Restriction map for
pF1KB5108
(Circular) (Six-base) MAPSORT of: pF1KB5108.seq Check: 3036 from: 1 to: 5498
pF1KB5108 5498 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 2536 2536
Size: 5498
Acc65I G'GTAC_C
Cuts at: 128 2514 128
Size: 2386 3112
AclI AA'CG_TT
Cuts at: 5338 5338
Size: 5498
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1049 1792 1817 1853 1879 1946 3323 3755 4108
Size: 743 25 36 26 67 1377 432 353
Cuts at: 4108 4770 1049
Size: 662 1777
Fragments arranged by size:
1777 1377 743 662 432 353 67 36
26 25
AflIII A'CryG_T
Cuts at: 2850 4650 4931 2850
Size: 1800 281 3417
Fragments arranged by size:
3417 1800 281
AgeI A'CCGG_T
Cuts at: 367 367
Size: 5498
AleI CACnn'nnGTG
Cuts at: 1979 1979
Size: 5498
AlwNI CAG_nnn'CTG
Cuts at: 1597 1618 2803 4241 1597
Size: 21 1185 1438 2854
Fragments arranged by size:
2854 1438 1185 21
ApaI G_GGCC'C
Cuts at: 249 249
Size: 5498
ApaLI G'TGCA_C
Cuts at: 4336 4336
Size: 5498
ApoI r'AATT_y
Cuts at: 472 718 1306 1453 2484 2502 4851 5107 472
Size: 246 588 147 1031 18 2349 256 863
Fragments arranged by size:
2349 1031 863 588 256 246 147 18
AseI AT'TA_AT
Cuts at: 20 20
Size: 5498
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5498
AvaI C'yCGr_G
Cuts at: 221 2518 221
Size: 2297 3201
BamHI G'GATC_C
Cuts at: 2523 2523
Size: 5498
BanI G'GyrC_C
Cuts at: 128 2514 2928 3176 3211 128
Size: 2386 414 248 35 2415
Fragments arranged by size:
2415 2386 414 248 35
BanII G_rGCy'C
Cuts at: 249 1549 2019 2512 3542 249
Size: 1300 470 493 1030 2205
Fragments arranged by size:
2205 1300 1030 493 470
BbeI G_GCGC'C
Cuts at: 2932 3180 2932
Size: 248 5250
BbsI GAAGACnn'nnnn_
Cuts at: 686 686
Size: 5498
BciVI GTATCCnnnnn_n'
Cuts at: 3396 4452 5498 3396
Size: 1056 1046 3396
Fragments arranged by size:
3396 1056 1046
BclI T'GATC_A
Cuts at: 3018 3018
Size: 5498
BfrBI ATG'CAT
Cuts at: 323 392 323
Size: 69 5429
BglII A'GATC_T
Cuts at: 581 865 3013 5050 581
Size: 284 2148 2037 1029
Fragments arranged by size:
2148 2037 1029 284
BlpI GC'TnA_GC
Cuts at: 136 1109 2610 136
Size: 973 1501 3024
Fragments arranged by size:
3024 1501 973
Bme1580I G_kGCm'C
Cuts at: 249 3123 3216 4340 249
Size: 2874 93 1124 1407
Fragments arranged by size:
2874 1407 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1973 2710 2791 3126 5270 1973
Size: 737 81 335 2144 2201
Fragments arranged by size:
2201 2144 737 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3865 3865
Size: 5498
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 1855 2674 3553 4267 4565 318
Size: 1537 819 879 714 298 1251
Fragments arranged by size:
1537 1251 879 819 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 218 5202 218
Size: 4984 514
BsaAI yAC'GTr
Cuts at: 2851 2851
Size: 5498
BsaBI GATnn'nnATC
Cuts at: 6 1424 3012 5049 6
Size: 1418 1588 2037 455
Fragments arranged by size:
2037 1588 1418 455
BsaHI Gr'CG_yC
Cuts at: 2929 3177 3879 5375 2929
Size: 248 702 1496 3052
Fragments arranged by size:
3052 1496 702 248
BsaWI w'CCGG_w
Cuts at: 367 2687 3208 3886 4297 4444 4718 367
Size: 2320 521 678 411 147 274 1147
Fragments arranged by size:
2320 1147 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 194 224 1200 1230 194
Size: 30 976 30 4462
Fragments arranged by size:
4462 976 30 30
BseYI C'CCAG_C
Cuts at: 1501 2923 3681 4346 4754 1501
Size: 1422 758 665 408 2245
Fragments arranged by size:
2245 1422 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1775 1928 1775
Size: 153 5345
BsiEI CG_ry'CG
Cuts at: 104 3040 3086 4316 104
Size: 2936 46 1230 1286
Fragments arranged by size:
2936 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 900 947 2512 3290 3480 4340 900
Size: 47 1565 778 190 860 2058
Fragments arranged by size:
2058 1565 860 778 190 47
BsiWI C'GTAC_G
Cuts at: 168 168
Size: 5498
BsmI GAATG_Cn'
Cuts at: 2470 2470
Size: 5498
Bsp1286I G_dGCh'C
Cuts at: 249 900 947 1549 2019 2512 3123 3216 3290
Size: 651 47 602 470 493 611 93 74
Cuts at: 3290 3480 3542 4340 249
Size: 190 62 798 1407
Fragments arranged by size:
1407 798 651 611 602 493 470 190
93 74 62 47
BspHI T'CATG_A
Cuts at: 2132 3930 2132
Size: 1798 3700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2548 2762 3064 3445 2548
Size: 214 302 381 4601
Fragments arranged by size:
4601 381 302 214
BsrBI CCG'CTC
Cuts at: 50 116 3790 3844 5328 5493 50
Size: 66 3674 54 1484 165 55
Fragments arranged by size:
3674 1484 165 66 55 54
BsrDI GCAATG_nn'
Cuts at: 2007 2204 3410 2007
Size: 197 1206 4095
Fragments arranged by size:
4095 1206 197
BsrFI r'CCGG_y
Cuts at: 131 367 3496 3677 131
Size: 236 3129 181 1952
Fragments arranged by size:
3129 1952 236 181
BsrGI T'GTAC_A
Cuts at: 2385 2385
Size: 5498
BssSI C'ACGA_G
Cuts at: 157 3769 4477 157
Size: 3612 708 1178
Fragments arranged by size:
3612 1178 708
BstAPI GCAn_nnn'nTGC
Cuts at: 739 2875 739
Size: 2136 3362
BstBI TT'CG_AA
Cuts at: 3859 3859
Size: 5498
BstYI r'GATC_y
Cuts at: 1 581 865 1512 1555 1581 2523 2990 3013
Size: 580 284 647 43 26 942 467 23
Cuts at: 3013 3348 3594 3900 3912 3998 5050 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 942 647 580 467 449 335 306
284 246 86 43 26 23 12
BtgI C'CryG_G
Cuts at: 1637 1637
Size: 5498
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3448 3629 3792 3448
Size: 181 163 5154
Fragments arranged by size:
5154 181 163
BtsI GCAGTG_nn'
Cuts at: 506 572 2741 506
Size: 66 2169 3263
Fragments arranged by size:
3263 2169 66
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1323 1358 1323
Size: 35 5463
DraI TTT'AAA
Cuts at: 2497 2497
Size: 5498
DrdI GACnn_nn'nnGTC
Cuts at: 1166 3204 4548 1166
Size: 2038 1344 2116
Fragments arranged by size:
2116 2038 1344
EaeI y'GGCC_r
Cuts at: 294 1640 2112 3083 3257 3648 3675 5020 294
Size: 1346 472 971 174 391 27 1345 772
Fragments arranged by size:
1346 1345 971 772 472 391 174 27
EagI C'GGCC_G
Cuts at: 3083 3083
Size: 5498
EarI CTCTTCn'nnn_
Cuts at: 1554 1793 3521 3731 1554
Size: 239 1728 210 3321
Fragments arranged by size:
3321 1728 239 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 185 3706 4448 4594 5308 185
Size: 3521 742 146 714 375
Fragments arranged by size:
3521 742 714 375 146
EcoICRI GAG'CTC
Cuts at: 2510 2510
Size: 5498
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1049 1792 1817 1853 1879 1946 3323 3755 4108
Size: 743 25 36 26 67 1377 432 353
Cuts at: 4108 4770 1049
Size: 662 1777
Fragments arranged by size:
1777 1377 743 662 432 353 67 36
26 25
EcoNI CCTnn'n_nnAGG
Cuts at: 1589 1589
Size: 5498
EcoO109I rG'GnC_Cy
Cuts at: 174 246 2637 174
Size: 72 2391 3035
Fragments arranged by size:
3035 2391 72
EcoRI G'AATT_C
Cuts at: 2502 2502
Size: 5498
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3517 3517
Size: 5498
FspI TGC'GCA
Cuts at: 3279 3279
Size: 5498
HaeII r_GCGC'y
Cuts at: 2932 3180 4410 5181 2932
Size: 248 1230 771 3249
Fragments arranged by size:
3249 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 850 882 1116 1148 1833 1865 2114 2146 3340
Size: 32 234 32 685 32 249 32 1194
Cuts at: 3340 3370 3372 3402 850
Size: 30 2 30 2946
Fragments arranged by size:
2946 1194 685 249 234 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 608 1391 1811 2537 608
Size: 783 420 726 3569
Fragments arranged by size:
3569 783 726 420
HpaI GTT'AAC
Cuts at: 1391 1811 1391
Size: 420 5078
KasI G'GCGC_C
Cuts at: 2928 3176 2928
Size: 248 5250
KpnI G_GTAC'C
Cuts at: 132 2518 132
Size: 2386 3112
MfeI C'AATT_G
Cuts at: 1325 2100 1325
Size: 775 4723
MluI A'CGCG_T
Cuts at: 4931 4931
Size: 5498
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 933 4257 4441 933
Size: 3324 184 1990
Fragments arranged by size:
3324 1990 184
MscI TGG'CCA
Cuts at: 296 2114 3259 296
Size: 1818 1145 2535
Fragments arranged by size:
2535 1818 1145
MslI CAynn'nnrTG
Cuts at: 1136 1979 3487 3614 1136
Size: 843 1508 127 3020
Fragments arranged by size:
3020 1508 843 127
MspA1I CmG'CkG
Cuts at: 562 1684 2076 2609 2923 3283 4067 4312 562
Size: 1122 392 533 314 360 784 245 1748
Fragments arranged by size:
1748 1122 784 533 392 360 314 245
NaeI GCC'GGC
Cuts at: 3679 3679
Size: 5498
NarI GG'CG_CC
Cuts at: 2929 3177 2929
Size: 248 5250
NcoI C'CATG_G
Cuts at: 1637 1637
Size: 5498
NgoMIV G'CCGG_C
Cuts at: 3677 3677
Size: 5498
NsiI A_TGCA'T
Cuts at: 325 394 325
Size: 69 5429
NspI r_CATG'y
Cuts at: 2551 3051 4654 2551
Size: 500 1603 3395
Fragments arranged by size:
3395 1603 500
PciI A'CATG_T
Cuts at: 4650 4650
Size: 5498
PflMI CCAn_nnn'nTGG
Cuts at: 162 162
Size: 5498
PmeI GTTT'AAAC
Cuts at: 2497 2497
Size: 5498
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3944 3976 3944
Size: 32 5466
PspOMI G'GGCC_C
Cuts at: 245 245
Size: 5498
PstI C_TGCA'G
Cuts at: 567 2545 3230 567
Size: 1978 685 2835
Fragments arranged by size:
2835 1978 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5498
PvuII CAG'CTG
Cuts at: 562 1684 2076 2923 3283 562
Size: 1122 392 847 360 2777
Fragments arranged by size:
2777 1122 847 392 360
RsrII CG'GwC_CG
Cuts at: 3693 3693
Size: 5498
SacI G_AGCT'C
Cuts at: 2512 2512
Size: 5498
SalI G'TCGA_C
Cuts at: 2535 2535
Size: 5498
SapI GCTCTTCn'nnn_
Cuts at: 1554 3521 3731 1554
Size: 1967 210 3321
Fragments arranged by size:
3321 1967 210
SbfI CC_TGCA'GG
Cuts at: 2545 2545
Size: 5498
ScaI AGT'ACT
Cuts at: 5015 5015
Size: 5498
SexAI A'CCwGG_T
Cuts at: 124 1143 1611 124
Size: 1019 468 4011
Fragments arranged by size:
4011 1019 468
SfcI C'TryA_G
Cuts at: 33 563 2469 2541 3226 4194 4385 33
Size: 530 1906 72 685 968 191 1146
Fragments arranged by size:
1906 1146 968 685 530 191 72
SfoI GGC'GCC
Cuts at: 2930 3178 2930
Size: 248 5250
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5498
SgrAI Cr'CCGG_yG
Cuts at: 367 367
Size: 5498
SmaI CCC'GGG
Cuts at: 2520 2520
Size: 5498
SmlI C'TyrA_G
Cuts at: 333 1834 2653 3568 4282 4544 333
Size: 1501 819 915 714 262 1287
Fragments arranged by size:
1501 1287 915 819 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5498
SphI G_CATG'C
Cuts at: 2551 3051 2551
Size: 500 4998
SspI AAT'ATT
Cuts at: 1704 1760 1704
Size: 56 5442
StyI C'CwwG_G
Cuts at: 1384 1637 2632 2986 1384
Size: 253 995 354 3896
Fragments arranged by size:
3896 995 354 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3081 3214 3673 3882 3081
Size: 133 459 209 4697
Fragments arranged by size:
4697 459 209 133
TatI w'GTAC_w
Cuts at: 2313 2385 5013 2313
Size: 72 2628 2798
Fragments arranged by size:
2798 2628 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 194 226 1200 1232 1566 1598 5015 5047 194
Size: 32 974 32 334 32 3417 32 645
Fragments arranged by size:
3417 974 645 334 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3295 3295
Size: 5498
XbaI T'CTAG_A
Cuts at: 2529 2529
Size: 5498
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2993 2993
Size: 5498
XmaI C'CCGG_G
Cuts at: 2518 2518
Size: 5498
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI BpmI
Bpu10I BseRI BsmBI BspEI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI DraIII EcoRV FseI FspAI HindIII NdeI
NheI NotI NruI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SfiI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI