Restriction map for
pF1KB5057
(Circular) (Six-base) MAPSORT of: pF1KB5057.seq Check: 5326 from: 1 to: 4712
pF1KB5057 4712 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 183 183
Size: 4712
AccI GT'mk_AC
Cuts at: 1750 1750
Size: 4712
Acc65I G'GTAC_C
Cuts at: 1728 1728
Size: 4712
AclI AA'CG_TT
Cuts at: 4552 4552
Size: 4712
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 1449 1706 2537 2969 3716 3984 1216
Size: 233 257 831 432 747 268 1944
Fragments arranged by size:
1944 831 747 432 268 257 233
AflIII A'CryG_T
Cuts at: 887 2064 3168 4145 887
Size: 1177 1104 977 1454
Fragments arranged by size:
1454 1177 1104 977
AgeI A'CCGG_T
Cuts at: 3100 3100
Size: 4712
AleI CACnn'nnGTG
Cuts at: 280 343 959 280
Size: 63 616 4033
Fragments arranged by size:
4033 616 63
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1510 1510
Size: 4712
AlwNI CAG_nnn'CTG
Cuts at: 678 2017 3584 678
Size: 1339 1567 1806
Fragments arranged by size:
1806 1567 1339
ApaI G_GGCC'C
Cuts at: 852 852
Size: 4712
ApaLI G'TGCA_C
Cuts at: 721 3482 721
Size: 2761 1951
ApoI r'AATT_y
Cuts at: 554 1716 4065 4321 554
Size: 1162 2349 256 945
Fragments arranged by size:
2349 1162 945 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4712
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4712
AvaI C'yCGr_G
Cuts at: 1732 1732
Size: 4712
BamHI G'GATC_C
Cuts at: 1737 1737
Size: 4712
BanI G'GyrC_C
Cuts at: 835 961 1393 1728 2142 2390 2425 835
Size: 126 432 335 414 248 35 3122
Fragments arranged by size:
3122 432 414 335 248 126 35
BanII G_rGCy'C
Cuts at: 852 1451 1726 2756 852
Size: 599 275 1030 2808
Fragments arranged by size:
2808 1030 599 275
BbeI G_GCGC'C
Cuts at: 839 1397 2146 2394 839
Size: 558 749 248 3157
Fragments arranged by size:
3157 749 558 248
BbsI GAAGACnn'nnnn_
Cuts at: 622 1455 622
Size: 833 3879
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 735 769 735
Size: 34 4678
BciVI GTATCCnnnnn_n'
Cuts at: 2610 3371 4712 2610
Size: 761 1341 2610
Fragments arranged by size:
2610 1341 761
BclI T'GATC_A
Cuts at: 446 2232 446
Size: 1786 2926
BglII A'GATC_T
Cuts at: 170 692 2227 4264 170
Size: 522 1535 2037 618
Fragments arranged by size:
2037 1535 618 522
BlpI GC'TnA_GC
Cuts at: 1286 1824 1286
Size: 538 4174
Bme1580I G_kGCm'C
Cuts at: 725 813 852 2337 2430 3486 725
Size: 88 39 1485 93 1056 1951
Fragments arranged by size:
1951 1485 1056 93 88 39
BmgBI CAC'GTC
Cuts at: 888 1629 888
Size: 741 3971
BmrI ACTGGGnnnn_n'
Cuts at: 771 1924 2005 2340 4484 771
Size: 1153 81 335 2144 999
Fragments arranged by size:
2144 1153 999 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3079 3079
Size: 4712
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 382 706 301
Size: 81 324 4307
Fragments arranged by size:
4307 324 81
Bpu10I CC'TnA_GC
Cuts at: 214 1216 214
Size: 1002 3710
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1888 2767 3259 3557 1888
Size: 879 492 298 3043
Fragments arranged by size:
3043 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 657 1612 4416 657
Size: 955 2804 953
Fragments arranged by size:
2804 955 953
BsaAI yAC'GTr
Cuts at: 2065 2065
Size: 4712
BsaBI GATnn'nnATC
Cuts at: 6 2226 4263 6
Size: 2220 2037 455
Fragments arranged by size:
2220 2037 455
BsaHI Gr'CG_yC
Cuts at: 836 1394 2143 2391 3093 4589 836
Size: 558 749 248 702 1496 959
Fragments arranged by size:
1496 959 749 702 558 248
BsaWI w'CCGG_w
Cuts at: 1511 1901 2422 3100 3374 3521 3932 1511
Size: 390 521 678 274 147 411 2291
Fragments arranged by size:
2291 678 521 411 390 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 809 839 809
Size: 30 4682
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 703 719 1632 238
Size: 465 16 913 3318
Fragments arranged by size:
3318 913 465 16
BseYI C'CCAG_C
Cuts at: 1147 1204 1654 2137 2895 3472 3968 1147
Size: 57 450 483 758 577 496 1891
Fragments arranged by size:
1891 758 577 496 483 450 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 863 1325 863
Size: 462 4250
BsiEI CG_ry'CG
Cuts at: 104 2254 2300 3508 104
Size: 2150 46 1208 1308
Fragments arranged by size:
2150 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 137 725 1726 2504 2694 3486 137
Size: 588 1001 778 190 792 1363
Fragments arranged by size:
1363 1001 792 778 588 190
BsmI GAATG_Cn'
Cuts at: 1062 1206 1372 1062
Size: 144 166 4402
Fragments arranged by size:
4402 166 144
Bsp1286I G_dGCh'C
Cuts at: 137 725 813 852 1451 1726 2337 2430 2504
Size: 588 88 39 599 275 611 93 74
Cuts at: 2504 2694 2756 3486 137
Size: 190 62 730 1363
Fragments arranged by size:
1363 730 611 599 588 275 190 93
88 74 62 39
BspEI T'CCGG_A
Cuts at: 1511 3932 1511
Size: 2421 2291
BspHI T'CATG_A
Cuts at: 3888 3888
Size: 4712
BspMI ACCTGCnnnn'nnnn_
Cuts at: 183 1502 1762 1976 2278 2659 183
Size: 1319 260 214 302 381 2236
Fragments arranged by size:
2236 1319 381 302 260 214
BsrBI CCG'CTC
Cuts at: 50 510 3004 3058 4542 4707 50
Size: 460 2494 54 1484 165 55
Fragments arranged by size:
2494 1484 460 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1541 2624 1541
Size: 1083 3629
BsrFI r'CCGG_y
Cuts at: 2710 2891 3100 2710
Size: 181 209 4322
Fragments arranged by size:
4322 209 181
BssSI C'ACGA_G
Cuts at: 2983 3341 2983
Size: 358 4354
BstAPI GCAn_nnn'nTGC
Cuts at: 2089 2089
Size: 4712
BstBI TT'CG_AA
Cuts at: 3073 3073
Size: 4712
BstEII G'GTnAC_C
Cuts at: 282 282
Size: 4712
BstYI r'GATC_y
Cuts at: 1 170 357 692 1737 2204 2227 2562 2808
Size: 169 187 335 1045 467 23 335 246
Cuts at: 2808 3820 3906 3918 4264 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1045 1012 467 449 346 335 335 246
187 169 86 23 12
BtgI C'CryG_G
Cuts at: 107 813 907 1379 107
Size: 706 94 472 3440
Fragments arranged by size:
3440 706 472 94
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2662 2843 3006 2662
Size: 181 163 4368
Fragments arranged by size:
4368 181 163
BtsI GCAGTG_nn'
Cuts at: 1955 1955
Size: 4712
DraI TTT'AAA
Cuts at: 1711 1711
Size: 4712
DrdI GACnn_nn'nnGTC
Cuts at: 2418 3276 2418
Size: 858 3854
EaeI y'GGCC_r
Cuts at: 458 861 1376 2297 2471 2862 2889 4234 458
Size: 403 515 921 174 391 27 1345 936
Fragments arranged by size:
1345 936 921 515 403 391 174 27
EagI C'GGCC_G
Cuts at: 2297 2297
Size: 4712
EarI CTCTTCn'nnn_
Cuts at: 893 2735 2945 893
Size: 1842 210 2660
Fragments arranged by size:
2660 1842 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1279 2920 3230 3376 4522 1279
Size: 1641 310 146 1146 1469
Fragments arranged by size:
1641 1469 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1724 1724
Size: 4712
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 382 706 1216 1449 1706 2537 2969 3716
Size: 81 324 510 233 257 831 432 747
Cuts at: 3716 3984 301
Size: 268 1029
Fragments arranged by size:
1029 831 747 510 432 324 268 257
233 81
EcoNI CCTnn'n_nnAGG
Cuts at: 1246 1564 1246
Size: 318 4394
EcoO109I rG'GnC_Cy
Cuts at: 849 1065 1151 1491 1851 849
Size: 216 86 340 360 3710
Fragments arranged by size:
3710 360 340 216 86
EcoRI G'AATT_C
Cuts at: 554 1716 554
Size: 1162 3550
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 402 2731 402
Size: 2329 2383
FspI TGC'GCA
Cuts at: 2493 2493
Size: 4712
HaeII r_GCGC'y
Cuts at: 839 1397 2146 2394 3416 4395 839
Size: 558 749 248 1022 979 1156
Fragments arranged by size:
1156 1022 979 749 558 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 684 716 2554 2584 2586 2616 684
Size: 32 1838 30 2 30 2780
Fragments arranged by size:
2780 1838 32 30 30 2
HincII GTy'rAC
Cuts at: 1751 1751
Size: 4712
KasI G'GCGC_C
Cuts at: 835 1393 2142 2390 835
Size: 558 749 248 3157
Fragments arranged by size:
3157 749 558 248
KpnI G_GTAC'C
Cuts at: 1732 1732
Size: 4712
MluI A'CGCG_T
Cuts at: 4145 4145
Size: 4712
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3383 3567 3383
Size: 184 4528
MscI TGG'CCA
Cuts at: 460 863 2473 460
Size: 403 1610 2699
Fragments arranged by size:
2699 1610 403
MslI CAynn'nnrTG
Cuts at: 280 343 718 935 959 1171 2701 2828 280
Size: 63 375 217 24 212 1530 127 2164
Fragments arranged by size:
2164 1530 375 217 212 127 63 24
MspA1I CmG'CkG
Cuts at: 289 307 678 947 989 1823 2137 2497 3510
Size: 18 371 269 42 834 314 360 1013
Cuts at: 3510 3755 289
Size: 245 1246
Fragments arranged by size:
1246 1013 834 371 360 314 269 245
42 18
NaeI GCC'GGC
Cuts at: 2893 2893
Size: 4712
NarI GG'CG_CC
Cuts at: 836 1394 2143 2391 836
Size: 558 749 248 3157
Fragments arranged by size:
3157 749 558 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4712
NgoMIV G'CCGG_C
Cuts at: 2891 2891
Size: 4712
NspI r_CATG'y
Cuts at: 1681 1765 2265 3172 1681
Size: 84 500 907 3221
Fragments arranged by size:
3221 907 500 84
PasI CC'CwG_GG
Cuts at: 549 549
Size: 4712
PciI A'CATG_T
Cuts at: 3168 3168
Size: 4712
PflMI CCAn_nnn'nTGG
Cuts at: 324 1100 1410 324
Size: 776 310 3626
Fragments arranged by size:
3626 776 310
PmeI GTTT'AAAC
Cuts at: 1711 1711
Size: 4712
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 3851
Size: 32 4680
PpuMI rG'GwC_Cy
Cuts at: 1151 1491 1151
Size: 340 4372
PspOMI G'GGCC_C
Cuts at: 848 848
Size: 4712
PstI C_TGCA'G
Cuts at: 194 245 857 1445 1759 2444 194
Size: 51 612 588 314 685 2462
Fragments arranged by size:
2462 685 612 588 314 51
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4712
PvuII CAG'CTG
Cuts at: 307 678 947 2137 2497 307
Size: 371 269 1190 360 2522
Fragments arranged by size:
2522 1190 371 360 269
RsrII CG'GwC_CG
Cuts at: 2907 2907
Size: 4712
SacI G_AGCT'C
Cuts at: 1726 1726
Size: 4712
SalI G'TCGA_C
Cuts at: 1749 1749
Size: 4712
SanDI GG'GwC_CC
Cuts at: 1151 1151
Size: 4712
SapI GCTCTTCn'nnn_
Cuts at: 893 2735 2945 893
Size: 1842 210 2660
Fragments arranged by size:
2660 1842 210
SbfI CC_TGCA'GG
Cuts at: 1759 1759
Size: 4712
ScaI AGT'ACT
Cuts at: 4229 4229
Size: 4712
SfcI C'TryA_G
Cuts at: 33 190 241 620 853 1441 1755 2440 3433
Size: 157 51 379 233 588 314 685 993
Cuts at: 3433 3624 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 588 379 314 233 191
157 51
SfoI GGC'GCC
Cuts at: 837 1395 2144 2392 837
Size: 558 749 248 3157
Fragments arranged by size:
3157 749 558 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4712
SmaI CCC'GGG
Cuts at: 1734 1734
Size: 4712
SmlI C'TyrA_G
Cuts at: 1867 2782 3274 3536 1867
Size: 915 492 262 3043
Fragments arranged by size:
3043 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4712
SphI G_CATG'C
Cuts at: 1765 2265 1765
Size: 500 4212
StuI AGG'CCT
Cuts at: 257 257
Size: 4712
StyI C'CwwG_G
Cuts at: 107 278 920 971 1099 1422 1846 2200 107
Size: 171 642 51 128 323 424 354 2619
Fragments arranged by size:
2619 642 424 354 323 171 128 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 949 2295 2428 2887 3096 949
Size: 1346 133 459 209 2565
Fragments arranged by size:
2565 1346 459 209 133
TatI w'GTAC_w
Cuts at: 1437 4227 1437
Size: 2790 1922
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 809 841 4229 4261 809
Size: 32 3388 32 1260
Fragments arranged by size:
3388 1260 32 32
Tth111I GACn'n_nGTC
Cuts at: 2509 2509
Size: 4712
XbaI T'CTAG_A
Cuts at: 1743 1743
Size: 4712
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2207 2207
Size: 4712
XmaI C'CCGG_G
Cuts at: 1732 1732
Size: 4712
XmnI GAAnn'nnTTC
Cuts at: 517 517
Size: 4712
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbvCI BfrBI BglI BmtI BsiWI BsmBI BsrGI BssHII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PshAI PsiI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI