Restriction map for
pF1KB5101
(Circular) (Six-base) MAPSORT of: pF1KB5101.seq Check: 7750 from: 1 to: 6260
pF1KB5101 6260 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 640 640
Size: 6260
AatII G_ACGT'C
Cuts at: 1112 1112
Size: 6260
AccI GT'mk_AC
Cuts at: 395 1202 1208 1565 3150 3298 395
Size: 807 6 357 1585 148 3357
Fragments arranged by size:
3357 1585 807 357 148 6
Acc65I G'GTAC_C
Cuts at: 3276 3276
Size: 6260
AclI AA'CG_TT
Cuts at: 6100 6100
Size: 6260
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 717 1729 3019 4085 4517 4870 5532 717
Size: 1012 1290 1066 432 353 662 1445
Fragments arranged by size:
1445 1290 1066 1012 662 432 353
AflIII A'CryG_T
Cuts at: 1710 3612 5412 5693 1710
Size: 1902 1800 281 2277
Fragments arranged by size:
2277 1902 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 2581 2581
Size: 6260
AleI CACnn'nnGTG
Cuts at: 3061 3061
Size: 6260
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 404 404
Size: 6260
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2482 2514 2482
Size: 32 6228
AlwNI CAG_nnn'CTG
Cuts at: 807 1434 1443 1827 2209 2664 2832 3565 5003
Size: 627 9 384 382 455 168 733 1438
Cuts at: 5003 807
Size: 2064
Fragments arranged by size:
2064 1438 733 627 455 384 382 168
9
ApaI G_GGCC'C
Cuts at: 163 272 1402 2882 163
Size: 109 1130 1480 3541
Fragments arranged by size:
3541 1480 1130 109
ApaLI G'TGCA_C
Cuts at: 429 5098 429
Size: 4669 1591
ApoI r'AATT_y
Cuts at: 442 662 946 1894 3264 5613 5869 442
Size: 220 284 948 1370 2349 256 833
Fragments arranged by size:
2349 1370 948 833 284 256 220
AseI AT'TA_AT
Cuts at: 20 20
Size: 6260
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6260
AvaI C'yCGr_G
Cuts at: 264 273 847 1847 2543 2758 2817 2875 3280
Size: 9 574 1000 696 215 59 58 405
Cuts at: 3280 264
Size: 3244
Fragments arranged by size:
3244 1000 696 574 405 215 59 58
9
AvrII C'CTAG_G
Cuts at: 921 3144 921
Size: 2223 4037
BamHI G'GATC_C
Cuts at: 2754 3285 2754
Size: 531 5729
BanI G'GyrC_C
Cuts at: 167 412 628 1146 2167 3205 3276 3690 3938
Size: 245 216 518 1021 1038 71 414 248
Cuts at: 3938 3973 167
Size: 35 2454
Fragments arranged by size:
2454 1038 1021 518 414 248 245 216
71 35
BanII G_rGCy'C
Cuts at: 163 272 280 552 1238 1350 1402 1417 1848
Size: 109 8 272 686 112 52 15 431
Cuts at: 1848 2603 2876 2882 3274 4304 163
Size: 755 273 6 392 1030 2119
Fragments arranged by size:
2119 1030 755 686 431 392 273 272
112 109 52 15 8 6
BbeI G_GCGC'C
Cuts at: 171 2171 3694 3942 171
Size: 2000 1523 248 2489
Fragments arranged by size:
2489 2000 1523 248
BbsI GAAGACnn'nnnn_
Cuts at: 974 2054 974
Size: 1080 5180
BbvCI CC'TCA_GC
Cuts at: 2410 2410
Size: 6260
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 245 279 1838 1872 245
Size: 34 1559 34 4633
Fragments arranged by size:
4633 1559 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 919 4158 5214 6260 919
Size: 3239 1056 1046 919
Fragments arranged by size:
3239 1056 1046 919
BclI T'GATC_A
Cuts at: 857 3780 857
Size: 2923 3337
BglI GCCn_nnn'nGGC
Cuts at: 728 1283 1345 2176 728
Size: 555 62 831 4812
Fragments arranged by size:
4812 831 555 62
BglII A'GATC_T
Cuts at: 1871 2027 3775 5812 1871
Size: 156 1748 2037 2319
Fragments arranged by size:
2319 2037 1748 156
BlpI GC'TnA_GC
Cuts at: 2180 3224 3372 2180
Size: 1044 148 5068
Fragments arranged by size:
5068 1044 148
Bme1580I G_kGCm'C
Cuts at: 163 272 433 631 989 1149 1402 1788 2882
Size: 109 161 198 358 160 253 386 1094
Cuts at: 2882 3885 3978 5102 163
Size: 1003 93 1124 1321
Fragments arranged by size:
1321 1124 1094 1003 386 358 253 198
161 160 109 93
BmgBI CAC'GTC
Cuts at: 2442 2442
Size: 6260
BmrI ACTGGGnnnn_n'
Cuts at: 1951 2518 3472 3553 3888 6032 1951
Size: 567 954 81 335 2144 2179
Fragments arranged by size:
2179 2144 954 567 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1924 4627 1924
Size: 2703 3557
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 688 1785 2186 2232 2767 3221 490
Size: 198 1097 401 46 535 454 3529
Fragments arranged by size:
3529 1097 535 454 401 198 46
Bpu10I CC'TnA_GC
Cuts at: 1681 2254 2410 2471 1681
Size: 573 156 61 5470
Fragments arranged by size:
5470 573 156 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3214 3436 4315 5029 5327 3214
Size: 222 879 714 298 4147
Fragments arranged by size:
4147 879 714 298 222
BsaI GGTCTCn'nnnn_
Cuts at: 1357 1493 2540 5964 1357
Size: 136 1047 3424 1653
Fragments arranged by size:
3424 1653 1047 136
BsaAI yAC'GTr
Cuts at: 3613 3613
Size: 6260
BsaBI GATnn'nnATC
Cuts at: 6 1870 1921 3112 3774 5811 6
Size: 1864 51 1191 662 2037 455
Fragments arranged by size:
2037 1864 1191 662 455 51
BsaHI Gr'CG_yC
Cuts at: 121 168 1109 2168 3095 3691 3939 4641 6137
Size: 47 941 1059 927 596 248 702 1496
Cuts at: 6137 121
Size: 244
Fragments arranged by size:
1496 1059 941 927 702 596 248 244
47
BsaWI w'CCGG_w
Cuts at: 3449 3970 4648 5059 5206 5480 3449
Size: 521 678 411 147 274 4229
Fragments arranged by size:
4229 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 502 532 2184 2214 2482 2512 2836 2866 3179
Size: 30 1652 30 268 30 324 30 313
Cuts at: 3179 3209 502
Size: 30 3553
Fragments arranged by size:
3553 1652 324 313 268 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 514 549 1505 1648 2578 514
Size: 35 956 143 930 4196
Fragments arranged by size:
4196 956 930 143 35
BseYI C'CCAG_C
Cuts at: 155 252 698 1221 1367 1410 2617 3685 4443
Size: 97 446 523 146 43 1207 1068 758
Cuts at: 4443 5108 5516 155
Size: 665 408 899
Fragments arranged by size:
1207 1068 899 758 665 523 446 408
146 97 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 2697 2755 2869 166
Size: 2531 58 114 3557
Fragments arranged by size:
3557 2531 114 58
BsiEI CG_ry'CG
Cuts at: 104 313 3802 3848 5078 104
Size: 209 3489 46 1230 1286
Fragments arranged by size:
3489 1286 1230 209 46
BsiHKAI G_wGCw'C
Cuts at: 348 433 2603 2876 2957 3274 4052 4242 5102
Size: 85 2170 273 81 317 778 190 860
Cuts at: 5102 348
Size: 1506
Fragments arranged by size:
2170 1506 860 778 317 273 190 85
81
BsmI GAATG_Cn'
Cuts at: 2127 2127
Size: 6260
Bsp1286I G_dGCh'C
Cuts at: 163 272 280 348 433 552 631 989 1149
Size: 109 8 68 85 119 79 358 160
Cuts at: 1149 1238 1350 1402 1417 1788 1848 2603 2876
Size: 89 112 52 15 371 60 755 273
Cuts at: 2876 2882 2957 3274 3885 3978 4052 4242 4304
Size: 6 75 317 611 93 74 190 62
Cuts at: 4304 5102 163
Size: 798 1321
Fragments arranged by size:
1321 798 755 611 371 358 317 273
190 160 119 112 109 93 89 85
79 75 74 68 62 60 52 15
8 6
BspHI T'CATG_A
Cuts at: 4692 4692
Size: 6260
BspMI ACCTGCnnnn'nnnn_
Cuts at: 640 1220 2078 2586 3310 3524 3826 4207 640
Size: 580 858 508 724 214 302 381 2693
Fragments arranged by size:
2693 858 724 580 508 381 302 214
BsrBI CCG'CTC
Cuts at: 50 686 1638 4552 4606 6090 6255 50
Size: 636 952 2914 54 1484 165 55
Fragments arranged by size:
2914 1484 952 636 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1518 3073 4172 1518
Size: 1555 1099 3606
Fragments arranged by size:
3606 1555 1099
BsrFI r'CCGG_y
Cuts at: 308 1225 4258 4439 308
Size: 917 3033 181 2129
Fragments arranged by size:
3033 2129 917 181
BsrGI T'GTAC_A
Cuts at: 584 2987 584
Size: 2403 3857
BssSI C'ACGA_G
Cuts at: 4531 5239 4531
Size: 708 5552
BstAPI GCAn_nnn'nTGC
Cuts at: 3637 3637
Size: 6260
BstBI TT'CG_AA
Cuts at: 4621 4621
Size: 6260
BstEII G'GTnAC_C
Cuts at: 2190 2481 2190
Size: 291 5969
BstYI r'GATC_y
Cuts at: 1 803 1871 2027 2405 2754 2828 3285 3752
Size: 802 1068 156 378 349 74 457 467
Cuts at: 3752 3775 4110 4356 4662 4674 4760 5812 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1068 1052 802 467 457 449 378 349
335 306 246 156 86 74 23 12
BstZ17I GTA'TAC
Cuts at: 1566 1566
Size: 6260
Bsu36I CC'TnA_GG
Cuts at: 1184 1879 1975 2507 1184
Size: 695 96 532 4937
Fragments arranged by size:
4937 695 532 96
BtgI C'CryG_G
Cuts at: 107 552 623 1968 2462 3212 107
Size: 445 71 1345 494 750 3155
Fragments arranged by size:
3155 1345 750 494 445 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1908 4210 4391 4554 1908
Size: 2302 181 163 3614
Fragments arranged by size:
3614 2302 181 163
BtsI GCAGTG_nn'
Cuts at: 532 1100 2519 2980 3503 532
Size: 568 1419 461 523 3289
Fragments arranged by size:
3289 1419 568 523 461
DraI TTT'AAA
Cuts at: 3259 3259
Size: 6260
DraIII CAC_nnn'GTG
Cuts at: 144 428 144
Size: 284 5976
DrdI GACnn_nn'nnGTC
Cuts at: 126 3966 5310 126
Size: 3840 1344 1076
Fragments arranged by size:
3840 1344 1076
EaeI y'GGCC_r
Cuts at: 310 756 1097 1260 1275 1337 3053 3845 4019
Size: 446 341 163 15 62 1716 792 174
Cuts at: 4019 4410 4437 5782 310
Size: 391 27 1345 788
Fragments arranged by size:
1716 1345 792 788 446 391 341 174
163 62 27 15
EagI C'GGCC_G
Cuts at: 310 3845 310
Size: 3535 2725
EarI CTCTTCn'nnn_
Cuts at: 4283 4493 4283
Size: 210 6050
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4468 5210 5356 6070 4468
Size: 742 146 714 4658
Fragments arranged by size:
4658 742 714 146
EcoICRI GAG'CTC
Cuts at: 2601 2874 3272 2601
Size: 273 398 5589
Fragments arranged by size:
5589 398 273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 688 717 1729 1785 2186 2232 2767 3019
Size: 198 29 1012 56 401 46 535 252
Cuts at: 3019 3221 4085 4517 4870 5532 490
Size: 202 864 432 353 662 1218
Fragments arranged by size:
1218 1012 864 662 535 432 401 353
252 202 198 56 46 29
EcoNI CCTnn'n_nnAGG
Cuts at: 868 1315 868
Size: 447 5813
EcoO109I rG'GnC_Cy
Cuts at: 159 160 249 268 269 1308 1398 1538 2568
Size: 1 89 19 1 1039 90 140 1030
Cuts at: 2568 2575 2614 2742 2821 2879 3399 159
Size: 7 39 128 79 58 520 3020
Fragments arranged by size:
3020 1039 1030 520 140 128 90 89
79 58 39 19 7 1 1
EcoRI G'AATT_C
Cuts at: 662 946 3264 662
Size: 284 2318 3658
Fragments arranged by size:
3658 2318 284
EcoRV GAT'ATC
Cuts at: 1519 1519
Size: 6260
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2958 4279 2958
Size: 1321 4939
FspI TGC'GCA
Cuts at: 4041 4041
Size: 6260
HaeII r_GCGC'y
Cuts at: 171 2171 3694 3942 5172 5943 171
Size: 2000 1523 248 1230 771 488
Fragments arranged by size:
2000 1523 1230 771 488 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 502 534 1027 1059 1894 1926 2567 2599 2813
Size: 32 493 32 835 32 641 32 214
Cuts at: 2813 2845 2889 2921 4102 4132 4134 4164 502
Size: 32 44 32 1181 30 2 30 2598
Fragments arranged by size:
2598 1181 835 641 493 214 44 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3299 3299
Size: 6260
HindIII A'AGCT_T
Cuts at: 3172 3172
Size: 6260
KasI G'GCGC_C
Cuts at: 167 2167 3690 3938 167
Size: 2000 1523 248 2489
Fragments arranged by size:
2489 2000 1523 248
KpnI G_GTAC'C
Cuts at: 3280 3280
Size: 6260
MluI A'CGCG_T
Cuts at: 5693 5693
Size: 6260
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2732 5019 5203 2732
Size: 2287 184 3789
Fragments arranged by size:
3789 2287 184
MscI TGG'CCA
Cuts at: 1262 1339 3055 4021 1262
Size: 77 1716 966 3501
Fragments arranged by size:
3501 1716 966 77
MslI CAynn'nnrTG
Cuts at: 2059 2594 3061 3211 4249 4376 2059
Size: 535 467 150 1038 127 3943
Fragments arranged by size:
3943 1038 535 467 150 127
MspA1I CmG'CkG
Cuts at: 150 180 189 590 698 879 1749 2076 2651
Size: 30 9 401 108 181 870 327 575
Cuts at: 2651 2949 3030 3061 3371 3685 4045 4829 5074
Size: 298 81 31 310 314 360 784 245
Cuts at: 5074 150
Size: 1336
Fragments arranged by size:
1336 870 784 575 401 360 327 314
310 298 245 181 108 81 31 30
9
NaeI GCC'GGC
Cuts at: 1227 4441 1227
Size: 3214 3046
NarI GG'CG_CC
Cuts at: 168 2168 3691 3939 168
Size: 2000 1523 248 2489
Fragments arranged by size:
2489 2000 1523 248
NcoI C'CATG_G
Cuts at: 107 552 3212 107
Size: 445 2660 3155
Fragments arranged by size:
3155 2660 445
NdeI CA'TA_TG
Cuts at: 3201 3201
Size: 6260
NgoMIV G'CCGG_C
Cuts at: 1225 4439 1225
Size: 3214 3046
NspI r_CATG'y
Cuts at: 575 3313 3813 5416 575
Size: 2738 500 1603 1419
Fragments arranged by size:
2738 1603 1419 500
PasI CC'CwG_GG
Cuts at: 218 1302 2236 218
Size: 1084 934 4242
Fragments arranged by size:
4242 1084 934
PciI A'CATG_T
Cuts at: 5412 5412
Size: 6260
PflMI CCAn_nnn'nTGG
Cuts at: 2154 2336 2664 2154
Size: 182 328 5750
Fragments arranged by size:
5750 328 182
PfoI T'CCnGG_A
Cuts at: 467 2369 467
Size: 1902 4358
PmeI GTTT'AAAC
Cuts at: 3259 3259
Size: 6260
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2482 2514 4706 4738 2482
Size: 32 2192 32 4004
Fragments arranged by size:
4004 2192 32 32
PpuMI rG'GwC_Cy
Cuts at: 1308 1538 2568 2575 2821 1308
Size: 230 1030 7 246 4747
Fragments arranged by size:
4747 1030 246 230 7
PspOMI G'GGCC_C
Cuts at: 159 268 1398 2878 159
Size: 109 1130 1480 3541
Fragments arranged by size:
3541 1480 1130 109
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 988 1020 2089 2121 988
Size: 32 1069 32 5127
Fragments arranged by size:
5127 1069 32 32
PstI C_TGCA'G
Cuts at: 383 884 1217 1439 1648 2075 2912 2975 3307
Size: 501 333 222 209 427 837 63 332
Cuts at: 3307 3992 383
Size: 685 2651
Fragments arranged by size:
2651 837 685 501 427 333 332 222
209 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6260
PvuII CAG'CTG
Cuts at: 150 590 698 879 1749 2076 2651 3030 3061
Size: 440 108 181 870 327 575 379 31
Cuts at: 3061 3685 4045 150
Size: 624 360 2365
Fragments arranged by size:
2365 870 624 575 440 379 360 327
181 108 31
RsrII CG'GwC_CG
Cuts at: 4455 4455
Size: 6260
SacI G_AGCT'C
Cuts at: 2603 2876 3274 2603
Size: 273 398 5589
Fragments arranged by size:
5589 398 273
SalI G'TCGA_C
Cuts at: 3297 3297
Size: 6260
SapI GCTCTTCn'nnn_
Cuts at: 4283 4493 4283
Size: 210 6050
SbfI CC_TGCA'GG
Cuts at: 3307 3307
Size: 6260
ScaI AGT'ACT
Cuts at: 260 2215 5777 260
Size: 1955 3562 743
Fragments arranged by size:
3562 1955 743
SexAI A'CCwGG_T
Cuts at: 863 863
Size: 6260
SfcI C'TryA_G
Cuts at: 33 379 880 1213 1435 1644 2071 2908 2971
Size: 346 501 333 222 209 427 837 63
Cuts at: 2971 3303 3988 4956 5147 33
Size: 332 685 968 191 1146
Fragments arranged by size:
1146 968 837 685 501 427 346 333
332 222 209 191 63
SfoI GGC'GCC
Cuts at: 169 2169 3692 3940 169
Size: 2000 1523 248 2489
Fragments arranged by size:
2489 2000 1523 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6260
SmaI CCC'GGG
Cuts at: 275 3282 275
Size: 3007 3253
SmlI C'TyrA_G
Cuts at: 1847 3229 3415 4330 5044 5306 1847
Size: 1382 186 915 714 262 2801
Fragments arranged by size:
2801 1382 915 714 262 186
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6260
SphI G_CATG'C
Cuts at: 575 3313 3813 575
Size: 2738 500 3022
Fragments arranged by size:
3022 2738 500
StyI C'CwwG_G
Cuts at: 107 350 406 552 723 921 1940 2610 3074
Size: 243 56 146 171 198 1019 670 464
Cuts at: 3074 3144 3212 3394 3748 107
Size: 70 68 182 354 2619
Fragments arranged by size:
2619 1019 670 464 354 243 198 182
171 146 70 68 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 231 363 1235 2101 2146 2249 3843 3976 4435
Size: 132 872 866 45 103 1594 133 459
Cuts at: 4435 4644 231
Size: 209 1847
Fragments arranged by size:
1847 1594 872 866 459 209 133 132
103 45
TatI w'GTAC_w
Cuts at: 258 512 584 2213 2987 5775 258
Size: 254 72 1629 774 2788 743
Fragments arranged by size:
2788 1629 774 743 254 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 281 313 917 949 2184 2216 2836 2868 5777
Size: 32 604 32 1235 32 620 32 2909
Cuts at: 5777 5809 281
Size: 32 732
Fragments arranged by size:
2909 1235 732 620 604 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 325 4057 325
Size: 3732 2528
XbaI T'CTAG_A
Cuts at: 3291 3291
Size: 6260
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 587 1270 2531 3755 587
Size: 683 1261 1224 3092
Fragments arranged by size:
3092 1261 1224 683
XhoI C'TCGA_G
Cuts at: 1847 1847
Size: 6260
XmaI C'CCGG_G
Cuts at: 273 3280 273
Size: 3007 3253
ZraI GAC'GTC
Cuts at: 1110 1110
Size: 6260
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AscI BaeI BfrBI BmtI BsiWI
BsmBI BspEI BssHII BstXI ClaI CspCI FseI FspAI
HpaI MfeI NheI NotI NruI NsiI PacI PmlI
PshAI PsiI PspXI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XmnI