Restriction map for
pF1KB5115
(Circular) (Six-base) MAPSORT of: pF1KB5115.seq Check: 7257 from: 1 to: 6404
pF1KB5115 6404 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2262 2262
Size: 6404
AccI GT'mk_AC
Cuts at: 848 932 3442 848
Size: 84 2510 3810
Fragments arranged by size:
3810 2510 84
Acc65I G'GTAC_C
Cuts at: 3420 3420
Size: 6404
AclI AA'CG_TT
Cuts at: 6244 6244
Size: 6404
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 638 2773 3020 4229 4661 5408 5676 638
Size: 2135 247 1209 432 747 268 1366
Fragments arranged by size:
2135 1366 1209 747 432 268 247
AflIII A'CryG_T
Cuts at: 194 937 1242 1254 3173 3756 4860 5837 194
Size: 743 305 12 1919 583 1104 977 761
Fragments arranged by size:
1919 1104 977 761 743 583 305 12
AgeI A'CCGG_T
Cuts at: 4792 4792
Size: 6404
AhdI GACnn_n'nnGTC
Cuts at: 3174 3174
Size: 6404
AleI CACnn'nnGTG
Cuts at: 877 877
Size: 6404
AlwNI CAG_nnn'CTG
Cuts at: 293 616 3709 5276 293
Size: 323 3093 1567 1421
Fragments arranged by size:
3093 1567 1421 323
ApaI G_GGCC'C
Cuts at: 751 1982 751
Size: 1231 5173
ApaLI G'TGCA_C
Cuts at: 5174 5174
Size: 6404
ApoI r'AATT_y
Cuts at: 661 1642 2718 2994 3408 5757 6013 661
Size: 981 1076 276 414 2349 256 1052
Fragments arranged by size:
2349 1076 1052 981 414 276 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6404
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6404
AvaI C'yCGr_G
Cuts at: 3424 3424
Size: 6404
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 840 873 840
Size: 33 6371
BamHI G'GATC_C
Cuts at: 3429 3429
Size: 6404
BanI G'GyrC_C
Cuts at: 2029 2137 3420 3834 4082 4117 2029
Size: 108 1283 414 248 35 4316
Fragments arranged by size:
4316 1283 414 248 108 35
BanII G_rGCy'C
Cuts at: 568 751 1982 3213 3418 4448 568
Size: 183 1231 1231 205 1030 2524
Fragments arranged by size:
2524 1231 1231 1030 205 183
BbeI G_GCGC'C
Cuts at: 3838 4086 3838
Size: 248 6156
BbsI GAAGACnn'nnnn_
Cuts at: 125 577 1060 125
Size: 452 483 5469
Fragments arranged by size:
5469 483 452
BbvCI CC'TCA_GC
Cuts at: 239 470 239
Size: 231 6173
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3193 3227 3193
Size: 34 6370
BciVI GTATCCnnnnn_n'
Cuts at: 4302 5063 6404 4302
Size: 761 1341 4302
Fragments arranged by size:
4302 1341 761
BclI T'GATC_A
Cuts at: 792 1110 2961 3924 792
Size: 318 1851 963 3272
Fragments arranged by size:
3272 1851 963 318
BglII A'GATC_T
Cuts at: 522 3919 5956 522
Size: 3397 2037 970
Fragments arranged by size:
3397 2037 970
BlpI GC'TnA_GC
Cuts at: 3516 3516
Size: 6404
Bme1580I G_kGCm'C
Cuts at: 751 1982 2034 2142 2601 4029 4122 5178 751
Size: 1231 52 108 459 1428 93 1056 1977
Fragments arranged by size:
1977 1428 1231 1056 459 108 93 52
BmrI ACTGGGnnnn_n'
Cuts at: 281 3616 3697 4032 6176 281
Size: 3335 81 335 2144 509
Fragments arranged by size:
3335 2144 509 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2703 2998 4771 2703
Size: 295 1773 4336
Fragments arranged by size:
4336 1773 295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 482 563 915 158
Size: 324 81 352 5647
Fragments arranged by size:
5647 352 324 81
Bpu10I CC'TnA_GC
Cuts at: 239 470 3275 239
Size: 231 2805 3368
Fragments arranged by size:
3368 2805 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1026 3371 3580 4459 4951 5249 1026
Size: 2345 209 879 492 298 2181
Fragments arranged by size:
2345 2181 879 492 298 209
BsaI GGTCTCn'nnnn_
Cuts at: 2735 6108 2735
Size: 3373 3031
BsaAI yAC'GTr
Cuts at: 3757 3757
Size: 6404
BsaBI GATnn'nnATC
Cuts at: 6 3918 5955 6
Size: 3912 2037 455
Fragments arranged by size:
3912 2037 455
BsaHI Gr'CG_yC
Cuts at: 3835 4083 4785 6281 3835
Size: 248 702 1496 3958
Fragments arranged by size:
3958 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3593 4114 4792 5066 5213 5624 3593
Size: 521 678 274 147 411 4373
Fragments arranged by size:
4373 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3272 3302 3272
Size: 30 6374
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 155 158 1819 1883 1928 2162 2300 2348 2396
Size: 3 1661 64 45 234 138 48 48
Cuts at: 2396 2444 2492 2540 155
Size: 48 48 48 4019
Fragments arranged by size:
4019 1661 234 138 64 48 48 48
48 48 45 3
BseYI C'CCAG_C
Cuts at: 829 3829 4587 5164 5660 829
Size: 3000 758 577 496 1573
Fragments arranged by size:
3000 1573 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 701 1137 3056 701
Size: 436 1919 4049
Fragments arranged by size:
4049 1919 436
BsiEI CG_ry'CG
Cuts at: 104 3946 3992 5200 104
Size: 3842 46 1208 1308
Fragments arranged by size:
3842 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 498 568 3418 4196 4386 5178 498
Size: 70 2850 778 190 792 1724
Fragments arranged by size:
2850 1724 792 778 190 70
Bsp1286I G_dGCh'C
Cuts at: 498 568 751 1982 2034 2142 2601 3213 3418
Size: 70 183 1231 52 108 459 612 205
Cuts at: 3418 4029 4122 4196 4386 4448 5178 498
Size: 611 93 74 190 62 730 1724
Fragments arranged by size:
1724 1231 730 612 611 459 205 190
183 108 93 74 70 62 52
BspEI T'CCGG_A
Cuts at: 5624 5624
Size: 6404
BspHI T'CATG_A
Cuts at: 5580 5580
Size: 6404
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2238 2262 3454 3668 3970 4351 2238
Size: 24 1192 214 302 381 4291
Fragments arranged by size:
4291 1192 381 302 214 24
BsrBI CCG'CTC
Cuts at: 50 547 4696 4750 6234 6399 50
Size: 497 4149 54 1484 165 55
Fragments arranged by size:
4149 1484 497 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4316 4316
Size: 6404
BsrFI r'CCGG_y
Cuts at: 339 4402 4583 4792 339
Size: 4063 181 209 1951
Fragments arranged by size:
4063 1951 209 181
BssSI C'ACGA_G
Cuts at: 4675 5033 4675
Size: 358 6046
BstAPI GCAn_nnn'nTGC
Cuts at: 493 3781 493
Size: 3288 3116
BstBI TT'CG_AA
Cuts at: 4765 4765
Size: 6404
BstXI CCAn_nnnn'nTGG
Cuts at: 2274 2322 2370 2418 2466 2274
Size: 48 48 48 48 6212
Fragments arranged by size:
6212 48 48 48 48
BstYI r'GATC_y
Cuts at: 1 522 1918 2682 3429 3896 3919 4254 4500
Size: 521 1396 764 747 467 23 335 246
Cuts at: 4500 5512 5598 5610 5956 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1396 1012 764 747 521 467 449 346
335 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 933 933
Size: 6404
BtgI C'CryG_G
Cuts at: 107 1264 2006 2114 2177 2222 2315 2363 2459
Size: 1157 742 108 63 45 93 48 96
Cuts at: 2459 107
Size: 4052
Fragments arranged by size:
4052 1157 742 108 96 93 63 48
45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4354 4535 4698 4354
Size: 181 163 6060
Fragments arranged by size:
6060 181 163
BtsI GCAGTG_nn'
Cuts at: 168 719 2704 3065 3647 168
Size: 551 1985 361 582 2925
Fragments arranged by size:
2925 1985 582 551 361
ClaI AT'CG_AT
Cuts at: 786 786
Size: 6404
DraI TTT'AAA
Cuts at: 1104 2909 3403 1104
Size: 1805 494 4105
Fragments arranged by size:
4105 1805 494
DraIII CAC_nnn'GTG
Cuts at: 1204 1204
Size: 6404
DrdI GACnn_nn'nnGTC
Cuts at: 4110 4968 4110
Size: 858 5546
EaeI y'GGCC_r
Cuts at: 110 230 3214 3989 4163 4554 4581 5926 110
Size: 120 2984 775 174 391 27 1345 588
Fragments arranged by size:
2984 1345 775 588 391 174 120 27
EagI C'GGCC_G
Cuts at: 3989 3989
Size: 6404
EarI CTCTTCn'nnn_
Cuts at: 383 668 720 722 2723 2792 2801 4427 4637
Size: 285 52 2 2001 69 9 1626 210
Cuts at: 4637 383
Size: 2150
Fragments arranged by size:
2150 2001 1626 285 210 69 52 9
2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4612 4922 5068 6214 4612
Size: 310 146 1146 4802
Fragments arranged by size:
4802 1146 310 146
EcoICRI GAG'CTC
Cuts at: 566 3416 566
Size: 2850 3554
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 482 563 638 915 2773 3020 4229 4661
Size: 324 81 75 277 1858 247 1209 432
Cuts at: 4661 5408 5676 158
Size: 747 268 886
Fragments arranged by size:
1858 1209 886 747 432 324 277 268
247 81 75
EcoO109I rG'GnC_Cy
Cuts at: 747 748 1948 1978 2086 2212 3543 747
Size: 1 1200 30 108 126 1331 3608
Fragments arranged by size:
3608 1331 1200 126 108 30 1
EcoRI G'AATT_C
Cuts at: 3408 3408
Size: 6404
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2803 3273 4423 2803
Size: 470 1150 4784
Fragments arranged by size:
4784 1150 470
FspI TGC'GCA
Cuts at: 4185 4185
Size: 6404
HaeII r_GCGC'y
Cuts at: 3838 4086 5108 6087 3838
Size: 248 1022 979 4155
Fragments arranged by size:
4155 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 873 905 1705 1737 1903 1920 1935 1952 3160
Size: 32 800 32 166 17 15 17 1208
Cuts at: 3160 3192 4246 4276 4278 4308 873
Size: 32 1054 30 2 30 2969
Fragments arranged by size:
2969 1208 1054 800 166 32 32 32
30 30 17 17 15 2
HincII GTy'rAC
Cuts at: 1683 3443 1683
Size: 1760 4644
KasI G'GCGC_C
Cuts at: 3834 4082 3834
Size: 248 6156
KpnI G_GTAC'C
Cuts at: 3424 3424
Size: 6404
MfeI C'AATT_G
Cuts at: 1275 1275
Size: 6404
MluI A'CGCG_T
Cuts at: 5837 5837
Size: 6404
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5075 5259 5075
Size: 184 6220
MscI TGG'CCA
Cuts at: 112 4165 112
Size: 4053 2351
MslI CAynn'nnrTG
Cuts at: 175 877 1020 1088 1104 2272 2320 2368 2416
Size: 702 143 68 16 1168 48 48 48
Cuts at: 2416 2464 3146 4393 4520 175
Size: 48 682 1247 127 2059
Fragments arranged by size:
2059 1247 1168 702 682 143 127 68
48 48 48 48 16
MspA1I CmG'CkG
Cuts at: 290 767 3515 3829 4189 5202 5447 290
Size: 477 2748 314 360 1013 245 1247
Fragments arranged by size:
2748 1247 1013 477 360 314 245
NaeI GCC'GGC
Cuts at: 4585 4585
Size: 6404
NarI GG'CG_CC
Cuts at: 3835 4083 3835
Size: 248 6156
NcoI C'CATG_G
Cuts at: 107 2006 2177 2222 2315 2363 2459 107
Size: 1899 171 45 93 48 96 4052
Fragments arranged by size:
4052 1899 171 96 93 48 45
NdeI CA'TA_TG
Cuts at: 1073 1073
Size: 6404
NgoMIV G'CCGG_C
Cuts at: 4583 4583
Size: 6404
NspI r_CATG'y
Cuts at: 198 941 991 1246 1258 2017 2586 3177 3457
Size: 743 50 255 12 759 569 591 280
Cuts at: 3457 3957 4864 198
Size: 500 907 1738
Fragments arranged by size:
1738 907 759 743 591 569 500 280
255 50 12
PciI A'CATG_T
Cuts at: 194 937 1242 1254 3173 4860 194
Size: 743 305 12 1919 1687 1738
Fragments arranged by size:
1919 1738 1687 743 305 12
PflMI CCAn_nnn'nTGG
Cuts at: 123 123
Size: 6404
PmeI GTTT'AAAC
Cuts at: 3403 3403
Size: 6404
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5543 5575 5543
Size: 32 6372
PspOMI G'GGCC_C
Cuts at: 747 1978 747
Size: 1231 5173
PstI C_TGCA'G
Cuts at: 726 3451 4136 726
Size: 2725 685 2994
Fragments arranged by size:
2994 2725 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6404
PvuII CAG'CTG
Cuts at: 290 3829 4189 290
Size: 3539 360 2505
Fragments arranged by size:
3539 2505 360
RsrII CG'GwC_CG
Cuts at: 4599 4599
Size: 6404
SacI G_AGCT'C
Cuts at: 568 3418 568
Size: 2850 3554
SalI G'TCGA_C
Cuts at: 3441 3441
Size: 6404
SapI GCTCTTCn'nnn_
Cuts at: 720 4427 4637 720
Size: 3707 210 2487
Fragments arranged by size:
3707 2487 210
SbfI CC_TGCA'GG
Cuts at: 3451 3451
Size: 6404
ScaI AGT'ACT
Cuts at: 5921 5921
Size: 6404
SexAI A'CCwGG_T
Cuts at: 302 2826 302
Size: 2524 3880
SfcI C'TryA_G
Cuts at: 33 722 1318 3447 4132 5125 5316 33
Size: 689 596 2129 685 993 191 1121
Fragments arranged by size:
2129 1121 993 689 685 596 191
SfoI GGC'GCC
Cuts at: 3836 4084 3836
Size: 248 6156
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6404
SmaI CCC'GGG
Cuts at: 3426 3426
Size: 6404
SmlI C'TyrA_G
Cuts at: 1041 3386 3559 4474 4966 5228 1041
Size: 2345 173 915 492 262 2217
Fragments arranged by size:
2345 2217 915 492 262 173
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6404
SphI G_CATG'C
Cuts at: 2017 3457 3957 2017
Size: 1440 500 4464
Fragments arranged by size:
4464 1440 500
SspI AAT'ATT
Cuts at: 1428 1428
Size: 6404
StyI C'CwwG_G
Cuts at: 107 743 922 2006 2177 2222 2315 2363 2459
Size: 636 179 1084 171 45 93 48 96
Cuts at: 2459 3257 3538 3892 107
Size: 798 281 354 2619
Fragments arranged by size:
2619 1084 798 636 354 281 179 171
96 93 48 45
SwaI ATTT'AAAT
Cuts at: 1104 1104
Size: 6404
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 130 2125 3987 4120 4579 4788 130
Size: 1995 1862 133 459 209 1746
Fragments arranged by size:
1995 1862 1746 459 209 133
TatI w'GTAC_w
Cuts at: 1840 2148 3365 5919 1840
Size: 308 1217 2554 2325
Fragments arranged by size:
2554 2325 1217 308
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5921 5953 5921
Size: 32 6372
Tth111I GACn'n_nGTC
Cuts at: 4201 4201
Size: 6404
XbaI T'CTAG_A
Cuts at: 3435 3435
Size: 6404
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 120 225 826 1696 3072 3899 120
Size: 105 601 870 1376 827 2625
Fragments arranged by size:
2625 1376 870 827 601 105
XmaI C'CCGG_G
Cuts at: 3424 3424
Size: 6404
XmnI GAAnn'nnTTC
Cuts at: 1632 1632
Size: 6404
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PflMI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI AvrII BfrBI
BglI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII
BstEII Bsu36I CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI StuI XhoI ZraI