Restriction map for
pF1KB5109
(Circular) (Six-base) MAPSORT of: pF1KB5109.seq Check: 591 from: 1 to: 4931
pF1KB5109 4931 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 138 989 138
Size: 851 4080
AatII G_ACGT'C
Cuts at: 896 896
Size: 4931
AccI GT'mk_AC
Cuts at: 1969 1969
Size: 4931
Acc65I G'GTAC_C
Cuts at: 1947 1947
Size: 4931
AclI AA'CG_TT
Cuts at: 4771 4771
Size: 4931
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 461 1407 1423 1564 2756 3188 3541 4203 461
Size: 946 16 141 1192 432 353 662 1189
Fragments arranged by size:
1192 1189 946 662 432 353 141 16
AflIII A'CryG_T
Cuts at: 2283 4083 4364 2283
Size: 1800 281 2850
Fragments arranged by size:
2850 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1393 1393
Size: 4931
AleI CACnn'nnGTG
Cuts at: 809 809
Size: 4931
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 868 1060 868
Size: 192 4739
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1195 1227 1195
Size: 32 4899
AlwNI CAG_nnn'CTG
Cuts at: 954 1083 2236 3674 954
Size: 129 1153 1438 2211
Fragments arranged by size:
2211 1438 1153 129
ApaI G_GGCC'C
Cuts at: 1579 1579
Size: 4931
ApaLI G'TGCA_C
Cuts at: 1465 3769 1465
Size: 2304 2627
ApoI r'AATT_y
Cuts at: 1935 4284 4540 1935
Size: 2349 256 2326
Fragments arranged by size:
2349 2326 256
AseI AT'TA_AT
Cuts at: 20 1145 20
Size: 1125 3806
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4931
AvaI C'yCGr_G
Cuts at: 1357 1951 1357
Size: 594 4337
BamHI G'GATC_C
Cuts at: 1956 1956
Size: 4931
BanI G'GyrC_C
Cuts at: 1534 1609 1947 2361 2609 2644 1534
Size: 75 338 414 248 35 3821
Fragments arranged by size:
3821 414 338 248 75 35
BanII G_rGCy'C
Cuts at: 934 1451 1579 1945 2975 934
Size: 517 128 366 1030 2890
Fragments arranged by size:
2890 1030 517 366 128
BbeI G_GCGC'C
Cuts at: 1613 2365 2613 1613
Size: 752 248 3931
Fragments arranged by size:
3931 752 248
BbsI GAAGACnn'nnnn_
Cuts at: 449 489 769 449
Size: 40 280 4611
Fragments arranged by size:
4611 280 40
BbvCI CC'TCA_GC
Cuts at: 352 352
Size: 4931
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4897
BciVI GTATCCnnnnn_n'
Cuts at: 357 1357 2829 3885 4931 357
Size: 1000 1472 1056 1046 357
Fragments arranged by size:
1472 1056 1046 1000 357
BclI T'GATC_A
Cuts at: 2451 2451
Size: 4931
BglI GCCn_nnn'nGGC
Cuts at: 588 588
Size: 4931
BglII A'GATC_T
Cuts at: 2446 4483 2446
Size: 2037 2894
BlpI GC'TnA_GC
Cuts at: 2043 2043
Size: 4931
Bme1580I G_kGCm'C
Cuts at: 1469 1537 1579 2556 2649 3773 1469
Size: 68 42 977 93 1124 2627
Fragments arranged by size:
2627 1124 977 93 68 42
BmgBI CAC'GTC
Cuts at: 1050 1590 1050
Size: 540 4391
BmrI ACTGGGnnnn_n'
Cuts at: 776 1161 2143 2224 2559 4703 776
Size: 385 982 81 335 2144 1004
Fragments arranged by size:
2144 1004 982 385 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1194 3298 1194
Size: 2104 2827
Bpu10I CC'TnA_GC
Cuts at: 352 352
Size: 4931
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2107 2986 3700 3998 2107
Size: 879 714 298 3040
Fragments arranged by size:
3040 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4635 4635
Size: 4931
BsaAI yAC'GTr
Cuts at: 2284 2284
Size: 4931
BsaBI GATnn'nnATC
Cuts at: 6 748 2445 4482 6
Size: 742 1697 2037 455
Fragments arranged by size:
2037 1697 742 455
BsaHI Gr'CG_yC
Cuts at: 161 893 1610 1631 2362 2610 3312 4808 161
Size: 732 717 21 731 248 702 1496 284
Fragments arranged by size:
1496 732 731 717 702 284 248 21
BsaWI w'CCGG_w
Cuts at: 331 1753 2120 2641 3319 3730 3877 4151 331
Size: 1422 367 521 678 411 147 274 1111
Fragments arranged by size:
1422 1111 678 521 411 367 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 313 343 370 400 763 793 1735 1765 313
Size: 30 27 30 363 30 942 30 3479
Fragments arranged by size:
3479 942 363 30 30 30 30 27
BseYI C'CCAG_C
Cuts at: 249 310 412 786 954 1033 1186 2356 3114
Size: 61 102 374 168 79 153 1170 758
Cuts at: 3114 3779 4187 249
Size: 665 408 993
Fragments arranged by size:
1170 993 758 665 408 374 168 153
102 79 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1030 1819 1030
Size: 789 4142
BsiEI CG_ry'CG
Cuts at: 104 2473 2519 3749 104
Size: 2369 46 1230 1286
Fragments arranged by size:
2369 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 278 383 1469 1871 1945 2723 2913 3773 278
Size: 105 1086 402 74 778 190 860 1436
Fragments arranged by size:
1436 1086 860 778 402 190 105 74
BsiWI C'GTAC_G
Cuts at: 113 113
Size: 4931
BsmI GAATG_Cn'
Cuts at: 622 622
Size: 4931
BsmBI CGTCTCn'nnnn_
Cuts at: 168 1156 168
Size: 988 3943
Bsp1286I G_dGCh'C
Cuts at: 278 383 934 1451 1469 1537 1579 1871 1945
Size: 105 551 517 18 68 42 292 74
Cuts at: 1945 2556 2649 2723 2913 2975 3773 278
Size: 611 93 74 190 62 798 1436
Fragments arranged by size:
1436 798 611 551 517 292 190 105
93 74 74 68 62 42 18
BspEI T'CCGG_A
Cuts at: 331 331
Size: 4931
BspHI T'CATG_A
Cuts at: 3363 3363
Size: 4931
BspMI ACCTGCnnnn'nnnn_
Cuts at: 138 989 1981 2195 2497 2878 138
Size: 851 992 214 302 381 2191
Fragments arranged by size:
2191 992 851 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3223 3277 4761 4926 50
Size: 3173 54 1484 165 55
Fragments arranged by size:
3173 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 323 428 2843 323
Size: 105 2415 2411
Fragments arranged by size:
2415 2411 105
BsrFI r'CCGG_y
Cuts at: 578 587 1829 2929 3110 578
Size: 9 1242 1100 181 2399
Fragments arranged by size:
2399 1242 1100 181 9
BsrGI T'GTAC_A
Cuts at: 206 206
Size: 4931
BssHII G'CGCG_C
Cuts at: 782 782
Size: 4931
BssSI C'ACGA_G
Cuts at: 3202 3910 3202
Size: 708 4223
BstAPI GCAn_nnn'nTGC
Cuts at: 2308 2308
Size: 4931
BstBI TT'CG_AA
Cuts at: 3292 3292
Size: 4931
BstXI CCAn_nnnn'nTGG
Cuts at: 811 942 1641 811
Size: 131 699 4101
Fragments arranged by size:
4101 699 131
BstYI r'GATC_y
Cuts at: 1 356 1814 1956 2423 2446 2781 3027 3333
Size: 355 1458 142 467 23 335 246 306
Cuts at: 3333 3345 3431 4483 1
Size: 12 86 1052 449
Fragments arranged by size:
1458 1052 467 449 355 335 306 246
142 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 188 232 188
Size: 44 4887
BtgI C'CryG_G
Cuts at: 461 596 908 926 935 1223 1370 461
Size: 135 312 18 9 288 147 4022
Fragments arranged by size:
4022 312 288 147 135 18 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 620 2881 3062 3225 620
Size: 2261 181 163 2326
Fragments arranged by size:
2326 2261 181 163
BtsI GCAGTG_nn'
Cuts at: 1469 2174 1469
Size: 705 4226
ClaI AT'CG_AT
Cuts at: 1072 1072
Size: 4931
DraI TTT'AAA
Cuts at: 1930 1930
Size: 4931
DrdI GACnn_nn'nnGTC
Cuts at: 1510 2637 3981 1510
Size: 1127 1344 2460
Fragments arranged by size:
2460 1344 1127
EaeI y'GGCC_r
Cuts at: 296 580 593 738 2516 2690 3081 3108 4453
Size: 284 13 145 1778 174 391 27 1345
Cuts at: 4453 296
Size: 774
Fragments arranged by size:
1778 1345 774 391 284 174 145 27
13
EagI C'GGCC_G
Cuts at: 2516 2516
Size: 4931
EarI CTCTTCn'nnn_
Cuts at: 522 2954 3164 522
Size: 2432 210 2289
Fragments arranged by size:
2432 2289 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 806 1504 3139 3881 4027 4741 806
Size: 698 1635 742 146 714 996
Fragments arranged by size:
1635 996 742 714 698 146
EcoICRI GAG'CTC
Cuts at: 1943 1943
Size: 4931
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 461 1407 1423 1564 2756 3188 3541 4203 461
Size: 946 16 141 1192 432 353 662 1189
Fragments arranged by size:
1192 1189 946 662 432 353 141 16
EcoNI CCTnn'n_nnAGG
Cuts at: 145 1402 145
Size: 1257 3674
EcoO109I rG'GnC_Cy
Cuts at: 634 1575 1576 2070 634
Size: 941 1 494 3495
Fragments arranged by size:
3495 941 494 1
EcoRI G'AATT_C
Cuts at: 1935 1935
Size: 4931
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2950 2950
Size: 4931
FspI TGC'GCA
Cuts at: 2712 2712
Size: 4931
HaeII r_GCGC'y
Cuts at: 1613 2365 2613 3843 4614 1613
Size: 752 248 1230 771 1930
Fragments arranged by size:
1930 1230 771 752 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 499 531 1065 1097 1379 1411 1657 1689 1799
Size: 32 534 32 282 32 246 32 110
Cuts at: 1799 1831 2773 2803 2805 2835 499
Size: 32 942 30 2 30 2595
Fragments arranged by size:
2595 942 534 282 246 110 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 684 1970 684
Size: 1286 3645
KasI G'GCGC_C
Cuts at: 1609 2361 2609 1609
Size: 752 248 3931
Fragments arranged by size:
3931 752 248
KpnI G_GTAC'C
Cuts at: 1951 1951
Size: 4931
MfeI C'AATT_G
Cuts at: 1490 1490
Size: 4931
MluI A'CGCG_T
Cuts at: 4364 4364
Size: 4931
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 811 3690 3874 811
Size: 2879 184 1868
Fragments arranged by size:
2879 1868 184
MscI TGG'CCA
Cuts at: 298 595 2692 298
Size: 297 2097 2537
Fragments arranged by size:
2537 2097 297
MslI CAynn'nnrTG
Cuts at: 809 1765 1771 2920 3047 809
Size: 956 6 1149 127 2693
Fragments arranged by size:
2693 1149 956 127 6
MspA1I CmG'CkG
Cuts at: 314 954 1250 1304 2042 2356 2716 3500 3745
Size: 640 296 54 738 314 360 784 245
Cuts at: 3745 314
Size: 1500
Fragments arranged by size:
1500 784 738 640 360 314 296 245
54
NaeI GCC'GGC
Cuts at: 580 3112 580
Size: 2532 2399
NarI GG'CG_CC
Cuts at: 1610 2362 2610 1610
Size: 752 248 3931
Fragments arranged by size:
3931 752 248
NcoI C'CATG_G
Cuts at: 926 926
Size: 4931
NgoMIV G'CCGG_C
Cuts at: 578 3110 578
Size: 2532 2399
NspI r_CATG'y
Cuts at: 1984 2484 4087 1984
Size: 500 1603 2828
Fragments arranged by size:
2828 1603 500
PciI A'CATG_T
Cuts at: 4083 4083
Size: 4931
PflMI CCAn_nnn'nTGG
Cuts at: 288 887 954 288
Size: 599 67 4265
Fragments arranged by size:
4265 599 67
PfoI T'CCnGG_A
Cuts at: 1213 1213
Size: 4931
PmeI GTTT'AAAC
Cuts at: 1930 1930
Size: 4931
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3377 3409 3377
Size: 32 4899
PpuMI rG'GwC_Cy
Cuts at: 634 634
Size: 4931
PspOMI G'GGCC_C
Cuts at: 1575 1575
Size: 4931
PstI C_TGCA'G
Cuts at: 1255 1978 2663 1255
Size: 723 685 3523
Fragments arranged by size:
3523 723 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4931
PvuII CAG'CTG
Cuts at: 954 1250 2356 2716 954
Size: 296 1106 360 3169
Fragments arranged by size:
3169 1106 360 296
RsrII CG'GwC_CG
Cuts at: 3126 3126
Size: 4931
SacI G_AGCT'C
Cuts at: 1945 1945
Size: 4931
SalI G'TCGA_C
Cuts at: 1968 1968
Size: 4931
SanDI GG'GwC_CC
Cuts at: 634 634
Size: 4931
SapI GCTCTTCn'nnn_
Cuts at: 522 2954 3164 522
Size: 2432 210 2289
Fragments arranged by size:
2432 2289 210
SbfI CC_TGCA'GG
Cuts at: 1978 1978
Size: 4931
ScaI AGT'ACT
Cuts at: 4448 4448
Size: 4931
SexAI A'CCwGG_T
Cuts at: 1205 1205
Size: 4931
SfcI C'TryA_G
Cuts at: 33 1251 1299 1974 2659 3627 3818 33
Size: 1218 48 675 685 968 191 1146
Fragments arranged by size:
1218 1146 968 685 675 191 48
SfoI GGC'GCC
Cuts at: 1611 2363 2611 1611
Size: 752 248 3931
Fragments arranged by size:
3931 752 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4931
SmaI CCC'GGG
Cuts at: 1359 1953 1359
Size: 594 4337
SmlI C'TyrA_G
Cuts at: 2086 3001 3715 3977 2086
Size: 915 714 262 3040
Fragments arranged by size:
3040 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4931
SphI G_CATG'C
Cuts at: 1984 2484 1984
Size: 500 4431
StyI C'CwwG_G
Cuts at: 926 1571 2065 2419 926
Size: 645 494 354 3438
Fragments arranged by size:
3438 645 494 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 862 1600 2514 2647 3106 3315 862
Size: 738 914 133 459 209 2478
Fragments arranged by size:
2478 914 738 459 209 133
TatI w'GTAC_w
Cuts at: 206 4446 206
Size: 4240 691
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 763 795 1355 1387 1735 1767 1776 1808 4448
Size: 32 560 32 348 32 9 32 2640
Cuts at: 4448 4480 763
Size: 32 1214
Fragments arranged by size:
2640 1214 560 348 32 32 32 32
32 9
Tth111I GACn'n_nGTC
Cuts at: 2728 2728
Size: 4931
XbaI T'CTAG_A
Cuts at: 1962 1962
Size: 4931
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 409 1221 2426 409
Size: 812 1205 2914
Fragments arranged by size:
2914 1205 812
XmaI C'CCGG_G
Cuts at: 1357 1951 1357
Size: 594 4337
XmnI GAAnn'nnTTC
Cuts at: 130 1420 130
Size: 1290 3641
ZraI GAC'GTC
Cuts at: 894 894
Size: 4931
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AscI AvrII BaeI BfrBI BmtI
BpmI BseRI BstEII BstZ17I CspCI DraIII EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PspXI PsrI SacII
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI