Restriction map for
pF1KB5110
(Circular) (Six-base) MAPSORT of: pF1KB5110.seq Check: 9773 from: 1 to: 5027
pF1KB5110 5027 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 344 1316 2065 344
Size: 972 749 3306
Fragments arranged by size:
3306 972 749
Acc65I G'GTAC_C
Cuts at: 2043 2043
Size: 5027
AclI AA'CG_TT
Cuts at: 4867 4867
Size: 5027
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 961 1099 1431 2852 3284 3637 4299 961
Size: 138 332 1421 432 353 662 1689
Fragments arranged by size:
1689 1421 662 432 353 332 138
AflIII A'CryG_T
Cuts at: 197 2379 4179 4460 197
Size: 2182 1800 281 764
Fragments arranged by size:
2182 1800 764 281
AleI CACnn'nnGTG
Cuts at: 1564 1564
Size: 5027
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1582 1706 1582
Size: 124 4903
AlwNI CAG_nnn'CTG
Cuts at: 670 984 1296 2332 3770 670
Size: 314 312 1036 1438 1927
Fragments arranged by size:
1927 1438 1036 314 312
ApaLI G'TGCA_C
Cuts at: 3865 3865
Size: 5027
ApoI r'AATT_y
Cuts at: 520 1873 2031 4380 4636 520
Size: 1353 158 2349 256 911
Fragments arranged by size:
2349 1353 911 256 158
AseI AT'TA_AT
Cuts at: 20 20
Size: 5027
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5027
AvaI C'yCGr_G
Cuts at: 842 2047 842
Size: 1205 3822
BamHI G'GATC_C
Cuts at: 2052 2052
Size: 5027
BanI G'GyrC_C
Cuts at: 1951 2043 2457 2705 2740 1951
Size: 92 414 248 35 4238
Fragments arranged by size:
4238 414 248 92 35
BanII G_rGCy'C
Cuts at: 2041 3071 2041
Size: 1030 3997
BbeI G_GCGC'C
Cuts at: 2461 2709 2461
Size: 248 4779
BbsI GAAGACnn'nnnn_
Cuts at: 679 1121 679
Size: 442 4585
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 997 1031 1920 1954 90
Size: 34 873 34 889 34 3163
Fragments arranged by size:
3163 889 873 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2925 3981 5027 2925
Size: 1056 1046 2925
Fragments arranged by size:
2925 1056 1046
BclI T'GATC_A
Cuts at: 1146 2547 1146
Size: 1401 3626
BglII A'GATC_T
Cuts at: 2542 4579 2542
Size: 2037 2990
BlpI GC'TnA_GC
Cuts at: 2139 2139
Size: 5027
Bme1580I G_kGCm'C
Cuts at: 1530 1954 2652 2745 3869 1530
Size: 424 698 93 1124 2688
Fragments arranged by size:
2688 1124 698 424 93
BmgBI CAC'GTC
Cuts at: 363 1653 363
Size: 1290 3737
BmrI ACTGGGnnnn_n'
Cuts at: 865 968 1066 2239 2320 2655 4799 865
Size: 103 98 1173 81 335 2144 1093
Fragments arranged by size:
2144 1173 1093 335 103 98 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3394 3394
Size: 5027
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1523 1523
Size: 5027
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 1125 2203 3082 3796 4094 253
Size: 872 1078 879 714 298 1186
Fragments arranged by size:
1186 1078 879 872 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4731 4731
Size: 5027
BsaAI yAC'GTr
Cuts at: 1636 2380 1636
Size: 744 4283
BsaBI GATnn'nnATC
Cuts at: 6 2541 4578 6
Size: 2535 2037 455
Fragments arranged by size:
2535 2037 455
BsaHI Gr'CG_yC
Cuts at: 2458 2706 3408 4904 2458
Size: 248 702 1496 2581
Fragments arranged by size:
2581 1496 702 248
BsaWI w'CCGG_w
Cuts at: 184 2216 2737 3415 3826 3973 4247 184
Size: 2032 521 678 411 147 274 964
Fragments arranged by size:
2032 964 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 496 742 766 496
Size: 246 24 4757
Fragments arranged by size:
4757 246 24
BseYI C'CCAG_C
Cuts at: 1343 1483 2452 3210 3875 4283 1343
Size: 140 969 758 665 408 2087
Fragments arranged by size:
2087 969 758 665 408 140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1309 1588 1309
Size: 279 4748
BsiEI CG_ry'CG
Cuts at: 104 2569 2615 3845 104
Size: 2465 46 1230 1286
Fragments arranged by size:
2465 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 152 504 2041 2819 3009 3869 152
Size: 352 1537 778 190 860 1310
Fragments arranged by size:
1537 1310 860 778 352 190
Bsp1286I G_dGCh'C
Cuts at: 152 504 1530 1954 2041 2652 2745 2819 3009
Size: 352 1026 424 87 611 93 74 190
Cuts at: 3009 3071 3869 152
Size: 62 798 1310
Fragments arranged by size:
1310 1026 798 611 424 352 190 93
87 74 62
BspHI T'CATG_A
Cuts at: 2012 3459 2012
Size: 1447 3580
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2077 2291 2593 2974 2077
Size: 214 302 381 4130
Fragments arranged by size:
4130 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3319 3373 4857 5022 50
Size: 3269 54 1484 165 55
Fragments arranged by size:
3269 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1154 1178 1457 1607 1622 1782 2939 1154
Size: 24 279 150 15 160 1157 3242
Fragments arranged by size:
3242 1157 279 160 150 24 15
BsrFI r'CCGG_y
Cuts at: 1954 3025 3206 1954
Size: 1071 181 3775
Fragments arranged by size:
3775 1071 181
BssSI C'ACGA_G
Cuts at: 3298 4006 3298
Size: 708 4319
BstAPI GCAn_nnn'nTGC
Cuts at: 1180 1296 2404 1180
Size: 116 1108 3803
Fragments arranged by size:
3803 1108 116
BstBI TT'CG_AA
Cuts at: 3388 3388
Size: 5027
BstXI CCAn_nnnn'nTGG
Cuts at: 1215 1998 1215
Size: 783 4244
BstYI r'GATC_y
Cuts at: 1 323 398 494 981 2052 2519 2542 2877
Size: 322 75 96 487 1071 467 23 335
Cuts at: 2877 3123 3429 3441 3527 4579 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1071 1052 487 467 449 335 322 306
246 96 86 75 23 12
Bsu36I CC'TnA_GG
Cuts at: 917 917
Size: 5027
BtgI C'CryG_G
Cuts at: 1490 1562 1613 1784 1490
Size: 72 51 171 4733
Fragments arranged by size:
4733 171 72 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2977 3158 3321 2977
Size: 181 163 4683
Fragments arranged by size:
4683 181 163
BtsI GCAGTG_nn'
Cuts at: 2270 2270
Size: 5027
DraI TTT'AAA
Cuts at: 2026 2026
Size: 5027
DraIII CAC_nnn'GTG
Cuts at: 1060 1060
Size: 5027
DrdI GACnn_nn'nnGTC
Cuts at: 1183 1795 2733 4077 1183
Size: 612 938 1344 2133
Fragments arranged by size:
2133 1344 938 612
EaeI y'GGCC_r
Cuts at: 1554 1712 1956 2612 2786 3177 3204 4549 1554
Size: 158 244 656 174 391 27 1345 2032
Fragments arranged by size:
2032 1345 656 391 244 174 158 27
EagI C'GGCC_G
Cuts at: 2612 2612
Size: 5027
EarI CTCTTCn'nnn_
Cuts at: 3050 3260 3050
Size: 210 4817
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3235 3977 4123 4837 3235
Size: 742 146 714 3425
Fragments arranged by size:
3425 742 714 146
EcoICRI GAG'CTC
Cuts at: 2039 2039
Size: 5027
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 961 1099 1431 1523 2852 3284 3637 4299 961
Size: 138 332 92 1329 432 353 662 1689
Fragments arranged by size:
1689 1329 662 432 353 332 138 92
EcoNI CCTnn'n_nnAGG
Cuts at: 2014 2014
Size: 5027
EcoO109I rG'GnC_Cy
Cuts at: 697 1083 2166 697
Size: 386 1083 3558
Fragments arranged by size:
3558 1083 386
EcoRI G'AATT_C
Cuts at: 2031 2031
Size: 5027
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1440 3046 1440
Size: 1606 3421
FspI TGC'GCA
Cuts at: 2808 2808
Size: 5027
HaeII r_GCGC'y
Cuts at: 1359 2461 2709 3939 4710 1359
Size: 1102 248 1230 771 1676
Fragments arranged by size:
1676 1230 1102 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 486 518 1092 1108 1124 1131 1140 1163 2869
Size: 32 574 16 16 7 9 23 1706
Cuts at: 2869 2899 2901 2931 486
Size: 30 2 30 2582
Fragments arranged by size:
2582 1706 574 32 30 30 23 16
16 9 7 2
HincII GTy'rAC
Cuts at: 195 2066 195
Size: 1871 3156
HpaI GTT'AAC
Cuts at: 195 195
Size: 5027
KasI G'GCGC_C
Cuts at: 2457 2705 2457
Size: 248 4779
KpnI G_GTAC'C
Cuts at: 2047 2047
Size: 5027
MluI A'CGCG_T
Cuts at: 197 4460 197
Size: 4263 764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 426 815 863 1196 3786 3970 426
Size: 389 48 333 2590 184 1483
Fragments arranged by size:
2590 1483 389 333 184 48
MscI TGG'CCA
Cuts at: 1714 2788 1714
Size: 1074 3953
MslI CAynn'nnrTG
Cuts at: 511 1564 1612 3016 3143 511
Size: 1053 48 1404 127 2395
Fragments arranged by size:
2395 1404 1053 127 48
MspA1I CmG'CkG
Cuts at: 1500 1794 2138 2452 2812 3596 3841 1500
Size: 294 344 314 360 784 245 2686
Fragments arranged by size:
2686 784 360 344 314 294 245
NaeI GCC'GGC
Cuts at: 3208 3208
Size: 5027
NarI GG'CG_CC
Cuts at: 2458 2706 2458
Size: 248 4779
NcoI C'CATG_G
Cuts at: 1562 1613 1562
Size: 51 4976
NdeI CA'TA_TG
Cuts at: 339 339
Size: 5027
NgoMIV G'CCGG_C
Cuts at: 3206 3206
Size: 5027
NspI r_CATG'y
Cuts at: 1068 1323 1683 2080 2580 4183 1068
Size: 255 360 397 500 1603 1912
Fragments arranged by size:
1912 1603 500 397 360 255
PciI A'CATG_T
Cuts at: 4179 4179
Size: 5027
PflMI CCAn_nnn'nTGG
Cuts at: 1830 1830
Size: 5027
PfoI T'CCnGG_A
Cuts at: 1325 1325
Size: 5027
PmeI GTTT'AAAC
Cuts at: 2026 2026
Size: 5027
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3473 3505 3473
Size: 32 4995
PpuMI rG'GwC_Cy
Cuts at: 697 697
Size: 5027
PstI C_TGCA'G
Cuts at: 224 675 994 1176 1301 2074 2759 224
Size: 451 319 182 125 773 685 2492
Fragments arranged by size:
2492 773 685 451 319 182 125
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5027
PvuII CAG'CTG
Cuts at: 1500 1794 2452 2812 1500
Size: 294 658 360 3715
Fragments arranged by size:
3715 658 360 294
RsrII CG'GwC_CG
Cuts at: 3222 3222
Size: 5027
SacI G_AGCT'C
Cuts at: 2041 2041
Size: 5027
SalI G'TCGA_C
Cuts at: 2064 2064
Size: 5027
SapI GCTCTTCn'nnn_
Cuts at: 3050 3260 3050
Size: 210 4817
SbfI CC_TGCA'GG
Cuts at: 2074 2074
Size: 5027
ScaI AGT'ACT
Cuts at: 894 4544 894
Size: 3650 1377
SfcI C'TryA_G
Cuts at: 33 220 671 990 1172 1273 1297 2070 2755
Size: 187 451 319 182 101 24 773 685
Cuts at: 2755 3723 3914 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 773 685 451 319 191 187
182 101 24
SfoI GGC'GCC
Cuts at: 2459 2707 2459
Size: 248 4779
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5027
SmaI CCC'GGG
Cuts at: 844 2049 844
Size: 1205 3822
SmlI C'TyrA_G
Cuts at: 268 1104 2182 3097 3811 4073 268
Size: 836 1078 915 714 262 1222
Fragments arranged by size:
1222 1078 915 836 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5027
SphI G_CATG'C
Cuts at: 1683 2080 2580 1683
Size: 397 500 4130
Fragments arranged by size:
4130 500 397
StyI C'CwwG_G
Cuts at: 134 587 1046 1562 1613 1641 1814 1991 2161
Size: 453 459 516 51 28 173 177 170
Cuts at: 2161 2515 134
Size: 354 2646
Fragments arranged by size:
2646 516 459 453 354 177 173 170
51 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 265 847 1048 2610 2743 3202 3411 265
Size: 582 201 1562 133 459 209 1881
Fragments arranged by size:
1881 1562 582 459 209 201 133
TatI w'GTAC_w
Cuts at: 892 4542 892
Size: 3650 1377
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4544 4576 4544
Size: 32 4995
Tth111I GACn'n_nGTC
Cuts at: 960 2824 960
Size: 1864 3163
XbaI T'CTAG_A
Cuts at: 2058 2058
Size: 5027
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1053 1944 1998 2522 1053
Size: 891 54 524 3558
Fragments arranged by size:
3558 891 524 54
XmaI C'CCGG_G
Cuts at: 842 2047 842
Size: 1205 3822
XmnI GAAnn'nnTTC
Cuts at: 1444 1444
Size: 5027
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmtI Bpu10I
BsaXI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII
BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII MfeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI