Restriction map for
pF1KB5035
(Circular) (Six-base) MAPSORT of: pF1KB5035.seq Check: 3384 from: 1 to: 3689
pF1KB5035 3689 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 727 727
Size: 3689
Acc65I G'GTAC_C
Cuts at: 705 705
Size: 3689
AclI AA'CG_TT
Cuts at: 3529 3529
Size: 3689
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 665 1514 1946 2693 2961 482
Size: 183 849 432 747 268 1210
Fragments arranged by size:
1210 849 747 432 268 183
AflIII A'CryG_T
Cuts at: 1041 2145 3122 1041
Size: 1104 977 1608
Fragments arranged by size:
1608 1104 977
AgeI A'CCGG_T
Cuts at: 2077 2077
Size: 3689
AlwNI CAG_nnn'CTG
Cuts at: 994 2561 994
Size: 1567 2122
ApaLI G'TGCA_C
Cuts at: 2459 2459
Size: 3689
ApoI r'AATT_y
Cuts at: 393 693 3042 3298 393
Size: 300 2349 256 784
Fragments arranged by size:
2349 784 300 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3689
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3689
AvaI C'yCGr_G
Cuts at: 388 709 388
Size: 321 3368
BamHI G'GATC_C
Cuts at: 714 714
Size: 3689
BanI G'GyrC_C
Cuts at: 705 1119 1367 1402 705
Size: 414 248 35 2992
Fragments arranged by size:
2992 414 248 35
BanII G_rGCy'C
Cuts at: 118 243 703 1733 118
Size: 125 460 1030 2074
Fragments arranged by size:
2074 1030 460 125
BbeI G_GCGC'C
Cuts at: 1123 1371 1123
Size: 248 3441
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3655
BciVI GTATCCnnnnn_n'
Cuts at: 1587 2348 3689 1587
Size: 761 1341 1587
Fragments arranged by size:
1587 1341 761
BclI T'GATC_A
Cuts at: 1209 1209
Size: 3689
BglII A'GATC_T
Cuts at: 205 1204 3241 205
Size: 999 2037 653
Fragments arranged by size:
2037 999 653
BlpI GC'TnA_GC
Cuts at: 801 801
Size: 3689
Bme1580I G_kGCm'C
Cuts at: 162 1314 1407 2463 162
Size: 1152 93 1056 1388
Fragments arranged by size:
1388 1152 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 901 982 1317 3461 901
Size: 81 335 2144 1129
Fragments arranged by size:
2144 1129 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 666 2056 666
Size: 1390 2299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 636 636
Size: 3689
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1744 2236 2534 865
Size: 879 492 298 2020
Fragments arranged by size:
2020 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3393 3393
Size: 3689
BsaAI yAC'GTr
Cuts at: 1042 1042
Size: 3689
BsaBI GATnn'nnATC
Cuts at: 6 1203 3240 6
Size: 1197 2037 455
Fragments arranged by size:
2037 1197 455
BsaHI Gr'CG_yC
Cuts at: 1120 1368 2070 3566 1120
Size: 248 702 1496 1243
Fragments arranged by size:
1496 1243 702 248
BsaWI w'CCGG_w
Cuts at: 878 1399 2077 2351 2498 2909 878
Size: 521 678 274 147 411 1658
Fragments arranged by size:
1658 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 538 538
Size: 3689
BseYI C'CCAG_C
Cuts at: 1114 1872 2449 2945 1114
Size: 758 577 496 1858
Fragments arranged by size:
1858 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 282 282
Size: 3689
BsiEI CG_ry'CG
Cuts at: 104 1231 1277 2485 104
Size: 1127 46 1208 1308
Fragments arranged by size:
1308 1208 1127 46
BsiHKAI G_wGCw'C
Cuts at: 243 703 1481 1671 2463 243
Size: 460 778 190 792 1469
Fragments arranged by size:
1469 792 778 460 190
BsmI GAATG_Cn'
Cuts at: 129 129
Size: 3689
Bsp1286I G_dGCh'C
Cuts at: 118 162 243 703 1314 1407 1481 1671 1733
Size: 44 81 460 611 93 74 190 62
Cuts at: 1733 2463 118
Size: 730 1344
Fragments arranged by size:
1344 730 611 460 190 93 81 74
62 44
BspEI T'CCGG_A
Cuts at: 2909 2909
Size: 3689
BspHI T'CATG_A
Cuts at: 2865 2865
Size: 3689
BspMI ACCTGCnnnn'nnnn_
Cuts at: 739 953 1255 1636 739
Size: 214 302 381 2792
Fragments arranged by size:
2792 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1981 2035 3519 3684 50
Size: 1931 54 1484 165 55
Fragments arranged by size:
1931 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1601 1601
Size: 3689
BsrFI r'CCGG_y
Cuts at: 1687 1868 2077 1687
Size: 181 209 3299
Fragments arranged by size:
3299 209 181
BssSI C'ACGA_G
Cuts at: 1960 2318 1960
Size: 358 3331
BstAPI GCAn_nnn'nTGC
Cuts at: 1066 1066
Size: 3689
BstBI TT'CG_AA
Cuts at: 2050 2050
Size: 3689
BstYI r'GATC_y
Cuts at: 1 205 714 1181 1204 1539 1785 2797 2883
Size: 204 509 467 23 335 246 1012 86
Cuts at: 2883 2895 3241 1
Size: 12 346 449
Fragments arranged by size:
1012 509 467 449 346 335 246 204
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 449 449
Size: 3689
BtgI C'CryG_G
Cuts at: 142 561 142
Size: 419 3270
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1639 1820 1983 1639
Size: 181 163 3345
Fragments arranged by size:
3345 181 163
BtsI GCAGTG_nn'
Cuts at: 194 932 194
Size: 738 2951
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 131 166 131
Size: 35 3654
DraI TTT'AAA
Cuts at: 419 688 419
Size: 269 3420
DrdI GACnn_nn'nnGTC
Cuts at: 1395 2253 1395
Size: 858 2831
EaeI y'GGCC_r
Cuts at: 1274 1448 1839 1866 3211 1274
Size: 174 391 27 1345 1752
Fragments arranged by size:
1752 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1274 1274
Size: 3689
EarI CTCTTCn'nnn_
Cuts at: 1712 1922 1712
Size: 210 3479
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1897 2207 2353 3499 1897
Size: 310 146 1146 2087
Fragments arranged by size:
2087 1146 310 146
EcoICRI GAG'CTC
Cuts at: 241 701 241
Size: 460 3229
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 636 665 1514 1946 2693 2961 482
Size: 154 29 849 432 747 268 1210
Fragments arranged by size:
1210 849 747 432 268 154 29
EcoO109I rG'GnC_Cy
Cuts at: 445 828 445
Size: 383 3306
EcoRI G'AATT_C
Cuts at: 693 693
Size: 3689
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1708 1708
Size: 3689
FspI TGC'GCA
Cuts at: 1470 1470
Size: 3689
HaeII r_GCGC'y
Cuts at: 1123 1371 2393 3372 1123
Size: 248 1022 979 1440
Fragments arranged by size:
1440 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1531 1561 1563 1593 1531
Size: 30 2 30 3627
Fragments arranged by size:
3627 30 30 2
HincII GTy'rAC
Cuts at: 728 728
Size: 3689
KasI G'GCGC_C
Cuts at: 1119 1367 1119
Size: 248 3441
KpnI G_GTAC'C
Cuts at: 709 709
Size: 3689
MluI A'CGCG_T
Cuts at: 3122 3122
Size: 3689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2360 2544 2360
Size: 184 3505
MscI TGG'CCA
Cuts at: 1450 1450
Size: 3689
MslI CAynn'nnrTG
Cuts at: 1678 1805 1678
Size: 127 3562
MspA1I CmG'CkG
Cuts at: 176 438 800 1114 1474 2487 2732 176
Size: 262 362 314 360 1013 245 1133
Fragments arranged by size:
1133 1013 362 360 314 262 245
NaeI GCC'GGC
Cuts at: 1870 1870
Size: 3689
NarI GG'CG_CC
Cuts at: 1120 1368 1120
Size: 248 3441
NcoI C'CATG_G
Cuts at: 561 561
Size: 3689
NgoMIV G'CCGG_C
Cuts at: 1868 1868
Size: 3689
NspI r_CATG'y
Cuts at: 742 1242 2149 742
Size: 500 907 2282
Fragments arranged by size:
2282 907 500
PciI A'CATG_T
Cuts at: 2145 2145
Size: 3689
PmeI GTTT'AAAC
Cuts at: 688 688
Size: 3689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2828 2860 2828
Size: 32 3657
PpuMI rG'GwC_Cy
Cuts at: 445 445
Size: 3689
PstI C_TGCA'G
Cuts at: 128 443 736 1421 128
Size: 315 293 685 2396
Fragments arranged by size:
2396 685 315 293
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3689
PvuII CAG'CTG
Cuts at: 438 1114 1474 438
Size: 676 360 2653
Fragments arranged by size:
2653 676 360
RsrII CG'GwC_CG
Cuts at: 1884 1884
Size: 3689
SacI G_AGCT'C
Cuts at: 243 703 243
Size: 460 3229
SalI G'TCGA_C
Cuts at: 726 726
Size: 3689
SapI GCTCTTCn'nnn_
Cuts at: 1712 1922 1712
Size: 210 3479
SbfI CC_TGCA'GG
Cuts at: 736 736
Size: 3689
ScaI AGT'ACT
Cuts at: 3206 3206
Size: 3689
SfcI C'TryA_G
Cuts at: 33 124 439 732 1417 2410 2601 33
Size: 91 315 293 685 993 191 1121
Fragments arranged by size:
1121 993 685 315 293 191 91
SfoI GGC'GCC
Cuts at: 1121 1369 1121
Size: 248 3441
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3689
SmaI CCC'GGG
Cuts at: 711 711
Size: 3689
SmlI C'TyrA_G
Cuts at: 388 844 1759 2251 2513 388
Size: 456 915 492 262 1564
Fragments arranged by size:
1564 915 492 456 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3689
SphI G_CATG'C
Cuts at: 742 1242 742
Size: 500 3189
StyI C'CwwG_G
Cuts at: 561 823 1177 561
Size: 262 354 3073
Fragments arranged by size:
3073 354 262
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1272 1405 1864 2073 1272
Size: 133 459 209 2888
Fragments arranged by size:
2888 459 209 133
TatI w'GTAC_w
Cuts at: 3204 3204
Size: 3689
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3206 3238 3206
Size: 32 3657
Tth111I GACn'n_nGTC
Cuts at: 1486 1486
Size: 3689
XbaI T'CTAG_A
Cuts at: 720 720
Size: 3689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1184 1184
Size: 3689
XhoI C'TCGA_G
Cuts at: 388 388
Size: 3689
XmaI C'CCGG_G
Cuts at: 709 709
Size: 3689
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I ClaI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI