(Circular) (Six-base) MAPSORT of: pF1KB5030.seq Check: 3204 from: 1 to: 3965 pF1KB5030 3965 bp With 182 enzymes: SgfI * March 2, 2010 14:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 449 449 Size: 3965 AccI GT'mk_AC Cuts at: 302 1003 302 Size: 701 3264 Acc65I G'GTAC_C Cuts at: 981 981 Size: 3965 AclI AA'CG_TT Cuts at: 3805 3805 Size: 3965 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1790 2222 2969 3237 1790 Size: 432 747 268 2518 Fragments arranged by size: 2518 747 432 268 AflIII A'CryG_T Cuts at: 248 1317 2421 3398 248 Size: 1069 1104 977 815 Fragments arranged by size: 1104 1069 977 815 AgeI A'CCGG_T Cuts at: 2353 2353 Size: 3965 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 301 301 Size: 3965 AlwNI CAG_nnn'CTG Cuts at: 1270 2837 1270 Size: 1567 2398 ApaLI G'TGCA_C Cuts at: 2735 2735 Size: 3965 ApoI r'AATT_y Cuts at: 586 969 3318 3574 586 Size: 383 2349 256 977 Fragments arranged by size: 2349 977 383 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3965 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3965 AvaI C'yCGr_G Cuts at: 141 985 141 Size: 844 3121 BamHI G'GATC_C Cuts at: 990 990 Size: 3965 BanI G'GyrC_C Cuts at: 277 574 981 1395 1643 1678 277 Size: 297 407 414 248 35 2564 Fragments arranged by size: 2564 414 407 297 248 35 BanII G_rGCy'C Cuts at: 979 2009 979 Size: 1030 2935 BbeI G_GCGC'C Cuts at: 1399 1647 1399 Size: 248 3717 BbsI GAAGACnn'nnnn_ Cuts at: 704 704 Size: 3965 BbvCI CC'TCA_GC Cuts at: 827 827 Size: 3965 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 147 181 147 Size: 34 3931 BciVI GTATCCnnnnn_n' Cuts at: 1863 2624 3965 1863 Size: 761 1341 1863 Fragments arranged by size: 1863 1341 761 BclI T'GATC_A Cuts at: 1485 1485 Size: 3965 BfrBI ATG'CAT Cuts at: 705 705 Size: 3965 BglII A'GATC_T Cuts at: 1480 3517 1480 Size: 2037 1928 BlpI GC'TnA_GC Cuts at: 1077 1077 Size: 3965 Bme1580I G_kGCm'C Cuts at: 1590 1683 2739 1590 Size: 93 1056 2816 Fragments arranged by size: 2816 1056 93 BmgBI CAC'GTC Cuts at: 249 249 Size: 3965 BmrI ACTGGGnnnn_n' Cuts at: 1177 1258 1593 3737 1177 Size: 81 335 2144 1405 Fragments arranged by size: 2144 1405 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2332 2332 Size: 3965 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 394 394 Size: 3965 Bpu10I CC'TnA_GC Cuts at: 827 827 Size: 3965 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 673 1141 2020 2512 2810 673 Size: 468 879 492 298 1828 Fragments arranged by size: 1828 879 492 468 298 BsaI GGTCTCn'nnnn_ Cuts at: 121 3669 121 Size: 3548 417 BsaAI yAC'GTr Cuts at: 642 1318 642 Size: 676 3289 BsaBI GATnn'nnATC Cuts at: 6 349 1479 3516 6 Size: 343 1130 2037 455 Fragments arranged by size: 2037 1130 455 343 BsaHI Gr'CG_yC Cuts at: 1396 1644 2346 3842 1396 Size: 248 702 1496 1519 Fragments arranged by size: 1519 1496 702 248 BsaWI w'CCGG_w Cuts at: 1154 1675 2353 2627 2774 3185 1154 Size: 521 678 274 147 411 1934 Fragments arranged by size: 1934 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 229 229 Size: 3965 BseYI C'CCAG_C Cuts at: 133 241 615 770 1390 2148 2725 3221 133 Size: 108 374 155 620 758 577 496 877 Fragments arranged by size: 877 758 620 577 496 374 155 108 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 664 664 Size: 3965 BsiEI CG_ry'CG Cuts at: 104 1507 1553 2761 104 Size: 1403 46 1208 1308 Fragments arranged by size: 1403 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 641 662 794 979 1757 1947 2739 641 Size: 21 132 185 778 190 792 1867 Fragments arranged by size: 1867 792 778 190 185 132 21 BsmBI CGTCTCn'nnnn_ Cuts at: 888 888 Size: 3965 Bsp1286I G_dGCh'C Cuts at: 641 662 794 979 1590 1683 1757 1947 2009 Size: 21 132 185 611 93 74 190 62 Cuts at: 2009 2739 641 Size: 730 1867 Fragments arranged by size: 1867 730 611 190 185 132 93 74 62 21 BspEI T'CCGG_A Cuts at: 3185 3185 Size: 3965 BspHI T'CATG_A Cuts at: 3141 3141 Size: 3965 BspMI ACCTGCnnnn'nnnn_ Cuts at: 449 1015 1229 1531 1912 449 Size: 566 214 302 381 2502 Fragments arranged by size: 2502 566 381 302 214 BsrBI CCG'CTC Cuts at: 50 548 2257 2311 3795 3960 50 Size: 498 1709 54 1484 165 55 Fragments arranged by size: 1709 1484 498 165 55 54 BsrDI GCAATG_nn' Cuts at: 1877 1877 Size: 3965 BsrFI r'CCGG_y Cuts at: 1963 2144 2353 1963 Size: 181 209 3575 Fragments arranged by size: 3575 209 181 BssSI C'ACGA_G Cuts at: 2236 2594 2236 Size: 358 3607 BstAPI GCAn_nnn'nTGC Cuts at: 1342 1342 Size: 3965 BstBI TT'CG_AA Cuts at: 2326 2326 Size: 3965 BstXI CCAn_nnnn'nTGG Cuts at: 519 747 519 Size: 228 3737 BstYI r'GATC_y Cuts at: 1 516 990 1457 1480 1815 2061 3073 3159 Size: 515 474 467 23 335 246 1012 86 Cuts at: 3159 3171 3517 1 Size: 12 346 449 Fragments arranged by size: 1012 515 474 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 174 201 839 107 Size: 67 27 638 3233 Fragments arranged by size: 3233 638 67 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1915 2096 2259 1915 Size: 181 163 3621 Fragments arranged by size: 3621 181 163 BtsI GCAGTG_nn' Cuts at: 792 1208 792 Size: 416 3549 DraI TTT'AAA Cuts at: 964 964 Size: 3965 DrdI GACnn_nn'nnGTC Cuts at: 487 1671 2529 487 Size: 1184 858 1923 Fragments arranged by size: 1923 1184 858 EaeI y'GGCC_r Cuts at: 737 1550 1724 2115 2142 3487 737 Size: 813 174 391 27 1345 1215 Fragments arranged by size: 1345 1215 813 391 174 27 EagI C'GGCC_G Cuts at: 1550 1550 Size: 3965 EarI CTCTTCn'nnn_ Cuts at: 846 1988 2198 846 Size: 1142 210 2613 Fragments arranged by size: 2613 1142 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 127 2173 2483 2629 3775 127 Size: 2046 310 146 1146 317 Fragments arranged by size: 2046 1146 317 310 146 EcoICRI GAG'CTC Cuts at: 977 977 Size: 3965 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 394 1790 2222 2969 3237 394 Size: 1396 432 747 268 1122 Fragments arranged by size: 1396 1122 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 711 831 1104 711 Size: 120 273 3572 Fragments arranged by size: 3572 273 120 EcoRI G'AATT_C Cuts at: 586 969 586 Size: 383 3582 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 891 921 1984 891 Size: 30 1063 2872 Fragments arranged by size: 2872 1063 30 FspI TGC'GCA Cuts at: 1746 1746 Size: 3965 HaeII r_GCGC'y Cuts at: 323 1399 1647 2669 3648 323 Size: 1076 248 1022 979 640 Fragments arranged by size: 1076 1022 979 640 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1807 1837 1839 1869 1807 Size: 30 2 30 3903 Fragments arranged by size: 3903 30 30 2 HincII GTy'rAC Cuts at: 1004 1004 Size: 3965 KasI G'GCGC_C Cuts at: 1395 1643 1395 Size: 248 3717 KpnI G_GTAC'C Cuts at: 985 985 Size: 3965 MluI A'CGCG_T Cuts at: 3398 3398 Size: 3965 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2636 2820 2636 Size: 184 3781 MscI TGG'CCA Cuts at: 739 1726 739 Size: 987 2978 MslI CAynn'nnrTG Cuts at: 496 1954 2081 496 Size: 1458 127 2380 Fragments arranged by size: 2380 1458 127 MspA1I CmG'CkG Cuts at: 203 735 774 804 1076 1390 1750 2763 3008 Size: 532 39 30 272 314 360 1013 245 Cuts at: 3008 203 Size: 1160 Fragments arranged by size: 1160 1013 532 360 314 272 245 39 30 NaeI GCC'GGC Cuts at: 2146 2146 Size: 3965 NarI GG'CG_CC Cuts at: 1396 1644 1396 Size: 248 3717 NcoI C'CATG_G Cuts at: 107 174 107 Size: 67 3898 NgoMIV G'CCGG_C Cuts at: 2144 2144 Size: 3965 NsiI A_TGCA'T Cuts at: 707 707 Size: 3965 NspI r_CATG'y Cuts at: 705 1018 1518 2425 705 Size: 313 500 907 2245 Fragments arranged by size: 2245 907 500 313 PciI A'CATG_T Cuts at: 2421 2421 Size: 3965 PflMI CCAn_nnn'nTGG Cuts at: 804 807 804 Size: 3 3962 PfoI T'CCnGG_A Cuts at: 371 371 Size: 3965 PmeI GTTT'AAAC Cuts at: 964 964 Size: 3965 PmlI CAC'GTG Cuts at: 642 642 Size: 3965 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3104 3136 3104 Size: 32 3933 PpuMI rG'GwC_Cy Cuts at: 711 711 Size: 3965 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 154 186 154 Size: 32 3933 PstI C_TGCA'G Cuts at: 293 1012 1697 293 Size: 719 685 2561 Fragments arranged by size: 2561 719 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 3965 PvuII CAG'CTG Cuts at: 735 774 804 1390 1750 735 Size: 39 30 586 360 2950 Fragments arranged by size: 2950 586 360 39 30 RsrII CG'GwC_CG Cuts at: 2160 2160 Size: 3965 SacI G_AGCT'C Cuts at: 979 979 Size: 3965 SacII CC_GC'GG Cuts at: 204 204 Size: 3965 SalI G'TCGA_C Cuts at: 1002 1002 Size: 3965 SapI GCTCTTCn'nnn_ Cuts at: 1988 2198 1988 Size: 210 3755 SbfI CC_TGCA'GG Cuts at: 1012 1012 Size: 3965 ScaI AGT'ACT Cuts at: 163 3482 163 Size: 3319 646 SexAI A'CCwGG_T Cuts at: 713 713 Size: 3965 SfcI C'TryA_G Cuts at: 33 289 1008 1693 2686 2877 33 Size: 256 719 685 993 191 1121 Fragments arranged by size: 1121 993 719 685 256 191 SfoI GGC'GCC Cuts at: 1397 1645 1397 Size: 248 3717 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3965 SmaI CCC'GGG Cuts at: 143 987 143 Size: 844 3121 SmlI C'TyrA_G Cuts at: 688 1120 2035 2527 2789 688 Size: 432 915 492 262 1864 Fragments arranged by size: 1864 915 492 432 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3965 SphI G_CATG'C Cuts at: 1018 1518 1018 Size: 500 3465 StyI C'CwwG_G Cuts at: 107 174 485 512 1099 1453 107 Size: 67 311 27 587 354 2619 Fragments arranged by size: 2619 587 354 311 67 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1548 1681 2140 2349 1548 Size: 133 459 209 3164 Fragments arranged by size: 3164 459 209 133 TatI w'GTAC_w Cuts at: 161 3480 161 Size: 3319 646 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3482 3514 3482 Size: 32 3933 Tth111I GACn'n_nGTC Cuts at: 1762 1762 Size: 3965 XbaI T'CTAG_A Cuts at: 996 996 Size: 3965 XcmI CCAnnnn_n'nnnnTGG Cuts at: 519 1460 519 Size: 941 3024 XmaI C'CCGG_G Cuts at: 141 985 141 Size: 844 3121 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AhdI AleI AloI ApaI AscI AvrII BaeI BglI BmtI BsaXI BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI PacI PasI PshAI PsiI PspOMI PspXI SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI