Restriction map for
pF1KB5081
(Circular) (Six-base) MAPSORT of: pF1KB5081.seq Check: 2132 from: 1 to: 4736
pF1KB5081 4736 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 1457 1496 1774 1457
Size: 39 278 4419
Fragments arranged by size:
4419 278 39
Acc65I G'GTAC_C
Cuts at: 1752 1752
Size: 4736
AclI AA'CG_TT
Cuts at: 4576 4576
Size: 4736
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 878 2561 2993 3740 4008 878
Size: 1683 432 747 268 1606
Fragments arranged by size:
1683 1606 747 432 268
AfeI AGC'GCT
Cuts at: 361 361
Size: 4736
AflIII A'CryG_T
Cuts at: 2088 3192 4169 2088
Size: 1104 977 2655
Fragments arranged by size:
2655 1104 977
AgeI A'CCGG_T
Cuts at: 1499 3124 1499
Size: 1625 3111
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 661 661
Size: 4736
AlwNI CAG_nnn'CTG
Cuts at: 1594 2041 3608 1594
Size: 447 1567 2722
Fragments arranged by size:
2722 1567 447
ApaI G_GGCC'C
Cuts at: 355 1688 355
Size: 1333 3403
ApaLI G'TGCA_C
Cuts at: 3506 3506
Size: 4736
ApoI r'AATT_y
Cuts at: 1740 4089 4345 1740
Size: 2349 256 2131
Fragments arranged by size:
2349 2131 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4736
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4736
AvaI C'yCGr_G
Cuts at: 125 230 381 1756 125
Size: 105 151 1375 3105
Fragments arranged by size:
3105 1375 151 105
BamHI G'GATC_C
Cuts at: 1629 1761 1629
Size: 132 4604
BanI G'GyrC_C
Cuts at: 451 490 574 765 1252 1273 1752 2166 2414
Size: 39 84 191 487 21 479 414 248
Cuts at: 2414 2449 451
Size: 35 2738
Fragments arranged by size:
2738 487 479 414 248 191 84 39
35 21
BanII G_rGCy'C
Cuts at: 143 158 355 1061 1688 1750 2780 143
Size: 15 197 706 627 62 1030 2099
Fragments arranged by size:
2099 1030 706 627 197 62 15
BbeI G_GCGC'C
Cuts at: 455 494 578 1256 2170 2418 455
Size: 39 84 678 914 248 2773
Fragments arranged by size:
2773 914 678 248 84 39
BbsI GAAGACnn'nnnn_
Cuts at: 369 369
Size: 4736
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 426 460 426
Size: 34 4702
BciVI GTATCCnnnnn_n'
Cuts at: 2634 3395 4736 2634
Size: 761 1341 2634
Fragments arranged by size:
2634 1341 761
BclI T'GATC_A
Cuts at: 413 2256 413
Size: 1843 2893
BglI GCCn_nnn'nGGC
Cuts at: 112 295 304 488 774 112
Size: 183 9 184 286 4074
Fragments arranged by size:
4074 286 184 183 9
BglII A'GATC_T
Cuts at: 2251 4288 2251
Size: 2037 2699
BlpI GC'TnA_GC
Cuts at: 1318 1848 1318
Size: 530 4206
Bme1580I G_kGCm'C
Cuts at: 355 770 1688 2361 2454 3510 355
Size: 415 918 673 93 1056 1581
Fragments arranged by size:
1581 1056 918 673 415 93
BmrI ACTGGGnnnn_n'
Cuts at: 325 1948 2029 2364 4508 325
Size: 1623 81 335 2144 553
Fragments arranged by size:
2144 1623 553 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1432 3103 1432
Size: 1671 3065
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1427 1427
Size: 4736
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 384 1912 2791 3283 3581 384
Size: 1528 879 492 298 1539
Fragments arranged by size:
1539 1528 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 921 4440 921
Size: 3519 1217
BsaAI yAC'GTr
Cuts at: 1563 2089 1563
Size: 526 4210
BsaBI GATnn'nnATC
Cuts at: 6 415 796 2250 4287 6
Size: 409 381 1454 2037 455
Fragments arranged by size:
2037 1454 455 409 381
BsaHI Gr'CG_yC
Cuts at: 452 491 532 575 1132 1253 2167 2415 3117
Size: 39 41 43 557 121 914 248 702
Cuts at: 3117 4613 452
Size: 1496 575
Fragments arranged by size:
1496 914 702 575 557 248 121 43
41 39
BsaWI w'CCGG_w
Cuts at: 748 1499 1925 2446 3124 3398 3545 3956 748
Size: 751 426 521 678 274 147 411 1528
Fragments arranged by size:
1528 751 678 521 426 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1450 1480 1450
Size: 30 4706
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 113 1216 1540 1657 113
Size: 1103 324 117 3192
Fragments arranged by size:
3192 1103 324 117
BseYI C'CCAG_C
Cuts at: 309 634 849 1038 2161 2919 3496 3992 309
Size: 325 215 189 1123 758 577 496 1053
Fragments arranged by size:
1123 1053 758 577 496 325 215 189
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 456 456
Size: 4736
BsiEI CG_ry'CG
Cuts at: 104 483 1457 1500 1706 2278 2324 3532 104
Size: 379 974 43 206 572 46 1208 1308
Fragments arranged by size:
1308 1208 974 572 379 206 46 43
BsiHKAI G_wGCw'C
Cuts at: 650 1750 2528 2718 3510 650
Size: 1100 778 190 792 1876
Fragments arranged by size:
1876 1100 792 778 190
BsiWI C'GTAC_G
Cuts at: 1570 1570
Size: 4736
Bsp1286I G_dGCh'C
Cuts at: 143 158 355 650 770 1061 1688 1750 2361
Size: 15 197 295 120 291 627 62 611
Cuts at: 2361 2454 2528 2718 2780 3510 143
Size: 93 74 190 62 730 1369
Fragments arranged by size:
1369 730 627 611 295 291 197 190
120 93 74 62 62 15
BspEI T'CCGG_A
Cuts at: 3956 3956
Size: 4736
BspHI T'CATG_A
Cuts at: 3912 3912
Size: 4736
BspMI ACCTGCnnnn'nnnn_
Cuts at: 272 1786 2000 2302 2683 272
Size: 1514 214 302 381 2325
Fragments arranged by size:
2325 1514 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3028 3082 4566 4731 50
Size: 2978 54 1484 165 55
Fragments arranged by size:
2978 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1307 2648 1307
Size: 1341 3395
BsrFI r'CCGG_y
Cuts at: 298 1499 2734 2915 3124 298
Size: 1201 1235 181 209 1910
Fragments arranged by size:
1910 1235 1201 209 181
BssHII G'CGCG_C
Cuts at: 473 473
Size: 4736
BssSI C'ACGA_G
Cuts at: 3007 3365 3007
Size: 358 4378
BstAPI GCAn_nnn'nTGC
Cuts at: 1696 2113 1696
Size: 417 4319
BstBI TT'CG_AA
Cuts at: 3097 3097
Size: 4736
BstEII G'GTnAC_C
Cuts at: 516 516
Size: 4736
BstXI CCAn_nnnn'nTGG
Cuts at: 528 912 528
Size: 384 4352
BstYI r'GATC_y
Cuts at: 1 663 1629 1761 2228 2251 2586 2832 3844
Size: 662 966 132 467 23 335 246 1012
Cuts at: 3844 3930 3942 4288 1
Size: 86 12 346 449
Fragments arranged by size:
1012 966 662 467 449 346 335 246
132 86 23 12
BtgI C'CryG_G
Cuts at: 107 290 602 611 770 905 1053 1700 107
Size: 183 312 9 159 135 148 647 3143
Fragments arranged by size:
3143 647 312 183 159 148 135 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2686 2867 3030 2686
Size: 181 163 4392
Fragments arranged by size:
4392 181 163
BtsI GCAGTG_nn'
Cuts at: 624 1142 1307 1979 624
Size: 518 165 672 3381
Fragments arranged by size:
3381 672 518 165
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1131 1166 1131
Size: 35 4701
DraI TTT'AAA
Cuts at: 1735 1735
Size: 4736
DrdI GACnn_nn'nnGTC
Cuts at: 1492 2442 3300 1492
Size: 950 858 2928
Fragments arranged by size:
2928 950 858
EaeI y'GGCC_r
Cuts at: 113 287 296 480 1139 1295 1367 1424 2321
Size: 174 9 184 659 156 72 57 897
Cuts at: 2321 2495 2886 2913 4258 113
Size: 174 391 27 1345 591
Fragments arranged by size:
1345 897 659 591 391 184 174 174
156 72 57 27 9
EagI C'GGCC_G
Cuts at: 480 2321 480
Size: 1841 2895
EarI CTCTTCn'nnn_
Cuts at: 728 1093 1164 1288 1606 2759 2969 728
Size: 365 71 124 318 1153 210 2495
Fragments arranged by size:
2495 1153 365 318 210 124 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 166 573 1158 2944 3254 3400 4546 166
Size: 407 585 1786 310 146 1146 356
Fragments arranged by size:
1786 1146 585 407 356 310 146
EcoICRI GAG'CTC
Cuts at: 1748 1748
Size: 4736
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 878 1427 2561 2993 3740 4008 878
Size: 549 1134 432 747 268 1606
Fragments arranged by size:
1606 1134 747 549 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1406 1406
Size: 4736
EcoO109I rG'GnC_Cy
Cuts at: 171 853 1154 1684 1875 171
Size: 682 301 530 191 3032
Fragments arranged by size:
3032 682 530 301 191
EcoRI G'AATT_C
Cuts at: 1740 1740
Size: 4736
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2755 2755
Size: 4736
FspI TGC'GCA
Cuts at: 2517 2517
Size: 4736
HaeII r_GCGC'y
Cuts at: 181 310 363 371 455 494 502 541 559
Size: 129 53 8 84 39 8 39 18
Cuts at: 559 578 841 1256 2170 2418 3440 4419 181
Size: 19 263 415 914 248 1022 979 498
Fragments arranged by size:
1022 979 914 498 415 263 248 129
84 53 39 39 19 18 8 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1450 1482 1705 1737 2578 2608 2610 2640 1450
Size: 32 223 32 841 30 2 30 3546
Fragments arranged by size:
3546 841 223 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1263 1446 1458 1497 1775 1263
Size: 183 12 39 278 4224
Fragments arranged by size:
4224 278 183 39 12
KasI G'GCGC_C
Cuts at: 451 490 574 1252 2166 2414 451
Size: 39 84 678 914 248 2773
Fragments arranged by size:
2773 914 678 248 84 39
KpnI G_GTAC'C
Cuts at: 1756 1756
Size: 4736
MluI A'CGCG_T
Cuts at: 4169 4169
Size: 4736
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 713 3407 3591 713
Size: 2694 184 1858
Fragments arranged by size:
2694 1858 184
MscI TGG'CCA
Cuts at: 1141 1426 2497 1141
Size: 285 1071 3380
Fragments arranged by size:
3380 1071 285
MslI CAynn'nnrTG
Cuts at: 2725 2852 2725
Size: 127 4609
MspA1I CmG'CkG
Cuts at: 148 248 426 613 1137 1182 1393 1635 1847
Size: 100 178 187 524 45 211 242 212
Cuts at: 1847 2161 2521 3534 3779 148
Size: 314 360 1013 245 1105
Fragments arranged by size:
1105 1013 524 360 314 245 242 212
211 187 178 100 45
NaeI GCC'GGC
Cuts at: 300 2917 300
Size: 2617 2119
NarI GG'CG_CC
Cuts at: 452 491 575 1253 2167 2415 452
Size: 39 84 678 914 248 2773
Fragments arranged by size:
2773 914 678 248 84 39
NcoI C'CATG_G
Cuts at: 107 905 1053 107
Size: 798 148 3790
Fragments arranged by size:
3790 798 148
NgoMIV G'CCGG_C
Cuts at: 298 2915 298
Size: 2617 2119
NspI r_CATG'y
Cuts at: 1789 2289 3196 1789
Size: 500 907 3329
Fragments arranged by size:
3329 907 500
PciI A'CATG_T
Cuts at: 3192 3192
Size: 4736
PflMI CCAn_nnn'nTGG
Cuts at: 1293 1293
Size: 4736
PfoI T'CCnGG_A
Cuts at: 659 919 659
Size: 260 4476
PmeI GTTT'AAAC
Cuts at: 1735 1735
Size: 4736
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3875 3907 3875
Size: 32 4704
PpuMI rG'GwC_Cy
Cuts at: 1154 1154
Size: 4736
PspOMI G'GGCC_C
Cuts at: 351 1684 351
Size: 1333 3403
PstI C_TGCA'G
Cuts at: 569 1783 2468 569
Size: 1214 685 2837
Fragments arranged by size:
2837 1214 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4736
PvuII CAG'CTG
Cuts at: 2161 2521 2161
Size: 360 4376
RsrII CG'GwC_CG
Cuts at: 2931 2931
Size: 4736
SacI G_AGCT'C
Cuts at: 1750 1750
Size: 4736
SacII CC_GC'GG
Cuts at: 614 614
Size: 4736
SalI G'TCGA_C
Cuts at: 1456 1495 1773 1456
Size: 39 278 4419
Fragments arranged by size:
4419 278 39
SapI GCTCTTCn'nnn_
Cuts at: 1288 2759 2969 1288
Size: 1471 210 3055
Fragments arranged by size:
3055 1471 210
SbfI CC_TGCA'GG
Cuts at: 1783 1783
Size: 4736
ScaI AGT'ACT
Cuts at: 4253 4253
Size: 4736
SexAI A'CCwGG_T
Cuts at: 236 692 236
Size: 456 4280
SfcI C'TryA_G
Cuts at: 33 565 1779 2464 3457 3648 33
Size: 532 1214 685 993 191 1121
Fragments arranged by size:
1214 1121 993 685 532 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 295 295
Size: 4736
SfoI GGC'GCC
Cuts at: 453 492 576 1254 2168 2416 453
Size: 39 84 678 914 248 2773
Fragments arranged by size:
2773 914 678 248 84 39
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4736
SmaI CCC'GGG
Cuts at: 232 383 1758 232
Size: 151 1375 3210
Fragments arranged by size:
3210 1375 151
SmlI C'TyrA_G
Cuts at: 125 363 1891 2806 3298 3560 125
Size: 238 1528 915 492 262 1301
Fragments arranged by size:
1528 1301 915 492 262 238
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4736
SphI G_CATG'C
Cuts at: 1789 2289 1789
Size: 500 4236
StyI C'CwwG_G
Cuts at: 107 134 198 905 1011 1053 1331 1870 2224
Size: 27 64 707 106 42 278 539 354
Cuts at: 2224 107
Size: 2619
Fragments arranged by size:
2619 707 539 354 278 106 64 42
27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2319 2452 2911 3120 2319
Size: 133 459 209 3935
Fragments arranged by size:
3935 459 209 133
TatI w'GTAC_w
Cuts at: 1019 4251 1019
Size: 3232 1504
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 305 337 743 775 4253 4285 305
Size: 32 406 32 3478 32 756
Fragments arranged by size:
3478 756 406 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2533 2533
Size: 4736
XbaI T'CTAG_A
Cuts at: 1767 1767
Size: 4736
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 171 909 1263 2231 171
Size: 738 354 968 2676
Fragments arranged by size:
2676 968 738 354
XhoI C'TCGA_G
Cuts at: 125 125
Size: 4736
XmaI C'CCGG_G
Cuts at: 230 381 1756 230
Size: 151 1375 3210
Fragments arranged by size:
3210 1375 151
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsmI BsmBI
BsrGI BstZ17I Bsu36I ClaI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PspXI PsrI SanDI
SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI