Restriction map for
pF1KB5066
(Circular) (Six-base) MAPSORT of: pF1KB5066.seq Check: 3509 from: 1 to: 4838
pF1KB5066 4838 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 296 1225 296
Size: 929 3909
AccI GT'mk_AC
Cuts at: 709 1876 709
Size: 1167 3671
Acc65I G'GTAC_C
Cuts at: 1854 1854
Size: 4838
AclI AA'CG_TT
Cuts at: 4678 4678
Size: 4838
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 341 470 972 1308 2663 3095 3448 4110
Size: 187 129 502 336 1355 432 353 662
Cuts at: 4110 154
Size: 882
Fragments arranged by size:
1355 882 662 502 432 353 336 187
129
AflIII A'CryG_T
Cuts at: 2190 3990 4271 2190
Size: 1800 281 2757
Fragments arranged by size:
2757 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 281 281
Size: 4838
AlwNI CAG_nnn'CTG
Cuts at: 549 806 1398 2143 3581 549
Size: 257 592 745 1438 1806
Fragments arranged by size:
1806 1438 745 592 257
ApaLI G'TGCA_C
Cuts at: 928 1765 3676 928
Size: 837 1911 2090
Fragments arranged by size:
2090 1911 837
ApoI r'AATT_y
Cuts at: 586 1842 4191 4447 586
Size: 1256 2349 256 977
Fragments arranged by size:
2349 1256 977 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4838
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4838
AvaI C'yCGr_G
Cuts at: 115 1623 1858 115
Size: 1508 235 3095
Fragments arranged by size:
3095 1508 235
BamHI G'GATC_C
Cuts at: 248 1863 248
Size: 1615 3223
BanI G'GyrC_C
Cuts at: 1559 1854 2268 2516 2551 1559
Size: 295 414 248 35 3846
Fragments arranged by size:
3846 414 295 248 35
BanII G_rGCy'C
Cuts at: 1546 1678 1719 1852 2882 1546
Size: 132 41 133 1030 3502
Fragments arranged by size:
3502 1030 133 132 41
BbeI G_GCGC'C
Cuts at: 2272 2520 2272
Size: 248 4590
BciVI GTATCCnnnnn_n'
Cuts at: 2736 3792 4838 2736
Size: 1056 1046 2736
Fragments arranged by size:
2736 1056 1046
BclI T'GATC_A
Cuts at: 2358 2358
Size: 4838
BglI GCCn_nnn'nGGC
Cuts at: 1596 1596
Size: 4838
BglII A'GATC_T
Cuts at: 686 2353 4390 686
Size: 1667 2037 1134
Fragments arranged by size:
2037 1667 1134
BlpI GC'TnA_GC
Cuts at: 1950 1950
Size: 4838
Bme1580I G_kGCm'C
Cuts at: 932 1769 2463 2556 3680 932
Size: 837 694 93 1124 2090
Fragments arranged by size:
2090 1124 837 694 93
BmgBI CAC'GTC
Cuts at: 264 264
Size: 4838
BmrI ACTGGGnnnn_n'
Cuts at: 1124 1208 2050 2131 2466 4610 1124
Size: 84 842 81 335 2144 1352
Fragments arranged by size:
2144 1352 842 335 84 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3205 3205
Size: 4838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 358 732 1052 1650 292
Size: 66 374 320 598 3480
Fragments arranged by size:
3480 598 374 320 66
Bpu10I CC'TnA_GC
Cuts at: 305 305
Size: 4838
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 580 2014 2893 3607 3905 541
Size: 39 1434 879 714 298 1474
Fragments arranged by size:
1474 1434 879 714 298 39
BsaI GGTCTCn'nnnn_
Cuts at: 268 4542 268
Size: 4274 564
BsaAI yAC'GTr
Cuts at: 2191 2191
Size: 4838
BsaBI GATnn'nnATC
Cuts at: 6 2352 4389 6
Size: 2346 2037 455
Fragments arranged by size:
2346 2037 455
BsaHI Gr'CG_yC
Cuts at: 1414 2269 2517 3219 4715 1414
Size: 855 248 702 1496 1537
Fragments arranged by size:
1537 1496 855 702 248
BsaWI w'CCGG_w
Cuts at: 2027 2548 3226 3637 3784 4058 2027
Size: 521 678 411 147 274 2807
Fragments arranged by size:
2807 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 277 307 483 513 691 721 1150 1180 1648
Size: 30 176 30 178 30 429 30 468
Cuts at: 1648 1678 277
Size: 30 3437
Fragments arranged by size:
3437 468 429 178 176 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 469 990 1158 1300 1597 469
Size: 521 168 142 297 3710
Fragments arranged by size:
3710 521 297 168 142
BseYI C'CCAG_C
Cuts at: 332 1346 2263 3021 3686 4094 332
Size: 1014 917 758 665 408 1076
Fragments arranged by size:
1076 1014 917 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 883 1249 883
Size: 366 4472
BsiEI CG_ry'CG
Cuts at: 104 2380 2426 3656 104
Size: 2276 46 1230 1286
Fragments arranged by size:
2276 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 932 1546 1678 1769 1852 2630 2820 3680 932
Size: 614 132 91 83 778 190 860 2090
Fragments arranged by size:
2090 860 778 614 190 132 91 83
Bsp1286I G_dGCh'C
Cuts at: 932 1546 1678 1719 1769 1852 2463 2556 2630
Size: 614 132 41 50 83 611 93 74
Cuts at: 2630 2820 2882 3680 932
Size: 190 62 798 2090
Fragments arranged by size:
2090 798 614 611 190 132 93 83
74 62 50 41
BspHI T'CATG_A
Cuts at: 3270 3270
Size: 4838
BspMI ACCTGCnnnn'nnnn_
Cuts at: 296 1225 1888 2102 2404 2785 296
Size: 929 663 214 302 381 2349
Fragments arranged by size:
2349 929 663 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3130 3184 4668 4833 50
Size: 3080 54 1484 165 55
Fragments arranged by size:
3080 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2750 2750
Size: 4838
BsrFI r'CCGG_y
Cuts at: 156 1604 2836 3017 156
Size: 1448 1232 181 1977
Fragments arranged by size:
1977 1448 1232 181
BssSI C'ACGA_G
Cuts at: 3109 3817 3109
Size: 708 4130
BstAPI GCAn_nnn'nTGC
Cuts at: 2215 2215
Size: 4838
BstBI TT'CG_AA
Cuts at: 3199 3199
Size: 4838
BstEII G'GTnAC_C
Cuts at: 283 283
Size: 4838
BstYI r'GATC_y
Cuts at: 1 248 686 1863 2330 2353 2688 2934 3240
Size: 247 438 1177 467 23 335 246 306
Cuts at: 3240 3252 3338 4390 1
Size: 12 86 1052 449
Fragments arranged by size:
1177 1052 467 449 438 335 306 247
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 710 710
Size: 4838
BtgI C'CryG_G
Cuts at: 107 1593 107
Size: 1486 3352
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2788 2969 3132 2788
Size: 181 163 4494
Fragments arranged by size:
4494 181 163
BtsI GCAGTG_nn'
Cuts at: 1227 1308 2081 1227
Size: 81 773 3984
Fragments arranged by size:
3984 773 81
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 957 992 957
Size: 35 4803
DraI TTT'AAA
Cuts at: 1837 1837
Size: 4838
DrdI GACnn_nn'nnGTC
Cuts at: 2544 3888 2544
Size: 1344 3494
EaeI y'GGCC_r
Cuts at: 2423 2597 2988 3015 4360 2423
Size: 174 391 27 1345 2901
Fragments arranged by size:
2901 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2423 2423
Size: 4838
EarI CTCTTCn'nnn_
Cuts at: 431 446 1778 2861 3071 431
Size: 15 1332 1083 210 2198
Fragments arranged by size:
2198 1332 1083 210 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1700 3046 3788 3934 4648 1700
Size: 1346 742 146 714 1890
Fragments arranged by size:
1890 1346 742 714 146
EcoICRI GAG'CTC
Cuts at: 1544 1676 1850 1544
Size: 132 174 4532
Fragments arranged by size:
4532 174 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 292 341 358 470 732 972 1052 1308
Size: 138 49 17 112 262 240 80 256
Cuts at: 1308 1650 2663 3095 3448 4110 154
Size: 342 1013 432 353 662 882
Fragments arranged by size:
1013 882 662 432 353 342 262 256
240 138 112 80 49 17
EcoNI CCTnn'n_nnAGG
Cuts at: 1626 1626
Size: 4838
EcoO109I rG'GnC_Cy
Cuts at: 301 441 1151 1292 1977 301
Size: 140 710 141 685 3162
Fragments arranged by size:
3162 710 685 141 140
EcoRI G'AATT_C
Cuts at: 1842 1842
Size: 4838
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2857 2857
Size: 4838
FspI TGC'GCA
Cuts at: 2619 2619
Size: 4838
HaeII r_GCGC'y
Cuts at: 2272 2520 3750 4521 2272
Size: 248 1230 771 2589
Fragments arranged by size:
2589 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 267 299 394 426 445 477 483 515 531
Size: 32 95 32 19 32 6 32 16
Cuts at: 531 563 1404 1436 2680 2710 2712 2742 267
Size: 32 841 32 1244 30 2 30 2363
Fragments arranged by size:
2363 1244 841 95 32 32 32 32
32 32 30 30 19 16 6 2
HincII GTy'rAC
Cuts at: 1877 1877
Size: 4838
KasI G'GCGC_C
Cuts at: 2268 2516 2268
Size: 248 4590
KpnI G_GTAC'C
Cuts at: 1858 1858
Size: 4838
MluI A'CGCG_T
Cuts at: 4271 4271
Size: 4838
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 563 3597 3781 563
Size: 3034 184 1620
Fragments arranged by size:
3034 1620 184
MscI TGG'CCA
Cuts at: 2599 2599
Size: 4838
MslI CAynn'nnrTG
Cuts at: 2827 2954 2827
Size: 127 4711
MspA1I CmG'CkG
Cuts at: 231 336 1356 1949 2263 2623 3407 3652 231
Size: 105 1020 593 314 360 784 245 1417
Fragments arranged by size:
1417 1020 784 593 360 314 245 105
NaeI GCC'GGC
Cuts at: 158 1606 3019 158
Size: 1448 1413 1977
Fragments arranged by size:
1977 1448 1413
NarI GG'CG_CC
Cuts at: 2269 2517 2269
Size: 248 4590
NcoI C'CATG_G
Cuts at: 107 1593 107
Size: 1486 3352
NgoMIV G'CCGG_C
Cuts at: 156 1604 3017 156
Size: 1448 1413 1977
Fragments arranged by size:
1977 1448 1413
NspI r_CATG'y
Cuts at: 1891 2391 3994 1891
Size: 500 1603 2735
Fragments arranged by size:
2735 1603 500
PasI CC'CwG_GG
Cuts at: 381 1173 1449 381
Size: 792 276 3770
Fragments arranged by size:
3770 792 276
PciI A'CATG_T
Cuts at: 3990 3990
Size: 4838
PflMI CCAn_nnn'nTGG
Cuts at: 549 549
Size: 4838
PfoI T'CCnGG_A
Cuts at: 188 188
Size: 4838
PmeI GTTT'AAAC
Cuts at: 1837 1837
Size: 4838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3284 3316 3284
Size: 32 4806
PpuMI rG'GwC_Cy
Cuts at: 301 301
Size: 4838
PspXI vC'TCGA_Gb
Cuts at: 1623 1623
Size: 4838
PstI C_TGCA'G
Cuts at: 1222 1683 1885 2570 1222
Size: 461 202 685 3490
Fragments arranged by size:
3490 685 461 202
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4838
PvuII CAG'CTG
Cuts at: 231 336 2263 2623 231
Size: 105 1927 360 2446
Fragments arranged by size:
2446 1927 360 105
RsrII CG'GwC_CG
Cuts at: 3033 3033
Size: 4838
SacI G_AGCT'C
Cuts at: 1546 1678 1852 1546
Size: 132 174 4532
Fragments arranged by size:
4532 174 132
SalI G'TCGA_C
Cuts at: 1875 1875
Size: 4838
SapI GCTCTTCn'nnn_
Cuts at: 1778 2861 3071 1778
Size: 1083 210 3545
Fragments arranged by size:
3545 1083 210
SbfI CC_TGCA'GG
Cuts at: 1885 1885
Size: 4838
ScaI AGT'ACT
Cuts at: 4355 4355
Size: 4838
SexAI A'CCwGG_T
Cuts at: 1640 1640
Size: 4838
SfcI C'TryA_G
Cuts at: 33 1218 1679 1881 2566 3534 3725 33
Size: 1185 461 202 685 968 191 1146
Fragments arranged by size:
1185 1146 968 685 461 202 191
SfoI GGC'GCC
Cuts at: 2270 2518 2270
Size: 248 4590
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4838
SgrAI Cr'CCGG_yG
Cuts at: 156 156
Size: 4838
SmaI CCC'GGG
Cuts at: 1860 1860
Size: 4838
SmlI C'TyrA_G
Cuts at: 556 595 1623 1993 2908 3622 3884 556
Size: 39 1028 370 915 714 262 1510
Fragments arranged by size:
1510 1028 915 714 370 262 39
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4838
SphI G_CATG'C
Cuts at: 1891 2391 1891
Size: 500 4338
StyI C'CwwG_G
Cuts at: 107 1593 1972 2326 107
Size: 1486 379 354 2619
Fragments arranged by size:
2619 1486 379 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 348 364 394 1786 2421 2554 3013 3222 348
Size: 16 30 1392 635 133 459 209 1964
Fragments arranged by size:
1964 1392 635 459 209 133 30 16
TatI w'GTAC_w
Cuts at: 883 1081 4353 883
Size: 198 3272 1368
Fragments arranged by size:
3272 1368 198
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 277 309 802 834 1321 1353 1648 1680 4355
Size: 32 493 32 487 32 295 32 2675
Cuts at: 4355 4387 277
Size: 32 728
Fragments arranged by size:
2675 728 493 487 295 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2635 2635
Size: 4838
XbaI T'CTAG_A
Cuts at: 691 1869 691
Size: 1178 3660
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2333 2333
Size: 4838
XhoI C'TCGA_G
Cuts at: 1623 1623
Size: 4838
XmaI C'CCGG_G
Cuts at: 1858 1858
Size: 4838
XmnI GAAnn'nnTTC
Cuts at: 985 1069 1237 1321 1531 985
Size: 84 168 84 210 4292
Fragments arranged by size:
4292 210 168 84 84
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI
BsiWI BsmI BsmBI BspEI BsrGI BssHII BstXI Bsu36I
ClaI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspOMI PsrI SacII SanDI SfiI SnaBI SrfI
SspI StuI SwaI ZraI