Restriction map for
pF1KB5106
(Circular) (Six-base) MAPSORT of: pF1KB5106.seq Check: 3647 from: 1 to: 5465
pF1KB5106 5465 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1801 1801
Size: 5465
AccI GT'mk_AC
Cuts at: 701 2503 701
Size: 1802 3663
Acc65I G'GTAC_C
Cuts at: 773 2481 773
Size: 1708 3757
AclI AA'CG_TT
Cuts at: 2285 5305 2285
Size: 3020 2445
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 944 2332 3290 3722 4075 4737 451
Size: 493 1388 958 432 353 662 1179
Fragments arranged by size:
1388 1179 958 662 493 432 353
AflIII A'CryG_T
Cuts at: 1006 1008 1548 2414 2439 2817 4617 4898 1006
Size: 2 540 866 25 378 1800 281 1573
Fragments arranged by size:
1800 1573 866 540 378 281 25 2
AleI CACnn'nnGTG
Cuts at: 1009 1009
Size: 5465
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 466 466
Size: 5465
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 802 834 802
Size: 32 5433
AlwNI CAG_nnn'CTG
Cuts at: 2770 4208 2770
Size: 1438 4027
ApaI G_GGCC'C
Cuts at: 132 132
Size: 5465
ApaLI G'TGCA_C
Cuts at: 4303 4303
Size: 5465
ApoI r'AATT_y
Cuts at: 2236 2469 4818 5074 2236
Size: 233 2349 256 2627
Fragments arranged by size:
2627 2349 256 233
AseI AT'TA_AT
Cuts at: 20 20
Size: 5465
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5465
AvaI C'yCGr_G
Cuts at: 125 1021 1557 1670 2219 2485 125
Size: 896 536 113 549 266 3105
Fragments arranged by size:
3105 896 549 536 266 113
BamHI G'GATC_C
Cuts at: 2490 2490
Size: 5465
BanI G'GyrC_C
Cuts at: 398 646 773 1365 2136 2481 2895 3143 3178
Size: 248 127 592 771 345 414 248 35
Cuts at: 3178 398
Size: 2685
Fragments arranged by size:
2685 771 592 414 345 248 248 127
35
BanII G_rGCy'C
Cuts at: 132 142 625 1895 1934 2479 3509 132
Size: 10 483 1270 39 545 1030 2088
Fragments arranged by size:
2088 1270 1030 545 483 39 10
BbeI G_GCGC'C
Cuts at: 2899 3147 2899
Size: 248 5217
BbsI GAAGACnn'nnnn_
Cuts at: 1993 1993
Size: 5465
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 168 202 168
Size: 34 5431
BciVI GTATCCnnnnn_n'
Cuts at: 3363 4419 5465 3363
Size: 1056 1046 3363
Fragments arranged by size:
3363 1056 1046
BclI T'GATC_A
Cuts at: 1317 1619 2985 1317
Size: 302 1366 3797
Fragments arranged by size:
3797 1366 302
BglI GCCn_nnn'nGGC
Cuts at: 181 1526 181
Size: 1345 4120
BglII A'GATC_T
Cuts at: 2980 5017 2980
Size: 2037 3428
BlpI GC'TnA_GC
Cuts at: 1398 2577 1398
Size: 1179 4286
Bme1580I G_kGCm'C
Cuts at: 132 891 1028 1368 2139 3090 3183 4307 132
Size: 759 137 340 771 951 93 1124 1290
Fragments arranged by size:
1290 1124 951 771 759 340 137 93
BmgBI CAC'GTC
Cuts at: 2415 2415
Size: 5465
BmrI ACTGGGnnnn_n'
Cuts at: 351 822 2677 2758 3093 5237 351
Size: 471 1855 81 335 2144 579
Fragments arranged by size:
2144 1855 579 471 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 183 369 3832 183
Size: 186 3463 1816
Fragments arranged by size:
3463 1816 186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 339 562 769 1675 2472 178
Size: 161 223 207 906 797 3171
Fragments arranged by size:
3171 906 797 223 207 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 2194 2347 2641 3520 4234 4532 200
Size: 1994 153 294 879 714 298 1133
Fragments arranged by size:
1994 1133 879 714 298 294 153
BsaI GGTCTCn'nnnn_
Cuts at: 1843 5169 1843
Size: 3326 2139
BsaAI yAC'GTr
Cuts at: 264 1009 1029 2818 264
Size: 745 20 1789 2911
Fragments arranged by size:
2911 1789 745 20
BsaBI GATnn'nnATC
Cuts at: 6 2979 5016 6
Size: 2973 2037 455
Fragments arranged by size:
2973 2037 455
BsaHI Gr'CG_yC
Cuts at: 704 2896 3144 3846 5342 704
Size: 2192 248 702 1496 827
Fragments arranged by size:
2192 1496 827 702 248
BsaWI w'CCGG_w
Cuts at: 518 2654 3175 3853 4264 4411 4685 518
Size: 2136 521 678 411 147 274 1298
Fragments arranged by size:
2136 1298 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 847 877 1538 1568 847
Size: 30 661 30 4744
Fragments arranged by size:
4744 661 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 559 868 1052 1242 1275 1699 559
Size: 309 184 190 33 424 4325
Fragments arranged by size:
4325 424 309 190 184 33
BseYI C'CCAG_C
Cuts at: 848 993 1128 1375 2112 2265 2890 3648 4313
Size: 145 135 247 737 153 625 758 665
Cuts at: 4313 4721 848
Size: 408 1592
Fragments arranged by size:
1592 758 737 665 625 408 247 153
145 135
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 664 1051 1210 1228 2464 604
Size: 60 387 159 18 1236 3605
Fragments arranged by size:
3605 1236 387 159 60 18
BsiEI CG_ry'CG
Cuts at: 104 1391 1800 1901 3007 3053 4283 104
Size: 1287 409 101 1106 46 1230 1286
Fragments arranged by size:
1287 1286 1230 1106 409 101 46
BsiHKAI G_wGCw'C
Cuts at: 164 1970 2015 2336 2479 3257 3447 4307 164
Size: 1806 45 321 143 778 190 860 1322
Fragments arranged by size:
1806 1322 860 778 321 190 143 45
BsiWI C'GTAC_G
Cuts at: 1392 1392
Size: 5465
BsmI GAATG_Cn'
Cuts at: 1054 1054
Size: 5465
Bsp1286I G_dGCh'C
Cuts at: 132 142 164 625 891 1028 1368 1895 1934
Size: 10 22 461 266 137 340 527 39
Cuts at: 1934 1970 2015 2139 2336 2479 3090 3183 3257
Size: 36 45 124 197 143 611 93 74
Cuts at: 3257 3447 3509 4307 132
Size: 190 62 798 1290
Fragments arranged by size:
1290 798 611 527 461 340 266 197
190 143 137 124 93 74 62 45
39 36 22 10
BspEI T'CCGG_A
Cuts at: 518 518
Size: 5465
BspHI T'CATG_A
Cuts at: 710 2378 3897 710
Size: 1668 1519 2278
Fragments arranged by size:
2278 1668 1519
BspMI ACCTGCnnnn'nnnn_
Cuts at: 455 1801 2515 2729 3031 3412 455
Size: 1346 714 214 302 381 2508
Fragments arranged by size:
2508 1346 714 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3757 3811 5295 5460 50
Size: 3707 54 1484 165 55
Fragments arranged by size:
3707 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1415 3377 1415
Size: 1962 3503
BsrFI r'CCGG_y
Cuts at: 175 1516 3463 3644 175
Size: 1341 1947 181 1996
Fragments arranged by size:
1996 1947 1341 181
BssSI C'ACGA_G
Cuts at: 1681 3736 4444 1681
Size: 2055 708 2702
Fragments arranged by size:
2702 2055 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2842 2842
Size: 5465
BstBI TT'CG_AA
Cuts at: 1925 3826 1925
Size: 1901 3564
BstXI CCAn_nnnn'nTGG
Cuts at: 1080 1707 2187 1080
Size: 627 480 4358
Fragments arranged by size:
4358 627 480
BstYI r'GATC_y
Cuts at: 1 1064 1322 1947 2490 2957 2980 3315 3561
Size: 1063 258 625 543 467 23 335 246
Cuts at: 3561 3867 3879 3965 5017 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1063 1052 625 543 467 449 335 306
258 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 242 716 1370 1700 1853 107
Size: 135 474 654 330 153 3719
Fragments arranged by size:
3719 654 474 330 153 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 312 1083 1231 3415 3596 3759 312
Size: 771 148 2184 181 163 2018
Fragments arranged by size:
2184 2018 771 181 163 148
BtsI GCAGTG_nn'
Cuts at: 396 2037 2198 2708 396
Size: 1641 161 510 3153
Fragments arranged by size:
3153 1641 510 161
ClaI AT'CG_AT
Cuts at: 569 569
Size: 5465
DraI TTT'AAA
Cuts at: 2464 2464
Size: 5465
DraIII CAC_nnn'GTG
Cuts at: 1164 1164
Size: 5465
DrdI GACnn_nn'nnGTC
Cuts at: 3171 4515 3171
Size: 1344 4121
EaeI y'GGCC_r
Cuts at: 551 561 1070 1388 1425 1518 1877 1898 2390
Size: 10 509 318 37 93 359 21 492
Cuts at: 2390 3050 3224 3615 3642 4987 551
Size: 660 174 391 27 1345 1029
Fragments arranged by size:
1345 1029 660 509 492 391 359 318
174 93 37 27 21 10
EagI C'GGCC_G
Cuts at: 1388 1898 3050 1388
Size: 510 1152 3803
Fragments arranged by size:
3803 1152 510
EarI CTCTTCn'nnn_
Cuts at: 520 1568 1922 2069 3488 3698 520
Size: 1048 354 147 1419 210 2287
Fragments arranged by size:
2287 1419 1048 354 210 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 663 1803 3673 4415 4561 5275 663
Size: 1140 1870 742 146 714 853
Fragments arranged by size:
1870 1140 853 742 714 146
EcoICRI GAG'CTC
Cuts at: 2477 2477
Size: 5465
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 339 451 562 769 944 1675 2332 2472
Size: 161 112 111 207 175 731 657 140
Cuts at: 2472 3290 3722 4075 4737 178
Size: 818 432 353 662 906
Fragments arranged by size:
906 818 731 662 657 432 353 207
175 161 140 112 111
EcoO109I rG'GnC_Cy
Cuts at: 129 737 1043 2604 129
Size: 608 306 1561 2990
Fragments arranged by size:
2990 1561 608 306
EcoRI G'AATT_C
Cuts at: 2469 2469
Size: 5465
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 159 297 3484 159
Size: 138 3187 2140
Fragments arranged by size:
3187 2140 138
FspI TGC'GCA
Cuts at: 250 1594 3246 250
Size: 1344 1652 2469
Fragments arranged by size:
2469 1652 1344
FspAI rTGC'GCAy
Cuts at: 250 1594 250
Size: 1344 4121
HaeII r_GCGC'y
Cuts at: 2899 3147 4377 5148 2899
Size: 248 1230 771 3216
Fragments arranged by size:
3216 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 304 336 1286 1318 1939 1971 3307 3337 3339
Size: 32 950 32 621 32 1336 30 2
Cuts at: 3339 3369 304
Size: 30 2400
Fragments arranged by size:
2400 1336 950 621 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 702 2504 702
Size: 1802 3663
KasI G'GCGC_C
Cuts at: 2895 3143 2895
Size: 248 5217
KpnI G_GTAC'C
Cuts at: 777 2485 777
Size: 1708 3757
MfeI C'AATT_G
Cuts at: 932 1335 932
Size: 403 5062
MluI A'CGCG_T
Cuts at: 1548 4898 1548
Size: 3350 2115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1751 1982 2399 4224 4408 1751
Size: 231 417 1825 184 2808
Fragments arranged by size:
2808 1825 417 231 184
MscI TGG'CCA
Cuts at: 553 1072 1879 2392 3226 553
Size: 519 807 513 834 2792
Fragments arranged by size:
2792 834 807 519 513
MslI CAynn'nnrTG
Cuts at: 190 652 1009 1084 1357 1588 1705 2260 3454
Size: 462 357 75 273 231 117 555 1194
Cuts at: 3454 3581 190
Size: 127 2074
Fragments arranged by size:
2074 1194 555 462 357 273 231 127
117 75
MspA1I CmG'CkG
Cuts at: 203 993 1176 1372 1402 1820 2064 2433 2576
Size: 790 183 196 30 418 244 369 143
Cuts at: 2576 2890 3250 4034 4279 203
Size: 314 360 784 245 1389
Fragments arranged by size:
1389 790 784 418 369 360 314 245
244 196 183 143 30
NaeI GCC'GGC
Cuts at: 177 3646 177
Size: 3469 1996
NarI GG'CG_CC
Cuts at: 2896 3144 2896
Size: 248 5217
NcoI C'CATG_G
Cuts at: 107 1700 1853 107
Size: 1593 153 3719
Fragments arranged by size:
3719 1593 153
NgoMIV G'CCGG_C
Cuts at: 175 3644 175
Size: 3469 1996
NspI r_CATG'y
Cuts at: 2124 2443 2518 3018 4621 2124
Size: 319 75 500 1603 2968
Fragments arranged by size:
2968 1603 500 319 75
PciI A'CATG_T
Cuts at: 2439 4617 2439
Size: 2178 3287
PflMI CCAn_nnn'nTGG
Cuts at: 306 306
Size: 5465
PfoI T'CCnGG_A
Cuts at: 155 1652 155
Size: 1497 3968
PmeI GTTT'AAAC
Cuts at: 2464 2464
Size: 5465
PmlI CAC'GTG
Cuts at: 1009 1029 1009
Size: 20 5445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3911 3943 3911
Size: 32 5433
PpuMI rG'GwC_Cy
Cuts at: 737 1043 737
Size: 306 5159
PshAI GACnn'nnGTC
Cuts at: 1501 1501
Size: 5465
PspOMI G'GGCC_C
Cuts at: 128 128
Size: 5465
PstI C_TGCA'G
Cuts at: 455 1955 2032 2101 2512 3197 455
Size: 1500 77 69 411 685 2723
Fragments arranged by size:
2723 1500 685 411 77 69
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5465
PvuII CAG'CTG
Cuts at: 993 1402 2064 2433 2890 3250 993
Size: 409 662 369 457 360 3208
Fragments arranged by size:
3208 662 457 409 369 360
RsrII CG'GwC_CG
Cuts at: 3660 3660
Size: 5465
SacI G_AGCT'C
Cuts at: 2479 2479
Size: 5465
SacII CC_GC'GG
Cuts at: 1373 1373
Size: 5465
SalI G'TCGA_C
Cuts at: 700 2502 700
Size: 1802 3663
SanDI GG'GwC_CC
Cuts at: 1043 1043
Size: 5465
SapI GCTCTTCn'nnn_
Cuts at: 1922 3488 3698 1922
Size: 1566 210 3689
Fragments arranged by size:
3689 1566 210
SbfI CC_TGCA'GG
Cuts at: 2512 2512
Size: 5465
ScaI AGT'ACT
Cuts at: 1567 4982 1567
Size: 3415 2050
SfcI C'TryA_G
Cuts at: 33 451 1951 2028 2097 2508 3193 4161 4352
Size: 418 1500 77 69 411 685 968 191
Cuts at: 4352 33
Size: 1146
Fragments arranged by size:
1500 1146 968 685 418 411 191 77
69
SfiI GGCCn_nnn'nGGCC
Cuts at: 181 181
Size: 5465
SfoI GGC'GCC
Cuts at: 2897 3145 2897
Size: 248 5217
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5465
SmaI CCC'GGG
Cuts at: 127 1559 1672 2221 2487 127
Size: 1432 113 549 266 3105
Fragments arranged by size:
3105 1432 549 266 113
SmlI C'TyrA_G
Cuts at: 215 2209 2362 2620 3535 4249 4511 215
Size: 1994 153 258 915 714 262 1169
Fragments arranged by size:
1994 1169 915 714 262 258 153
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5465
SphI G_CATG'C
Cuts at: 2518 3018 2518
Size: 500 4965
SspI AAT'ATT
Cuts at: 909 909
Size: 5465
StuI AGG'CCT
Cuts at: 406 1639 2006 406
Size: 1233 367 3865
Fragments arranged by size:
3865 1233 367
StyI C'CwwG_G
Cuts at: 107 134 272 794 1469 1521 1700 1853 2599
Size: 27 138 522 675 52 179 153 746
Cuts at: 2599 2953 107
Size: 354 2619
Fragments arranged by size:
2619 746 675 522 354 179 153 138
52 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 804 3048 3181 3640 3849 804
Size: 2244 133 459 209 2420
Fragments arranged by size:
2420 2244 459 209 133
TatI w'GTAC_w
Cuts at: 278 1565 2396 4980 278
Size: 1287 831 2584 763
Fragments arranged by size:
2584 1287 831 763
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 847 879 4982 5014 847
Size: 32 4103 32 1298
Fragments arranged by size:
4103 1298 32 32
Tth111I GACn'n_nGTC
Cuts at: 3262 3262
Size: 5465
XbaI T'CTAG_A
Cuts at: 2496 2496
Size: 5465
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 845 2262 2960 845
Size: 1417 698 3350
Fragments arranged by size:
3350 1417 698
XmaI C'CCGG_G
Cuts at: 125 1557 1670 2219 2485 125
Size: 1432 113 549 266 3105
Fragments arranged by size:
3105 1432 549 266 113
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AscI AvrII BaeI
BbvCI BfrBI BmtI Bpu10I BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I CspCI EcoNI EcoRV FseI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PasI PsiI
PspXI PsrI SexAI SgrAI SnaBI SrfI SwaI XhoI
XmnI ZraI