Restriction map for
pF1KB5107
(Circular) (Six-base) MAPSORT of: pF1KB5107.seq Check: 7232 from: 1 to: 5318
pF1KB5107 5318 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 647 647
Size: 5318
AccI GT'mk_AC
Cuts at: 2356 2356
Size: 5318
Acc65I G'GTAC_C
Cuts at: 2334 2334
Size: 5318
AclI AA'CG_TT
Cuts at: 5158 5158
Size: 5318
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 415 952 989 1227 1460 1735 1885 2269
Size: 228 537 37 238 233 275 150 384
Cuts at: 2269 3143 3575 3928 4590 187
Size: 874 432 353 662 915
Fragments arranged by size:
915 874 662 537 432 384 353 275
238 233 228 150 37
AfeI AGC'GCT
Cuts at: 1215 1408 1215
Size: 193 5125
AflIII A'CryG_T
Cuts at: 1557 1559 2076 2670 4470 4751 1557
Size: 2 517 594 1800 281 2124
Fragments arranged by size:
2124 1800 594 517 281 2
AhdI GACnn_n'nnGTC
Cuts at: 843 2061 843
Size: 1218 4100
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 395 395
Size: 5318
AlwNI CAG_nnn'CTG
Cuts at: 960 2115 2623 4061 960
Size: 1155 508 1438 2217
Fragments arranged by size:
2217 1438 1155 508
ApaI G_GGCC'C
Cuts at: 993 1224 993
Size: 231 5087
ApaLI G'TGCA_C
Cuts at: 1702 4156 1702
Size: 2454 2864
ApoI r'AATT_y
Cuts at: 2322 4671 4927 2322
Size: 2349 256 2713
Fragments arranged by size:
2713 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5318
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5318
AvaI C'yCGr_G
Cuts at: 1756 2338 1756
Size: 582 4736
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1546 1579 1546
Size: 33 5285
BamHI G'GATC_C
Cuts at: 319 1470 2082 2343 319
Size: 1151 612 261 3294
Fragments arranged by size:
3294 1151 612 261
BanI G'GyrC_C
Cuts at: 371 1299 1851 2334 2748 2996 3031 371
Size: 928 552 483 414 248 35 2658
Fragments arranged by size:
2658 928 552 483 414 248 35
BanII G_rGCy'C
Cuts at: 134 299 883 993 1224 1390 1817 2332 3362
Size: 165 584 110 231 166 427 515 1030
Cuts at: 3362 134
Size: 2090
Fragments arranged by size:
2090 1030 584 515 427 231 166 165
110
BbeI G_GCGC'C
Cuts at: 1855 2752 3000 1855
Size: 897 248 4173
Fragments arranged by size:
4173 897 248
BbsI GAAGACnn'nnnn_
Cuts at: 327 1056 1277 327
Size: 729 221 4368
Fragments arranged by size:
4368 729 221
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5284
BciVI GTATCCnnnnn_n'
Cuts at: 3216 4272 5318 3216
Size: 1056 1046 3216
Fragments arranged by size:
3216 1056 1046
BclI T'GATC_A
Cuts at: 2838 2838
Size: 5318
BglI GCCn_nnn'nGGC
Cuts at: 619 1297 619
Size: 678 4640
BglII A'GATC_T
Cuts at: 236 2833 4870 236
Size: 2597 2037 684
Fragments arranged by size:
2597 2037 684
BlpI GC'TnA_GC
Cuts at: 1136 2105 2430 1136
Size: 969 325 4024
Fragments arranged by size:
4024 969 325
Bme1580I G_kGCm'C
Cuts at: 374 993 1224 1293 1706 2943 3036 4160 374
Size: 619 231 69 413 1237 93 1124 1532
Fragments arranged by size:
1532 1237 1124 619 413 231 93 69
BmgBI CAC'GTC
Cuts at: 255 1175 255
Size: 920 4398
BmrI ACTGGGnnnn_n'
Cuts at: 948 1955 2530 2611 2946 5090 948
Size: 1007 575 81 335 2144 1176
Fragments arranged by size:
2144 1176 1007 575 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3685 3685
Size: 5318
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1920 1920
Size: 5318
Bpu10I CC'TnA_GC
Cuts at: 730 730
Size: 5318
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 109 214 286 655 2494 3373 4087 4385 109
Size: 105 72 369 1839 879 714 298 1042
Fragments arranged by size:
1839 1042 879 714 369 298 105 72
BsaI GGTCTCn'nnnn_
Cuts at: 659 907 5022 659
Size: 248 4115 955
Fragments arranged by size:
4115 955 248
BsaAI yAC'GTr
Cuts at: 418 1362 1560 2079 2210 2671 418
Size: 944 198 519 131 461 3065
Fragments arranged by size:
3065 944 519 461 198 131
BsaBI GATnn'nnATC
Cuts at: 6 241 464 2832 4869 6
Size: 235 223 2368 2037 455
Fragments arranged by size:
2368 2037 455 235 223
BsaHI Gr'CG_yC
Cuts at: 224 1852 2749 2997 3699 5195 224
Size: 1628 897 248 702 1496 347
Fragments arranged by size:
1628 1496 897 702 347 248
BsaWI w'CCGG_w
Cuts at: 428 905 2507 3028 3706 4117 4264 4538 428
Size: 477 1602 521 678 411 147 274 1208
Fragments arranged by size:
1602 1208 678 521 477 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1310 1340 1310
Size: 30 5288
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 289 604 712 1807 289
Size: 315 108 1095 3800
Fragments arranged by size:
3800 1095 315 108
BseYI C'CCAG_C
Cuts at: 119 1210 1216 1720 2243 2276 2743 3501 4166
Size: 1091 6 504 523 33 467 758 665
Cuts at: 4166 4574 119
Size: 408 863
Fragments arranged by size:
1091 863 758 665 523 504 467 408
33 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 625 1291 1639 1669 472
Size: 153 666 348 30 4121
Fragments arranged by size:
4121 666 348 153 30
BsiEI CG_ry'CG
Cuts at: 104 2860 2906 4136 104
Size: 2756 46 1230 1286
Fragments arranged by size:
2756 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 299 967 1706 2332 3110 3300 4160 299
Size: 668 739 626 778 190 860 1457
Fragments arranged by size:
1457 860 778 739 668 626 190
BsmI GAATG_Cn'
Cuts at: 1910 1910
Size: 5318
Bsp1286I G_dGCh'C
Cuts at: 134 299 374 883 967 993 1224 1293 1390
Size: 165 75 509 84 26 231 69 97
Cuts at: 1390 1706 1817 2332 2943 3036 3110 3300 3362
Size: 316 111 515 611 93 74 190 62
Cuts at: 3362 4160 134
Size: 798 1292
Fragments arranged by size:
1292 798 611 515 509 316 231 190
165 111 97 93 84 75 74 69
62 26
BspEI T'CCGG_A
Cuts at: 428 428
Size: 5318
BspHI T'CATG_A
Cuts at: 1820 3750 1820
Size: 1930 3388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 647 2100 2368 2582 2884 3265 647
Size: 1453 268 214 302 381 2700
Fragments arranged by size:
2700 1453 381 302 268 214
BsrBI CCG'CTC
Cuts at: 50 114 141 1635 3610 3664 5148 5313 50
Size: 64 27 1494 1975 54 1484 165 55
Fragments arranged by size:
1975 1494 1484 165 64 55 54 27
BsrDI GCAATG_nn'
Cuts at: 3230 3230
Size: 5318
BsrFI r'CCGG_y
Cuts at: 1016 1258 3316 3497 1016
Size: 242 2058 181 2837
Fragments arranged by size:
2837 2058 242 181
BsrGI T'GTAC_A
Cuts at: 1919 1919
Size: 5318
BssSI C'ACGA_G
Cuts at: 751 1286 3589 4297 751
Size: 535 2303 708 1772
Fragments arranged by size:
2303 1772 708 535
BstAPI GCAn_nnn'nTGC
Cuts at: 2115 2695 2115
Size: 580 4738
BstBI TT'CG_AA
Cuts at: 3679 3679
Size: 5318
BstEII G'GTnAC_C
Cuts at: 2130 2130
Size: 5318
BstXI CCAn_nnnn'nTGG
Cuts at: 1431 1848 2058 2310 1431
Size: 417 210 252 4439
Fragments arranged by size:
4439 417 252 210
BstYI r'GATC_y
Cuts at: 1 236 319 1470 1607 2082 2343 2810 2833
Size: 235 83 1151 137 475 261 467 23
Cuts at: 2833 3168 3414 3720 3732 3818 4870 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1151 1052 475 467 449 335 306 261
246 235 137 86 83 23 12
Bsu36I CC'TnA_GG
Cuts at: 183 800 1856 183
Size: 617 1056 3645
Fragments arranged by size:
3645 1056 617
BtgI C'CryG_G
Cuts at: 1329 2125 2198 1329
Size: 796 73 4449
Fragments arranged by size:
4449 796 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3268 3449 3612 3268
Size: 181 163 4974
Fragments arranged by size:
4974 181 163
BtsI GCAGTG_nn'
Cuts at: 1136 2004 2561 1136
Size: 868 557 3893
Fragments arranged by size:
3893 868 557
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1364 1399 1364
Size: 35 5283
DraI TTT'AAA
Cuts at: 2317 2317
Size: 5318
DraIII CAC_nnn'GTG
Cuts at: 1314 1314
Size: 5318
DrdI GACnn_nn'nnGTC
Cuts at: 3024 4368 3024
Size: 1344 3974
EaeI y'GGCC_r
Cuts at: 136 611 1253 1506 2903 3077 3468 3495 4840
Size: 475 642 253 1397 174 391 27 1345
Cuts at: 4840 136
Size: 614
Fragments arranged by size:
1397 1345 642 614 475 391 253 174
27
EagI C'GGCC_G
Cuts at: 2903 2903
Size: 5318
EarI CTCTTCn'nnn_
Cuts at: 559 1475 3341 3551 559
Size: 916 1866 210 2326
Fragments arranged by size:
2326 1866 916 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3526 4268 4414 5128 3526
Size: 742 146 714 3716
Fragments arranged by size:
3716 742 714 146
EcoICRI GAG'CTC
Cuts at: 297 2330 297
Size: 2033 3285
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 415 952 989 1227 1460 1735 1885 1920
Size: 228 537 37 238 233 275 150 35
Cuts at: 1920 2269 3143 3575 3928 4590 187
Size: 349 874 432 353 662 915
Fragments arranged by size:
915 874 662 537 432 353 349 275
238 233 228 150 37 35
EcoO109I rG'GnC_Cy
Cuts at: 549 990 1220 1710 2055 2175 2457 549
Size: 441 230 490 345 120 282 3410
Fragments arranged by size:
3410 490 441 345 282 230 120
EcoRI G'AATT_C
Cuts at: 2322 2322
Size: 5318
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 217 1134 2164 3337 217
Size: 917 1030 1173 2198
Fragments arranged by size:
2198 1173 1030 917
FspI TGC'GCA
Cuts at: 1768 3099 1768
Size: 1331 3987
HaeII r_GCGC'y
Cuts at: 1217 1410 1855 1872 2097 2752 3000 4230 5001
Size: 193 445 17 225 655 248 1230 771
Cuts at: 5001 1217
Size: 1534
Fragments arranged by size:
1534 1230 771 655 445 248 225 193
17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 829 861 1159 1191 2047 2079 3160 3190 3192
Size: 32 298 32 856 32 1081 30 2
Cuts at: 3192 3222 829
Size: 30 2925
Fragments arranged by size:
2925 1081 856 298 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 810 2357 810
Size: 1547 3771
HindIII A'AGCT_T
Cuts at: 2146 2146
Size: 5318
KasI G'GCGC_C
Cuts at: 1851 2748 2996 1851
Size: 897 248 4173
Fragments arranged by size:
4173 897 248
KpnI G_GTAC'C
Cuts at: 2338 2338
Size: 5318
MfeI C'AATT_G
Cuts at: 1376 1376
Size: 5318
MluI A'CGCG_T
Cuts at: 4751 4751
Size: 5318
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4077 4261 4077
Size: 184 5134
MscI TGG'CCA
Cuts at: 1255 1508 3079 1255
Size: 253 1571 3494
Fragments arranged by size:
3494 1571 253
MslI CAynn'nnrTG
Cuts at: 377 1429 3307 3434 377
Size: 1052 1878 127 2261
Fragments arranged by size:
2261 1878 1052 127
MspA1I CmG'CkG
Cuts at: 159 1230 1797 2163 2247 2280 2429 2743 3103
Size: 1071 567 366 84 33 149 314 360
Cuts at: 3103 3887 4132 159
Size: 784 245 1345
Fragments arranged by size:
1345 1071 784 567 366 360 314 245
149 84 33
NaeI GCC'GGC
Cuts at: 3499 3499
Size: 5318
NarI GG'CG_CC
Cuts at: 1852 2749 2997 1852
Size: 897 248 4173
Fragments arranged by size:
4173 897 248
NcoI C'CATG_G
Cuts at: 1329 2125 2198 1329
Size: 796 73 4449
Fragments arranged by size:
4449 796 73
NgoMIV G'CCGG_C
Cuts at: 3497 3497
Size: 5318
NspI r_CATG'y
Cuts at: 951 1063 2371 2871 4474 951
Size: 112 1308 500 1603 1795
Fragments arranged by size:
1795 1603 1308 500 112
PciI A'CATG_T
Cuts at: 4470 4470
Size: 5318
PflMI CCAn_nnn'nTGG
Cuts at: 1082 1082
Size: 5318
PfoI T'CCnGG_A
Cuts at: 1747 1747
Size: 5318
PmeI GTTT'AAAC
Cuts at: 2317 2317
Size: 5318
PmlI CAC'GTG
Cuts at: 1560 2079 1560
Size: 519 4799
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 565 597 3764 3796 565
Size: 32 3167 32 2087
Fragments arranged by size:
3167 2087 32 32
PpuMI rG'GwC_Cy
Cuts at: 549 1710 2055 2175 549
Size: 1161 345 120 3692
Fragments arranged by size:
3692 1161 345 120
PspOMI G'GGCC_C
Cuts at: 989 1220 989
Size: 231 5087
PstI C_TGCA'G
Cuts at: 2120 2365 3050 2120
Size: 245 685 4388
Fragments arranged by size:
4388 685 245
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5318
PvuII CAG'CTG
Cuts at: 1230 1797 2163 2247 2743 3103 1230
Size: 567 366 84 496 360 3445
Fragments arranged by size:
3445 567 496 366 360 84
RsrII CG'GwC_CG
Cuts at: 3513 3513
Size: 5318
SacI G_AGCT'C
Cuts at: 299 2332 299
Size: 2033 3285
SalI G'TCGA_C
Cuts at: 2355 2355
Size: 5318
SapI GCTCTTCn'nnn_
Cuts at: 3341 3551 3341
Size: 210 5108
SbfI CC_TGCA'GG
Cuts at: 2365 2365
Size: 5318
ScaI AGT'ACT
Cuts at: 4835 4835
Size: 5318
SexAI A'CCwGG_T
Cuts at: 1340 1565 2057 1340
Size: 225 492 4601
Fragments arranged by size:
4601 492 225
SfcI C'TryA_G
Cuts at: 33 2116 2179 2361 3046 4014 4205 33
Size: 2083 63 182 685 968 191 1146
Fragments arranged by size:
2083 1146 968 685 191 182 63
SfoI GGC'GCC
Cuts at: 1853 2750 2998 1853
Size: 897 248 4173
Fragments arranged by size:
4173 897 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5318
SmaI CCC'GGG
Cuts at: 2340 2340
Size: 5318
SmlI C'TyrA_G
Cuts at: 124 229 301 670 2473 3388 4102 4364 124
Size: 105 72 369 1803 915 714 262 1078
Fragments arranged by size:
1803 1078 915 714 369 262 105 72
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5318
SphI G_CATG'C
Cuts at: 951 2371 2871 951
Size: 1420 500 3398
Fragments arranged by size:
3398 1420 500
StuI AGG'CCT
Cuts at: 763 763
Size: 5318
StyI C'CwwG_G
Cuts at: 212 1187 1329 2051 2125 2198 2452 2806 212
Size: 975 142 722 74 73 254 354 2724
Fragments arranged by size:
2724 975 722 354 254 142 74 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 485 520 1200 2149 2901 3034 3493 3702 485
Size: 35 680 949 752 133 459 209 2101
Fragments arranged by size:
2101 949 752 680 459 209 133 35
TatI w'GTAC_w
Cuts at: 788 1919 4833 788
Size: 1131 2914 1273
Fragments arranged by size:
2914 1273 1131
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1983 2015 4835 4867 1983
Size: 32 2820 32 2434
Fragments arranged by size:
2820 2434 32 32
Tth111I GACn'n_nGTC
Cuts at: 424 3115 424
Size: 2691 2627
XbaI T'CTAG_A
Cuts at: 2349 2349
Size: 5318
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 259 322 1263 2813 259
Size: 63 941 1550 2764
Fragments arranged by size:
2764 1550 941 63
XmaI C'CCGG_G
Cuts at: 2338 2338
Size: 5318
XmnI GAAnn'nnTTC
Cuts at: 1624 1624
Size: 5318
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AleI AloI AscI AvrII BbvCI
BfrBI BmtI BsiWI BsmBI BssHII BstZ17I ClaI EcoNI
EcoRV FseI FspAI HpaI NdeI NheI NotI NruI
NsiI PacI PasI PshAI PsiI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI