG_G
Cuts at: 1503 1503
Size: 4757
BamHI G'GATC_C
Cuts at: 1782 1782
Size: 4757
BanI G'GyrC_C
Cuts at: 1469 1507 1681 1773 2187 2435 2470 1469
Size: 38 174 92 414 248 35 3756
Fragments arranged by size:
3756 414 248 174 92 38 35
BanII G_rGCy'C
Cuts at: 702 1001 1771 2801 702
Size: 299 770 1030 2658
Fragments arranged by size:
2658 1030 770 299
BbeI G_GCGC'C
Cuts at: 2191 2439 2191
Size: 248 4509
BbsI GAAGACnn'nnnn_
Cuts at: 382 382
Size: 4757
BciVI GTATCCnnnnn_n'
Cuts at: 351 2655 3416 4757 351
Size: 2304 761 1341 351
Fragments arranged by size:
2304 1341 761 351
BclI T'GATC_A
Cuts at: 2277 2277
Size: 4757
BglI GCCn_nnn'nGGC
Cuts at: 862 1051 862
Size: 189 4568
BglII A'GATC_T
Cuts at: 2272 4309 2272
Size: 2037 2720
BlpI GC'TnA_GC
Cuts at: 1869 1869
Size: 4757
Bme1580I G_kGCm'C
Cuts at: 702 2382 2475 3531 702
Size: 1680 93 1056 1928
Fragments arranged by size:
1928 1680 1056 93
BmgBI CAC'GTC
Cuts at: 1497 1497
Size: 4757
BmrI ACTGGGnnnn_n'
Cuts at: 1969 2050 2385 4529 1969
Size: 81 335 2144 2197
Fragments arranged by size:
2197 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3124 3124
Size: 4757
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1274 1630 1274
Size: 356 4401
Bpu10I CC'TnA_GC
Cuts at: 1552 1552
Size: 4757
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 989 1110 1432 1618 1933 2812 3304 3602 989
Size: 121 322 186 315 879 492 298 2144
Fragments arranged by size:
2144 879 492 322 315 298 186 121
BsaI GGTCTCn'nnnn_
Cuts at: 4461 4461
Size: 4757
BsaAI yAC'GTr
Cuts at: 1476 2110 1476
Size: 634 4123
BsaBI GATnn'nnATC
Cuts at: 6 2271 4308 6
Size: 2265 2037 455
Fragments arranged by size:
2265 2037 455
BsaHI Gr'CG_yC
Cuts at: 1244 1335 2188 2436 3138 4634 1244
Size: 91 853 248 702 1496 1367
Fragments arranged by size:
1496 1367 853 702 248 91
BsaWI w'CCGG_w
Cuts at: 1946 2467 3145 3419 3566 3977 1946
Size: 521 678 274 147 411 2726
Fragments arranged by size:
2726 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1060 1090 1060
Size: 30 4727
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 276 343 756 1476 1627 1720 1729 1732 276
Size: 67 413 720 151 93 9 3 3301
Fragments arranged by size:
3301 720 413 151 93 67 9 3
BseYI C'CCAG_C
Cuts at: 152 1079 1511 2182 2940 3517 4013 152
Size: 927 432 671 758 577 496 896
Fragments arranged by size:
927 896 758 671 577 496 432
BsiEI CG_ry'CG
Cuts at: 104 2299 2345 3553 104
Size: 2195 46 1208 1308
Fragments arranged by size:
2195 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1238 1771 2549 2739 3531 1238
Size: 533 778 190 792 2464
Fragments arranged by size:
2464 792 778 533 190
BsmI GAATG_Cn'
Cuts at: 506 1569 506
Size: 1063 3694
Bsp1286I G_dGCh'C
Cuts at: 702 1001 1238 1771 2382 2475 2549 2739 2801
Size: 299 237 533 611 93 74 190 62
Cuts at: 2801 3531 702
Size: 730 1928
Fragments arranged by size:
1928 730 611 533 299 237 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3977 3977
Size: 4757
BspHI T'CATG_A
Cuts at: 119 1247 3933 119
Size: 1128 2686 943
Fragments arranged by size:
2686 1128 943
BspMI ACCTGCnnnn'nnnn_
Cuts at: 470 1181 1423 1641 1807 2021 2323 2704 470
Size: 711 242 218 166 214 302 381 2523
Fragments arranged by size:
2523 711 381 302 242 218 214 166
BsrBI CCG'CTC
Cuts at: 50 887 3049 3103 4587 4752 50
Size: 837 2162 54 1484 165 55
Fragments arranged by size:
2162 1484 837 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1367 2669 1367
Size: 1302 3455
BsrFI r'CCGG_y
Cuts at: 2755 2936 3145 2755
Size: 181 209 4367
Fragments arranged by size:
4367 209 181
BssHII G'CGCG_C
Cuts at: 364 364
Size: 4757
BssSI C'ACGA_G
Cuts at: 738 3028 3386 738
Size: 2290 358 2109
Fragments arranged by size:
2290 2109 358
BstAPI GCAn_nnn'nTGC
Cuts at: 471 2134 471
Size: 1663 3094
BstBI TT'CG_AA
Cuts at: 3118 3118
Size: 4757
BstXI CCAn_nnnn'nTGG
Cuts at: 922 922
Size: 4757
BstYI r'GATC_y
Cuts at: 1 467 1435 1782 2249 2272 2607 2853 3865
Size: 466 968 347 467 23 335 246 1012
Cuts at: 3865 3951 3963 4309 1
Size: 86 12 346 449
Fragments arranged by size:
1012 968 467 466 449 347 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 147 147
Size: 4757
BtgI C'CryG_G
Cuts at: 107 1046 1373 107
Size: 939 327 3491
Fragments arranged by size:
3491 939 327
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2707 2888 3051 2707
Size: 181 163 4413
Fragments arranged by size:
4413 181 163
BtsI GCAGTG_nn'
Cuts at: 754 1090 2000 754
Size: 336 910 3511
Fragments arranged by size:
3511 910 336
DraI TTT'AAA
Cuts at: 1756 1756
Size: 4757
DrdI GACnn_nn'nnGTC
Cuts at: 805 1222 2463 3321 805
Size: 417 1241 858 2241
Fragments arranged by size:
2241 1241 858 417
EaeI y'GGCC_r
Cuts at: 863 1043 2342 2516 2907 2934 4279 863
Size: 180 1299 174 391 27 1345 1341
Fragments arranged by size:
1345 1341 1299 391 180 174 27
EagI C'GGCC_G
Cuts at: 2342 2342
Size: 4757
EarI CTCTTCn'nnn_
Cuts at: 591 940 1706 1721 1733 2780 2990 591
Size: 349 766 15 12 1047 210 2358
Fragments arranged by size:
2358 1047 766 349 210 15 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2965 3275 3421 4567 2965
Size: 310 146 1146 3155
Fragments arranged by size:
3155 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1769 1769
Size: 4757
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 572 1274 1402 1630 1671 2582 3014 3761 4029
Size: 702 128 228 41 911 432 747 268
Cuts at: 4029 572
Size: 1300
Fragments arranged by size:
1300 911 747 702 432 268 228 128
41
EcoO109I rG'GnC_Cy
Cuts at: 157 698 699 1638 1896 157
Size: 541 1 939 258 3018
Fragments arranged by size:
3018 939 541 258 1
EcoRI G'AATT_C
Cuts at: 1761 1761
Size: 4757
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 792 2776 792
Size: 1984 2773
FspI TGC'GCA
Cuts at: 2538 2538
Size: 4757
HaeII r_GCGC'y
Cuts at: 2191 2439 3461 4440 2191
Size: 248 1022 979 2508
Fragments arranged by size:
2508 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 130 162 550 582 1349 1381 2599 2629 2631
Size: 32 388 32 767 32 1218 30 2
Cuts at: 2631 2661 130
Size: 30 2226
Fragments arranged by size:
2226 1218 767 388 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1329 1796 1329
Size: 467 4290
HindIII A'AGCT_T
Cuts at: 1198 1450 1198
Size: 252 4505
KasI G'GCGC_C
Cuts at: 2187 2435 2187
Size: 248 4509
KpnI G_GTAC'C
Cuts at: 1473 1777 1473
Size: 304 4453
MluI A'CGCG_T
Cuts at: 4190 4190
Size: 4757
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 421 3428 3612 421
Size: 3007 184 1566
Fragments arranged by size:
3007 1566 184
MscI TGG'CCA
Cuts at: 865 1045 2518 865
Size: 180 1473 3104
Fragments arranged by size:
3104 1473 180
MslI CAynn'nnrTG
Cuts at: 871 1319 1600 2746 2873 871
Size: 448 281 1146 127 2755
Fragments arranged by size:
2755 1146 448 281 127
MspA1I CmG'CkG
Cuts at: 351 390 1868 2182 2542 3555 3800 351
Size: 39 1478 314 360 1013 245 1308
Fragments arranged by size:
1478 1308 1013 360 314 245 39
NaeI GCC'GGC
Cuts at: 2938 2938
Size: 4757
NarI GG'CG_CC
Cuts at: 2188 2436 2188
Size: 248 4509
NcoI C'CATG_G
Cuts at: 107 1046 107
Size: 939 3818
NgoMIV G'CCGG_C
Cuts at: 2936 2936
Size: 4757
NspI r_CATG'y
Cuts at: 450 1810 2310 3217 450
Size: 1360 500 907 1990
Fragments arranged by size:
1990 1360 907 500
PciI A'CATG_T
Cuts at: 446 3213 446
Size: 2767 1990
PflMI CCAn_nnn'nTGG
Cuts at: 354 354
Size: 4757
PfoI T'CCnGG_A
Cuts at: 134 134
Size: 4757
PmeI GTTT'AAAC
Cuts at: 1756 1756
Size: 4757
PmlI CAC'GTG
Cuts at: 1476 1476
Size: 4757
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 3896
Size: 32 4725
PpuMI rG'GwC_Cy
Cuts at: 157 1638 157
Size: 1481 3276
PshAI GACnn'nnGTC
Cuts at: 1334 1334
Size: 4757
PspOMI G'GGCC_C
Cuts at: 698 698
Size: 4757
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1378 1410 1378
Size: 32 4725
PstI C_TGCA'G
Cuts at: 467 518 1804 2489 467
Size: 51 1286 685 2735
Fragments arranged by size:
2735 1286 685 51
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4757
PvuII CAG'CTG
Cuts at: 351 2182 2542 351
Size: 1831 360 2566
Fragments arranged by size:
2566 1831 360
RsrII CG'GwC_CG
Cuts at: 2952 2952
Size: 4757
SacI G_AGCT'C
Cuts at: 1771 1771
Size: 4757
SalI G'TCGA_C
Cuts at: 1794 1794
Size: 4757
SanDI GG'GwC_CC
Cuts at: 157 157
Size: 4757
SapI GCTCTTCn'nnn_
Cuts at: 2780 2990 2780
Size: 210 4547
SbfI CC_TGCA'GG
Cuts at: 1804 1804
Size: 4757
ScaI AGT'ACT
Cuts at: 283 4274 283
Size: 3991 766
SfcI C'TryA_G
Cuts at: 33 463 514 1665 1800 2485 3478 3669 33
Size: 430 51 1151 135 685 993 191 1121
Fragments arranged by size:
1151 1121 993 685 430 191 135 51
SfoI GGC'GCC
Cuts at: 2189 2437 2189
Size: 248 4509
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4757
SmaI CCC'GGG
Cuts at: 1779 1779
Size: 4757
SmlI C'TyrA_G
Cuts at: 1004 1089 1447 1633 1912 2827 3319 3581 1004
Size: 85 358 186 279 915 492 262 2180
Fragments arranged by size:
2180 915 492 358 279 262 186 85
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4757
SphI G_CATG'C
Cuts at: 1810 2310 1810
Size: 500 4257
StyI C'CwwG_G
Cuts at: 107 1046 1503 1891 2245 107
Size: 939 457 388 354 2619
Fragments arranged by size:
2619 939 457 388 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1525 2340 2473 2932 3141 1525
Size: 815 133 459 209 3141
Fragments arranged by size:
3141 815 459 209 133
TatI w'GTAC_w
Cuts at: 281 4272 281
Size: 3991 766
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 116 148 673 705 1060 1092 1404 1436 4274
Size: 32 525 32 355 32 312 32 2838
Cuts at: 4274 4306 116
Size: 32 567
Fragments arranged by size:
2838 567 525 355 312 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 805 2554 805
Size: 1749 3008
XbaI T'CTAG_A
Cuts at: 1788 1788
Size: 4757
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 535 2252 535
Size: 1717 3040
XmaI C'CCGG_G
Cuts at: 1777 1777
Size: 4757
XmnI GAAnn'nnTTC
Cuts at: 506 523 506
Size: 17 4740
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI BaeI
BbvCI BcgI BfrBI BmtI BsgI BsiWI BsmBI BsrGI
BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PsiI PspXI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI