Restriction map for
pF1KB5034
(Circular) (Six-base) MAPSORT of: pF1KB5034.seq Check: 3352 from: 1 to: 4061
pF1KB5034 4061 bp
With 182 enzymes: SgfI *
February 15, 2010 17:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 468 468
Size: 4061
AccI GT'mk_AC
Cuts at: 140 1099 140
Size: 959 3102
Acc65I G'GTAC_C
Cuts at: 1077 1077
Size: 4061
AclI AA'CG_TT
Cuts at: 3901 3901
Size: 4061
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 628 1886 2318 3065 3333 238
Size: 390 1258 432 747 268 966
Fragments arranged by size:
1258 966 747 432 390 268
AflIII A'CryG_T
Cuts at: 1413 2517 3494 1413
Size: 1104 977 1980
Fragments arranged by size:
1980 1104 977
AgeI A'CCGG_T
Cuts at: 2449 2449
Size: 4061
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 847 879 847
Size: 32 4029
AlwNI CAG_nnn'CTG
Cuts at: 606 950 1366 2933 606
Size: 344 416 1567 1734
Fragments arranged by size:
1734 1567 416 344
ApaI G_GGCC'C
Cuts at: 171 171
Size: 4061
ApaLI G'TGCA_C
Cuts at: 2831 2831
Size: 4061
ApoI r'AATT_y
Cuts at: 1065 3414 3670 1065
Size: 2349 256 1456
Fragments arranged by size:
2349 1456 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4061
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
AvaI C'yCGr_G
Cuts at: 349 591 1081 349
Size: 242 490 3329
Fragments arranged by size:
3329 490 242
BamHI G'GATC_C
Cuts at: 518 1086 518
Size: 568 3493
BanI G'GyrC_C
Cuts at: 931 1077 1491 1739 1774 931
Size: 146 414 248 35 3218
Fragments arranged by size:
3218 414 248 146 35
BanII G_rGCy'C
Cuts at: 171 215 1075 2105 171
Size: 44 860 1030 2127
Fragments arranged by size:
2127 1030 860 44
BbeI G_GCGC'C
Cuts at: 1495 1743 1495
Size: 248 3813
BbsI GAAGACnn'nnnn_
Cuts at: 754 754
Size: 4061
BbvCI CC'TCA_GC
Cuts at: 979 979
Size: 4061
BciVI GTATCCnnnnn_n'
Cuts at: 1959 2720 4061 1959
Size: 761 1341 1959
Fragments arranged by size:
1959 1341 761
BclI T'GATC_A
Cuts at: 1581 1581
Size: 4061
BfrBI ATG'CAT
Cuts at: 565 565
Size: 4061
BglI GCCn_nnn'nGGC
Cuts at: 322 781 322
Size: 459 3602
BglII A'GATC_T
Cuts at: 1576 3613 1576
Size: 2037 2024
BlpI GC'TnA_GC
Cuts at: 1173 1173
Size: 4061
Bme1580I G_kGCm'C
Cuts at: 171 490 936 1686 1779 2835 171
Size: 319 446 750 93 1056 1397
Fragments arranged by size:
1397 1056 750 446 319 93
BmrI ACTGGGnnnn_n'
Cuts at: 255 477 654 1273 1354 1689 3833 255
Size: 222 177 619 81 335 2144 483
Fragments arranged by size:
2144 619 483 335 222 177 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 958 979 958
Size: 21 4040
Bpu10I CC'TnA_GC
Cuts at: 979 979
Size: 4061
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1237 2116 2608 2906 1237
Size: 879 492 298 2392
Fragments arranged by size:
2392 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 841 3765 841
Size: 2924 1137
BsaAI yAC'GTr
Cuts at: 510 1414 510
Size: 904 3157
BsaBI GATnn'nnATC
Cuts at: 6 1575 3612 6
Size: 1569 2037 455
Fragments arranged by size:
2037 1569 455
BsaHI Gr'CG_yC
Cuts at: 1492 1740 2442 3938 1492
Size: 248 702 1496 1615
Fragments arranged by size:
1615 1496 702 248
BsaWI w'CCGG_w
Cuts at: 419 1250 1771 2449 2723 2870 3281 419
Size: 831 521 678 274 147 411 1199
Fragments arranged by size:
1199 831 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 620 650 847 877 620
Size: 30 197 30 3804
Fragments arranged by size:
3804 197 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 367 487 784 367
Size: 120 297 3644
Fragments arranged by size:
3644 297 120
BseYI C'CCAG_C
Cuts at: 620 640 692 988 1486 2244 2821 3317 620
Size: 20 52 296 498 758 577 496 1364
Fragments arranged by size:
1364 758 577 498 496 296 52 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 502 400
Size: 102 3959
BsiEI CG_ry'CG
Cuts at: 104 246 1603 1649 2857 104
Size: 142 1357 46 1208 1308
Fragments arranged by size:
1357 1308 1208 142 46
BsiHKAI G_wGCw'C
Cuts at: 215 630 1075 1853 2043 2835 215
Size: 415 445 778 190 792 1441
Fragments arranged by size:
1441 792 778 445 415 190
Bsp1286I G_dGCh'C
Cuts at: 171 215 490 630 936 1075 1686 1779 1853
Size: 44 275 140 306 139 611 93 74
Cuts at: 1853 2043 2105 2835 171
Size: 190 62 730 1397
Fragments arranged by size:
1397 730 611 306 275 190 140 139
93 74 62 44
BspEI T'CCGG_A
Cuts at: 3281 3281
Size: 4061
BspHI T'CATG_A
Cuts at: 3237 3237
Size: 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 468 1111 1325 1627 2008 468
Size: 643 214 302 381 2521
Fragments arranged by size:
2521 643 381 302 214
BsrBI CCG'CTC
Cuts at: 50 516 2353 2407 3891 4056 50
Size: 466 1837 54 1484 165 55
Fragments arranged by size:
1837 1484 466 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1973 1973
Size: 4061
BsrFI r'CCGG_y
Cuts at: 826 2059 2240 2449 826
Size: 1233 181 209 2438
Fragments arranged by size:
2438 1233 209 181
BsrGI T'GTAC_A
Cuts at: 870 870
Size: 4061
BssSI C'ACGA_G
Cuts at: 2332 2690 2332
Size: 358 3703
BstAPI GCAn_nnn'nTGC
Cuts at: 1438 1438
Size: 4061
BstBI TT'CG_AA
Cuts at: 2422 2422
Size: 4061
BstXI CCAn_nnnn'nTGG
Cuts at: 444 444
Size: 4061
BstYI r'GATC_y
Cuts at: 1 518 585 1086 1553 1576 1911 2157 3169
Size: 517 67 501 467 23 335 246 1012
Cuts at: 3169 3255 3267 3613 1
Size: 86 12 346 449
Fragments arranged by size:
1012 517 501 467 449 346 335 246
86 67 23 12
BtgI C'CryG_G
Cuts at: 320 407 320
Size: 87 3974
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2011 2192 2355 2011
Size: 181 163 3717
Fragments arranged by size:
3717 181 163
BtsI GCAGTG_nn'
Cuts at: 490 1304 490
Size: 814 3247
DraI TTT'AAA
Cuts at: 1060 1060
Size: 4061
DrdI GACnn_nn'nnGTC
Cuts at: 1767 2625 1767
Size: 858 3203
EaeI y'GGCC_r
Cuts at: 314 839 1007 1646 1820 2211 2238 3583 314
Size: 525 168 639 174 391 27 1345 792
Fragments arranged by size:
1345 792 639 525 391 174 168 27
EagI C'GGCC_G
Cuts at: 1646 1646
Size: 4061
EarI CTCTTCn'nnn_
Cuts at: 2084 2294 2084
Size: 210 3851
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2269 2579 2725 3871 2269
Size: 310 146 1146 2459
Fragments arranged by size:
2459 1146 310 146
EcoICRI GAG'CTC
Cuts at: 213 1073 213
Size: 860 3201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 628 958 979 1886 2318 3065 3333 238
Size: 390 330 21 907 432 747 268 966
Fragments arranged by size:
966 907 747 432 390 330 268 21
EcoNI CCTnn'n_nnAGG
Cuts at: 175 352 175
Size: 177 3884
EcoO109I rG'GnC_Cy
Cuts at: 167 168 656 1200 167
Size: 1 488 544 3028
Fragments arranged by size:
3028 544 488 1
EcoRI G'AATT_C
Cuts at: 1065 1065
Size: 4061
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 294 2080 294
Size: 1786 2275
FspI TGC'GCA
Cuts at: 1842 1842
Size: 4061
HaeII r_GCGC'y
Cuts at: 433 1495 1743 2765 3744 433
Size: 1062 248 1022 979 750
Fragments arranged by size:
1062 1022 979 750 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1903 1933 1935 1965 1903
Size: 30 2 30 3999
Fragments arranged by size:
3999 30 30 2
HincII GTy'rAC
Cuts at: 1100 1100
Size: 4061
KasI G'GCGC_C
Cuts at: 1491 1739 1491
Size: 248 3813
KpnI G_GTAC'C
Cuts at: 1081 1081
Size: 4061
MluI A'CGCG_T
Cuts at: 3494 3494
Size: 4061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2732 2916 2732
Size: 184 3877
MscI TGG'CCA
Cuts at: 316 841 1822 316
Size: 525 981 2555
Fragments arranged by size:
2555 981 525
MslI CAynn'nnrTG
Cuts at: 2050 2177 2050
Size: 127 3934
MspA1I CmG'CkG
Cuts at: 115 950 1012 1172 1486 1846 2859 3104 115
Size: 835 62 160 314 360 1013 245 1072
Fragments arranged by size:
1072 1013 835 360 314 245 160 62
NaeI GCC'GGC
Cuts at: 828 2242 828
Size: 1414 2647
NarI GG'CG_CC
Cuts at: 1492 1740 1492
Size: 248 3813
NcoI C'CATG_G
Cuts at: 407 407
Size: 4061
NgoMIV G'CCGG_C
Cuts at: 826 2240 826
Size: 1414 2647
NsiI A_TGCA'T
Cuts at: 567 567
Size: 4061
NspI r_CATG'y
Cuts at: 801 894 1114 1614 2521 801
Size: 93 220 500 907 2341
Fragments arranged by size:
2341 907 500 220 93
PciI A'CATG_T
Cuts at: 2517 2517
Size: 4061
PmeI GTTT'AAAC
Cuts at: 1060 1060
Size: 4061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 847 879 3200 3232 847
Size: 32 2321 32 1676
Fragments arranged by size:
2321 1676 32 32
PpuMI rG'GwC_Cy
Cuts at: 656 656
Size: 4061
PspOMI G'GGCC_C
Cuts at: 167 167
Size: 4061
PspXI vC'TCGA_Gb
Cuts at: 349 349
Size: 4061
PstI C_TGCA'G
Cuts at: 1108 1793 1108
Size: 685 3376
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4061
PvuII CAG'CTG
Cuts at: 950 1486 1846 950
Size: 536 360 3165
Fragments arranged by size:
3165 536 360
RsrII CG'GwC_CG
Cuts at: 2256 2256
Size: 4061
SacI G_AGCT'C
Cuts at: 215 1075 215
Size: 860 3201
SalI G'TCGA_C
Cuts at: 1098 1098
Size: 4061
SanDI GG'GwC_CC
Cuts at: 656 656
Size: 4061
SapI GCTCTTCn'nnn_
Cuts at: 2084 2294 2084
Size: 210 3851
SbfI CC_TGCA'GG
Cuts at: 1108 1108
Size: 4061
ScaI AGT'ACT
Cuts at: 3578 3578
Size: 4061
SfcI C'TryA_G
Cuts at: 33 1104 1789 2782 2973 33
Size: 1071 685 993 191 1121
Fragments arranged by size:
1121 1071 993 685 191
SfoI GGC'GCC
Cuts at: 1493 1741 1493
Size: 248 3813
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
SmaI CCC'GGG
Cuts at: 1083 1083
Size: 4061
SmlI C'TyrA_G
Cuts at: 349 1216 2131 2623 2885 349
Size: 867 915 492 262 1525
Fragments arranged by size:
1525 915 867 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4061
SphI G_CATG'C
Cuts at: 1114 1614 1114
Size: 500 3561
StyI C'CwwG_G
Cuts at: 230 407 437 491 851 1195 1549 230
Size: 177 30 54 360 344 354 2742
Fragments arranged by size:
2742 360 354 344 177 54 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 274 1644 1777 2236 2445 274
Size: 1370 133 459 209 1890
Fragments arranged by size:
1890 1370 459 209 133
TatI w'GTAC_w
Cuts at: 398 870 3576 398
Size: 472 2706 883
Fragments arranged by size:
2706 883 472
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 620 652 3578 3610 620
Size: 32 2926 32 1071
Fragments arranged by size:
2926 1071 32 32
Tth111I GACn'n_nGTC
Cuts at: 1858 1858
Size: 4061
XbaI T'CTAG_A
Cuts at: 1092 1092
Size: 4061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 849 1556 849
Size: 707 3354
XhoI C'TCGA_G
Cuts at: 349 349
Size: 4061
XmaI C'CCGG_G
Cuts at: 1081 1081
Size: 4061
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PmeI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI AvrII
BaeI BcgI BmgBI BmtI BsiWI BsmI BsmBI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PshAI PsiI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XmnI ZraI