Restriction map for
pF1KB5089
(Circular) (Six-base) MAPSORT of: pF1KB5089.seq Check: 4057 from: 1 to: 6815
pF1KB5089 6815 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 585 1522 3107 585
Size: 937 1585 4293
Fragments arranged by size:
4293 1585 937
AccI GT'mk_AC
Cuts at: 3853 3853
Size: 6815
Acc65I G'GTAC_C
Cuts at: 2198 3831 2198
Size: 1633 5182
AclI AA'CG_TT
Cuts at: 6655 6655
Size: 6815
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 655 4640 5072 5819 6087 607
Size: 48 3985 432 747 268 1335
Fragments arranged by size:
3985 1335 747 432 268 48
AflIII A'CryG_T
Cuts at: 1199 2357 4167 5271 6248 1199
Size: 1158 1810 1104 977 1766
Fragments arranged by size:
1810 1766 1158 1104 977
AgeI A'CCGG_T
Cuts at: 5203 5203
Size: 6815
AhdI GACnn_n'nnGTC
Cuts at: 215 978 2793 215
Size: 763 1815 4237
Fragments arranged by size:
4237 1815 763
AleI CACnn'nnGTG
Cuts at: 160 1264 2065 160
Size: 1104 801 4910
Fragments arranged by size:
4910 1104 801
AlwNI CAG_nnn'CTG
Cuts at: 963 1074 1482 2319 2805 3231 3300 3579 4120
Size: 111 408 837 486 426 69 279 541
Cuts at: 4120 5687 963
Size: 1567 2091
Fragments arranged by size:
2091 1567 837 541 486 426 408 279
111 69
ApaI G_GGCC'C
Cuts at: 277 2727 277
Size: 2450 4365
ApaLI G'TGCA_C
Cuts at: 5585 5585
Size: 6815
ApoI r'AATT_y
Cuts at: 694 2518 3819 6168 6424 694
Size: 1824 1301 2349 256 1085
Fragments arranged by size:
2349 1824 1301 1085 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6815
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6815
AvaI C'yCGr_G
Cuts at: 876 3748 3835 876
Size: 2872 87 3856
Fragments arranged by size:
3856 2872 87
AvrII C'CTAG_G
Cuts at: 3141 3141
Size: 6815
BamHI G'GATC_C
Cuts at: 614 2534 3840 614
Size: 1920 1306 3589
Fragments arranged by size:
3589 1920 1306
BanI G'GyrC_C
Cuts at: 115 808 1266 1517 2059 2111 2198 2528 3119
Size: 693 458 251 542 52 87 330 591
Cuts at: 3119 3136 3761 3831 4245 4493 4528 115
Size: 17 625 70 414 248 35 2402
Fragments arranged by size:
2402 693 625 591 542 458 414 330
251 248 87 70 52 35 17
BanII G_rGCy'C
Cuts at: 277 950 1600 1616 2005 2447 2727 2978 3605
Size: 673 650 16 389 442 280 251 627
Cuts at: 3605 3829 4859 277
Size: 224 1030 2233
Fragments arranged by size:
2233 1030 673 650 627 442 389 280
251 224 16
BbeI G_GCGC'C
Cuts at: 119 812 4249 4497 119
Size: 693 3437 248 2437
Fragments arranged by size:
3437 2437 693 248
BbvCI CC'TCA_GC
Cuts at: 2902 3110 2902
Size: 208 6607
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1582 1616 1843 1877 1582
Size: 34 227 34 6520
Fragments arranged by size:
6520 227 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1504 2557 4713 5474 6815 1504
Size: 1053 2156 761 1341 1504
Fragments arranged by size:
2156 1504 1341 1053 761
BclI T'GATC_A
Cuts at: 2684 4335 2684
Size: 1651 5164
BglI GCCn_nnn'nGGC
Cuts at: 909 3622 3683 3699 909
Size: 2713 61 16 4025
Fragments arranged by size:
4025 2713 61 16
BglII A'GATC_T
Cuts at: 4330 6367 4330
Size: 2037 4778
BlpI GC'TnA_GC
Cuts at: 2038 3927 2038
Size: 1889 4926
Bme1580I G_kGCm'C
Cuts at: 277 1843 2062 2531 2727 3141 3764 4440 4533
Size: 1566 219 469 196 414 623 676 93
Cuts at: 4533 5589 277
Size: 1056 1503
Fragments arranged by size:
1566 1503 1056 676 623 469 414 219
196 93
BmgBI CAC'GTC
Cuts at: 1200 1281 2703 2780 1200
Size: 81 1422 77 5235
Fragments arranged by size:
5235 1422 81 77
BmrI ACTGGGnnnn_n'
Cuts at: 2787 4027 4108 4443 6587 2787
Size: 1240 81 335 2144 3015
Fragments arranged by size:
3015 2144 1240 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 228 1635 2777 3314 5182 228
Size: 1407 1142 537 1868 1861
Fragments arranged by size:
1868 1861 1407 1142 537
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 424 820 856 985 1306 1588 1723 3256
Size: 226 396 36 129 321 282 135 1533
Cuts at: 3256 3544 198
Size: 288 3469
Fragments arranged by size:
3469 1533 396 321 288 282 226 135
129 36
Bpu10I CC'TnA_GC
Cuts at: 1588 1663 2762 2902 3098 3110 3310 1588
Size: 75 1099 140 196 12 200 5093
Fragments arranged by size:
5093 1099 200 196 140 75 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1708 2191 3991 4870 5362 5660 1708
Size: 483 1800 879 492 298 2863
Fragments arranged by size:
2863 1800 879 492 483 298
BsaI GGTCTCn'nnnn_
Cuts at: 160 202 965 1640 1966 2816 3017 6519 160
Size: 42 763 675 326 850 201 3502 456
Fragments arranged by size:
3502 850 763 675 456 326 201 42
BsaAI yAC'GTr
Cuts at: 2346 4168 2346
Size: 1822 4993
BsaBI GATnn'nnATC
Cuts at: 6 4329 6366 6
Size: 4323 2037 455
Fragments arranged by size:
4323 2037 455
BsaHI Gr'CG_yC
Cuts at: 116 128 809 4246 4494 5196 6692 116
Size: 12 681 3437 248 702 1496 239
Fragments arranged by size:
3437 1496 702 681 248 239 12
BsaWI w'CCGG_w
Cuts at: 701 2531 2560 3005 4004 4525 5203 5477 5624
Size: 1830 29 445 999 521 678 274 147
Cuts at: 5624 6035 701
Size: 411 1481
Fragments arranged by size:
1830 1481 999 678 521 445 411 274
147 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1381 1411 1381
Size: 30 6785
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 421 520 1030 1086 1237 1608 1690 1795
Size: 201 99 510 56 151 371 82 105
Cuts at: 1795 1813 1885 2064 2521 3541 3613 3808 220
Size: 18 72 179 457 1020 72 195 3227
Fragments arranged by size:
3227 1020 510 457 371 201 195 179
151 105 99 82 72 72 56 18
BseYI C'CCAG_C
Cuts at: 119 1067 1132 1468 1525 1629 1639 1696 2090
Size: 948 65 336 57 104 10 57 394
Cuts at: 2090 2805 2836 3269 3562 3617 3681 3702 4240
Size: 715 31 433 293 55 64 21 538
Cuts at: 4240 4998 5575 6071 119
Size: 758 577 496 863
Fragments arranged by size:
948 863 758 715 577 538 496 433
394 336 293 104 65 64 57 57
55 31 21 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 3244 496
Size: 2748 4067
BsiEI CG_ry'CG
Cuts at: 104 778 1216 2230 4357 4403 5611 104
Size: 674 438 1014 2127 46 1208 1308
Fragments arranged by size:
2127 1308 1208 1014 674 438 46
BsiHKAI G_wGCw'C
Cuts at: 1057 1600 2978 3829 4607 4797 5589 1057
Size: 543 1378 851 778 190 792 2283
Fragments arranged by size:
2283 1378 851 792 778 543 190
BsmI GAATG_Cn'
Cuts at: 1866 1866
Size: 6815
BsmBI CGTCTCn'nnnn_
Cuts at: 1403 2770 1403
Size: 1367 5448
Bsp1286I G_dGCh'C
Cuts at: 277 950 1057 1600 1616 1843 2005 2062 2447
Size: 673 107 543 16 227 162 57 385
Cuts at: 2447 2531 2727 2978 3141 3605 3764 3829 4440
Size: 84 196 251 163 464 159 65 611
Cuts at: 4440 4533 4607 4797 4859 5589 277
Size: 93 74 190 62 730 1503
Fragments arranged by size:
1503 730 673 611 543 464 385 251
227 196 190 163 162 159 107 93
84 74 65 62 57 16
BspEI T'CCGG_A
Cuts at: 2560 3005 6035 2560
Size: 445 3030 3340
Fragments arranged by size:
3340 3030 445
BspHI T'CATG_A
Cuts at: 5991 5991
Size: 6815
BspMI ACCTGCnnnn'nnnn_
Cuts at: 585 1522 1770 2210 3035 3107 3564 3865 4079
Size: 937 248 440 825 72 457 301 214
Cuts at: 4079 4381 4762 585
Size: 302 381 2638
Fragments arranged by size:
2638 937 825 457 440 381 302 301
248 214 72
BsrBI CCG'CTC
Cuts at: 50 750 1397 2558 5107 5161 6645 6810 50
Size: 700 647 1161 2549 54 1484 165 55
Fragments arranged by size:
2549 1484 1161 700 647 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1292 1674 2492 4727 1292
Size: 382 818 2235 3380
Fragments arranged by size:
3380 2235 818 382
BsrFI r'CCGG_y
Cuts at: 332 2613 2861 3011 3122 4813 4994 5203 332
Size: 2281 248 150 111 1691 181 209 1944
Fragments arranged by size:
2281 1944 1691 248 209 181 150 111
BssSI C'ACGA_G
Cuts at: 5086 5444 5086
Size: 358 6457
BstAPI GCAn_nnn'nTGC
Cuts at: 3231 3579 4192 3231
Size: 348 613 5854
Fragments arranged by size:
5854 613 348
BstBI TT'CG_AA
Cuts at: 5176 5176
Size: 6815
BstEII G'GTnAC_C
Cuts at: 228 228
Size: 6815
BstXI CCAn_nnnn'nTGG
Cuts at: 162 1983 2844 2967 3612 162
Size: 1821 861 123 645 3365
Fragments arranged by size:
3365 1821 861 645 123
BstYI r'GATC_y
Cuts at: 1 362 614 791 1047 2534 3395 3665 3840
Size: 361 252 177 256 1487 861 270 175
Cuts at: 3840 4307 4330 4665 4911 5923 6009 6021 6367
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6367 1
Size: 449
Fragments arranged by size:
1487 1012 861 467 449 361 346 335
270 256 252 246 177 175 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 1220 1220
Size: 6815
BtgI C'CryG_G
Cuts at: 107 158 983 1242 1982 2006 107
Size: 51 825 259 740 24 4916
Fragments arranged by size:
4916 825 740 259 51 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2167 2916 4765 4946 5109 2167
Size: 749 1849 181 163 3873
Fragments arranged by size:
3873 1849 749 181 163
BtsI GCAGTG_nn'
Cuts at: 1115 4058 1115
Size: 2943 3872
DraI TTT'AAA
Cuts at: 3814 3814
Size: 6815
DraIII CAC_nnn'GTG
Cuts at: 1078 1078
Size: 6815
DrdI GACnn_nn'nnGTC
Cuts at: 426 4521 5379 426
Size: 4095 858 1862
Fragments arranged by size:
4095 1862 858
EaeI y'GGCC_r
Cuts at: 470 775 1213 1604 2227 2279 2735 2769 3130
Size: 305 438 391 623 52 456 34 361
Cuts at: 3130 4400 4574 4965 4992 6337 470
Size: 1270 174 391 27 1345 948
Fragments arranged by size:
1345 1270 948 623 456 438 391 391
361 305 174 52 34 27
EagI C'GGCC_G
Cuts at: 775 1213 2227 4400 775
Size: 438 1014 2173 3190
Fragments arranged by size:
3190 2173 1014 438
EarI CTCTTCn'nnn_
Cuts at: 1790 2101 2435 3590 4838 5048 1790
Size: 311 334 1155 1248 210 3557
Fragments arranged by size:
3557 1248 1155 334 311 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3478 3658 5023 5333 5479 6625 3478
Size: 180 1365 310 146 1146 3668
Fragments arranged by size:
3668 1365 1146 310 180 146
EcoICRI GAG'CTC
Cuts at: 1598 2976 3827 1598
Size: 1378 851 4586
Fragments arranged by size:
4586 1378 851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 424 607 655 820 856 985 1306 1588
Size: 226 183 48 165 36 129 321 282
Cuts at: 1588 1723 3256 3544 4640 5072 5819 6087 198
Size: 135 1533 288 1096 432 747 268 926
Fragments arranged by size:
1533 1096 926 747 432 321 288 282
268 226 183 165 135 129 48 36
EcoNI CCTnn'n_nnAGG
Cuts at: 349 2947 3097 3292 349
Size: 2598 150 195 3872
Fragments arranged by size:
3872 2598 195 150
EcoO109I rG'GnC_Cy
Cuts at: 274 354 388 1136 1479 1731 1744 2679 2723
Size: 80 34 748 343 252 13 935 44
Cuts at: 2723 3576 3954 274
Size: 853 378 3135
Fragments arranged by size:
3135 935 853 748 378 343 252 80
44 34 13
EcoRI G'AATT_C
Cuts at: 3819 3819
Size: 6815
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4834 4834
Size: 6815
FspI TGC'GCA
Cuts at: 568 3040 4596 568
Size: 2472 1556 2787
Fragments arranged by size:
2787 2472 1556
HaeII r_GCGC'y
Cuts at: 119 812 1550 3414 3776 4249 4497 5519 6498
Size: 693 738 1864 362 473 248 1022 979
Cuts at: 6498 119
Size: 436
Fragments arranged by size:
1864 1022 979 738 693 473 436 362
248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 201 233 964 996 1861 1893 1945 1977 2779
Size: 32 731 32 865 32 52 32 802
Cuts at: 2779 2811 3468 3500 4657 4687 4689 4719 201
Size: 32 657 32 1157 30 2 30 2297
Fragments arranged by size:
2297 1157 865 802 731 657 52 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1890 3507 3854 1890
Size: 1617 347 4851
Fragments arranged by size:
4851 1617 347
KasI G'GCGC_C
Cuts at: 115 808 4245 4493 115
Size: 693 3437 248 2437
Fragments arranged by size:
3437 2437 693 248
KpnI G_GTAC'C
Cuts at: 2202 3835 2202
Size: 1633 5182
MluI A'CGCG_T
Cuts at: 6248 6248
Size: 6815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 125 199 592 2102 2348 5486 5670 125
Size: 74 393 1510 246 3138 184 1270
Fragments arranged by size:
3138 1510 1270 393 246 184 74
MscI TGG'CCA
Cuts at: 2281 2771 4576 2281
Size: 490 1805 4520
Fragments arranged by size:
4520 1805 490
MslI CAynn'nnrTG
Cuts at: 160 286 472 1060 1170 1264 2065 2344 4804
Size: 126 186 588 110 94 801 279 2460
Cuts at: 4804 4931 160
Size: 127 2044
Fragments arranged by size:
2460 2044 801 588 279 186 127 126
110 94
MspA1I CmG'CkG
Cuts at: 1128 1184 1446 1629 1652 2195 2604 2733 2805
Size: 56 262 183 23 543 409 129 72
Cuts at: 2805 2967 3054 3154 3273 3306 3543 3588 3621
Size: 162 87 100 119 33 237 45 33
Cuts at: 3621 3645 3758 3926 4240 4600 5613 5858 1128
Size: 24 113 168 314 360 1013 245 2085
Fragments arranged by size:
2085 1013 543 409 360 314 262 245
237 183 168 162 129 119 113 100
87 72 56 45 33 33 24 23
NaeI GCC'GGC
Cuts at: 2615 2863 3013 3124 4996 2615
Size: 248 150 111 1872 4434
Fragments arranged by size:
4434 1872 248 150 111
NarI GG'CG_CC
Cuts at: 116 809 4246 4494 116
Size: 693 3437 248 2437
Fragments arranged by size:
3437 2437 693 248
NcoI C'CATG_G
Cuts at: 107 983 1982 2006 107
Size: 876 999 24 4916
Fragments arranged by size:
4916 999 876 24
NgoMIV G'CCGG_C
Cuts at: 2613 2861 3011 3122 4994 2613
Size: 248 150 111 1872 4434
Fragments arranged by size:
4434 1872 248 150 111
NspI r_CATG'y
Cuts at: 2088 2361 3868 4368 5275 2088
Size: 273 1507 500 907 3628
Fragments arranged by size:
3628 1507 907 500 273
PasI CC'CwG_GG
Cuts at: 2911 2911
Size: 6815
PciI A'CATG_T
Cuts at: 2357 5271 2357
Size: 2914 3901
PflMI CCAn_nnn'nTGG
Cuts at: 497 497
Size: 6815
PfoI T'CCnGG_A
Cuts at: 365 550 3398 365
Size: 185 2848 3782
Fragments arranged by size:
3782 2848 185
PmeI GTTT'AAAC
Cuts at: 3814 3814
Size: 6815
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5954 5986 5954
Size: 32 6783
PpuMI rG'GwC_Cy
Cuts at: 354 388 1136 1479 3576 354
Size: 34 748 343 2097 3593
Fragments arranged by size:
3593 2097 748 343 34
PspOMI G'GGCC_C
Cuts at: 273 2723 273
Size: 2450 4365
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3190 3222 3190
Size: 32 6783
PstI C_TGCA'G
Cuts at: 143 596 3305 3392 3862 4547 143
Size: 453 2709 87 470 685 2411
Fragments arranged by size:
2709 2411 685 470 453 87
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6815
PvuII CAG'CTG
Cuts at: 1128 1629 1652 2733 2805 3588 3621 4240 4600
Size: 501 23 1081 72 783 33 619 360
Cuts at: 4600 1128
Size: 3343
Fragments arranged by size:
3343 1081 783 619 501 360 72 33
23
RsrII CG'GwC_CG
Cuts at: 5010 5010
Size: 6815
SacI G_AGCT'C
Cuts at: 1600 2978 3829 1600
Size: 1378 851 4586
Fragments arranged by size:
4586 1378 851
SalI G'TCGA_C
Cuts at: 3852 3852
Size: 6815
SanDI GG'GwC_CC
Cuts at: 388 388
Size: 6815
SapI GCTCTTCn'nnn_
Cuts at: 2101 2435 4838 5048 2101
Size: 334 2403 210 3868
Fragments arranged by size:
3868 2403 334 210
SbfI CC_TGCA'GG
Cuts at: 3862 3862
Size: 6815
ScaI AGT'ACT
Cuts at: 1390 6332 1390
Size: 4942 1873
SexAI A'CCwGG_T
Cuts at: 224 224
Size: 6815
SfcI C'TryA_G
Cuts at: 33 139 592 1829 3301 3388 3858 4543 5536
Size: 106 453 1237 1472 87 470 685 993
Cuts at: 5536 5727 33
Size: 191 1121
Fragments arranged by size:
1472 1237 1121 993 685 470 453 191
106 87
SfiI GGCCn_nnn'nGGCC
Cuts at: 3622 3622
Size: 6815
SfoI GGC'GCC
Cuts at: 117 810 4247 4495 117
Size: 693 3437 248 2437
Fragments arranged by size:
3437 2437 693 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6815
SmaI CCC'GGG
Cuts at: 3750 3837 3750
Size: 87 6728
SmlI C'TyrA_G
Cuts at: 1687 2206 3970 4885 5377 5639 1687
Size: 519 1764 915 492 262 2863
Fragments arranged by size:
2863 1764 915 519 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6815
SphI G_CATG'C
Cuts at: 3868 4368 3868
Size: 500 6315
StuI AGG'CCT
Cuts at: 1661 1661
Size: 6815
StyI C'CwwG_G
Cuts at: 107 350 491 734 812 983 1976 1982 2006
Size: 243 141 243 78 171 993 6 24
Cuts at: 2006 2054 2459 3141 3949 4303 107
Size: 48 405 682 808 354 2619
Fragments arranged by size:
2619 993 808 682 405 354 243 243
171 141 78 48 24 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1711 2806 4398 4531 4990 5199 1711
Size: 1095 1592 133 459 209 3327
Fragments arranged by size:
3327 1592 1095 459 209 133
TatI w'GTAC_w
Cuts at: 263 1388 2180 6330 263
Size: 1125 792 4150 748
Fragments arranged by size:
4150 1125 792 748
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6332 6364 6332
Size: 32 6783
Tth111I GACn'n_nGTC
Cuts at: 1140 4612 1140
Size: 3472 3343
XbaI T'CTAG_A
Cuts at: 3846 3846
Size: 6815
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 222 285 2004 2013 3519 4310 222
Size: 63 1719 9 1506 791 2727
Fragments arranged by size:
2727 1719 1506 791 63 9
XmaI C'CCGG_G
Cuts at: 3748 3835 3748
Size: 87 6728
XmnI GAAnn'nnTTC
Cuts at: 559 640 2419 559
Size: 81 1779 4955
Fragments arranged by size:
4955 1779 81
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI BaeI BbsI
BfrBI BmtI BsiWI BsrGI BssHII BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspXI
SacII SgrAI SnaBI SrfI SspI SwaI XhoI ZraI