Restriction map for
pF1KB5096
(Circular) (Six-base) MAPSORT of: pF1KB5096.seq Check: 8121 from: 1 to: 4859
pF1KB5096 4859 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1897 1897
Size: 4859
Acc65I G'GTAC_C
Cuts at: 1875 1875
Size: 4859
AclI AA'CG_TT
Cuts at: 4699 4699
Size: 4859
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 1600 1776 2684 3116 3469 4131 189
Size: 1411 176 908 432 353 662 917
Fragments arranged by size:
1411 917 908 662 432 353 176
AfeI AGC'GCT
Cuts at: 274 439 1123 274
Size: 165 684 4010
Fragments arranged by size:
4010 684 165
AflIII A'CryG_T
Cuts at: 2211 4011 4292 2211
Size: 1800 281 2778
Fragments arranged by size:
2778 1800 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1111 1143 1111
Size: 32 4827
AlwNI CAG_nnn'CTG
Cuts at: 1578 2164 3602 1578
Size: 586 1438 2835
Fragments arranged by size:
2835 1438 586
ApaI G_GGCC'C
Cuts at: 1055 1055
Size: 4859
ApaLI G'TGCA_C
Cuts at: 820 3697 820
Size: 2877 1982
ApoI r'AATT_y
Cuts at: 909 1240 1561 1863 4212 4468 909
Size: 331 321 302 2349 256 1300
Fragments arranged by size:
2349 1300 331 321 302 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4859
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4859
AvaI C'yCGr_G
Cuts at: 247 847 1879 247
Size: 600 1032 3227
Fragments arranged by size:
3227 1032 600
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 243 276 243
Size: 33 4826
BamHI G'GATC_C
Cuts at: 1481 1884 1481
Size: 403 4456
BanI G'GyrC_C
Cuts at: 163 374 591 879 1875 2289 2537 2572 163
Size: 211 217 288 996 414 248 35 2450
Fragments arranged by size:
2450 996 414 288 248 217 211 35
BanII G_rGCy'C
Cuts at: 848 1055 1433 1873 2903 848
Size: 207 378 440 1030 2804
Fragments arranged by size:
2804 1030 440 378 207
BbeI G_GCGC'C
Cuts at: 378 2293 2541 378
Size: 1915 248 2696
Fragments arranged by size:
2696 1915 248
BbsI GAAGACnn'nnnn_
Cuts at: 1104 1239 1518 1776 1104
Size: 135 279 258 4187
Fragments arranged by size:
4187 279 258 135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 153 187 663 697 153
Size: 34 476 34 4315
Fragments arranged by size:
4315 476 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1156 2757 3813 4859 1156
Size: 1601 1056 1046 1156
Fragments arranged by size:
1601 1156 1056 1046
BclI T'GATC_A
Cuts at: 1679 2379 1679
Size: 700 4159
BglI GCCn_nnn'nGGC
Cuts at: 877 877
Size: 4859
BglII A'GATC_T
Cuts at: 2374 4411 2374
Size: 2037 2822
BlpI GC'TnA_GC
Cuts at: 1971 1971
Size: 4859
Bme1580I G_kGCm'C
Cuts at: 596 824 1055 2484 2577 3701 596
Size: 228 231 1429 93 1124 1754
Fragments arranged by size:
1754 1429 1124 231 228 93
BmrI ACTGGGnnnn_n'
Cuts at: 276 2071 2152 2487 4631 276
Size: 1795 81 335 2144 504
Fragments arranged by size:
2144 1795 504 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3226 3226
Size: 4859
Bpu10I CC'TnA_GC
Cuts at: 1432 1432
Size: 4859
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2035 2914 3628 3926 2035
Size: 879 714 298 2968
Fragments arranged by size:
2968 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 460 4563 460
Size: 4103 756
BsaAI yAC'GTr
Cuts at: 259 2212 259
Size: 1953 2906
BsaBI GATnn'nnATC
Cuts at: 6 2373 4410 6
Size: 2367 2037 455
Fragments arranged by size:
2367 2037 455
BsaHI Gr'CG_yC
Cuts at: 375 1285 1797 2290 2538 3240 4736 375
Size: 910 512 493 248 702 1496 498
Fragments arranged by size:
1496 910 702 512 498 493 248
BsaWI w'CCGG_w
Cuts at: 2048 2569 3247 3658 3805 4079 2048
Size: 521 678 411 147 274 2828
Fragments arranged by size:
2828 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1111 1141 1111
Size: 30 4829
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 752 1678 1681 1714 752
Size: 926 3 33 3897
Fragments arranged by size:
3897 926 33 3
BseYI C'CCAG_C
Cuts at: 828 1323 2284 3042 3707 4115 828
Size: 495 961 758 665 408 1572
Fragments arranged by size:
1572 961 758 665 495 408
BsiEI CG_ry'CG
Cuts at: 104 1648 2401 2447 3677 104
Size: 1544 753 46 1230 1286
Fragments arranged by size:
1544 1286 1230 753 46
BsiHKAI G_wGCw'C
Cuts at: 824 1433 1873 2651 2841 3701 824
Size: 609 440 778 190 860 1982
Fragments arranged by size:
1982 860 778 609 440 190
BsmI GAATG_Cn'
Cuts at: 852 852
Size: 4859
BsmBI CGTCTCn'nnnn_
Cuts at: 1663 1663
Size: 4859
Bsp1286I G_dGCh'C
Cuts at: 596 824 848 1055 1433 1873 2484 2577 2651
Size: 228 24 207 378 440 611 93 74
Cuts at: 2651 2841 2903 3701 596
Size: 190 62 798 1754
Fragments arranged by size:
1754 798 611 440 378 228 207 190
93 74 62 24
BspHI T'CATG_A
Cuts at: 638 3291 638
Size: 2653 2206
BspMI ACCTGCnnnn'nnnn_
Cuts at: 248 1909 2123 2425 2806 248
Size: 1661 214 302 381 2301
Fragments arranged by size:
2301 1661 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3151 3205 4689 4854 50
Size: 3101 54 1484 165 55
Fragments arranged by size:
3101 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2771 2771
Size: 4859
BsrFI r'CCGG_y
Cuts at: 1643 2857 3038 1643
Size: 1214 181 3464
Fragments arranged by size:
3464 1214 181
BssSI C'ACGA_G
Cuts at: 823 3130 3838 823
Size: 2307 708 1844
Fragments arranged by size:
2307 1844 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2236 2236
Size: 4859
BstBI TT'CG_AA
Cuts at: 429 3220 429
Size: 2791 2068
BstYI r'GATC_y
Cuts at: 1 786 1481 1884 2351 2374 2709 2955 3261
Size: 785 695 403 467 23 335 246 306
Cuts at: 3261 3273 3359 4411 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 785 695 467 449 403 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1612 1612
Size: 4859
BtgI C'CryG_G
Cuts at: 663 1082 1398 1730 1800 663
Size: 419 316 332 70 3722
Fragments arranged by size:
3722 419 332 316 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 285 1532 2809 2990 3153 285
Size: 1247 1277 181 163 1991
Fragments arranged by size:
1991 1277 1247 181 163
BtsI GCAGTG_nn'
Cuts at: 574 1214 1445 2102 574
Size: 640 231 657 3331
Fragments arranged by size:
3331 657 640 231
DraI TTT'AAA
Cuts at: 1858 1858
Size: 4859
DrdI GACnn_nn'nnGTC
Cuts at: 2565 3909 2565
Size: 1344 3515
EaeI y'GGCC_r
Cuts at: 296 869 1085 1179 1645 2444 2618 3009 3036
Size: 573 216 94 466 799 174 391 27
Cuts at: 3036 4381 296
Size: 1345 774
Fragments arranged by size:
1345 799 774 573 466 391 216 174
94 27
EagI C'GGCC_G
Cuts at: 1645 2444 1645
Size: 799 4060
EarI CTCTTCn'nnn_
Cuts at: 1369 1421 2882 3092 1369
Size: 52 1461 210 3136
Fragments arranged by size:
3136 1461 210 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3067 3809 3955 4669 3067
Size: 742 146 714 3257
Fragments arranged by size:
3257 742 714 146
EcoICRI GAG'CTC
Cuts at: 1431 1871 1431
Size: 440 4419
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 1600 1776 2684 3116 3469 4131 189
Size: 1411 176 908 432 353 662 917
Fragments arranged by size:
1411 917 908 662 432 353 176
EcoNI CCTnn'n_nnAGG
Cuts at: 1224 1224
Size: 4859
EcoO109I rG'GnC_Cy
Cuts at: 252 724 1194 1805 1998 252
Size: 472 470 611 193 3113
Fragments arranged by size:
3113 611 472 470 193
EcoRI G'AATT_C
Cuts at: 1863 1863
Size: 4859
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1407 2878 1407
Size: 1471 3388
FseI GG_CCGG'CC
Cuts at: 1647 1647
Size: 4859
FspI TGC'GCA
Cuts at: 2640 2640
Size: 4859
HaeII r_GCGC'y
Cuts at: 276 378 441 1125 2293 2541 3771 4542 276
Size: 102 63 684 1168 248 1230 771 593
Fragments arranged by size:
1230 1168 771 684 593 248 102 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 975 1007 1270 1302 2701 2731 2733 2763 975
Size: 32 263 32 1399 30 2 30 3071
Fragments arranged by size:
3071 1399 263 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1898 1898
Size: 4859
KasI G'GCGC_C
Cuts at: 374 2289 2537 374
Size: 1915 248 2696
Fragments arranged by size:
2696 1915 248
KpnI G_GTAC'C
Cuts at: 1879 1879
Size: 4859
MluI A'CGCG_T
Cuts at: 4292 4292
Size: 4859
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3618 3802 3618
Size: 184 4675
MscI TGG'CCA
Cuts at: 1087 1181 2620 1087
Size: 94 1439 3326
Fragments arranged by size:
3326 1439 94
MslI CAynn'nnrTG
Cuts at: 857 982 1690 2848 2975 857
Size: 125 708 1158 127 2741
Fragments arranged by size:
2741 1158 708 127 125
MspA1I CmG'CkG
Cuts at: 295 1970 2284 2644 3428 3673 295
Size: 1675 314 360 784 245 1481
Fragments arranged by size:
1675 1481 784 360 314 245
NaeI GCC'GGC
Cuts at: 1645 3040 1645
Size: 1395 3464
NarI GG'CG_CC
Cuts at: 375 2290 2538 375
Size: 1915 248 2696
Fragments arranged by size:
2696 1915 248
NcoI C'CATG_G
Cuts at: 663 1800 663
Size: 1137 3722
NgoMIV G'CCGG_C
Cuts at: 1643 3038 1643
Size: 1395 3464
NspI r_CATG'y
Cuts at: 1912 2412 4015 1912
Size: 500 1603 2756
Fragments arranged by size:
2756 1603 500
PasI CC'CwG_GG
Cuts at: 840 840
Size: 4859
PciI A'CATG_T
Cuts at: 4011 4011
Size: 4859
PflMI CCAn_nnn'nTGG
Cuts at: 840 897 1155 840
Size: 57 258 4544
Fragments arranged by size:
4544 258 57
PmeI GTTT'AAAC
Cuts at: 1858 1858
Size: 4859
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 955 987 1111 1143 3305 3337 955
Size: 32 124 32 2162 32 2477
Fragments arranged by size:
2477 2162 124 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 252 1805 252
Size: 1553 3306
PspOMI G'GGCC_C
Cuts at: 1051 1051
Size: 4859
PspXI vC'TCGA_Gb
Cuts at: 847 847
Size: 4859
PstI C_TGCA'G
Cuts at: 1906 2591 1906
Size: 685 4174
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4859
PvuII CAG'CTG
Cuts at: 2284 2644 2284
Size: 360 4499
RsrII CG'GwC_CG
Cuts at: 3054 3054
Size: 4859
SacI G_AGCT'C
Cuts at: 1433 1873 1433
Size: 440 4419
SalI G'TCGA_C
Cuts at: 1896 1896
Size: 4859
SanDI GG'GwC_CC
Cuts at: 1805 1805
Size: 4859
SapI GCTCTTCn'nnn_
Cuts at: 1421 2882 3092 1421
Size: 1461 210 3188
Fragments arranged by size:
3188 1461 210
SbfI CC_TGCA'GG
Cuts at: 1906 1906
Size: 4859
ScaI AGT'ACT
Cuts at: 1411 4376 1411
Size: 2965 1894
SfcI C'TryA_G
Cuts at: 33 223 1902 2587 3555 3746 33
Size: 190 1679 685 968 191 1146
Fragments arranged by size:
1679 1146 968 685 191 190
SfoI GGC'GCC
Cuts at: 376 2291 2539 376
Size: 1915 248 2696
Fragments arranged by size:
2696 1915 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4859
SmaI CCC'GGG
Cuts at: 249 1881 249
Size: 1632 3227
SmlI C'TyrA_G
Cuts at: 847 2014 2929 3643 3905 847
Size: 1167 915 714 262 1801
Fragments arranged by size:
1801 1167 915 714 262
SnaBI TAC'GTA
Cuts at: 259 259
Size: 4859
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4859
SphI G_CATG'C
Cuts at: 1912 2412 1912
Size: 500 4359
StyI C'CwwG_G
Cuts at: 506 663 766 1800 1993 2347 506
Size: 157 103 1034 193 354 3018
Fragments arranged by size:
3018 1034 354 193 157 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 760 1260 2442 2575 3034 3243 760
Size: 500 1182 133 459 209 2376
Fragments arranged by size:
2376 1182 500 459 209 133
TatI w'GTAC_w
Cuts at: 1409 4374 1409
Size: 2965 1894
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 566 598 4376 4408 566
Size: 32 3778 32 1017
Fragments arranged by size:
3778 1017 32 32
Tth111I GACn'n_nGTC
Cuts at: 1849 2656 1849
Size: 807 4052
XbaI T'CTAG_A
Cuts at: 1890 1890
Size: 4859
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 952 1156 2354 952
Size: 204 1198 3457
Fragments arranged by size:
3457 1198 204
XhoI C'TCGA_G
Cuts at: 847 847
Size: 4859
XmaI C'CCGG_G
Cuts at: 247 1879 247
Size: 1632 3227
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AlfI AscI
AvrII BbvCI BfrBI BmgBI BmtI BpmI BsgI BsiWI
BspEI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
DraIII EcoRV FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PfoI PmlI PshAI PsiI
PsrI SacII SexAI SfiI SgrAI SrfI SspI StuI
SwaI XmnI ZraI