Restriction map for
pF1KB5063
(Circular) (Six-base) MAPSORT of: pF1KB5063.seq Check: 9260 from: 1 to: 5438
pF1KB5063 5438 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AatII G_ACGT'C
Cuts at: 1535 1535
Size: 5438
AccI GT'mk_AC
Cuts at: 1372 2476 1372
Size: 1104 4334
Acc65I G'GTAC_C
Cuts at: 2454 2454
Size: 5438
AclI AA'CG_TT
Cuts at: 528 5278 528
Size: 4750 688
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 598 2231 2431 3263 3695 4048 4710 598
Size: 1633 200 832 432 353 662 1326
Fragments arranged by size:
1633 1326 832 662 432 353 200
AfeI AGC'GCT
Cuts at: 372 372
Size: 5438
AflII C'TTAA_G
Cuts at: 793 793
Size: 5438
AflIII A'CryG_T
Cuts at: 2268 2790 4590 4871 2268
Size: 522 1800 281 2835
Fragments arranged by size:
2835 1800 522 281
AhdI GACnn_n'nnGTC
Cuts at: 126 126
Size: 5438
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 655 687 1030 1062 655
Size: 32 343 32 5031
Fragments arranged by size:
5031 343 32 32
AlwNI CAG_nnn'CTG
Cuts at: 237 519 759 876 2116 2743 4181 237
Size: 282 240 117 1240 627 1438 1494
Fragments arranged by size:
1494 1438 1240 627 282 240 117
ApaI G_GGCC'C
Cuts at: 643 2068 643
Size: 1425 4013
ApaLI G'TGCA_C
Cuts at: 4276 4276
Size: 5438
ApoI r'AATT_y
Cuts at: 1483 2442 4791 5047 1483
Size: 959 2349 256 1874
Fragments arranged by size:
2349 1874 959 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5438
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5438
AvaI C'yCGr_G
Cuts at: 566 1718 2458 566
Size: 1152 740 3546
Fragments arranged by size:
3546 1152 740
BamHI G'GATC_C
Cuts at: 266 2361 2463 266
Size: 2095 102 3241
Fragments arranged by size:
3241 2095 102
BanI G'GyrC_C
Cuts at: 503 1881 2226 2454 2868 3116 3151 503
Size: 1378 345 228 414 248 35 2790
Fragments arranged by size:
2790 1378 414 345 248 228 35
BanII G_rGCy'C
Cuts at: 362 643 1154 1202 2068 2452 3482 362
Size: 281 511 48 866 384 1030 2318
Fragments arranged by size:
2318 1030 866 511 384 281 48
BbeI G_GCGC'C
Cuts at: 2872 3120 2872
Size: 248 5190
BbsI GAAGACnn'nnnn_
Cuts at: 1935 2423 1935
Size: 488 4950
BciVI GTATCCnnnnn_n'
Cuts at: 294 3336 4392 5438 294
Size: 3042 1056 1046 294
Fragments arranged by size:
3042 1056 1046 294
BclI T'GATC_A
Cuts at: 599 2958 599
Size: 2359 3079
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5438
BglII A'GATC_T
Cuts at: 2953 4990 2953
Size: 2037 3401
BlpI GC'TnA_GC
Cuts at: 227 367 2550 227
Size: 140 2183 3115
Fragments arranged by size:
3115 2183 140
Bme1580I G_kGCm'C
Cuts at: 643 1719 1886 2068 3063 3156 4280 643
Size: 1076 167 182 995 93 1124 1801
Fragments arranged by size:
1801 1124 1076 995 182 167 93
BmrI ACTGGGnnnn_n'
Cuts at: 1647 2063 2154 2650 2731 3066 5210 1647
Size: 416 91 496 81 335 2144 1875
Fragments arranged by size:
2144 1875 496 416 335 91 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1216 3805 1216
Size: 2589 2849
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 898 1251 1591 1843 2110 2138 682
Size: 216 353 340 252 267 28 3982
Fragments arranged by size:
3982 353 340 267 252 216 28
Bpu10I CC'TnA_GC
Cuts at: 151 151
Size: 5438
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 400 2614 3493 4207 4505 163
Size: 237 2214 879 714 298 1096
Fragments arranged by size:
2214 1096 879 714 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 1229 1897 5142 1229
Size: 668 3245 1525
Fragments arranged by size:
3245 1525 668
BsaAI yAC'GTr
Cuts at: 901 2271 2791 901
Size: 1370 520 3548
Fragments arranged by size:
3548 1370 520
BsaBI GATnn'nnATC
Cuts at: 6 2952 4989 6
Size: 2946 2037 455
Fragments arranged by size:
2946 2037 455
BsaHI Gr'CG_yC
Cuts at: 309 1532 2869 3117 3819 5315 309
Size: 1223 1337 248 702 1496 432
Fragments arranged by size:
1496 1337 1223 702 432 248
BsaWI w'CCGG_w
Cuts at: 707 2627 3148 3826 4237 4384 4658 707
Size: 1920 521 678 411 147 274 1487
Fragments arranged by size:
1920 1487 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 655 685 1224 1254 1718 1748 655
Size: 30 539 30 464 30 4345
Fragments arranged by size:
4345 539 464 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 490 1205 1210 1264 1984 490
Size: 715 5 54 720 3944
Fragments arranged by size:
3944 720 715 54 5
BseYI C'CCAG_C
Cuts at: 362 512 1893 2863 3621 4286 4694 362
Size: 150 1381 970 758 665 408 1106
Fragments arranged by size:
1381 1106 970 758 665 408 150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1458 1558 1567 1458
Size: 100 9 5329
Fragments arranged by size:
5329 100 9
BsiEI CG_ry'CG
Cuts at: 104 2980 3026 4256 104
Size: 2876 46 1230 1286
Fragments arranged by size:
2876 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1154 1202 1457 2452 3230 3420 4280 1154
Size: 48 255 995 778 190 860 2312
Fragments arranged by size:
2312 995 860 778 255 190 48
BsmBI CGTCTCn'nnnn_
Cuts at: 166 316 166
Size: 150 5288
Bsp1286I G_dGCh'C
Cuts at: 362 643 1154 1202 1457 1719 1886 2068 2452
Size: 281 511 48 255 262 167 182 384
Cuts at: 2452 3063 3156 3230 3420 3482 4280 362
Size: 611 93 74 190 62 798 1520
Fragments arranged by size:
1520 798 611 511 384 281 262 255
190 182 167 93 74 62 48
BspHI T'CATG_A
Cuts at: 3870 3870
Size: 5438
BspMI ACCTGCnnnn'nnnn_
Cuts at: 612 885 1580 2107 2488 2702 3004 3385 612
Size: 273 695 527 381 214 302 381 2665
Fragments arranged by size:
2665 695 527 381 381 302 273 214
BsrBI CCG'CTC
Cuts at: 50 432 3730 3784 5268 5433 50
Size: 382 3298 54 1484 165 55
Fragments arranged by size:
3298 1484 382 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1280 1527 3350 1280
Size: 247 1823 3368
Fragments arranged by size:
3368 1823 247
BsrFI r'CCGG_y
Cuts at: 506 3436 3617 506
Size: 2930 181 2327
Fragments arranged by size:
2930 2327 181
BssHII G'CGCG_C
Cuts at: 197 197
Size: 5438
BssSI C'ACGA_G
Cuts at: 1805 3709 4417 1805
Size: 1904 708 2826
Fragments arranged by size:
2826 1904 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1624 2815 1624
Size: 1191 4247
BstBI TT'CG_AA
Cuts at: 3799 3799
Size: 5438
BstEII G'GTnAC_C
Cuts at: 2094 2094
Size: 5438
BstXI CCAn_nnnn'nTGG
Cuts at: 808 1869 808
Size: 1061 4377
BstYI r'GATC_y
Cuts at: 1 266 347 1601 2214 2361 2463 2930 2953
Size: 265 81 1254 613 147 102 467 23
Cuts at: 2953 3288 3534 3840 3852 3938 4990 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1254 1052 613 467 449 335 306 265
246 147 102 86 81 23 12
BstZ17I GTA'TAC
Cuts at: 1373 1373
Size: 5438
Bsu36I CC'TnA_GG
Cuts at: 1694 2020 1694
Size: 326 5112
BtgI C'CryG_G
Cuts at: 107 1244 1648 1811 2357 107
Size: 1137 404 163 546 3188
Fragments arranged by size:
3188 1137 546 404 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3388 3569 3732 3388
Size: 181 163 5094
Fragments arranged by size:
5094 181 163
BtsI GCAGTG_nn'
Cuts at: 1255 1431 1555 2681 1255
Size: 176 124 1126 4012
Fragments arranged by size:
4012 1126 176 124
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 707 742 707
Size: 35 5403
DraI TTT'AAA
Cuts at: 2437 2437
Size: 5438
DraIII CAC_nnn'GTG
Cuts at: 2148 2274 2148
Size: 126 5312
DrdI GACnn_nn'nnGTC
Cuts at: 622 3144 4488 622
Size: 2522 1344 1572
Fragments arranged by size:
2522 1572 1344
EaeI y'GGCC_r
Cuts at: 1055 1560 3023 3197 3588 3615 4960 1055
Size: 505 1463 174 391 27 1345 1533
Fragments arranged by size:
1533 1463 1345 505 391 174 27
EagI C'GGCC_G
Cuts at: 3023 3023
Size: 5438
EarI CTCTTCn'nnn_
Cuts at: 139 1506 3461 3671 139
Size: 1367 1955 210 1906
Fragments arranged by size:
1955 1906 1367 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3646 4388 4534 5248 3646
Size: 742 146 714 3836
Fragments arranged by size:
3836 742 714 146
EcoICRI GAG'CTC
Cuts at: 1152 1200 2450 1152
Size: 48 1250 4140
Fragments arranged by size:
4140 1250 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 598 682 898 1251 1591 1843 2110 2138 2231
Size: 84 216 353 340 252 267 28 93
Cuts at: 2231 2431 3263 3695 4048 4710 598
Size: 200 832 432 353 662 1326
Fragments arranged by size:
1326 832 662 432 353 353 340 267
252 216 200 93 84 28
EcoO109I rG'GnC_Cy
Cuts at: 624 640 789 2064 2065 2577 624
Size: 16 149 1275 1 512 3485
Fragments arranged by size:
3485 1275 512 149 16 1
EcoRI G'AATT_C
Cuts at: 1483 2442 1483
Size: 959 4479
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2406 3457 2406
Size: 1051 4387
FspI TGC'GCA
Cuts at: 1279 3219 1279
Size: 1940 3498
HaeII r_GCGC'y
Cuts at: 374 1995 2872 3120 4350 5121 374
Size: 1621 877 248 1230 771 691
Fragments arranged by size:
1621 1230 877 771 691 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 112 144 747 779 1224 1256 3280 3310 3312
Size: 32 603 32 445 32 2024 30 2
Cuts at: 3312 3342 112
Size: 30 2208
Fragments arranged by size:
2208 2024 603 445 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2477 2477
Size: 5438
HindIII A'AGCT_T
Cuts at: 2383 2383
Size: 5438
KasI G'GCGC_C
Cuts at: 2868 3116 2868
Size: 248 5190
KpnI G_GTAC'C
Cuts at: 2458 2458
Size: 5438
MluI A'CGCG_T
Cuts at: 4871 4871
Size: 5438
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 629 4197 4381 629
Size: 3568 184 1686
Fragments arranged by size:
3568 1686 184
MscI TGG'CCA
Cuts at: 3199 3199
Size: 5438
MslI CAynn'nnrTG
Cuts at: 1816 1879 3427 3554 1816
Size: 63 1548 127 3700
Fragments arranged by size:
3700 1548 127 63
MspA1I CmG'CkG
Cuts at: 1897 2549 2863 3223 4007 4252 1897
Size: 652 314 360 784 245 3083
Fragments arranged by size:
3083 784 652 360 314 245
NaeI GCC'GGC
Cuts at: 3619 3619
Size: 5438
NarI GG'CG_CC
Cuts at: 2869 3117 2869
Size: 248 5190
NcoI C'CATG_G
Cuts at: 107 1244 1811 107
Size: 1137 567 3734
Fragments arranged by size:
3734 1137 567
NdeI CA'TA_TG
Cuts at: 1107 1107
Size: 5438
NgoMIV G'CCGG_C
Cuts at: 3617 3617
Size: 5438
NspI r_CATG'y
Cuts at: 621 949 2056 2491 2991 4594 621
Size: 328 1107 435 500 1603 1465
Fragments arranged by size:
1603 1465 1107 500 435 328
PasI CC'CwG_GG
Cuts at: 237 237
Size: 5438
PciI A'CATG_T
Cuts at: 4590 4590
Size: 5438
PflMI CCAn_nnn'nTGG
Cuts at: 519 636 2013 519
Size: 117 1377 3944
Fragments arranged by size:
3944 1377 117
PmeI GTTT'AAAC
Cuts at: 2437 2437
Size: 5438
PmlI CAC'GTG
Cuts at: 2271 2271
Size: 5438
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 655 687 3884 3916 655
Size: 32 3197 32 2177
Fragments arranged by size:
3197 2177 32 32
PpuMI rG'GwC_Cy
Cuts at: 624 624
Size: 5438
PspOMI G'GGCC_C
Cuts at: 639 2064 639
Size: 1425 4013
PstI C_TGCA'G
Cuts at: 2051 2104 2485 3170 2051
Size: 53 381 685 4319
Fragments arranged by size:
4319 685 381 53
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5438
PvuII CAG'CTG
Cuts at: 1897 2863 3223 1897
Size: 966 360 4112
Fragments arranged by size:
4112 966 360
RsrII CG'GwC_CG
Cuts at: 3633 3633
Size: 5438
SacI G_AGCT'C
Cuts at: 1154 1202 2452 1154
Size: 48 1250 4140
Fragments arranged by size:
4140 1250 48
SalI G'TCGA_C
Cuts at: 2475 2475
Size: 5438
SapI GCTCTTCn'nnn_
Cuts at: 139 1506 3461 3671 139
Size: 1367 1955 210 1906
Fragments arranged by size:
1955 1906 1367 210
SbfI CC_TGCA'GG
Cuts at: 2485 2485
Size: 5438
ScaI AGT'ACT
Cuts at: 4955 4955
Size: 5438
SfcI C'TryA_G
Cuts at: 33 1352 2047 2100 2481 3166 4134 4325 33
Size: 1319 695 53 381 685 968 191 1146
Fragments arranged by size:
1319 1146 968 695 685 381 191 53
SfoI GGC'GCC
Cuts at: 2870 3118 2870
Size: 248 5190
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5438
SmaI CCC'GGG
Cuts at: 1720 2460 1720
Size: 740 4698
SmlI C'TyrA_G
Cuts at: 178 379 793 2593 3508 4222 4484 178
Size: 201 414 1800 915 714 262 1132
Fragments arranged by size:
1800 1132 915 714 414 262 201
SnaBI TAC'GTA
Cuts at: 901 901
Size: 5438
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5438
SphI G_CATG'C
Cuts at: 2491 2991 2491
Size: 500 4938
StuI AGG'CCT
Cuts at: 2134 2134
Size: 5438
StyI C'CwwG_G
Cuts at: 107 401 980 1244 1478 1811 1868 2572 2926
Size: 294 579 264 234 333 57 704 354
Cuts at: 2926 107
Size: 2619
Fragments arranged by size:
2619 704 579 354 333 294 264 234
57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 436 1950 2136 3021 3154 3613 3822 436
Size: 1514 186 885 133 459 209 2052
Fragments arranged by size:
2052 1514 885 459 209 186 133
TatI w'GTAC_w
Cuts at: 4953 4953
Size: 5438
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1387 1419 1718 1750 1836 1868 4955 4987 1387
Size: 32 299 32 86 32 3087 32 1838
Fragments arranged by size:
3087 1838 299 86 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 759 3235 759
Size: 2476 2962
XbaI T'CTAG_A
Cuts at: 117 1764 2469 117
Size: 1647 705 3086
Fragments arranged by size:
3086 1647 705
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 417 1065 1818 2933 417
Size: 648 753 1115 2922
Fragments arranged by size:
2922 1115 753 648
XmaI C'CCGG_G
Cuts at: 1718 2458 1718
Size: 740 4698
XmnI GAAnn'nnTTC
Cuts at: 670 670
Size: 5438
ZraI GAC'GTC
Cuts at: 1533 1533
Size: 5438
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AgeI AleI AlfI AscI AvrII BaeI BbvCI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BspEI BsrGI
ClaI EcoNI EcoRV FseI FspAI HpaI MfeI NheI
NotI NruI NsiI PacI PfoI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI
SwaI XhoI