Restriction map for
pF1KB5030
(Circular) (Six-base) MAPSORT of: pF1KB5030.seq Check: 3204 from: 1 to: 3965
pF1KB5030 3965 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 449 449
Size: 3965
AccI GT'mk_AC
Cuts at: 302 1003 302
Size: 701 3264
Acc65I G'GTAC_C
Cuts at: 981 981
Size: 3965
AclI AA'CG_TT
Cuts at: 3805 3805
Size: 3965
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1790 2222 2969 3237 1790
Size: 432 747 268 2518
Fragments arranged by size:
2518 747 432 268
AflIII A'CryG_T
Cuts at: 248 1317 2421 3398 248
Size: 1069 1104 977 815
Fragments arranged by size:
1104 1069 977 815
AgeI A'CCGG_T
Cuts at: 2353 2353
Size: 3965
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 301 301
Size: 3965
AlwNI CAG_nnn'CTG
Cuts at: 1270 2837 1270
Size: 1567 2398
ApaLI G'TGCA_C
Cuts at: 2735 2735
Size: 3965
ApoI r'AATT_y
Cuts at: 586 969 3318 3574 586
Size: 383 2349 256 977
Fragments arranged by size:
2349 977 383 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3965
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3965
AvaI C'yCGr_G
Cuts at: 141 985 141
Size: 844 3121
BamHI G'GATC_C
Cuts at: 990 990
Size: 3965
BanI G'GyrC_C
Cuts at: 277 574 981 1395 1643 1678 277
Size: 297 407 414 248 35 2564
Fragments arranged by size:
2564 414 407 297 248 35
BanII G_rGCy'C
Cuts at: 979 2009 979
Size: 1030 2935
BbeI G_GCGC'C
Cuts at: 1399 1647 1399
Size: 248 3717
BbsI GAAGACnn'nnnn_
Cuts at: 704 704
Size: 3965
BbvCI CC'TCA_GC
Cuts at: 827 827
Size: 3965
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 147 181 147
Size: 34 3931
BciVI GTATCCnnnnn_n'
Cuts at: 1863 2624 3965 1863
Size: 761 1341 1863
Fragments arranged by size:
1863 1341 761
BclI T'GATC_A
Cuts at: 1485 1485
Size: 3965
BfrBI ATG'CAT
Cuts at: 705 705
Size: 3965
BglII A'GATC_T
Cuts at: 1480 3517 1480
Size: 2037 1928
BlpI GC'TnA_GC
Cuts at: 1077 1077
Size: 3965
Bme1580I G_kGCm'C
Cuts at: 1590 1683 2739 1590
Size: 93 1056 2816
Fragments arranged by size:
2816 1056 93
BmgBI CAC'GTC
Cuts at: 249 249
Size: 3965
BmrI ACTGGGnnnn_n'
Cuts at: 1177 1258 1593 3737 1177
Size: 81 335 2144 1405
Fragments arranged by size:
2144 1405 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2332 2332
Size: 3965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 394
Size: 3965
Bpu10I CC'TnA_GC
Cuts at: 827 827
Size: 3965
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 1141 2020 2512 2810 673
Size: 468 879 492 298 1828
Fragments arranged by size:
1828 879 492 468 298
BsaI GGTCTCn'nnnn_
Cuts at: 121 3669 121
Size: 3548 417
BsaAI yAC'GTr
Cuts at: 642 1318 642
Size: 676 3289
BsaBI GATnn'nnATC
Cuts at: 6 349 1479 3516 6
Size: 343 1130 2037 455
Fragments arranged by size:
2037 1130 455 343
BsaHI Gr'CG_yC
Cuts at: 1396 1644 2346 3842 1396
Size: 248 702 1496 1519
Fragments arranged by size:
1519 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1154 1675 2353 2627 2774 3185 1154
Size: 521 678 274 147 411 1934
Fragments arranged by size:
1934 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 229 229
Size: 3965
BseYI C'CCAG_C
Cuts at: 133 241 615 770 1390 2148 2725 3221 133
Size: 108 374 155 620 758 577 496 877
Fragments arranged by size:
877 758 620 577 496 374 155 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 664
Size: 3965
BsiEI CG_ry'CG
Cuts at: 104 1507 1553 2761 104
Size: 1403 46 1208 1308
Fragments arranged by size:
1403 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 641 662 794 979 1757 1947 2739 641
Size: 21 132 185 778 190 792 1867
Fragments arranged by size:
1867 792 778 190 185 132 21
BsmBI CGTCTCn'nnnn_
Cuts at: 888 888
Size: 3965
Bsp1286I G_dGCh'C
Cuts at: 641 662 794 979 1590 1683 1757 1947 2009
Size: 21 132 185 611 93 74 190 62
Cuts at: 2009 2739 641
Size: 730 1867
Fragments arranged by size:
1867 730 611 190 185 132 93 74
62 21
BspEI T'CCGG_A
Cuts at: 3185 3185
Size: 3965
BspHI T'CATG_A
Cuts at: 3141 3141
Size: 3965
BspMI ACCTGCnnnn'nnnn_
Cuts at: 449 1015 1229 1531 1912 449
Size: 566 214 302 381 2502
Fragments arranged by size:
2502 566 381 302 214
BsrBI CCG'CTC
Cuts at: 50 548 2257 2311 3795 3960 50
Size: 498 1709 54 1484 165 55
Fragments arranged by size:
1709 1484 498 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1877 1877
Size: 3965
BsrFI r'CCGG_y
Cuts at: 1963 2144 2353 1963
Size: 181 209 3575
Fragments arranged by size:
3575 209 181
BssSI C'ACGA_G
Cuts at: 2236 2594 2236
Size: 358 3607
BstAPI GCAn_nnn'nTGC
Cuts at: 1342 1342
Size: 3965
BstBI TT'CG_AA
Cuts at: 2326 2326
Size: 3965
BstXI CCAn_nnnn'nTGG
Cuts at: 519 747 519
Size: 228 3737
BstYI r'GATC_y
Cuts at: 1 516 990 1457 1480 1815 2061 3073 3159
Size: 515 474 467 23 335 246 1012 86
Cuts at: 3159 3171 3517 1
Size: 12 346 449
Fragments arranged by size:
1012 515 474 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 174 201 839 107
Size: 67 27 638 3233
Fragments arranged by size:
3233 638 67 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1915 2096 2259 1915
Size: 181 163 3621
Fragments arranged by size:
3621 181 163
BtsI GCAGTG_nn'
Cuts at: 792 1208 792
Size: 416 3549
DraI TTT'AAA
Cuts at: 964 964
Size: 3965
DrdI GACnn_nn'nnGTC
Cuts at: 487 1671 2529 487
Size: 1184 858 1923
Fragments arranged by size:
1923 1184 858
EaeI y'GGCC_r
Cuts at: 737 1550 1724 2115 2142 3487 737
Size: 813 174 391 27 1345 1215
Fragments arranged by size:
1345 1215 813 391 174 27
EagI C'GGCC_G
Cuts at: 1550 1550
Size: 3965
EarI CTCTTCn'nnn_
Cuts at: 846 1988 2198 846
Size: 1142 210 2613
Fragments arranged by size:
2613 1142 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 2173 2483 2629 3775 127
Size: 2046 310 146 1146 317
Fragments arranged by size:
2046 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 977 977
Size: 3965
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 1790 2222 2969 3237 394
Size: 1396 432 747 268 1122
Fragments arranged by size:
1396 1122 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 711 831 1104 711
Size: 120 273 3572
Fragments arranged by size:
3572 273 120
EcoRI G'AATT_C
Cuts at: 586 969 586
Size: 383 3582
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 891 921 1984 891
Size: 30 1063 2872
Fragments arranged by size:
2872 1063 30
FspI TGC'GCA
Cuts at: 1746 1746
Size: 3965
HaeII r_GCGC'y
Cuts at: 323 1399 1647 2669 3648 323
Size: 1076 248 1022 979 640
Fragments arranged by size:
1076 1022 979 640 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1807 1837 1839 1869 1807
Size: 30 2 30 3903
Fragments arranged by size:
3903 30 30 2
HincII GTy'rAC
Cuts at: 1004 1004
Size: 3965
KasI G'GCGC_C
Cuts at: 1395 1643 1395
Size: 248 3717
KpnI G_GTAC'C
Cuts at: 985 985
Size: 3965
MluI A'CGCG_T
Cuts at: 3398 3398
Size: 3965
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2636 2820 2636
Size: 184 3781
MscI TGG'CCA
Cuts at: 739 1726 739
Size: 987 2978
MslI CAynn'nnrTG
Cuts at: 496 1954 2081 496
Size: 1458 127 2380
Fragments arranged by size:
2380 1458 127
MspA1I CmG'CkG
Cuts at: 203 735 774 804 1076 1390 1750 2763 3008
Size: 532 39 30 272 314 360 1013 245
Cuts at: 3008 203
Size: 1160
Fragments arranged by size:
1160 1013 532 360 314 272 245 39
30
NaeI GCC'GGC
Cuts at: 2146 2146
Size: 3965
NarI GG'CG_CC
Cuts at: 1396 1644 1396
Size: 248 3717
NcoI C'CATG_G
Cuts at: 107 174 107
Size: 67 3898
NgoMIV G'CCGG_C
Cuts at: 2144 2144
Size: 3965
NsiI A_TGCA'T
Cuts at: 707 707
Size: 3965
NspI r_CATG'y
Cuts at: 705 1018 1518 2425 705
Size: 313 500 907 2245
Fragments arranged by size:
2245 907 500 313
PciI A'CATG_T
Cuts at: 2421 2421
Size: 3965
PflMI CCAn_nnn'nTGG
Cuts at: 804 807 804
Size: 3 3962
PfoI T'CCnGG_A
Cuts at: 371 371
Size: 3965
PmeI GTTT'AAAC
Cuts at: 964 964
Size: 3965
PmlI CAC'GTG
Cuts at: 642 642
Size: 3965
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3104 3136 3104
Size: 32 3933
PpuMI rG'GwC_Cy
Cuts at: 711 711
Size: 3965
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 154 186 154
Size: 32 3933
PstI C_TGCA'G
Cuts at: 293 1012 1697 293
Size: 719 685 2561
Fragments arranged by size:
2561 719 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3965
PvuII CAG'CTG
Cuts at: 735 774 804 1390 1750 735
Size: 39 30 586 360 2950
Fragments arranged by size:
2950 586 360 39 30
RsrII CG'GwC_CG
Cuts at: 2160 2160
Size: 3965
SacI G_AGCT'C
Cuts at: 979 979
Size: 3965
SacII CC_GC'GG
Cuts at: 204 204
Size: 3965
SalI G'TCGA_C
Cuts at: 1002 1002
Size: 3965
SapI GCTCTTCn'nnn_
Cuts at: 1988 2198 1988
Size: 210 3755
SbfI CC_TGCA'GG
Cuts at: 1012 1012
Size: 3965
ScaI AGT'ACT
Cuts at: 163 3482 163
Size: 3319 646
SexAI A'CCwGG_T
Cuts at: 713 713
Size: 3965
SfcI C'TryA_G
Cuts at: 33 289 1008 1693 2686 2877 33
Size: 256 719 685 993 191 1121
Fragments arranged by size:
1121 993 719 685 256 191
SfoI GGC'GCC
Cuts at: 1397 1645 1397
Size: 248 3717
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3965
SmaI CCC'GGG
Cuts at: 143 987 143
Size: 844 3121
SmlI C'TyrA_G
Cuts at: 688 1120 2035 2527 2789 688
Size: 432 915 492 262 1864
Fragments arranged by size:
1864 915 492 432 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3965
SphI G_CATG'C
Cuts at: 1018 1518 1018
Size: 500 3465
StyI C'CwwG_G
Cuts at: 107 174 485 512 1099 1453 107
Size: 67 311 27 587 354 2619
Fragments arranged by size:
2619 587 354 311 67 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1548 1681 2140 2349 1548
Size: 133 459 209 3164
Fragments arranged by size:
3164 459 209 133
TatI w'GTAC_w
Cuts at: 161 3480 161
Size: 3319 646
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3482 3514 3482
Size: 32 3933
Tth111I GACn'n_nGTC
Cuts at: 1762 1762
Size: 3965
XbaI T'CTAG_A
Cuts at: 996 996
Size: 3965
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 519 1460 519
Size: 941 3024
XmaI C'CCGG_G
Cuts at: 141 985 141
Size: 844 3121
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BglI BmtI BsaXI BsiWI BsmI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PasI PshAI PsiI PspOMI PspXI
SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI