Restriction map for
pF1KB5090
(Circular) (Six-base) MAPSORT of: pF1KB5090.seq Check: 5923 from: 1 to: 5420
pF1KB5090 5420 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 2458 2458
Size: 5420
Acc65I G'GTAC_C
Cuts at: 146 2436 146
Size: 2290 3130
AclI AA'CG_TT
Cuts at: 5260 5260
Size: 5420
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 535 916 2213 2242 3245 3677 4030 4692
Size: 174 381 1297 29 1003 432 353 662
Cuts at: 4692 361
Size: 1089
Fragments arranged by size:
1297 1089 1003 662 432 381 353 174
29
AflIII A'CryG_T
Cuts at: 2048 2772 4572 4853 2048
Size: 724 1800 281 2615
Fragments arranged by size:
2615 1800 724 281
AleI CACnn'nnGTG
Cuts at: 271 1282 1975 271
Size: 1011 693 3716
Fragments arranged by size:
3716 1011 693
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 869 1114 1942 869
Size: 245 828 4347
Fragments arranged by size:
4347 828 245
AlwNI CAG_nnn'CTG
Cuts at: 207 469 537 714 2040 2209 2725 4163 207
Size: 262 68 177 1326 169 516 1438 1464
Fragments arranged by size:
1464 1438 1326 516 262 177 169 68
ApaLI G'TGCA_C
Cuts at: 4258 4258
Size: 5420
ApoI r'AATT_y
Cuts at: 1168 1255 1327 1750 2424 4773 5029 1168
Size: 87 72 423 674 2349 256 1559
Fragments arranged by size:
2349 1559 674 423 256 87 72
AseI AT'TA_AT
Cuts at: 20 20
Size: 5420
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5420
AvaI C'yCGr_G
Cuts at: 2440 2440
Size: 5420
BamHI G'GATC_C
Cuts at: 1246 2445 1246
Size: 1199 4221
BanI G'GyrC_C
Cuts at: 146 428 1918 2436 2850 3098 3133 146
Size: 282 1490 518 414 248 35 2433
Fragments arranged by size:
2433 1490 518 414 282 248 35
BanII G_rGCy'C
Cuts at: 2434 3464 2434
Size: 1030 4390
BbeI G_GCGC'C
Cuts at: 2854 3102 2854
Size: 248 5172
BbsI GAAGACnn'nnnn_
Cuts at: 2230 2230
Size: 5420
BbvCI CC'TCA_GC
Cuts at: 2343 2343
Size: 5420
BciVI GTATCCnnnnn_n'
Cuts at: 3318 4374 5420 3318
Size: 1056 1046 3318
Fragments arranged by size:
3318 1056 1046
BclI T'GATC_A
Cuts at: 1421 2940 1421
Size: 1519 3901
BfrBI ATG'CAT
Cuts at: 1101 1101
Size: 5420
BglI GCCn_nnn'nGGC
Cuts at: 472 472
Size: 5420
BglII A'GATC_T
Cuts at: 666 2935 4972 666
Size: 2269 2037 1114
Fragments arranged by size:
2269 2037 1114
BlpI GC'TnA_GC
Cuts at: 2532 2532
Size: 5420
Bme1580I G_kGCm'C
Cuts at: 1273 3045 3138 4262 1273
Size: 1772 93 1124 2431
Fragments arranged by size:
2431 1772 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2632 2713 3048 5192 2632
Size: 81 335 2144 2860
Fragments arranged by size:
2860 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1494 2406 3787 1494
Size: 912 1381 3127
Fragments arranged by size:
3127 1381 912
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 559 595 979 1042 1898 172
Size: 387 36 384 63 856 3694
Fragments arranged by size:
3694 856 387 384 63 36
Bpu10I CC'TnA_GC
Cuts at: 1630 2343 1630
Size: 713 4707
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 628 1441 1920 2596 3475 4189 4487 412
Size: 216 813 479 676 879 714 298 1345
Fragments arranged by size:
1345 879 813 714 676 479 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 1426 1465 5124 1426
Size: 39 3659 1722
Fragments arranged by size:
3659 1722 39
BsaAI yAC'GTr
Cuts at: 798 2773 798
Size: 1975 3445
BsaBI GATnn'nnATC
Cuts at: 6 2934 4971 6
Size: 2928 2037 455
Fragments arranged by size:
2928 2037 455
BsaHI Gr'CG_yC
Cuts at: 1133 1531 1961 2851 3099 3801 5297 1133
Size: 398 430 890 248 702 1496 1256
Fragments arranged by size:
1496 1256 890 702 430 398 248
BsaWI w'CCGG_w
Cuts at: 560 2609 3130 3808 4219 4366 4640 560
Size: 2049 521 678 411 147 274 1340
Fragments arranged by size:
2049 1340 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1896 1926 1896
Size: 30 5390
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 835 976 1039 1423 1635 2161 835
Size: 141 63 384 212 526 4094
Fragments arranged by size:
4094 526 384 212 141 63
BseYI C'CCAG_C
Cuts at: 476 2845 3603 4268 4676 476
Size: 2369 758 665 408 1220
Fragments arranged by size:
2369 1220 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 820 1399 592
Size: 228 579 4613
Fragments arranged by size:
4613 579 228
BsiEI CG_ry'CG
Cuts at: 104 1015 2962 3008 4238 104
Size: 911 1947 46 1230 1286
Fragments arranged by size:
1947 1286 1230 911 46
BsiHKAI G_wGCw'C
Cuts at: 1906 2434 3212 3402 4262 1906
Size: 528 778 190 860 3064
Fragments arranged by size:
3064 860 778 528 190
BsmI GAATG_Cn'
Cuts at: 2152 2152
Size: 5420
Bsp1286I G_dGCh'C
Cuts at: 1273 1906 2434 3045 3138 3212 3402 3464 4262
Size: 633 528 611 93 74 190 62 798
Cuts at: 4262 1273
Size: 2431
Fragments arranged by size:
2431 798 633 611 528 190 93 74
62
BspEI T'CCGG_A
Cuts at: 560 560
Size: 5420
BspHI T'CATG_A
Cuts at: 3852 3852
Size: 5420
BspMI ACCTGCnnnn'nnnn_
Cuts at: 374 2263 2282 2470 2684 2986 3367 374
Size: 1889 19 188 214 302 381 2427
Fragments arranged by size:
2427 1889 381 302 214 188 19
BsrBI CCG'CTC
Cuts at: 50 1080 1884 1890 3712 3766 5250 5415 50
Size: 1030 804 6 1822 54 1484 165 55
Fragments arranged by size:
1822 1484 1030 804 165 55 54 6
BsrDI GCAATG_nn'
Cuts at: 3332 3332
Size: 5420
BsrFI r'CCGG_y
Cuts at: 1010 1109 1129 3418 3599 1010
Size: 99 20 2289 181 2831
Fragments arranged by size:
2831 2289 181 99 20
BsrGI T'GTAC_A
Cuts at: 1002 1002
Size: 5420
BssSI C'ACGA_G
Cuts at: 3691 4399 3691
Size: 708 4712
BstAPI GCAn_nnn'nTGC
Cuts at: 469 534 705 2797 469
Size: 65 171 2092 3092
Fragments arranged by size:
3092 2092 171 65
BstBI TT'CG_AA
Cuts at: 3781 3781
Size: 5420
BstXI CCAn_nnnn'nTGG
Cuts at: 1526 2103 1526
Size: 577 4843
BstYI r'GATC_y
Cuts at: 1 576 666 1246 2006 2445 2912 2935 3270
Size: 575 90 580 760 439 467 23 335
Cuts at: 3270 3516 3822 3834 3920 4972 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 760 580 575 467 449 439 335
306 246 90 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1345 1345
Size: 5420
BtgI C'CryG_G
Cuts at: 107 512 2015 2411 107
Size: 405 1503 396 3116
Fragments arranged by size:
3116 1503 405 396
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 802 3370 3551 3714 802
Size: 2568 181 163 2508
Fragments arranged by size:
2568 2508 181 163
BtsI GCAGTG_nn'
Cuts at: 467 728 2663 467
Size: 261 1935 3224
Fragments arranged by size:
3224 1935 261
DraI TTT'AAA
Cuts at: 2419 2419
Size: 5420
DrdI GACnn_nn'nnGTC
Cuts at: 3126 4470 3126
Size: 1344 4076
EaeI y'GGCC_r
Cuts at: 245 1012 1619 2170 3005 3179 3570 3597 4942
Size: 767 607 551 835 174 391 27 1345
Cuts at: 4942 245
Size: 723
Fragments arranged by size:
1345 835 767 723 607 551 391 174
27
EagI C'GGCC_G
Cuts at: 1012 3005 1012
Size: 1993 3427
EarI CTCTTCn'nnn_
Cuts at: 559 832 3443 3653 559
Size: 273 2611 210 2326
Fragments arranged by size:
2611 2326 273 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3628 4370 4516 5230 3628
Size: 742 146 714 3818
Fragments arranged by size:
3818 742 714 146
EcoICRI GAG'CTC
Cuts at: 2432 2432
Size: 5420
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 361 535 559 595 916 979 1042 1898
Size: 189 174 24 36 321 63 63 856
Cuts at: 1898 2213 2242 3245 3677 4030 4692 172
Size: 315 29 1003 432 353 662 900
Fragments arranged by size:
1003 900 856 662 432 353 321 315
189 174 63 63 36 29 24
EcoO109I rG'GnC_Cy
Cuts at: 993 1626 1708 2559 993
Size: 633 82 851 3854
Fragments arranged by size:
3854 851 633 82
EcoRI G'AATT_C
Cuts at: 1327 2424 1327
Size: 1097 4323
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3439 3439
Size: 5420
FspI TGC'GCA
Cuts at: 3201 3201
Size: 5420
HaeII r_GCGC'y
Cuts at: 2210 2854 3102 4332 5103 2210
Size: 644 248 1230 771 2527
Fragments arranged by size:
2527 1230 771 644 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 210 242 372 404 1204 1236 1548 1580 1655
Size: 32 130 32 800 32 312 32 75
Cuts at: 1655 1687 3262 3292 3294 3324 210
Size: 32 1575 30 2 30 2306
Fragments arranged by size:
2306 1575 800 312 130 75 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 943 2387 2459 943
Size: 1444 72 3904
Fragments arranged by size:
3904 1444 72
KasI G'GCGC_C
Cuts at: 2850 3098 2850
Size: 248 5172
KpnI G_GTAC'C
Cuts at: 150 2440 150
Size: 2290 3130
MfeI C'AATT_G
Cuts at: 1380 1598 1380
Size: 218 5202
MluI A'CGCG_T
Cuts at: 4853 4853
Size: 5420
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1328 1526 4179 4363 1328
Size: 198 2653 184 2385
Fragments arranged by size:
2653 2385 198 184
MscI TGG'CCA
Cuts at: 3181 3181
Size: 5420
MslI CAynn'nnrTG
Cuts at: 271 1096 1282 1975 3409 3536 271
Size: 825 186 693 1434 127 2155
Fragments arranged by size:
2155 1434 825 693 186 127
MspA1I CmG'CkG
Cuts at: 714 1680 1994 2040 2531 2845 3205 3989 4234
Size: 966 314 46 491 314 360 784 245
Cuts at: 4234 714
Size: 1900
Fragments arranged by size:
1900 966 784 491 360 314 314 245
46
NaeI GCC'GGC
Cuts at: 3601 3601
Size: 5420
NarI GG'CG_CC
Cuts at: 2851 3099 2851
Size: 248 5172
NcoI C'CATG_G
Cuts at: 107 2411 107
Size: 2304 3116
NdeI CA'TA_TG
Cuts at: 240 240
Size: 5420
NgoMIV G'CCGG_C
Cuts at: 3599 3599
Size: 5420
NsiI A_TGCA'T
Cuts at: 1103 1103
Size: 5420
NspI r_CATG'y
Cuts at: 265 663 1095 1554 2052 2473 2973 4576 265
Size: 398 432 459 498 421 500 1603 1109
Fragments arranged by size:
1603 1109 500 498 459 432 421 398
PciI A'CATG_T
Cuts at: 2048 4572 2048
Size: 2524 2896
PflMI CCAn_nnn'nTGG
Cuts at: 1541 2016 1541
Size: 475 4945
PfoI T'CCnGG_A
Cuts at: 611 1249 611
Size: 638 4782
PmeI GTTT'AAAC
Cuts at: 2419 2419
Size: 5420
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 710 742 3866 3898 710
Size: 32 3124 32 2232
Fragments arranged by size:
3124 2232 32 32
PpuMI rG'GwC_Cy
Cuts at: 993 1626 1708 993
Size: 633 82 4705
Fragments arranged by size:
4705 633 82
PshAI GACnn'nnGTC
Cuts at: 1132 1132
Size: 5420
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1840 1872 1840
Size: 32 5388
PstI C_TGCA'G
Cuts at: 465 1326 2260 2467 3152 465
Size: 861 934 207 685 2733
Fragments arranged by size:
2733 934 861 685 207
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5420
PvuII CAG'CTG
Cuts at: 714 1680 2040 2845 3205 714
Size: 966 360 805 360 2929
Fragments arranged by size:
2929 966 805 360 360
RsrII CG'GwC_CG
Cuts at: 3615 3615
Size: 5420
SacI G_AGCT'C
Cuts at: 2434 2434
Size: 5420
SalI G'TCGA_C
Cuts at: 2457 2457
Size: 5420
SanDI GG'GwC_CC
Cuts at: 993 993
Size: 5420
SapI GCTCTTCn'nnn_
Cuts at: 3443 3653 3443
Size: 210 5210
SbfI CC_TGCA'GG
Cuts at: 2467 2467
Size: 5420
ScaI AGT'ACT
Cuts at: 4937 4937
Size: 5420
SexAI A'CCwGG_T
Cuts at: 1710 1710
Size: 5420
SfcI C'TryA_G
Cuts at: 33 127 171 454 461 1322 2256 2463 3148
Size: 94 44 283 7 861 934 207 685
Cuts at: 3148 4116 4307 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 934 861 685 283 207 191
94 44 7
SfoI GGC'GCC
Cuts at: 2852 3100 2852
Size: 248 5172
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5420
SmaI CCC'GGG
Cuts at: 2442 2442
Size: 5420
SmlI C'TyrA_G
Cuts at: 391 643 1456 1899 2575 3490 4204 4466 391
Size: 252 813 443 676 915 714 262 1345
Fragments arranged by size:
1345 915 813 714 676 443 262 252
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5420
SphI G_CATG'C
Cuts at: 1095 2473 2973 1095
Size: 1378 500 3542
Fragments arranged by size:
3542 1378 500
StuI AGG'CCT
Cuts at: 1454 1454
Size: 5420
StyI C'CwwG_G
Cuts at: 107 214 1449 2141 2411 2554 2908 107
Size: 107 1235 692 270 143 354 2619
Fragments arranged by size:
2619 1235 692 354 270 143 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1450 1965 3003 3136 3595 3804 1450
Size: 515 1038 133 459 209 3066
Fragments arranged by size:
3066 1038 515 459 209 133
TatI w'GTAC_w
Cuts at: 1002 1829 4935 1002
Size: 827 3106 1487
Fragments arranged by size:
3106 1487 827
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1700 1732 1896 1928 4937 4969 1700
Size: 32 164 32 3009 32 2151
Fragments arranged by size:
3009 2151 164 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3217 3217
Size: 5420
XbaI T'CTAG_A
Cuts at: 579 2451 579
Size: 1872 3548
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 510 1604 2915 510
Size: 1094 1311 3015
Fragments arranged by size:
3015 1311 1094
XmaI C'CCGG_G
Cuts at: 2440 2440
Size: 5420
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI ApaI
AscI AvrII BaeI BcgI BmgBI BmtI BsiWI BsmBI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI NheI NotI NruI PacI
PasI PmlI PsiI PspOMI PspXI SacII SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI