Restriction map for
pF1KB5048
(Circular) (Six-base) MAPSORT of: pF1KB5048.seq Check: 3132 from: 1 to: 5285
pF1KB5048 5285 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 255 337 2042 255
Size: 82 1705 3498
Fragments arranged by size:
3498 1705 82
AatII G_ACGT'C
Cuts at: 776 776
Size: 5285
AccI GT'mk_AC
Cuts at: 824 2323 824
Size: 1499 3786
Acc65I G'GTAC_C
Cuts at: 2301 2301
Size: 5285
AclI AA'CG_TT
Cuts at: 5125 5125
Size: 5285
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 321 606 823 1264 3110 3542 3895 4557 321
Size: 285 217 441 1846 432 353 662 1049
Fragments arranged by size:
1846 1049 662 441 432 353 285 217
AflIII A'CryG_T
Cuts at: 809 2637 4437 4718 809
Size: 1828 1800 281 1376
Fragments arranged by size:
1828 1800 1376 281
AleI CACnn'nnGTG
Cuts at: 371 371
Size: 5285
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 803 803
Size: 5285
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1778 1810 2140 2172 1778
Size: 32 330 32 4891
Fragments arranged by size:
4891 330 32 32
AlwNI CAG_nnn'CTG
Cuts at: 909 1185 1366 1461 2590 4028 909
Size: 276 181 95 1129 1438 2166
Fragments arranged by size:
2166 1438 1129 276 181 95
ApaI G_GGCC'C
Cuts at: 1144 1413 1733 1144
Size: 269 320 4696
Fragments arranged by size:
4696 320 269
ApaLI G'TGCA_C
Cuts at: 1699 4123 1699
Size: 2424 2861
ApoI r'AATT_y
Cuts at: 2289 4638 4894 2289
Size: 2349 256 2680
Fragments arranged by size:
2680 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5285
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5285
AvaI C'yCGr_G
Cuts at: 1012 1031 1862 2267 2305 1012
Size: 19 831 405 38 3992
Fragments arranged by size:
3992 831 405 38 19
BamHI G'GATC_C
Cuts at: 1786 2310 1786
Size: 524 4761
BanI G'GyrC_C
Cuts at: 943 2301 2715 2963 2998 943
Size: 1358 414 248 35 3230
Fragments arranged by size:
3230 1358 414 248 35
BanII G_rGCy'C
Cuts at: 1066 1144 1360 1413 1633 1733 2299 3329 1066
Size: 78 216 53 220 100 566 1030 3022
Fragments arranged by size:
3022 1030 566 220 216 100 78 53
BbeI G_GCGC'C
Cuts at: 2719 2967 2719
Size: 248 5037
BbsI GAAGACnn'nnnn_
Cuts at: 164 1028 1664 164
Size: 864 636 3785
Fragments arranged by size:
3785 864 636
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 682 716 1690 1724 2109 2143 682
Size: 34 974 34 385 34 3824
Fragments arranged by size:
3824 974 385 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3183 4239 5285 3183
Size: 1056 1046 3183
Fragments arranged by size:
3183 1056 1046
BclI T'GATC_A
Cuts at: 2805 2805
Size: 5285
BfrBI ATG'CAT
Cuts at: 780 780
Size: 5285
BglII A'GATC_T
Cuts at: 2204 2800 4837 2204
Size: 596 2037 2652
Fragments arranged by size:
2652 2037 596
BlpI GC'TnA_GC
Cuts at: 2397 2397
Size: 5285
Bme1580I G_kGCm'C
Cuts at: 946 1144 1413 1703 1733 2910 3003 4127 946
Size: 198 269 290 30 1177 93 1124 2104
Fragments arranged by size:
2104 1177 1124 290 269 198 93 30
BmgBI CAC'GTC
Cuts at: 480 480
Size: 5285
BmrI ACTGGGnnnn_n'
Cuts at: 211 1635 2497 2578 2913 5057 211
Size: 1424 862 81 335 2144 439
Fragments arranged by size:
2144 1424 862 439 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 546 2187 2256 2293 3652 546
Size: 1641 69 37 1359 2179
Fragments arranged by size:
2179 1641 1359 69 37
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 977 1617 2092 2167 193
Size: 784 640 475 75 3311
Fragments arranged by size:
3311 784 640 475 75
Bpu10I CC'TnA_GC
Cuts at: 1359 1450 1462 1359
Size: 91 12 5182
Fragments arranged by size:
5182 91 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2461 3340 4054 4352 2461
Size: 879 714 298 3394
Fragments arranged by size:
3394 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1543 4989 1543
Size: 3446 1839
BsaAI yAC'GTr
Cuts at: 812 2638 812
Size: 1826 3459
BsaBI GATnn'nnATC
Cuts at: 6 2799 4836 6
Size: 2793 2037 455
Fragments arranged by size:
2793 2037 455
BsaHI Gr'CG_yC
Cuts at: 773 2716 2964 3666 5162 773
Size: 1943 248 702 1496 896
Fragments arranged by size:
1943 1496 896 702 248
BsaWI w'CCGG_w
Cuts at: 2474 2995 3673 4084 4231 4505 2474
Size: 521 678 411 147 274 3254
Fragments arranged by size:
3254 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1048 1078 1615 1645 2140 2170 1048
Size: 30 537 30 495 30 4163
Fragments arranged by size:
4163 537 495 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1525 2122 2215 1525
Size: 597 93 4595
Fragments arranged by size:
4595 597 93
BseYI C'CCAG_C
Cuts at: 844 902 1573 2710 3468 4133 4541 844
Size: 58 671 1137 758 665 408 1588
Fragments arranged by size:
1588 1137 758 671 665 408 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2020 2020
Size: 5285
BsiEI CG_ry'CG
Cuts at: 104 2827 2873 4103 104
Size: 2723 46 1230 1286
Fragments arranged by size:
2723 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 179 1703 2299 3077 3267 4127 179
Size: 1524 596 778 190 860 1337
Fragments arranged by size:
1524 1337 860 778 596 190
BsmI GAATG_Cn'
Cuts at: 1254 1254
Size: 5285
Bsp1286I G_dGCh'C
Cuts at: 179 946 1066 1144 1360 1413 1633 1703 1733
Size: 767 120 78 216 53 220 70 30
Cuts at: 1733 2299 2910 3003 3077 3267 3329 4127 179
Size: 566 611 93 74 190 62 798 1337
Fragments arranged by size:
1337 798 767 611 566 220 216 190
120 93 78 74 70 62 53 30
BspHI T'CATG_A
Cuts at: 1340 3717 1340
Size: 2377 2908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 255 337 1016 2042 2335 2549 2851 3232 255
Size: 82 679 1026 293 214 302 381 2308
Fragments arranged by size:
2308 1026 679 381 302 293 214 82
BsrBI CCG'CTC
Cuts at: 50 2066 3577 3631 5115 5280 50
Size: 2016 1511 54 1484 165 55
Fragments arranged by size:
2016 1511 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 855 1235 1271 3197 855
Size: 380 36 1926 2943
Fragments arranged by size:
2943 1926 380 36
BsrFI r'CCGG_y
Cuts at: 1909 3283 3464 1909
Size: 1374 181 3730
Fragments arranged by size:
3730 1374 181
BssSI C'ACGA_G
Cuts at: 3556 4264 3556
Size: 708 4577
BstAPI GCAn_nnn'nTGC
Cuts at: 2662 2662
Size: 5285
BstBI TT'CG_AA
Cuts at: 3646 3646
Size: 5285
BstEII G'GTnAC_C
Cuts at: 489 489
Size: 5285
BstXI CCAn_nnnn'nTGG
Cuts at: 715 1074 1933 2145 715
Size: 359 859 212 3855
Fragments arranged by size:
3855 859 359 212
BstYI r'GATC_y
Cuts at: 1 740 1181 1316 1786 2204 2310 2777 2800
Size: 739 441 135 470 418 106 467 23
Cuts at: 2800 3135 3381 3687 3699 3785 4837 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 739 470 467 449 441 418 335
306 246 135 106 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 439 439
Size: 5285
BtgI C'CryG_G
Cuts at: 107 1067 107
Size: 960 4325
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 327 612 2028 3235 3416 3579 327
Size: 285 1416 1207 181 163 2033
Fragments arranged by size:
2033 1416 1207 285 181 163
BtsI GCAGTG_nn'
Cuts at: 1980 2528 1980
Size: 548 4737
ClaI AT'CG_AT
Cuts at: 1496 1496
Size: 5285
DraI TTT'AAA
Cuts at: 2284 2284
Size: 5285
DraIII CAC_nnn'GTG
Cuts at: 1854 2165 2217 1854
Size: 311 52 4922
Fragments arranged by size:
4922 311 52
DrdI GACnn_nn'nnGTC
Cuts at: 2991 4335 2991
Size: 1344 3941
EaeI y'GGCC_r
Cuts at: 432 836 998 1133 1836 1911 2000 2870 3044
Size: 404 162 135 703 75 89 870 174
Cuts at: 3044 3435 3462 4807 432
Size: 391 27 1345 910
Fragments arranged by size:
1345 910 870 703 404 391 174 162
135 89 75 27
EagI C'GGCC_G
Cuts at: 2870 2870
Size: 5285
EarI CTCTTCn'nnn_
Cuts at: 1286 3308 3518 1286
Size: 2022 210 3053
Fragments arranged by size:
3053 2022 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2047 3493 4235 4381 5095 2047
Size: 1446 742 146 714 2237
Fragments arranged by size:
2237 1446 742 714 146
EcoICRI GAG'CTC
Cuts at: 2297 2297
Size: 5285
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 321 606 823 977 1264 1617 2092 2167
Size: 128 285 217 154 287 353 475 75
Cuts at: 2167 3110 3542 3895 4557 193
Size: 943 432 353 662 921
Fragments arranged by size:
943 921 662 475 432 353 353 287
285 217 154 128 75
EcoNI CCTnn'n_nnAGG
Cuts at: 520 1453 520
Size: 933 4352
EcoO109I rG'GnC_Cy
Cuts at: 604 1005 1140 1141 1409 1410 1458 2235 2245
Size: 401 135 1 268 1 48 777 10
Cuts at: 2245 2424 604
Size: 179 3465
Fragments arranged by size:
3465 777 401 268 179 135 48 10
1 1
EcoRI G'AATT_C
Cuts at: 2289 2289
Size: 5285
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3304 3304
Size: 5285
FseI GG_CCGG'CC
Cuts at: 1913 1913
Size: 5285
FspI TGC'GCA
Cuts at: 3066 3066
Size: 5285
HaeII r_GCGC'y
Cuts at: 987 2719 2967 4197 4968 987
Size: 1732 248 1230 771 1304
Fragments arranged by size:
1732 1304 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 367 399 725 757 1260 1292 1615 1647 1747
Size: 32 326 32 503 32 323 32 100
Cuts at: 1747 1779 3127 3157 3159 3189 367
Size: 32 1348 30 2 30 2463
Fragments arranged by size:
2463 1348 503 326 323 100 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 228 447 753 1194 2324 228
Size: 219 306 441 1130 3189
Fragments arranged by size:
3189 1130 441 306 219
KasI G'GCGC_C
Cuts at: 2715 2963 2715
Size: 248 5037
KpnI G_GTAC'C
Cuts at: 2305 2305
Size: 5285
MluI A'CGCG_T
Cuts at: 4718 4718
Size: 5285
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4044 4228 4044
Size: 184 5101
MscI TGG'CCA
Cuts at: 838 1000 3046 838
Size: 162 2046 3077
Fragments arranged by size:
3077 2046 162
MslI CAynn'nnrTG
Cuts at: 371 713 785 961 1931 3274 3401 371
Size: 342 72 176 970 1343 127 2255
Fragments arranged by size:
2255 1343 970 342 176 127 72
MspA1I CmG'CkG
Cuts at: 502 2396 2710 3070 3854 4099 502
Size: 1894 314 360 784 245 1688
Fragments arranged by size:
1894 1688 784 360 314 245
NaeI GCC'GGC
Cuts at: 1911 3466 1911
Size: 1555 3730
NarI GG'CG_CC
Cuts at: 2716 2964 2716
Size: 248 5037
NcoI C'CATG_G
Cuts at: 107 107
Size: 5285
NgoMIV G'CCGG_C
Cuts at: 1909 3464 1909
Size: 1555 3730
NsiI A_TGCA'T
Cuts at: 782 782
Size: 5285
NspI r_CATG'y
Cuts at: 1492 2338 2838 4441 1492
Size: 846 500 1603 2336
Fragments arranged by size:
2336 1603 846 500
PciI A'CATG_T
Cuts at: 4437 4437
Size: 5285
PflMI CCAn_nnn'nTGG
Cuts at: 909 1625 909
Size: 716 4569
PmeI GTTT'AAAC
Cuts at: 2284 2284
Size: 5285
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2140 2172 3731 3763 2140
Size: 32 1559 32 3662
Fragments arranged by size:
3662 1559 32 32
PpuMI rG'GwC_Cy
Cuts at: 1005 2235 1005
Size: 1230 4055
PshAI GACnn'nnGTC
Cuts at: 1942 1942
Size: 5285
PspOMI G'GGCC_C
Cuts at: 1140 1409 1729 1140
Size: 269 320 4696
Fragments arranged by size:
4696 320 269
PstI C_TGCA'G
Cuts at: 1149 2024 2332 3017 1149
Size: 875 308 685 3417
Fragments arranged by size:
3417 875 685 308
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5285
PvuII CAG'CTG
Cuts at: 2710 3070 2710
Size: 360 4925
RsrII CG'GwC_CG
Cuts at: 3480 3480
Size: 5285
SacI G_AGCT'C
Cuts at: 2299 2299
Size: 5285
SalI G'TCGA_C
Cuts at: 2322 2322
Size: 5285
SapI GCTCTTCn'nnn_
Cuts at: 3308 3518 3308
Size: 210 5075
SbfI CC_TGCA'GG
Cuts at: 2332 2332
Size: 5285
ScaI AGT'ACT
Cuts at: 4802 4802
Size: 5285
SfcI C'TryA_G
Cuts at: 33 1145 2020 2328 3013 3981 4172 33
Size: 1112 875 308 685 968 191 1146
Fragments arranged by size:
1146 1112 968 875 685 308 191
SfoI GGC'GCC
Cuts at: 2717 2965 2717
Size: 248 5037
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5285
SmaI CCC'GGG
Cuts at: 1864 2307 1864
Size: 443 4842
SmlI C'TyrA_G
Cuts at: 2440 3355 4069 4331 2440
Size: 915 714 262 3394
Fragments arranged by size:
3394 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5285
SphI G_CATG'C
Cuts at: 2338 2838 2338
Size: 500 4785
StyI C'CwwG_G
Cuts at: 107 1001 1433 1619 1725 1736 2419 2773 107
Size: 894 432 186 106 11 683 354 2619
Fragments arranged by size:
2619 894 683 432 354 186 106 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 292 1081 1179 1567 2868 3001 3460 3669 292
Size: 789 98 388 1301 133 459 209 1908
Fragments arranged by size:
1908 1301 789 459 388 209 133 98
TatI w'GTAC_w
Cuts at: 1295 1916 4800 1295
Size: 621 2884 1780
Fragments arranged by size:
2884 1780 621
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1048 1080 1517 1549 4802 4834 1048
Size: 32 437 32 3253 32 1499
Fragments arranged by size:
3253 1499 437 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1368 3082 1368
Size: 1714 3571
XbaI T'CTAG_A
Cuts at: 2316 2316
Size: 5285
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2780 2780
Size: 5285
XmaI C'CCGG_G
Cuts at: 1862 2305 1862
Size: 443 4842
XmnI GAAnn'nnTTC
Cuts at: 1025 1025
Size: 5285
ZraI GAC'GTC
Cuts at: 774 774
Size: 5285
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PflMI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AscI AvrII BaeI BbvCI
BglI BmtI BsiWI BsmBI BspEI BsrGI BssHII BstZ17I
CspCI EcoRV FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PasI PfoI PmlI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI