Restriction map for
pF1KB5105
(Circular) (Six-base) MAPSORT of: pF1KB5105.seq Check: 5402 from: 1 to: 4964
pF1KB5105 4964 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 2002 2002
Size: 4964
Acc65I G'GTAC_C
Cuts at: 1980 1980
Size: 4964
AclI AA'CG_TT
Cuts at: 4804 4804
Size: 4964
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 983 2789 3221 3574 4236 983
Size: 1806 432 353 662 1711
Fragments arranged by size:
1806 1711 662 432 353
AflIII A'CryG_T
Cuts at: 476 2316 4116 4397 476
Size: 1840 1800 281 1043
Fragments arranged by size:
1840 1800 1043 281
AleI CACnn'nnGTG
Cuts at: 721 721
Size: 4964
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1495 1495
Size: 4964
AlwNI CAG_nnn'CTG
Cuts at: 187 1700 2269 3707 187
Size: 1513 569 1438 1444
Fragments arranged by size:
1513 1444 1438 569
ApaLI G'TGCA_C
Cuts at: 3802 3802
Size: 4964
ApoI r'AATT_y
Cuts at: 691 1550 1861 1968 4317 4573 691
Size: 859 311 107 2349 256 1082
Fragments arranged by size:
2349 1082 859 311 256 107
AseI AT'TA_AT
Cuts at: 20 20
Size: 4964
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4964
AvaI C'yCGr_G
Cuts at: 1984 1984
Size: 4964
BamHI G'GATC_C
Cuts at: 1869 1989 1869
Size: 120 4844
BanI G'GyrC_C
Cuts at: 1243 1980 2394 2642 2677 1243
Size: 737 414 248 35 3530
Fragments arranged by size:
3530 737 414 248 35
BanII G_rGCy'C
Cuts at: 989 1978 3008 989
Size: 989 1030 2945
Fragments arranged by size:
2945 1030 989
BbeI G_GCGC'C
Cuts at: 2398 2646 2398
Size: 248 4716
BbsI GAAGACnn'nnnn_
Cuts at: 1574 1931 1574
Size: 357 4607
BbvCI CC'TCA_GC
Cuts at: 1172 1172
Size: 4964
BciVI GTATCCnnnnn_n'
Cuts at: 2862 3918 4964 2862
Size: 1056 1046 2862
Fragments arranged by size:
2862 1056 1046
BclI T'GATC_A
Cuts at: 2484 2484
Size: 4964
BfrBI ATG'CAT
Cuts at: 1939 1939
Size: 4964
BglI GCCn_nnn'nGGC
Cuts at: 1252 1252
Size: 4964
BglII A'GATC_T
Cuts at: 2479 4516 2479
Size: 2037 2927
BlpI GC'TnA_GC
Cuts at: 2076 2076
Size: 4964
Bme1580I G_kGCm'C
Cuts at: 1248 2589 2682 3806 1248
Size: 1341 93 1124 2406
Fragments arranged by size:
2406 1341 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2176 2257 2592 4736 2176
Size: 81 335 2144 2404
Fragments arranged by size:
2404 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1855 3331 1855
Size: 1476 3488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 875 1654 875
Size: 779 4185
Bpu10I CC'TnA_GC
Cuts at: 1172 1544 1172
Size: 372 4592
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2140 3019 3733 4031 2140
Size: 879 714 298 3073
Fragments arranged by size:
3073 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 183 4668 183
Size: 4485 479
BsaAI yAC'GTr
Cuts at: 2317 2317
Size: 4964
BsaBI GATnn'nnATC
Cuts at: 6 2478 4515 6
Size: 2472 2037 455
Fragments arranged by size:
2472 2037 455
BsaHI Gr'CG_yC
Cuts at: 2395 2643 3345 4841 2395
Size: 248 702 1496 2518
Fragments arranged by size:
2518 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2153 2674 3352 3763 3910 4184 2153
Size: 521 678 411 147 274 2933
Fragments arranged by size:
2933 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 326 356 461 491 326
Size: 30 105 30 4799
Fragments arranged by size:
4799 105 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 482 482
Size: 4964
BseYI C'CCAG_C
Cuts at: 2389 3147 3812 4220 2389
Size: 758 665 408 3133
Fragments arranged by size:
3133 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 236 236
Size: 4964
BsiEI CG_ry'CG
Cuts at: 104 2506 2552 3782 104
Size: 2402 46 1230 1286
Fragments arranged by size:
2402 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 989 1826 1978 2756 2946 3806 989
Size: 837 152 778 190 860 2147
Fragments arranged by size:
2147 860 837 778 190 152
BsmI GAATG_Cn'
Cuts at: 1939 1941 1939
Size: 2 4962
Bsp1286I G_dGCh'C
Cuts at: 989 1248 1826 1978 2589 2682 2756 2946 3008
Size: 259 578 152 611 93 74 190 62
Cuts at: 3008 3806 989
Size: 798 2147
Fragments arranged by size:
2147 798 611 578 259 190 152 93
74 62
BspHI T'CATG_A
Cuts at: 3396 3396
Size: 4964
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2014 2228 2530 2911 2014
Size: 214 302 381 4067
Fragments arranged by size:
4067 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3256 3310 4794 4959 50
Size: 3206 54 1484 165 55
Fragments arranged by size:
3206 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 641 2876 641
Size: 2235 2729
BsrFI r'CCGG_y
Cuts at: 2962 3143 2962
Size: 181 4783
BsrGI T'GTAC_A
Cuts at: 1584 1584
Size: 4964
BssSI C'ACGA_G
Cuts at: 3235 3943 3235
Size: 708 4256
BstAPI GCAn_nnn'nTGC
Cuts at: 2341 2341
Size: 4964
BstBI TT'CG_AA
Cuts at: 3325 3325
Size: 4964
BstYI r'GATC_y
Cuts at: 1 336 1869 1989 2456 2479 2814 3060 3366
Size: 335 1533 120 467 23 335 246 306
Cuts at: 3366 3378 3464 4516 1
Size: 12 86 1052 449
Fragments arranged by size:
1533 1052 467 449 335 335 306 246
120 86 23 12
BtgI C'CryG_G
Cuts at: 107 719 107
Size: 612 4352
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2914 3095 3258 2914
Size: 181 163 4620
Fragments arranged by size:
4620 181 163
BtsI GCAGTG_nn'
Cuts at: 1778 2207 1778
Size: 429 4535
DraI TTT'AAA
Cuts at: 1963 1963
Size: 4964
DraIII CAC_nnn'GTG
Cuts at: 1348 1348
Size: 4964
DrdI GACnn_nn'nnGTC
Cuts at: 2670 4014 2670
Size: 1344 3620
EaeI y'GGCC_r
Cuts at: 795 2549 2723 3114 3141 4486 795
Size: 1754 174 391 27 1345 1273
Fragments arranged by size:
1754 1345 1273 391 174 27
EagI C'GGCC_G
Cuts at: 2549 2549
Size: 4964
EarI CTCTTCn'nnn_
Cuts at: 1021 2987 3197 1021
Size: 1966 210 2788
Fragments arranged by size:
2788 1966 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 569 3172 3914 4060 4774 569
Size: 2603 742 146 714 759
Fragments arranged by size:
2603 759 742 714 146
EcoICRI GAG'CTC
Cuts at: 987 1976 987
Size: 989 3975
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 875 983 1654 2789 3221 3574 4236 875
Size: 108 671 1135 432 353 662 1603
Fragments arranged by size:
1603 1135 671 662 432 353 108
EcoO109I rG'GnC_Cy
Cuts at: 184 1176 2103 184
Size: 992 927 3045
Fragments arranged by size:
3045 992 927
EcoRI G'AATT_C
Cuts at: 1968 1968
Size: 4964
EcoRV GAT'ATC
Cuts at: 432 498 884 1228 432
Size: 66 386 344 4168
Fragments arranged by size:
4168 386 344 66
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2983 2983
Size: 4964
FspI TGC'GCA
Cuts at: 2745 2745
Size: 4964
HaeII r_GCGC'y
Cuts at: 2398 2646 3876 4647 2398
Size: 248 1230 771 2715
Fragments arranged by size:
2715 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1144 1176 2806 2836 2838 2868 1144
Size: 32 1630 30 2 30 3240
Fragments arranged by size:
3240 1630 32 30 30 2
HincII GTy'rAC
Cuts at: 791 2003 791
Size: 1212 3752
KasI G'GCGC_C
Cuts at: 2394 2642 2394
Size: 248 4716
KpnI G_GTAC'C
Cuts at: 1984 1984
Size: 4964
MfeI C'AATT_G
Cuts at: 1452 1452
Size: 4964
MluI A'CGCG_T
Cuts at: 4397 4397
Size: 4964
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 240 312 407 1757 3723 3907 240
Size: 72 95 1350 1966 184 1297
Fragments arranged by size:
1966 1350 1297 184 95 72
MscI TGG'CCA
Cuts at: 797 2725 797
Size: 1928 3036
MslI CAynn'nnrTG
Cuts at: 721 2953 3080 721
Size: 2232 127 2605
Fragments arranged by size:
2605 2232 127
MspA1I CmG'CkG
Cuts at: 2075 2389 2749 3533 3778 2075
Size: 314 360 784 245 3261
Fragments arranged by size:
3261 784 360 314 245
NaeI GCC'GGC
Cuts at: 3145 3145
Size: 4964
NarI GG'CG_CC
Cuts at: 2395 2643 2395
Size: 248 4716
NcoI C'CATG_G
Cuts at: 107 719 107
Size: 612 4352
NgoMIV G'CCGG_C
Cuts at: 3143 3143
Size: 4964
NsiI A_TGCA'T
Cuts at: 1941 1941
Size: 4964
NspI r_CATG'y
Cuts at: 1932 2017 2517 4120 1932
Size: 85 500 1603 2776
Fragments arranged by size:
2776 1603 500 85
PciI A'CATG_T
Cuts at: 4116 4116
Size: 4964
PflMI CCAn_nnn'nTGG
Cuts at: 1348 1348
Size: 4964
PfoI T'CCnGG_A
Cuts at: 971 971
Size: 4964
PmeI GTTT'AAAC
Cuts at: 1963 1963
Size: 4964
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3410 3442 3410
Size: 32 4932
PpuMI rG'GwC_Cy
Cuts at: 184 184
Size: 4964
PshAI GACnn'nnGTC
Cuts at: 423 423
Size: 4964
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 731 763 731
Size: 32 4932
PstI C_TGCA'G
Cuts at: 1218 1559 2011 2696 1218
Size: 341 452 685 3486
Fragments arranged by size:
3486 685 452 341
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4964
PvuII CAG'CTG
Cuts at: 2389 2749 2389
Size: 360 4604
RsrII CG'GwC_CG
Cuts at: 3159 3159
Size: 4964
SacI G_AGCT'C
Cuts at: 989 1978 989
Size: 989 3975
SalI G'TCGA_C
Cuts at: 2001 2001
Size: 4964
SapI GCTCTTCn'nnn_
Cuts at: 2987 3197 2987
Size: 210 4754
SbfI CC_TGCA'GG
Cuts at: 2011 2011
Size: 4964
ScaI AGT'ACT
Cuts at: 1114 4481 1114
Size: 3367 1597
SexAI A'CCwGG_T
Cuts at: 1344 1344
Size: 4964
SfcI C'TryA_G
Cuts at: 33 368 1214 1467 1555 2007 2692 3660 3851
Size: 335 846 253 88 452 685 968 191
Cuts at: 3851 33
Size: 1146
Fragments arranged by size:
1146 968 846 685 452 335 253 191
88
SfoI GGC'GCC
Cuts at: 2396 2644 2396
Size: 248 4716
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4964
SmaI CCC'GGG
Cuts at: 1986 1986
Size: 4964
SmlI C'TyrA_G
Cuts at: 2119 3034 3748 4010 2119
Size: 915 714 262 3073
Fragments arranged by size:
3073 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4964
SphI G_CATG'C
Cuts at: 2017 2517 2017
Size: 500 4464
StyI C'CwwG_G
Cuts at: 107 719 1487 2098 2452 107
Size: 612 768 611 354 2619
Fragments arranged by size:
2619 768 612 611 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2547 2680 3139 3348 2547
Size: 133 459 209 4163
Fragments arranged by size:
4163 459 209 133
TatI w'GTAC_w
Cuts at: 342 1112 1584 4479 342
Size: 770 472 2895 827
Fragments arranged by size:
2895 827 770 472
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 461 493 863 895 4481 4513 461
Size: 32 370 32 3586 32 912
Fragments arranged by size:
3586 912 370 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2761 2761
Size: 4964
XbaI T'CTAG_A
Cuts at: 1162 1995 1162
Size: 833 4131
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 228 2459 228
Size: 2231 2733
XmaI C'CCGG_G
Cuts at: 1984 1984
Size: 4964
XmnI GAAnn'nnTTC
Cuts at: 1602 1939 1602
Size: 337 4627
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI ApaI
AscI AvrII BaeI BcgI BmgBI BmtI BsiWI BsmBI
BspEI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EcoNI FseI FspAI HindIII HpaI NdeI NheI NotI
NruI PacI PasI PmlI PsiI PspOMI PspXI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI