Restriction map for
pF1KB5104
(Circular) (Six-base) MAPSORT of: pF1KB5104.seq Check: 6813 from: 1 to: 4757
pF1KB5104 4757 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1795 1795
Size: 4757
Acc65I G'GTAC_C
Cuts at: 1773 1773
Size: 4757
AclI AA'CG_TT
Cuts at: 4597 4597
Size: 4757
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 143 233 286 379 2582 3014 3367 4029 143
Size: 90 53 93 2203 432 353 662 871
Fragments arranged by size:
2203 871 662 432 353 93 90 53
AflIII A'CryG_T
Cuts at: 772 2109 3909 4190 772
Size: 1337 1800 281 1339
Fragments arranged by size:
1800 1339 1337 281
AleI CACnn'nnGTG
Cuts at: 361 361
Size: 4757
AlwNI CAG_nnn'CTG
Cuts at: 402 405 2062 3500 402
Size: 3 1657 1438 1659
Fragments arranged by size:
1659 1657 1438 3
ApaLI G'TGCA_C
Cuts at: 3595 3595
Size: 4757
ApoI r'AATT_y
Cuts at: 293 412 529 763 1119 1665 1761 4110 4366
Size: 119 117 234 356 546 96 2349 256
Cuts at: 4366 293
Size: 684
Fragments arranged by size:
2349 684 546 356 256 234 119 117
96
AseI AT'TA_AT
Cuts at: 20 1310 20
Size: 1290 3467
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4757
AvaI C'yCGr_G
Cuts at: 1777 1777
Size: 4757
BamHI G'GATC_C
Cuts at: 1782 1782
Size: 4757
BanI G'GyrC_C
Cuts at: 207 1464 1773 2187 2435 2470 207
Size: 1257 309 414 248 35 2494
Fragments arranged by size:
2494 1257 414 309 248 35
BanII G_rGCy'C
Cuts at: 177 1103 1771 2801 177
Size: 926 668 1030 2133
Fragments arranged by size:
2133 1030 926 668
BbeI G_GCGC'C
Cuts at: 2191 2439 2191
Size: 248 4509
BbsI GAAGACnn'nnnn_
Cuts at: 1196 1196
Size: 4757
BbvCI CC'TCA_GC
Cuts at: 524 524
Size: 4757
BciVI GTATCCnnnnn_n'
Cuts at: 2655 3711 4757 2655
Size: 1056 1046 2655
Fragments arranged by size:
2655 1056 1046
BclI T'GATC_A
Cuts at: 515 2277 515
Size: 1762 2995
BglII A'GATC_T
Cuts at: 2272 4309 2272
Size: 2037 2720
BlpI GC'TnA_GC
Cuts at: 1869 1869
Size: 4757
Bme1580I G_kGCm'C
Cuts at: 210 1425 2382 2475 3599 210
Size: 1215 957 93 1124 1368
Fragments arranged by size:
1368 1215 1124 957 93
BmrI ACTGGGnnnn_n'
Cuts at: 1969 2050 2385 4529 1969
Size: 81 335 2144 2197
Fragments arranged by size:
2197 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3124 3124
Size: 4757
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1436 1678 1436
Size: 242 4515
Bpu10I CC'TnA_GC
Cuts at: 524 524
Size: 4757
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1219 1359 1933 2812 3526 3824 1219
Size: 140 574 879 714 298 2152
Fragments arranged by size:
2152 879 714 574 298 140
BsaI GGTCTCn'nnnn_
Cuts at: 4461 4461
Size: 4757
BsaAI yAC'GTr
Cuts at: 2110 2110
Size: 4757
BsaBI GATnn'nnATC
Cuts at: 6 2271 4308 6
Size: 2265 2037 455
Fragments arranged by size:
2265 2037 455
BsaHI Gr'CG_yC
Cuts at: 1734 2188 2436 3138 4634 1734
Size: 454 248 702 1496 1857
Fragments arranged by size:
1857 1496 702 454 248
BsaWI w'CCGG_w
Cuts at: 1123 1946 2467 3145 3556 3703 3977 1123
Size: 823 521 678 411 147 274 1903
Fragments arranged by size:
1903 823 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1102 2182 2940 3605 4013 1102
Size: 1080 758 665 408 1846
Fragments arranged by size:
1846 1080 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2299 2345 3575 104
Size: 2195 46 1230 1286
Fragments arranged by size:
2195 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 177 1771 2549 2739 3599 177
Size: 1594 778 190 860 1335
Fragments arranged by size:
1594 1335 860 778 190
BsmI GAATG_Cn'
Cuts at: 461 561 461
Size: 100 4657
Bsp1286I G_dGCh'C
Cuts at: 177 210 1103 1425 1771 2382 2475 2549 2739
Size: 33 893 322 346 611 93 74 190
Cuts at: 2739 2801 3599 177
Size: 62 798 1335
Fragments arranged by size:
1335 893 798 611 346 322 190 93
74 62 33
BspEI T'CCGG_A
Cuts at: 1123 1123
Size: 4757
BspHI T'CATG_A
Cuts at: 3189 3189
Size: 4757
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1807 2021 2323 2704 1807
Size: 214 302 381 3860
Fragments arranged by size:
3860 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3049 3103 4587 4752 50
Size: 2999 54 1484 165 55
Fragments arranged by size:
2999 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2669 2669
Size: 4757
BsrFI r'CCGG_y
Cuts at: 2755 2936 2755
Size: 181 4576
BssSI C'ACGA_G
Cuts at: 3028 3736 3028
Size: 708 4049
BstAPI GCAn_nnn'nTGC
Cuts at: 405 844 2134 405
Size: 439 1290 3028
Fragments arranged by size:
3028 1290 439
BstBI TT'CG_AA
Cuts at: 3118 3118
Size: 4757
BstXI CCAn_nnnn'nTGG
Cuts at: 643 1050 1110 643
Size: 407 60 4290
Fragments arranged by size:
4290 407 60
BstYI r'GATC_y
Cuts at: 1 468 828 1782 2249 2272 2607 2853 3159
Size: 467 360 954 467 23 335 246 306
Cuts at: 3159 3171 3257 4309 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 954 467 467 449 360 335 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 1043 107
Size: 936 3821
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2707 2888 3051 2707
Size: 181 163 4413
Fragments arranged by size:
4413 181 163
BtsI GCAGTG_nn'
Cuts at: 368 2000 368
Size: 1632 3125
DraI TTT'AAA
Cuts at: 548 1756 548
Size: 1208 3549
DrdI GACnn_nn'nnGTC
Cuts at: 2463 3807 2463
Size: 1344 3413
EaeI y'GGCC_r
Cuts at: 2342 2516 2907 2934 4279 2342
Size: 174 391 27 1345 2820
Fragments arranged by size:
2820 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2342 2342
Size: 4757
EarI CTCTTCn'nnn_
Cuts at: 437 1679 2780 2990 437
Size: 1242 1101 210 2204
Fragments arranged by size:
2204 1242 1101 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2965 3707 3853 4567 2965
Size: 742 146 714 3155
Fragments arranged by size:
3155 742 714 146
EcoICRI GAG'CTC
Cuts at: 175 1769 175
Size: 1594 3163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 143 233 286 379 1436 1678 2582 3014 3367
Size: 90 53 93 1057 242 904 432 353
Cuts at: 3367 4029 143
Size: 662 871
Fragments arranged by size:
1057 904 871 662 432 353 242 93
90 53
EcoO109I rG'GnC_Cy
Cuts at: 701 1896 701
Size: 1195 3562
EcoRI G'AATT_C
Cuts at: 529 763 1761 529
Size: 234 998 3525
Fragments arranged by size:
3525 998 234
EcoRV GAT'ATC
Cuts at: 681 681
Size: 4757
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 218 409 849 2776 218
Size: 191 440 1927 2199
Fragments arranged by size:
2199 1927 440 191
FspI TGC'GCA
Cuts at: 2538 2538
Size: 4757
HaeII r_GCGC'y
Cuts at: 2191 2439 3669 4440 2191
Size: 248 1230 771 2508
Fragments arranged by size:
2508 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 218 250 1465 1497 2599 2629 2631 2661 218
Size: 32 1215 32 1102 30 2 30 2314
Fragments arranged by size:
2314 1215 1102 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1361 1796 1361
Size: 435 4322
HindIII A'AGCT_T
Cuts at: 1343 1343
Size: 4757
HpaI GTT'AAC
Cuts at: 1361 1361
Size: 4757
KasI G'GCGC_C
Cuts at: 2187 2435 2187
Size: 248 4509
KpnI G_GTAC'C
Cuts at: 1777 1777
Size: 4757
MluI A'CGCG_T
Cuts at: 4190 4190
Size: 4757
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1288 1491 3516 3700 1288
Size: 203 2025 184 2345
Fragments arranged by size:
2345 2025 203 184
MscI TGG'CCA
Cuts at: 2518 2518
Size: 4757
MslI CAynn'nnrTG
Cuts at: 245 361 1037 1499 2746 2873 245
Size: 116 676 462 1247 127 2129
Fragments arranged by size:
2129 1247 676 462 127 116
MspA1I CmG'CkG
Cuts at: 306 402 1057 1868 2182 2542 3326 3571 306
Size: 96 655 811 314 360 784 245 1492
Fragments arranged by size:
1492 811 784 655 360 314 245 96
NaeI GCC'GGC
Cuts at: 2938 2938
Size: 4757
NarI GG'CG_CC
Cuts at: 2188 2436 2188
Size: 248 4509
NcoI C'CATG_G
Cuts at: 107 1043 107
Size: 936 3821
NdeI CA'TA_TG
Cuts at: 242 242
Size: 4757
NgoMIV G'CCGG_C
Cuts at: 2936 2936
Size: 4757
NspI r_CATG'y
Cuts at: 319 1810 2310 3913 319
Size: 1491 500 1603 1163
Fragments arranged by size:
1603 1491 1163 500
PciI A'CATG_T
Cuts at: 3909 3909
Size: 4757
PmeI GTTT'AAAC
Cuts at: 1756 1756
Size: 4757
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3203 3235 3203
Size: 32 4725
PpuMI rG'GwC_Cy
Cuts at: 701 701
Size: 4757
PsiI TTA'TAA
Cuts at: 491 491
Size: 4757
PstI C_TGCA'G
Cuts at: 1804 2489 1804
Size: 685 4072
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4757
PvuII CAG'CTG
Cuts at: 306 402 1057 2182 2542 306
Size: 96 655 1125 360 2521
Fragments arranged by size:
2521 1125 655 360 96
RsrII CG'GwC_CG
Cuts at: 2952 2952
Size: 4757
SacI G_AGCT'C
Cuts at: 177 1771 177
Size: 1594 3163
SalI G'TCGA_C
Cuts at: 1794 1794
Size: 4757
SapI GCTCTTCn'nnn_
Cuts at: 2780 2990 2780
Size: 210 4547
SbfI CC_TGCA'GG
Cuts at: 1804 1804
Size: 4757
ScaI AGT'ACT
Cuts at: 4274 4274
Size: 4757
SfcI C'TryA_G
Cuts at: 33 1282 1800 2485 3453 3644 33
Size: 1249 518 685 968 191 1146
Fragments arranged by size:
1249 1146 968 685 518 191
SfoI GGC'GCC
Cuts at: 2189 2437 2189
Size: 248 4509
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4757
SmaI CCC'GGG
Cuts at: 1779 1779
Size: 4757
SmlI C'TyrA_G
Cuts at: 1234 1374 1912 2827 3541 3803 1234
Size: 140 538 915 714 262 2188
Fragments arranged by size:
2188 915 714 538 262 140
SpeI A'CTAG_T
Cuts at: 67 1093 67
Size: 1026 3731
SphI G_CATG'C
Cuts at: 1810 2310 1810
Size: 500 4257
StuI AGG'CCT
Cuts at: 866 866
Size: 4757
StyI C'CwwG_G
Cuts at: 107 1043 1891 2245 107
Size: 936 848 354 2619
Fragments arranged by size:
2619 936 848 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1368 2340 2473 2932 3141 1368
Size: 972 133 459 209 2984
Fragments arranged by size:
2984 972 459 209 133
TatI w'GTAC_w
Cuts at: 4272 4272
Size: 4757
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 764 796 4274 4306 764
Size: 32 3478 32 1215
Fragments arranged by size:
3478 1215 32 32
Tth111I GACn'n_nGTC
Cuts at: 2554 2554
Size: 4757
XbaI T'CTAG_A
Cuts at: 1788 1788
Size: 4757
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1458 2252 1458
Size: 794 3963
XmaI C'CCGG_G
Cuts at: 1777 1777
Size: 4757
XmnI GAAnn'nnTTC
Cuts at: 737 763 1640 737
Size: 26 877 3854
Fragments arranged by size:
3854 877 26
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BsaXI BseRI BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI MfeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI