Restriction map for
pF1KB5120
(Circular) (Six-base) MAPSORT of: pF1KB5120.seq Check: 8747 from: 1 to: 4373
pF1KB5120 4373 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1185 1185
Size: 4373
AccI GT'mk_AC
Cuts at: 524 1075 1411 524
Size: 551 336 3486
Fragments arranged by size:
3486 551 336
Acc65I G'GTAC_C
Cuts at: 1389 1389
Size: 4373
AclI AA'CG_TT
Cuts at: 4213 4213
Size: 4373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 2198 2630 2983 3645 152
Size: 2046 432 353 662 880
Fragments arranged by size:
2046 880 662 432 353
AflIII A'CryG_T
Cuts at: 1725 3525 3806 1725
Size: 1800 281 2292
Fragments arranged by size:
2292 1800 281
AleI CACnn'nnGTG
Cuts at: 286 286
Size: 4373
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 172 172
Size: 4373
AlwNI CAG_nnn'CTG
Cuts at: 1678 3116 1678
Size: 1438 2935
ApaLI G'TGCA_C
Cuts at: 1112 3211 1112
Size: 2099 2274
ApoI r'AATT_y
Cuts at: 976 982 1377 3726 3982 976
Size: 6 395 2349 256 1367
Fragments arranged by size:
2349 1367 395 256 6
AseI AT'TA_AT
Cuts at: 20 20
Size: 4373
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4373
AvaI C'yCGr_G
Cuts at: 1393 1393
Size: 4373
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 674 707 674
Size: 33 4340
BamHI G'GATC_C
Cuts at: 675 1398 675
Size: 723 3650
BanI G'GyrC_C
Cuts at: 777 1389 1803 2051 2086 777
Size: 612 414 248 35 3064
Fragments arranged by size:
3064 612 414 248 35
BanII G_rGCy'C
Cuts at: 401 1387 2417 401
Size: 986 1030 2357
Fragments arranged by size:
2357 1030 986
BbeI G_GCGC'C
Cuts at: 1807 2055 1807
Size: 248 4125
BbsI GAAGACnn'nnnn_
Cuts at: 1011 1011
Size: 4373
BciVI GTATCCnnnnn_n'
Cuts at: 2271 3327 4373 2271
Size: 1056 1046 2271
Fragments arranged by size:
2271 1056 1046
BclI T'GATC_A
Cuts at: 1893 1893
Size: 4373
BglII A'GATC_T
Cuts at: 1888 3925 1888
Size: 2037 2336
BlpI GC'TnA_GC
Cuts at: 851 1485 851
Size: 634 3739
Bme1580I G_kGCm'C
Cuts at: 780 1116 1998 2091 3215 780
Size: 336 882 93 1124 1938
Fragments arranged by size:
1938 1124 882 336 93
BmrI ACTGGGnnnn_n'
Cuts at: 1585 1666 2001 4145 1585
Size: 81 335 2144 1813
Fragments arranged by size:
2144 1813 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2740 2740
Size: 4373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 444 1090 1334 385
Size: 59 646 244 3424
Fragments arranged by size:
3424 646 244 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1184 1549 2428 3142 3440 1184
Size: 365 879 714 298 2117
Fragments arranged by size:
2117 879 714 365 298
BsaI GGTCTCn'nnnn_
Cuts at: 1310 4077 1310
Size: 2767 1606
BsaAI yAC'GTr
Cuts at: 1726 1726
Size: 4373
BsaBI GATnn'nnATC
Cuts at: 6 1887 3924 6
Size: 1881 2037 455
Fragments arranged by size:
2037 1881 455
BsaHI Gr'CG_yC
Cuts at: 1804 2052 2754 4250 1804
Size: 248 702 1496 1927
Fragments arranged by size:
1927 1496 702 248
BsaWI w'CCGG_w
Cuts at: 513 1038 1562 2083 2761 3172 3319 3593 513
Size: 525 524 521 678 411 147 274 1293
Fragments arranged by size:
1293 678 525 524 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 335 365 548 578 335
Size: 30 183 30 4130
Fragments arranged by size:
4130 183 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 749 1235 749
Size: 486 3887
BseYI C'CCAG_C
Cuts at: 1798 2556 3221 3629 1798
Size: 758 665 408 2542
Fragments arranged by size:
2542 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 1163 178
Size: 985 3388
BsiEI CG_ry'CG
Cuts at: 104 1915 1961 3191 104
Size: 1811 46 1230 1286
Fragments arranged by size:
1811 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 401 1116 1387 2165 2355 3215 401
Size: 715 271 778 190 860 1559
Fragments arranged by size:
1559 860 778 715 271 190
Bsp1286I G_dGCh'C
Cuts at: 401 780 1116 1387 1998 2091 2165 2355 2417
Size: 379 336 271 611 93 74 190 62
Cuts at: 2417 3215 401
Size: 798 1559
Fragments arranged by size:
1559 798 611 379 336 271 190 93
74 62
BspEI T'CCGG_A
Cuts at: 513 513
Size: 4373
BspHI T'CATG_A
Cuts at: 407 2805 407
Size: 2398 1975
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1185 1423 1637 1939 2320 1185
Size: 238 214 302 381 3238
Fragments arranged by size:
3238 381 302 238 214
BsrBI CCG'CTC
Cuts at: 50 2665 2719 4203 4368 50
Size: 2615 54 1484 165 55
Fragments arranged by size:
2615 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2285 2285
Size: 4373
BsrFI r'CCGG_y
Cuts at: 153 2371 2552 153
Size: 2218 181 1974
Fragments arranged by size:
2218 1974 181
BssSI C'ACGA_G
Cuts at: 2644 3352 2644
Size: 708 3665
BstAPI GCAn_nnn'nTGC
Cuts at: 1750 1750
Size: 4373
BstBI TT'CG_AA
Cuts at: 2734 2734
Size: 4373
BstXI CCAn_nnnn'nTGG
Cuts at: 631 631
Size: 4373
BstYI r'GATC_y
Cuts at: 1 675 1398 1865 1888 2223 2469 2775 2787
Size: 674 723 467 23 335 246 306 12
Cuts at: 2787 2873 3925 1
Size: 86 1052 449
Fragments arranged by size:
1052 723 674 467 449 335 306 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 525 1076 525
Size: 551 3822
BtgI C'CryG_G
Cuts at: 1320 1320
Size: 4373
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2323 2504 2667 2323
Size: 181 163 4029
Fragments arranged by size:
4029 181 163
BtsI GCAGTG_nn'
Cuts at: 1616 1616
Size: 4373
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 117 152 209 244 117
Size: 35 57 35 4246
Fragments arranged by size:
4246 57 35 35
DraI TTT'AAA
Cuts at: 981 1372 981
Size: 391 3982
DrdI GACnn_nn'nnGTC
Cuts at: 545 1147 2079 3423 545
Size: 602 932 1344 1495
Fragments arranged by size:
1495 1344 932 602
EaeI y'GGCC_r
Cuts at: 1958 2132 2523 2550 3895 1958
Size: 174 391 27 1345 2436
Fragments arranged by size:
2436 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1958 1958
Size: 4373
EarI CTCTTCn'nnn_
Cuts at: 1189 2396 2606 1189
Size: 1207 210 2956
Fragments arranged by size:
2956 1207 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2581 3323 3469 4183 2581
Size: 742 146 714 2771
Fragments arranged by size:
2771 742 714 146
EcoICRI GAG'CTC
Cuts at: 399 1385 399
Size: 986 3387
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 385 444 1090 1334 2198 2630 2983 3645
Size: 233 59 646 244 864 432 353 662
Cuts at: 3645 152
Size: 880
Fragments arranged by size:
880 864 662 646 432 353 244 233
59
EcoO109I rG'GnC_Cy
Cuts at: 921 1140 1512 921
Size: 219 372 3782
Fragments arranged by size:
3782 372 219
EcoRI G'AATT_C
Cuts at: 1377 1377
Size: 4373
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 346 2392 346
Size: 2046 2327
FspI TGC'GCA
Cuts at: 2154 2154
Size: 4373
HaeII r_GCGC'y
Cuts at: 1807 2055 3285 4056 1807
Size: 248 1230 771 2124
Fragments arranged by size:
2124 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 335 367 796 814 828 846 2215 2245 2247
Size: 32 429 18 14 18 1369 30 2
Cuts at: 2247 2277 335
Size: 30 2431
Fragments arranged by size:
2431 1369 429 32 30 30 18 18
14 2
HincII GTy'rAC
Cuts at: 1412 1412
Size: 4373
HindIII A'AGCT_T
Cuts at: 1106 1106
Size: 4373
KasI G'GCGC_C
Cuts at: 1803 2051 1803
Size: 248 4125
KpnI G_GTAC'C
Cuts at: 1393 1393
Size: 4373
MfeI C'AATT_G
Cuts at: 1046 1046
Size: 4373
MluI A'CGCG_T
Cuts at: 3806 3806
Size: 4373
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 653 958 1047 1382 3132 3316 653
Size: 305 89 335 1750 184 1710
Fragments arranged by size:
1750 1710 335 305 184 89
MscI TGG'CCA
Cuts at: 2134 2134
Size: 4373
MslI CAynn'nnrTG
Cuts at: 286 2362 2489 286
Size: 2076 127 2170
Fragments arranged by size:
2170 2076 127
MspA1I CmG'CkG
Cuts at: 600 726 1267 1484 1798 2158 2942 3187 600
Size: 126 541 217 314 360 784 245 1786
Fragments arranged by size:
1786 784 541 360 314 245 217 126
NaeI GCC'GGC
Cuts at: 2554 2554
Size: 4373
NarI GG'CG_CC
Cuts at: 1804 2052 1804
Size: 248 4125
NgoMIV G'CCGG_C
Cuts at: 2552 2552
Size: 4373
NspI r_CATG'y
Cuts at: 1426 1926 3529 1426
Size: 500 1603 2270
Fragments arranged by size:
2270 1603 500
PciI A'CATG_T
Cuts at: 3525 3525
Size: 4373
PmeI GTTT'AAAC
Cuts at: 1372 1372
Size: 4373
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2819 2851 2819
Size: 32 4341
PpuMI rG'GwC_Cy
Cuts at: 921 1140 921
Size: 219 4154
PshAI GACnn'nnGTC
Cuts at: 1015 1015
Size: 4373
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 586 618 952 984 586
Size: 32 334 32 3975
Fragments arranged by size:
3975 334 32 32
PstI C_TGCA'G
Cuts at: 1420 2105 1420
Size: 685 3688
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4373
PvuII CAG'CTG
Cuts at: 600 726 1267 1798 2158 600
Size: 126 541 531 360 2815
Fragments arranged by size:
2815 541 531 360 126
RsrII CG'GwC_CG
Cuts at: 2568 2568
Size: 4373
SacI G_AGCT'C
Cuts at: 401 1387 401
Size: 986 3387
SalI G'TCGA_C
Cuts at: 1410 1410
Size: 4373
SanDI GG'GwC_CC
Cuts at: 1140 1140
Size: 4373
SapI GCTCTTCn'nnn_
Cuts at: 2396 2606 2396
Size: 210 4163
SbfI CC_TGCA'GG
Cuts at: 1420 1420
Size: 4373
ScaI AGT'ACT
Cuts at: 3890 3890
Size: 4373
SfcI C'TryA_G
Cuts at: 33 543 771 1416 2101 3069 3260 33
Size: 510 228 645 685 968 191 1146
Fragments arranged by size:
1146 968 685 645 510 228 191
SfoI GGC'GCC
Cuts at: 1805 2053 1805
Size: 248 4125
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4373
SmaI CCC'GGG
Cuts at: 1395 1395
Size: 4373
SmlI C'TyrA_G
Cuts at: 1199 1528 2443 3157 3419 1199
Size: 329 915 714 262 2153
Fragments arranged by size:
2153 915 714 329 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4373
SphI G_CATG'C
Cuts at: 1426 1926 1426
Size: 500 3873
SspI AAT'ATT
Cuts at: 875 875
Size: 4373
StyI C'CwwG_G
Cuts at: 1006 1507 1861 1006
Size: 501 354 3518
Fragments arranged by size:
3518 501 354
SwaI ATTT'AAAT
Cuts at: 981 981
Size: 4373
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1956 2089 2548 2757 1956
Size: 133 459 209 3572
Fragments arranged by size:
3572 459 209 133
TatI w'GTAC_w
Cuts at: 3888 3888
Size: 4373
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3890 3922 3890
Size: 32 4341
Tth111I GACn'n_nGTC
Cuts at: 2170 2170
Size: 4373
XbaI T'CTAG_A
Cuts at: 1404 1404
Size: 4373
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1868 1868
Size: 4373
XmaI C'CCGG_G
Cuts at: 1393 1393
Size: 4373
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PpuMI PshAI PsrI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI ApaI AscI
AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII Bsu36I ClaI
DraIII EcoNI EcoRV FseI FspAI HpaI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PsiI PspOMI PspXI SacII SexAI SfiI SgrAI
SnaBI SrfI StuI XhoI XmnI ZraI