Restriction map for
pF1KB5078
(Circular) (Six-base) MAPSORT of: pF1KB5078.seq Check: 9826 from: 1 to: 5684
pF1KB5078 5684 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 153 1734 2514 153
Size: 1581 780 3323
Fragments arranged by size:
3323 1581 780
AccI GT'mk_AC
Cuts at: 440 2722 440
Size: 2282 3402
Acc65I G'GTAC_C
Cuts at: 2700 2700
Size: 5684
AclI AA'CG_TT
Cuts at: 5524 5524
Size: 5684
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 916 1039 2138 3509 3941 4294 4956 916
Size: 123 1099 1371 432 353 662 1644
Fragments arranged by size:
1644 1371 1099 662 432 353 123
AfeI AGC'GCT
Cuts at: 1153 1849 1153
Size: 696 4988
AflIII A'CryG_T
Cuts at: 260 1701 3036 4836 5117 260
Size: 1441 1335 1800 281 827
Fragments arranged by size:
1800 1441 1335 827 281
AleI CACnn'nnGTG
Cuts at: 628 1939 628
Size: 1311 4373
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1597 1629 1597
Size: 32 5652
AlwNI CAG_nnn'CTG
Cuts at: 766 1779 2445 2506 2625 2989 4427 766
Size: 1013 666 61 119 364 1438 2023
Fragments arranged by size:
2023 1438 1013 666 364 119 61
ApaLI G'TGCA_C
Cuts at: 1017 4522 1017
Size: 3505 2179
ApoI r'AATT_y
Cuts at: 1288 2688 5037 5293 1288
Size: 1400 2349 256 1679
Fragments arranged by size:
2349 1679 1400 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5684
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5684
AvaI C'yCGr_G
Cuts at: 123 2704 123
Size: 2581 3103
BamHI G'GATC_C
Cuts at: 2709 2709
Size: 5684
BanI G'GyrC_C
Cuts at: 174 602 1344 2112 2374 2700 3114 3362 3397
Size: 428 742 768 262 326 414 248 35
Cuts at: 3397 174
Size: 2461
Fragments arranged by size:
2461 768 742 428 414 326 262 248
35
BanII G_rGCy'C
Cuts at: 2048 2438 2698 3728 2048
Size: 390 260 1030 4004
Fragments arranged by size:
4004 1030 390 260
BbeI G_GCGC'C
Cuts at: 1348 3118 3366 1348
Size: 1770 248 3666
Fragments arranged by size:
3666 1770 248
BbsI GAAGACnn'nnnn_
Cuts at: 1681 2027 1681
Size: 346 5338
BbvCI CC'TCA_GC
Cuts at: 1691 1691
Size: 5684
BciVI GTATCCnnnnn_n'
Cuts at: 1076 2646 3582 4638 5684 1076
Size: 1570 936 1056 1046 1076
Fragments arranged by size:
1570 1076 1056 1046 936
BclI T'GATC_A
Cuts at: 3204 3204
Size: 5684
BglI GCCn_nnn'nGGC
Cuts at: 188 2363 188
Size: 2175 3509
BglII A'GATC_T
Cuts at: 356 3199 5236 356
Size: 2843 2037 804
Fragments arranged by size:
2843 2037 804
BlpI GC'TnA_GC
Cuts at: 2796 2796
Size: 5684
Bme1580I G_kGCm'C
Cuts at: 1021 2377 2541 3309 3402 4526 1021
Size: 1356 164 768 93 1124 2179
Fragments arranged by size:
2179 1356 1124 768 164 93
BmgBI CAC'GTC
Cuts at: 261 261
Size: 5684
BmrI ACTGGGnnnn_n'
Cuts at: 2896 2977 3312 5456 2896
Size: 81 335 2144 3124
Fragments arranged by size:
3124 2144 335 81
BmtI G_CTAG'C
Cuts at: 508 971 508
Size: 463 5221
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1732 4051 1732
Size: 2319 3365
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1015 1202 2422 1015
Size: 187 1220 4277
Fragments arranged by size:
4277 1220 187
Bpu10I CC'TnA_GC
Cuts at: 1691 1691
Size: 5684
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1045 1486 1609 1780 2860 3739 4453 4751 1045
Size: 441 123 171 1080 879 714 298 1978
Fragments arranged by size:
1978 1080 879 714 441 298 171 123
BsaI GGTCTCn'nnnn_
Cuts at: 495 5388 495
Size: 4893 791
BsaAI yAC'GTr
Cuts at: 2472 3037 2472
Size: 565 5119
BsaBI GATnn'nnATC
Cuts at: 6 1441 1552 3198 5235 6
Size: 1435 111 1646 2037 455
Fragments arranged by size:
2037 1646 1435 455 111
BsaHI Gr'CG_yC
Cuts at: 1345 1678 3115 3363 4065 5561 1345
Size: 333 1437 248 702 1496 1468
Fragments arranged by size:
1496 1468 1437 702 333 248
BsaWI w'CCGG_w
Cuts at: 2873 3394 4072 4483 4630 4904 2873
Size: 521 678 411 147 274 3653
Fragments arranged by size:
3653 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1597 1627 1877 1907 1597
Size: 30 250 30 5374
Fragments arranged by size:
5374 250 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1246 2038 1246
Size: 792 4892
BseYI C'CCAG_C
Cuts at: 675 1144 1905 1966 2292 2296 2499 3109 3867
Size: 469 761 61 326 4 203 610 758
Cuts at: 3867 4532 4940 675
Size: 665 408 1419
Fragments arranged by size:
1419 761 758 665 610 469 408 326
203 61 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1762 2242 2373 2659 1762
Size: 480 131 286 4787
Fragments arranged by size:
4787 480 286 131
BsiEI CG_ry'CG
Cuts at: 104 1368 3226 3272 4502 104
Size: 1264 1858 46 1230 1286
Fragments arranged by size:
1858 1286 1264 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1021 1502 2048 2438 2698 3476 3666 4526 1021
Size: 481 546 390 260 778 190 860 2179
Fragments arranged by size:
2179 860 778 546 481 390 260 190
BsiWI C'GTAC_G
Cuts at: 2472 2472
Size: 5684
BsmI GAATG_Cn'
Cuts at: 530 1260 530
Size: 730 4954
Bsp1286I G_dGCh'C
Cuts at: 1021 1502 2048 2377 2438 2541 2698 3309 3402
Size: 481 546 329 61 103 157 611 93
Cuts at: 3402 3476 3666 3728 4526 1021
Size: 74 190 62 798 2179
Fragments arranged by size:
2179 798 611 546 481 329 190 157
103 93 74 62 61
BspHI T'CATG_A
Cuts at: 4116 4116
Size: 5684
BspMI ACCTGCnnnn'nnnn_
Cuts at: 153 155 1734 2514 2631 2734 2948 3250 3631
Size: 2 1579 780 117 103 214 302 381
Cuts at: 3631 153
Size: 2206
Fragments arranged by size:
2206 1579 780 381 302 214 117 103
2
BsrBI CCG'CTC
Cuts at: 50 638 1216 3976 4030 5514 5679 50
Size: 588 578 2760 54 1484 165 55
Fragments arranged by size:
2760 1484 588 578 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1379 2610 3596 1379
Size: 1231 986 3467
Fragments arranged by size:
3467 1231 986
BsrFI r'CCGG_y
Cuts at: 182 1341 1680 3682 3863 182
Size: 1159 339 2002 181 2003
Fragments arranged by size:
2003 2002 1159 339 181
BssSI C'ACGA_G
Cuts at: 3955 4663 3955
Size: 708 4976
BstAPI GCAn_nnn'nTGC
Cuts at: 1740 3061 1740
Size: 1321 4363
BstBI TT'CG_AA
Cuts at: 4045 4045
Size: 5684
BstXI CCAn_nnnn'nTGG
Cuts at: 1291 1291
Size: 5684
BstYI r'GATC_y
Cuts at: 1 356 554 1210 1272 2441 2709 3176 3199
Size: 355 198 656 62 1169 268 467 23
Cuts at: 3199 3534 3780 4086 4098 4184 5236 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1169 1052 656 467 449 355 335 306
268 246 198 86 62 23 12
Bsu36I CC'TnA_GG
Cuts at: 656 656
Size: 5684
BtgI C'CryG_G
Cuts at: 107 2002 107
Size: 1895 3789
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1443 3634 3815 3978 1443
Size: 2191 181 163 3149
Fragments arranged by size:
3149 2191 181 163
BtsI GCAGTG_nn'
Cuts at: 415 764 1741 1946 2927 415
Size: 349 977 205 981 3172
Fragments arranged by size:
3172 981 977 349 205
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1797 1832 1797
Size: 35 5649
DraI TTT'AAA
Cuts at: 2683 2683
Size: 5684
DraIII CAC_nnn'GTG
Cuts at: 2553 2553
Size: 5684
DrdI GACnn_nn'nnGTC
Cuts at: 3390 4734 3390
Size: 1344 4340
EaeI y'GGCC_r
Cuts at: 224 549 593 1092 1365 2156 2627 3269 3443
Size: 325 44 499 273 791 471 642 174
Cuts at: 3443 3834 3861 5206 224
Size: 391 27 1345 702
Fragments arranged by size:
1345 791 702 642 499 471 391 325
273 174 44 27
EagI C'GGCC_G
Cuts at: 1365 3269 1365
Size: 1904 3780
EarI CTCTTCn'nnn_
Cuts at: 922 1487 2272 3707 3917 922
Size: 565 785 1435 210 2689
Fragments arranged by size:
2689 1435 785 565 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3892 4634 4780 5494 3892
Size: 742 146 714 4082
Fragments arranged by size:
4082 742 714 146
EcoICRI GAG'CTC
Cuts at: 2046 2436 2696 2046
Size: 390 260 5034
Fragments arranged by size:
5034 390 260
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 916 1015 1039 1202 2138 2422 3509 3941 4294
Size: 99 24 163 936 284 1087 432 353
Cuts at: 4294 4956 916
Size: 662 1644
Fragments arranged by size:
1644 1087 936 662 432 353 284 163
99 24
EcoNI CCTnn'n_nnAGG
Cuts at: 160 464 160
Size: 304 5380
EcoO109I rG'GnC_Cy
Cuts at: 118 537 891 2613 2622 2823 118
Size: 419 354 1722 9 201 2979
Fragments arranged by size:
2979 1722 419 354 201 9
EcoRI G'AATT_C
Cuts at: 2688 2688
Size: 5684
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3703 3703
Size: 5684
FspI TGC'GCA
Cuts at: 2662 3465 2662
Size: 803 4881
HaeII r_GCGC'y
Cuts at: 143 1155 1348 1851 3118 3366 4596 5367 143
Size: 1012 193 503 1267 248 1230 771 460
Fragments arranged by size:
1267 1230 1012 771 503 460 248 193
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1755 1787 3526 3556 3558 3588 1755
Size: 32 1739 30 2 30 3851
Fragments arranged by size:
3851 1739 32 30 30 2
HincII GTy'rAC
Cuts at: 312 441 1564 2723 312
Size: 129 1123 1159 3273
Fragments arranged by size:
3273 1159 1123 129
KasI G'GCGC_C
Cuts at: 1344 3114 3362 1344
Size: 1770 248 3666
Fragments arranged by size:
3666 1770 248
KpnI G_GTAC'C
Cuts at: 2704 2704
Size: 5684
MluI A'CGCG_T
Cuts at: 5117 5117
Size: 5684
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1686 1808 4443 4627 1686
Size: 122 2635 184 2743
Fragments arranged by size:
2743 2635 184 122
MscI TGG'CCA
Cuts at: 226 595 1094 2158 2629 3445 226
Size: 369 499 1064 471 816 2465
Fragments arranged by size:
2465 1064 816 499 471 369
MslI CAynn'nnrTG
Cuts at: 628 1939 2396 2570 3673 3800 628
Size: 1311 457 174 1103 127 2512
Fragments arranged by size:
2512 1311 1103 457 174 127
MspA1I CmG'CkG
Cuts at: 133 548 675 993 1521 2296 2457 2795 3109
Size: 415 127 318 528 775 161 338 314
Cuts at: 3109 3469 4253 4498 133
Size: 360 784 245 1319
Fragments arranged by size:
1319 784 775 528 415 360 338 318
314 245 161 127
NaeI GCC'GGC
Cuts at: 184 1343 3865 184
Size: 1159 2522 2003
Fragments arranged by size:
2522 2003 1159
NarI GG'CG_CC
Cuts at: 1345 3115 3363 1345
Size: 1770 248 3666
Fragments arranged by size:
3666 1770 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5684
NgoMIV G'CCGG_C
Cuts at: 182 1341 3863 182
Size: 1159 2522 2003
Fragments arranged by size:
2522 2003 1159
NheI G'CTAG_C
Cuts at: 504 967 504
Size: 463 5221
NspI r_CATG'y
Cuts at: 1413 2737 3237 4840 1413
Size: 1324 500 1603 2257
Fragments arranged by size:
2257 1603 1324 500
PasI CC'CwG_GG
Cuts at: 460 460
Size: 5684
PciI A'CATG_T
Cuts at: 4836 4836
Size: 5684
PflMI CCAn_nnn'nTGG
Cuts at: 500 675 2553 2625 500
Size: 175 1878 72 3559
Fragments arranged by size:
3559 1878 175 72
PfoI T'CCnGG_A
Cuts at: 1283 1283
Size: 5684
PmeI GTTT'AAAC
Cuts at: 2683 2683
Size: 5684
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 682 714 1597 1621 1629 1653 4130 4162 682
Size: 32 883 24 8 24 2477 32 2204
Fragments arranged by size:
2477 2204 883 32 32 24 24 8
PpuMI rG'GwC_Cy
Cuts at: 118 2613 118
Size: 2495 3189
PsiI TTA'TAA
Cuts at: 776 776
Size: 5684
PstI C_TGCA'G
Cuts at: 161 1649 2731 3416 161
Size: 1488 1082 685 2429
Fragments arranged by size:
2429 1488 1082 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5684
PvuII CAG'CTG
Cuts at: 133 993 1521 2296 2457 3109 3469 133
Size: 860 528 775 161 652 360 2348
Fragments arranged by size:
2348 860 775 652 528 360 161
RsrII CG'GwC_CG
Cuts at: 3879 3879
Size: 5684
SacI G_AGCT'C
Cuts at: 2048 2438 2698 2048
Size: 390 260 5034
Fragments arranged by size:
5034 390 260
SalI G'TCGA_C
Cuts at: 439 2721 439
Size: 2282 3402
SanDI GG'GwC_CC
Cuts at: 118 2613 118
Size: 2495 3189
SapI GCTCTTCn'nnn_
Cuts at: 3707 3917 3707
Size: 210 5474
SbfI CC_TGCA'GG
Cuts at: 2731 2731
Size: 5684
ScaI AGT'ACT
Cuts at: 5201 5201
Size: 5684
SexAI A'CCwGG_T
Cuts at: 2549 2549
Size: 5684
SfcI C'TryA_G
Cuts at: 33 157 1645 2727 3412 4380 4571 33
Size: 124 1488 1082 685 968 191 1146
Fragments arranged by size:
1488 1146 1082 968 685 191 124
SfoI GGC'GCC
Cuts at: 1346 3116 3364 1346
Size: 1770 248 3666
Fragments arranged by size:
3666 1770 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5684
SgrAI Cr'CCGG_yG
Cuts at: 1680 1680
Size: 5684
SmaI CCC'GGG
Cuts at: 2706 2706
Size: 5684
SmlI C'TyrA_G
Cuts at: 1060 1501 1624 1795 2839 3754 4468 4730 1060
Size: 441 123 171 1044 915 714 262 2014
Fragments arranged by size:
2014 1044 915 714 441 262 171 123
SnaBI TAC'GTA
Cuts at: 2472 2472
Size: 5684
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5684
SphI G_CATG'C
Cuts at: 2737 3237 2737
Size: 500 5184
StyI C'CwwG_G
Cuts at: 107 566 878 1535 2818 3172 107
Size: 459 312 657 1283 354 2619
Fragments arranged by size:
2619 1283 657 459 354 312
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 925 1186 1404 2338 2342 3267 3400 3859 4068
Size: 261 218 934 4 925 133 459 209
Cuts at: 4068 925
Size: 2541
Fragments arranged by size:
2541 934 925 459 261 218 209 133
4
TatI w'GTAC_w
Cuts at: 2669 5199 2669
Size: 2530 3154
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 595 627 995 1027 5201 5233 595
Size: 32 368 32 4174 32 1046
Fragments arranged by size:
4174 1046 368 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1779 3481 1779
Size: 1702 3982
XbaI T'CTAG_A
Cuts at: 2715 2715
Size: 5684
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 603 3179 603
Size: 2576 3108
XmaI C'CCGG_G
Cuts at: 2704 2704
Size: 5684
XmnI GAAnn'nnTTC
Cuts at: 1423 1423
Size: 5684
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AlfI ApaI AscI AvrII
BaeI BcgI BfrBI BsmBI BspEI BsrGI BssHII BstEII
BstZ17I ClaI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NotI NruI NsiI PacI PmlI PshAI PspOMI
PspXI PsrI SacII SfiI SrfI SspI StuI SwaI
XhoI ZraI