Restriction map for
pF1KB5121
(Circular) (Six-base) MAPSORT of: pF1KB5121.seq Check: 2826 from: 1 to: 4535
pF1KB5121 4535 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 290 1190 1573 290
Size: 900 383 3252
Fragments arranged by size:
3252 900 383
Acc65I G'GTAC_C
Cuts at: 459 1374 1551 459
Size: 915 177 3443
Fragments arranged by size:
3443 915 177
AclI AA'CG_TT
Cuts at: 4375 4375
Size: 4535
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2360 2792 3145 3807 2360
Size: 432 353 662 3088
Fragments arranged by size:
3088 662 432 353
AflIII A'CryG_T
Cuts at: 206 1527 1887 3687 3968 206
Size: 1321 360 1800 281 773
Fragments arranged by size:
1800 1321 773 360 281
AhdI GACnn_n'nnGTC
Cuts at: 1461 1461
Size: 4535
AleI CACnn'nnGTG
Cuts at: 952 1264 952
Size: 312 4223
AlwNI CAG_nnn'CTG
Cuts at: 709 1840 3278 709
Size: 1131 1438 1966
Fragments arranged by size:
1966 1438 1131
ApaI G_GGCC'C
Cuts at: 284 284
Size: 4535
ApaLI G'TGCA_C
Cuts at: 179 3373 179
Size: 3194 1341
ApoI r'AATT_y
Cuts at: 676 1446 1539 3888 4144 676
Size: 770 93 2349 256 1067
Fragments arranged by size:
2349 1067 770 256 93
AscI GG'CGCG_CC
Cuts at: 1353 1353
Size: 4535
AseI AT'TA_AT
Cuts at: 20 20
Size: 4535
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4535
AvaI C'yCGr_G
Cuts at: 1555 1555
Size: 4535
BamHI G'GATC_C
Cuts at: 1560 1560
Size: 4535
BanI G'GyrC_C
Cuts at: 459 1374 1551 1965 2213 2248 459
Size: 915 177 414 248 35 2746
Fragments arranged by size:
2746 915 414 248 177 35
BanII G_rGCy'C
Cuts at: 284 1549 2579 284
Size: 1265 1030 2240
Fragments arranged by size:
2240 1265 1030
BbeI G_GCGC'C
Cuts at: 1969 2217 1969
Size: 248 4287
BbvCI CC'TCA_GC
Cuts at: 1213 1213
Size: 4535
BciVI GTATCCnnnnn_n'
Cuts at: 806 2433 3489 4535 806
Size: 1627 1056 1046 806
Fragments arranged by size:
1627 1056 1046 806
BclI T'GATC_A
Cuts at: 2055 2055
Size: 4535
BfrBI ATG'CAT
Cuts at: 919 919
Size: 4535
BglII A'GATC_T
Cuts at: 2050 4087 2050
Size: 2037 2498
BlpI GC'TnA_GC
Cuts at: 1647 1647
Size: 4535
Bme1580I G_kGCm'C
Cuts at: 183 284 2160 2253 3377 183
Size: 101 1876 93 1124 1341
Fragments arranged by size:
1876 1341 1124 101 93
BmrI ACTGGGnnnn_n'
Cuts at: 1747 1828 2163 4307 1747
Size: 81 335 2144 1975
Fragments arranged by size:
2144 1975 335 81
BmtI G_CTAG'C
Cuts at: 1133 1133
Size: 4535
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2902 2902
Size: 4535
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 658 1093 1189 658
Size: 435 96 4004
Fragments arranged by size:
4004 435 96
Bpu10I CC'TnA_GC
Cuts at: 1213 1330 1213
Size: 117 4418
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1711 2590 3304 3602 1711
Size: 879 714 298 2644
Fragments arranged by size:
2644 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 336 985 4239 336
Size: 649 3254 632
Fragments arranged by size:
3254 649 632
BsaAI yAC'GTr
Cuts at: 216 1888 216
Size: 1672 2863
BsaBI GATnn'nnATC
Cuts at: 6 304 1031 2049 4086 6
Size: 298 727 1018 2037 455
Fragments arranged by size:
2037 1018 727 455 298
BsaHI Gr'CG_yC
Cuts at: 1193 1966 2214 2916 4412 1193
Size: 773 248 702 1496 1316
Fragments arranged by size:
1496 1316 773 702 248
BsaWI w'CCGG_w
Cuts at: 1229 1724 2245 2923 3334 3481 3755 1229
Size: 495 521 678 411 147 274 2009
Fragments arranged by size:
2009 678 521 495 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 316 346 316
Size: 30 4505
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 707 1201 707
Size: 494 4041
BseYI C'CCAG_C
Cuts at: 199 1378 1409 1960 2718 3383 3791 199
Size: 1179 31 551 758 665 408 943
Fragments arranged by size:
1179 943 758 665 551 408 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 615 615
Size: 4535
BsiEI CG_ry'CG
Cuts at: 104 268 2077 2123 3353 104
Size: 164 1809 46 1230 1286
Fragments arranged by size:
1809 1286 1230 164 46
BsiHKAI G_wGCw'C
Cuts at: 183 1259 1549 2327 2517 3377 183
Size: 1076 290 778 190 860 1341
Fragments arranged by size:
1341 1076 860 778 290 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1231 1231
Size: 4535
Bsp1286I G_dGCh'C
Cuts at: 183 284 1259 1549 2160 2253 2327 2517 2579
Size: 101 975 290 611 93 74 190 62
Cuts at: 2579 3377 183
Size: 798 1341
Fragments arranged by size:
1341 975 798 611 290 190 101 93
74 62
BspEI T'CCGG_A
Cuts at: 1229 1229
Size: 4535
BspHI T'CATG_A
Cuts at: 2967 2967
Size: 4535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1585 1799 2101 2482 1585
Size: 214 302 381 3638
Fragments arranged by size:
3638 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2827 2881 4365 4530 50
Size: 2777 54 1484 165 55
Fragments arranged by size:
2777 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2447 2447
Size: 4535
BsrFI r'CCGG_y
Cuts at: 1364 2533 2714 1364
Size: 1169 181 3185
Fragments arranged by size:
3185 1169 181
BsrGI T'GTAC_A
Cuts at: 1524 1524
Size: 4535
BssHII G'CGCG_C
Cuts at: 1353 1353
Size: 4535
BssSI C'ACGA_G
Cuts at: 1260 2806 3514 1260
Size: 1546 708 2281
Fragments arranged by size:
2281 1546 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1912 1912
Size: 4535
BstBI TT'CG_AA
Cuts at: 603 2896 603
Size: 2293 2242
BstYI r'GATC_y
Cuts at: 1 597 1004 1424 1560 2027 2050 2385 2631
Size: 596 407 420 136 467 23 335 246
Cuts at: 2631 2937 2949 3035 4087 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 596 467 449 420 407 335 306
246 136 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 985 985
Size: 4535
BtgI C'CryG_G
Cuts at: 167 1197 167
Size: 1030 3505
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2485 2666 2829 2485
Size: 181 163 4191
Fragments arranged by size:
4191 181 163
BtsI GCAGTG_nn'
Cuts at: 1778 1778
Size: 4535
ClaI AT'CG_AT
Cuts at: 308 308
Size: 4535
DraI TTT'AAA
Cuts at: 1534 1534
Size: 4535
DrdI GACnn_nn'nnGTC
Cuts at: 2241 3585 2241
Size: 1344 3191
EaeI y'GGCC_r
Cuts at: 265 2120 2294 2685 2712 4057 265
Size: 1855 174 391 27 1345 743
Fragments arranged by size:
1855 1345 743 391 174 27
EagI C'GGCC_G
Cuts at: 265 2120 265
Size: 1855 2680
EarI CTCTTCn'nnn_
Cuts at: 2558 2768 2558
Size: 210 4325
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2743 3485 3631 4345 2743
Size: 742 146 714 2933
Fragments arranged by size:
2933 742 714 146
EcoICRI GAG'CTC
Cuts at: 1547 1547
Size: 4535
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 658 1093 1189 2360 2792 3145 3807 658
Size: 435 96 1171 432 353 662 1386
Fragments arranged by size:
1386 1171 662 435 432 353 96
EcoO109I rG'GnC_Cy
Cuts at: 280 281 1111 1674 280
Size: 1 830 563 3141
Fragments arranged by size:
3141 830 563 1
EcoRI G'AATT_C
Cuts at: 1446 1539 1446
Size: 93 4442
EcoRV GAT'ATC
Cuts at: 610 610
Size: 4535
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 416 2554 416
Size: 2138 2397
FspI TGC'GCA
Cuts at: 2316 2316
Size: 4535
HaeII r_GCGC'y
Cuts at: 1307 1969 2217 3447 4218 1307
Size: 662 248 1230 771 1624
Fragments arranged by size:
1624 1230 771 662 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 316 348 475 507 554 586 1447 1479 2377
Size: 32 127 32 47 32 861 32 898
Cuts at: 2377 2407 2409 2439 316
Size: 30 2 30 2412
Fragments arranged by size:
2412 898 861 127 47 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 291 750 1191 1574 291
Size: 459 441 383 3252
Fragments arranged by size:
3252 459 441 383
KasI G'GCGC_C
Cuts at: 1965 2213 1965
Size: 248 4287
KpnI G_GTAC'C
Cuts at: 463 1378 1555 463
Size: 915 177 3443
Fragments arranged by size:
3443 915 177
MfeI C'AATT_G
Cuts at: 743 743
Size: 4535
MluI A'CGCG_T
Cuts at: 3968 3968
Size: 4535
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3294 3478 3294
Size: 184 4351
MscI TGG'CCA
Cuts at: 2296 2296
Size: 4535
MslI CAynn'nnrTG
Cuts at: 325 542 952 1264 2524 2651 325
Size: 217 410 312 1260 127 2209
Fragments arranged by size:
2209 1260 410 312 217 127
MspA1I CmG'CkG
Cuts at: 583 1646 1960 2320 3104 3349 583
Size: 1063 314 360 784 245 1769
Fragments arranged by size:
1769 1063 784 360 314 245
NaeI GCC'GGC
Cuts at: 2716 2716
Size: 4535
NarI GG'CG_CC
Cuts at: 1966 2214 1966
Size: 248 4287
NcoI C'CATG_G
Cuts at: 167 167
Size: 4535
NgoMIV G'CCGG_C
Cuts at: 2714 2714
Size: 4535
NheI G'CTAG_C
Cuts at: 1129 1129
Size: 4535
NsiI A_TGCA'T
Cuts at: 921 921
Size: 4535
NspI r_CATG'y
Cuts at: 210 590 864 1588 2088 3691 210
Size: 380 274 724 500 1603 1054
Fragments arranged by size:
1603 1054 724 500 380 274
PciI A'CATG_T
Cuts at: 206 3687 206
Size: 3481 1054
PmeI GTTT'AAAC
Cuts at: 1534 1534
Size: 4535
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2981 3013 2981
Size: 32 4503
PspOMI G'GGCC_C
Cuts at: 280 280
Size: 4535
PstI C_TGCA'G
Cuts at: 927 1582 2267 927
Size: 655 685 3195
Fragments arranged by size:
3195 685 655
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4535
PvuII CAG'CTG
Cuts at: 583 1960 2320 583
Size: 1377 360 2798
Fragments arranged by size:
2798 1377 360
RsrII CG'GwC_CG
Cuts at: 2730 2730
Size: 4535
SacI G_AGCT'C
Cuts at: 1549 1549
Size: 4535
SalI G'TCGA_C
Cuts at: 289 1189 1572 289
Size: 900 383 3252
Fragments arranged by size:
3252 900 383
SapI GCTCTTCn'nnn_
Cuts at: 2558 2768 2558
Size: 210 4325
SbfI CC_TGCA'GG
Cuts at: 1582 1582
Size: 4535
ScaI AGT'ACT
Cuts at: 4052 4052
Size: 4535
SfcI C'TryA_G
Cuts at: 33 923 1578 2263 3231 3422 33
Size: 890 655 685 968 191 1146
Fragments arranged by size:
1146 968 890 685 655 191
SfoI GGC'GCC
Cuts at: 1967 2215 1967
Size: 248 4287
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4535
SmaI CCC'GGG
Cuts at: 1557 1557
Size: 4535
SmlI C'TyrA_G
Cuts at: 1690 2605 3319 3581 1690
Size: 915 714 262 2644
Fragments arranged by size:
2644 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4535
SphI G_CATG'C
Cuts at: 1588 2088 1588
Size: 500 4035
StuI AGG'CCT
Cuts at: 1345 1345
Size: 4535
StyI C'CwwG_G
Cuts at: 167 1277 1669 2023 167
Size: 1110 392 354 2679
Fragments arranged by size:
2679 1110 392 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 882 994 2118 2251 2710 2919 882
Size: 112 1124 133 459 209 2498
Fragments arranged by size:
2498 1124 459 209 133 112
TatI w'GTAC_w
Cuts at: 117 1289 1524 4050 117
Size: 1172 235 2526 602
Fragments arranged by size:
2526 1172 602 235
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4052 4084 4052
Size: 32 4503
Tth111I GACn'n_nGTC
Cuts at: 328 2332 328
Size: 2004 2531
XbaI T'CTAG_A
Cuts at: 1566 1566
Size: 4535
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 324 2030 324
Size: 1706 2829
XmaI C'CCGG_G
Cuts at: 1555 1555
Size: 4535
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AlwNI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NsiI NspI PciI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AlfI AloI AvrII
BaeI BbsI BcgI BglI BmgBI BsiWI BsmI BstEII
BstXI BstZ17I CspCI DraIII EcoNI FseI FspAI HindIII
HpaI NdeI NotI NruI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI