Restriction map for
pF1KB5053
(Circular) (Six-base) MAPSORT of: pF1KB5053.seq Check: 6839 from: 1 to: 5453
pF1KB5053 5453 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 568 568
Size: 5453
AccI GT'mk_AC
Cuts at: 923 2491 923
Size: 1568 3885
Acc65I G'GTAC_C
Cuts at: 2469 2469
Size: 5453
AclI AA'CG_TT
Cuts at: 5293 5293
Size: 5453
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 799 874 1162 2224 2314 2341 3278 3710 4063
Size: 75 288 1062 90 27 937 432 353
Cuts at: 4063 4725 799
Size: 662 1527
Fragments arranged by size:
1527 1062 937 662 432 353 288 90
75 27
AflIII A'CryG_T
Cuts at: 2805 4605 4886 2805
Size: 1800 281 3372
Fragments arranged by size:
3372 1800 281
AleI CACnn'nnGTG
Cuts at: 991 991
Size: 5453
AlwNI CAG_nnn'CTG
Cuts at: 1300 1317 1800 1809 1906 2217 2292 2758 4196
Size: 17 483 9 97 311 75 466 1438
Cuts at: 4196 1300
Size: 2557
Fragments arranged by size:
2557 1438 483 466 311 97 75 17
9
ApaI G_GGCC'C
Cuts at: 1163 1163
Size: 5453
ApaLI G'TGCA_C
Cuts at: 4291 4291
Size: 5453
ApoI r'AATT_y
Cuts at: 1069 2457 4806 5062 1069
Size: 1388 2349 256 1460
Fragments arranged by size:
2349 1460 1388 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5453
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5453
AvaI C'yCGr_G
Cuts at: 1558 2112 2473 1558
Size: 554 361 4538
Fragments arranged by size:
4538 554 361
BamHI G'GATC_C
Cuts at: 764 2186 2478 764
Size: 1422 292 3739
Fragments arranged by size:
3739 1422 292
BanI G'GyrC_C
Cuts at: 111 580 736 1086 1966 2469 2883 3131 3166
Size: 469 156 350 880 503 414 248 35
Cuts at: 3166 111
Size: 2398
Fragments arranged by size:
2398 880 503 469 414 350 248 156
35
BanII G_rGCy'C
Cuts at: 224 817 1163 1786 2113 2467 3497 224
Size: 593 346 623 327 354 1030 2180
Fragments arranged by size:
2180 1030 623 593 354 346 327
BbeI G_GCGC'C
Cuts at: 115 2887 3135 115
Size: 2772 248 2433
Fragments arranged by size:
2772 2433 248
BbsI GAAGACnn'nnnn_
Cuts at: 1052 1052
Size: 5453
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1549 1583 1549
Size: 34 5419
BciVI GTATCCnnnnn_n'
Cuts at: 3351 4407 5453 3351
Size: 1056 1046 3351
Fragments arranged by size:
3351 1056 1046
BclI T'GATC_A
Cuts at: 2973 2973
Size: 5453
BglI GCCn_nnn'nGGC
Cuts at: 202 421 713 1312 202
Size: 219 292 599 4343
Fragments arranged by size:
4343 599 292 219
BglII A'GATC_T
Cuts at: 1409 2968 5005 1409
Size: 1559 2037 1857
Fragments arranged by size:
2037 1857 1559
BlpI GC'TnA_GC
Cuts at: 1293 2565 1293
Size: 1272 4181
Bme1580I G_kGCm'C
Cuts at: 787 1163 1657 1934 2421 3078 3171 4295 787
Size: 376 494 277 487 657 93 1124 1945
Fragments arranged by size:
1945 1124 657 494 487 376 277 93
BmgBI CAC'GTC
Cuts at: 1580 1779 1580
Size: 199 5254
BmrI ACTGGGnnnn_n'
Cuts at: 1338 2665 2746 3081 5225 1338
Size: 1327 81 335 2144 1566
Fragments arranged by size:
2144 1566 1327 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3820 3820
Size: 5453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 1343 1699 1831 1864 2155 415
Size: 928 356 132 33 291 3713
Fragments arranged by size:
3713 928 356 291 132 33
Bpu10I CC'TnA_GC
Cuts at: 1027 1027
Size: 5453
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1315 1434 1606 1903 2629 3508 4222 4520 1315
Size: 119 172 297 726 879 714 298 2248
Fragments arranged by size:
2248 879 726 714 298 297 172 119
BsaI GGTCTCn'nnnn_
Cuts at: 1555 1630 2188 2350 5157 1555
Size: 75 558 162 2807 1851
Fragments arranged by size:
2807 1851 558 162 75
BsaAI yAC'GTr
Cuts at: 2806 2806
Size: 5453
BsaBI GATnn'nnATC
Cuts at: 6 1861 2967 5004 6
Size: 1855 1106 2037 455
Fragments arranged by size:
2037 1855 1106 455
BsaHI Gr'CG_yC
Cuts at: 112 2884 3132 3834 5330 112
Size: 2772 248 702 1496 235
Fragments arranged by size:
2772 1496 702 248 235
BsaWI w'CCGG_w
Cuts at: 823 1686 2642 3163 3841 4252 4399 4673 823
Size: 863 956 521 678 411 147 274 1603
Fragments arranged by size:
1603 956 863 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 766 796 916 946 1341 1371 766
Size: 30 120 30 395 30 4848
Fragments arranged by size:
4848 395 120 30 30 30
BseYI C'CCAG_C
Cuts at: 760 2878 3636 4301 4709 760
Size: 2118 758 665 408 1504
Fragments arranged by size:
2118 1504 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 921 989 1245 921
Size: 68 256 5129
Fragments arranged by size:
5129 256 68
BsiEI CG_ry'CG
Cuts at: 104 292 385 1041 2995 3041 4271 104
Size: 188 93 656 1954 46 1230 1286
Fragments arranged by size:
1954 1286 1230 656 188 93 46
BsiHKAI G_wGCw'C
Cuts at: 2467 3245 3435 4295 2467
Size: 778 190 860 3625
Fragments arranged by size:
3625 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 2084 2380 2084
Size: 296 5157
Bsp1286I G_dGCh'C
Cuts at: 224 787 817 1163 1657 1786 1934 2113 2421
Size: 563 30 346 494 129 148 179 308
Cuts at: 2421 2467 3078 3171 3245 3435 3497 4295 224
Size: 46 611 93 74 190 62 798 1382
Fragments arranged by size:
1382 798 611 563 494 346 308 190
179 148 129 93 74 62 46 30
BspEI T'CCGG_A
Cuts at: 1686 1686
Size: 5453
BspHI T'CATG_A
Cuts at: 3885 3885
Size: 5453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 254 380 533 568 1859 1964 2503 2717 3019
Size: 126 153 35 1291 105 539 214 302
Cuts at: 3019 3400 254
Size: 381 2307
Fragments arranged by size:
2307 1291 539 381 302 214 153 126
105 35
BsrBI CCG'CTC
Cuts at: 50 1392 3745 3799 5283 5448 50
Size: 1342 2353 54 1484 165 55
Fragments arranged by size:
2353 1484 1342 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1049 1607 2013 3365 1049
Size: 558 406 1352 3137
Fragments arranged by size:
3137 1352 558 406
BsrFI r'CCGG_y
Cuts at: 287 1036 3451 3632 287
Size: 749 2415 181 2108
Fragments arranged by size:
2415 2108 749 181
BssSI C'ACGA_G
Cuts at: 2191 3724 4432 2191
Size: 1533 708 3212
Fragments arranged by size:
3212 1533 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2830 2830
Size: 5453
BstBI TT'CG_AA
Cuts at: 3814 3814
Size: 5453
BstXI CCAn_nnnn'nTGG
Cuts at: 916 916
Size: 5453
BstYI r'GATC_y
Cuts at: 1 613 764 1373 1409 1419 1856 2186 2478
Size: 612 151 609 36 10 437 330 292
Cuts at: 2478 2945 2968 3303 3549 3855 3867 3953 5005
Size: 467 23 335 246 306 12 86 1052
Cuts at: 5005 1
Size: 449
Fragments arranged by size:
1052 612 609 467 449 437 335 330
306 292 246 151 86 36 23 12
10
BtgI C'CryG_G
Cuts at: 107 909 1323 1926 107
Size: 802 414 603 3634
Fragments arranged by size:
3634 802 603 414
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 220 3403 3584 3747 220
Size: 3183 181 163 1926
Fragments arranged by size:
3183 1926 181 163
BtsI GCAGTG_nn'
Cuts at: 2042 2421 2696 2042
Size: 379 275 4799
Fragments arranged by size:
4799 379 275
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1431 1466 1908 1943 1431
Size: 35 442 35 4941
Fragments arranged by size:
4941 442 35 35
DraI TTT'AAA
Cuts at: 2452 2452
Size: 5453
DraIII CAC_nnn'GTG
Cuts at: 1086 1188 2268 1086
Size: 102 1080 4271
Fragments arranged by size:
4271 1080 102
DrdI GACnn_nn'nnGTC
Cuts at: 3159 4503 3159
Size: 1344 4109
EaeI y'GGCC_r
Cuts at: 289 382 395 422 1038 1304 1439 1464 1604
Size: 93 13 27 616 266 135 25 140
Cuts at: 1604 3038 3212 3603 3630 4975 289
Size: 1434 174 391 27 1345 767
Fragments arranged by size:
1434 1345 767 616 391 266 174 140
135 93 27 27 25 13
EagI C'GGCC_G
Cuts at: 289 382 1038 3038 289
Size: 93 656 2000 2704
Fragments arranged by size:
2704 2000 656 93
EarI CTCTTCn'nnn_
Cuts at: 3476 3686 3476
Size: 210 5243
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3661 4403 4549 5263 3661
Size: 742 146 714 3851
Fragments arranged by size:
3851 742 714 146
EcoICRI GAG'CTC
Cuts at: 2465 2465
Size: 5453
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 799 874 1162 1343 1699 1831 1864 2155
Size: 384 75 288 181 356 132 33 291
Cuts at: 2155 2224 2314 2341 3278 3710 4063 4725 415
Size: 69 90 27 937 432 353 662 1143
Fragments arranged by size:
1143 937 662 432 384 356 353 291
288 181 132 90 75 69 33 27
EcoNI CCTnn'n_nnAGG
Cuts at: 1524 1864 1524
Size: 340 5113
EcoO109I rG'GnC_Cy
Cuts at: 1160 1839 1848 2214 2289 2348 2592 1160
Size: 679 9 366 75 59 244 4021
Fragments arranged by size:
4021 679 366 244 75 59 9
EcoRI G'AATT_C
Cuts at: 1069 2457 1069
Size: 1388 4065
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1098 3472 1098
Size: 2374 3079
FspI TGC'GCA
Cuts at: 2005 3234 2005
Size: 1229 4224
HaeII r_GCGC'y
Cuts at: 115 1293 1800 2887 3135 4365 5136 115
Size: 1178 507 1087 248 1230 771 432
Fragments arranged by size:
1230 1178 1087 771 507 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 605 637 801 833 916 948 1882 1914 1930
Size: 32 164 32 83 32 934 32 16
Cuts at: 1930 1962 3295 3325 3327 3357 605
Size: 32 1333 30 2 30 2701
Fragments arranged by size:
2701 1333 934 164 83 32 32 32
32 32 30 30 16 2
HincII GTy'rAC
Cuts at: 2492 2492
Size: 5453
KasI G'GCGC_C
Cuts at: 111 2883 3131 111
Size: 2772 248 2433
Fragments arranged by size:
2772 2433 248
KpnI G_GTAC'C
Cuts at: 2473 2473
Size: 5453
MluI A'CGCG_T
Cuts at: 4886 4886
Size: 5453
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1973 4212 4396 1973
Size: 2239 184 3030
Fragments arranged by size:
3030 2239 184
MscI TGG'CCA
Cuts at: 397 424 1306 1441 1466 1606 3214 397
Size: 27 882 135 25 140 1608 2636
Fragments arranged by size:
2636 1608 882 140 135 27 25
MslI CAynn'nnrTG
Cuts at: 329 943 991 1154 1240 3442 3569 329
Size: 614 48 163 86 2202 127 2213
Fragments arranged by size:
2213 2202 614 163 127 86 48
MspA1I CmG'CkG
Cuts at: 294 446 1300 1763 1903 2564 2878 3238 4022
Size: 152 854 463 140 661 314 360 784
Cuts at: 4022 4267 294
Size: 245 1480
Fragments arranged by size:
1480 854 784 661 463 360 314 245
152 140
NaeI GCC'GGC
Cuts at: 3634 3634
Size: 5453
NarI GG'CG_CC
Cuts at: 112 2884 3132 112
Size: 2772 248 2433
Fragments arranged by size:
2772 2433 248
NcoI C'CATG_G
Cuts at: 107 1323 107
Size: 1216 4237
NgoMIV G'CCGG_C
Cuts at: 3632 3632
Size: 5453
NspI r_CATG'y
Cuts at: 1774 2128 2506 3006 4609 1774
Size: 354 378 500 1603 2618
Fragments arranged by size:
2618 1603 500 378 354
PasI CC'CwG_GG
Cuts at: 770 1723 770
Size: 953 4500
PciI A'CATG_T
Cuts at: 4605 4605
Size: 5453
PfoI T'CCnGG_A
Cuts at: 1808 1808
Size: 5453
PmeI GTTT'AAAC
Cuts at: 2452 2452
Size: 5453
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3899 3931 3899
Size: 32 5421
PpuMI rG'GwC_Cy
Cuts at: 1839 1848 2348 1839
Size: 9 500 4944
Fragments arranged by size:
4944 500 9
PsiI TTA'TAA
Cuts at: 560 560
Size: 5453
PspOMI G'GGCC_C
Cuts at: 1159 1159
Size: 5453
PspXI vC'TCGA_Gb
Cuts at: 2112 2112
Size: 5453
PstI C_TGCA'G
Cuts at: 143 579 1805 1856 2500 3185 143
Size: 436 1226 51 644 685 2411
Fragments arranged by size:
2411 1226 685 644 436 51
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5453
PvuII CAG'CTG
Cuts at: 1300 1903 2878 3238 1300
Size: 603 975 360 3515
Fragments arranged by size:
3515 975 603 360
RsrII CG'GwC_CG
Cuts at: 387 3648 387
Size: 3261 2192
SacI G_AGCT'C
Cuts at: 2467 2467
Size: 5453
SalI G'TCGA_C
Cuts at: 2490 2490
Size: 5453
SanDI GG'GwC_CC
Cuts at: 2348 2348
Size: 5453
SapI GCTCTTCn'nnn_
Cuts at: 3476 3686 3476
Size: 210 5243
SbfI CC_TGCA'GG
Cuts at: 2500 2500
Size: 5453
ScaI AGT'ACT
Cuts at: 4970 4970
Size: 5453
SfcI C'TryA_G
Cuts at: 33 117 139 575 1801 1852 2496 3181 4149
Size: 84 22 436 1226 51 644 685 968
Cuts at: 4149 4340 33
Size: 191 1146
Fragments arranged by size:
1226 1146 968 685 644 436 191 84
51 22
SfiI GGCCn_nnn'nGGCC
Cuts at: 421 421
Size: 5453
SfoI GGC'GCC
Cuts at: 113 2885 3133 113
Size: 2772 248 2433
Fragments arranged by size:
2772 2433 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5453
SmaI CCC'GGG
Cuts at: 2475 2475
Size: 5453
SmlI C'TyrA_G
Cuts at: 1330 1413 1621 1918 2112 2608 3523 4237 4499
Size: 83 208 297 194 496 915 714 262
Cuts at: 4499 1330
Size: 2284
Fragments arranged by size:
2284 915 714 496 297 262 208 194
83
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5453
SphI G_CATG'C
Cuts at: 2128 2506 3006 2128
Size: 378 500 4575
Fragments arranged by size:
4575 500 378
StyI C'CwwG_G
Cuts at: 107 197 455 536 708 788 1323 1751 2148
Size: 90 258 81 172 80 535 428 397
Cuts at: 2148 2587 2941 107
Size: 439 354 2619
Fragments arranged by size:
2619 535 439 428 397 354 258 172
90 81 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 142 852 955 1414 1654 1760 1764 2067 3036
Size: 710 103 459 240 106 4 303 969
Cuts at: 3036 3169 3628 3837 142
Size: 133 459 209 1758
Fragments arranged by size:
1758 969 710 459 459 303 240 209
133 106 103 4
TatI w'GTAC_w
Cuts at: 230 4968 230
Size: 4738 715
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 766 798 1341 1373 4970 5002 766
Size: 32 543 32 3597 32 1217
Fragments arranged by size:
3597 1217 543 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3250 3250
Size: 5453
XbaI T'CTAG_A
Cuts at: 2484 2484
Size: 5453
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 591 694 1314 2948 591
Size: 103 620 1634 3096
Fragments arranged by size:
3096 1634 620 103
XhoI C'TCGA_G
Cuts at: 2112 2112
Size: 5453
XmaI C'CCGG_G
Cuts at: 2473 2473
Size: 5453
XmnI GAAnn'nnTTC
Cuts at: 2299 2299
Size: 5453
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PpuMI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BmtI BseRI BsiWI BsmI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PmlI PshAI PsrI SacII SexAI
SgrAI SnaBI SrfI SspI StuI SwaI ZraI