Restriction map for
pF1KB5122
(Circular) (Six-base) MAPSORT of: pF1KB5122.seq Check: 1608 from: 1 to: 4655
pF1KB5122 4655 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 276 333 750 965 1394 1693 276
Size: 57 417 215 429 299 3238
Fragments arranged by size:
3238 429 417 299 215 57
Acc65I G'GTAC_C
Cuts at: 316 1671 316
Size: 1355 3300
AclI AA'CG_TT
Cuts at: 4495 4495
Size: 4655
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 638 2480 2912 3265 3927 638
Size: 1842 432 353 662 1366
Fragments arranged by size:
1842 1366 662 432 353
AflIII A'CryG_T
Cuts at: 2007 3807 4088 2007
Size: 1800 281 2574
Fragments arranged by size:
2574 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1544 1544
Size: 4655
AlwNI CAG_nnn'CTG
Cuts at: 436 439 670 1960 3398 436
Size: 3 231 1290 1438 1693
Fragments arranged by size:
1693 1438 1290 231 3
ApaLI G'TGCA_C
Cuts at: 3493 3493
Size: 4655
ApoI r'AATT_y
Cuts at: 942 1286 1659 4008 4264 942
Size: 344 373 2349 256 1333
Fragments arranged by size:
2349 1333 373 344 256
AseI AT'TA_AT
Cuts at: 20 305 20
Size: 285 4370
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4655
AvaI C'yCGr_G
Cuts at: 1675 1675
Size: 4655
BamHI G'GATC_C
Cuts at: 1680 1680
Size: 4655
BanI G'GyrC_C
Cuts at: 229 316 1671 2085 2333 2368 229
Size: 87 1355 414 248 35 2516
Fragments arranged by size:
2516 1355 414 248 87 35
BanII G_rGCy'C
Cuts at: 1669 2699 1669
Size: 1030 3625
BbeI G_GCGC'C
Cuts at: 2089 2337 2089
Size: 248 4407
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1388 1422 1388
Size: 34 4621
BciVI GTATCCnnnnn_n'
Cuts at: 2553 3609 4655 2553
Size: 1056 1046 2553
Fragments arranged by size:
2553 1056 1046
BclI T'GATC_A
Cuts at: 2175 2175
Size: 4655
BfrBI ATG'CAT
Cuts at: 1409 1409
Size: 4655
BglII A'GATC_T
Cuts at: 2170 4207 2170
Size: 2037 2618
BlpI GC'TnA_GC
Cuts at: 1767 1767
Size: 4655
Bme1580I G_kGCm'C
Cuts at: 234 2280 2373 3497 234
Size: 2046 93 1124 1392
Fragments arranged by size:
2046 1392 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1867 1948 2283 4427 1867
Size: 81 335 2144 2095
Fragments arranged by size:
2144 2095 335 81
BmtI G_CTAG'C
Cuts at: 887 887
Size: 4655
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3022 3022
Size: 4655
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 692 980 692
Size: 288 4367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1831 2710 3424 3722 1831
Size: 879 714 298 2764
Fragments arranged by size:
2764 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4359 4359
Size: 4655
BsaAI yAC'GTr
Cuts at: 2008 2008
Size: 4655
BsaBI GATnn'nnATC
Cuts at: 6 259 1408 2169 4206 6
Size: 253 1149 761 2037 455
Fragments arranged by size:
2037 1149 761 455 253
BsaHI Gr'CG_yC
Cuts at: 2086 2334 3036 4532 2086
Size: 248 702 1496 2209
Fragments arranged by size:
2209 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1844 2365 3043 3454 3601 3875 1844
Size: 521 678 411 147 274 2624
Fragments arranged by size:
2624 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1583 1613 1583
Size: 30 4625
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 523 523
Size: 4655
BseYI C'CCAG_C
Cuts at: 2080 2838 3503 3911 2080
Size: 758 665 408 2824
Fragments arranged by size:
2824 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2197 2243 3473 104
Size: 2093 46 1230 1286
Fragments arranged by size:
2093 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 522 1533 1669 2447 2637 3497 522
Size: 1011 136 778 190 860 1680
Fragments arranged by size:
1680 1011 860 778 190 136
BsmI GAATG_Cn'
Cuts at: 1097 1587 1097
Size: 490 4165
Bsp1286I G_dGCh'C
Cuts at: 234 522 1533 1669 2280 2373 2447 2637 2699
Size: 288 1011 136 611 93 74 190 62
Cuts at: 2699 3497 234
Size: 798 1392
Fragments arranged by size:
1392 1011 798 611 288 190 136 93
74 62
BspHI T'CATG_A
Cuts at: 3087 3087
Size: 4655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1460 1705 1919 2221 2602 1460
Size: 245 214 302 381 3513
Fragments arranged by size:
3513 381 302 245 214
BsrBI CCG'CTC
Cuts at: 50 2947 3001 4485 4650 50
Size: 2897 54 1484 165 55
Fragments arranged by size:
2897 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2567 2567
Size: 4655
BsrFI r'CCGG_y
Cuts at: 2653 2834 2653
Size: 181 4474
BsrGI T'GTAC_A
Cuts at: 1442 1503 1442
Size: 61 4594
BssSI C'ACGA_G
Cuts at: 2926 3634 2926
Size: 708 3947
BstAPI GCAn_nnn'nTGC
Cuts at: 436 439 2032 436
Size: 3 1593 3059
Fragments arranged by size:
3059 1593 3
BstBI TT'CG_AA
Cuts at: 3016 3016
Size: 4655
BstEII G'GTnAC_C
Cuts at: 874 874
Size: 4655
BstYI r'GATC_y
Cuts at: 1 472 1680 2147 2170 2505 2751 3057 3069
Size: 471 1208 467 23 335 246 306 12
Cuts at: 3069 3155 4207 1
Size: 86 1052 449
Fragments arranged by size:
1208 1052 471 467 449 335 306 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 751 751
Size: 4655
Bsu36I CC'TnA_GG
Cuts at: 891 891
Size: 4655
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2605 2786 2949 2605
Size: 181 163 4311
Fragments arranged by size:
4311 181 163
BtsI GCAGTG_nn'
Cuts at: 126 1898 126
Size: 1772 2883
DraI TTT'AAA
Cuts at: 1226 1654 1226
Size: 428 4227
DrdI GACnn_nn'nnGTC
Cuts at: 1255 2361 3705 1255
Size: 1106 1344 2205
Fragments arranged by size:
2205 1344 1106
EaeI y'GGCC_r
Cuts at: 2240 2414 2805 2832 4177 2240
Size: 174 391 27 1345 2718
Fragments arranged by size:
2718 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2240 2240
Size: 4655
EarI CTCTTCn'nnn_
Cuts at: 2678 2888 2678
Size: 210 4445
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2863 3605 3751 4465 2863
Size: 742 146 714 3053
Fragments arranged by size:
3053 742 714 146
EcoICRI GAG'CTC
Cuts at: 1667 1667
Size: 4655
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 638 692 980 2480 2912 3265 3927 638
Size: 54 288 1500 432 353 662 1366
Fragments arranged by size:
1500 1366 662 432 353 288 54
EcoO109I rG'GnC_Cy
Cuts at: 907 1179 1794 907
Size: 272 615 3768
Fragments arranged by size:
3768 615 272
EcoRI G'AATT_C
Cuts at: 1659 1659
Size: 4655
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 838 1505 2674 838
Size: 667 1169 2819
Fragments arranged by size:
2819 1169 667
FspI TGC'GCA
Cuts at: 2436 2436
Size: 4655
HaeII r_GCGC'y
Cuts at: 2089 2337 3567 4338 2089
Size: 248 1230 771 2406
Fragments arranged by size:
2406 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 334 366 778 810 1314 1346 1515 1547 2497
Size: 32 412 32 504 32 169 32 950
Cuts at: 2497 2527 2529 2559 334
Size: 30 2 30 2430
Fragments arranged by size:
2430 950 504 412 169 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 132 334 1694 132
Size: 202 1360 3093
Fragments arranged by size:
3093 1360 202
HindIII A'AGCT_T
Cuts at: 398 412 398
Size: 14 4641
KasI G'GCGC_C
Cuts at: 2085 2333 2085
Size: 248 4407
KpnI G_GTAC'C
Cuts at: 320 1675 320
Size: 1355 3300
MfeI C'AATT_G
Cuts at: 1251 1251
Size: 4655
MluI A'CGCG_T
Cuts at: 4088 4088
Size: 4655
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1333 1581 3414 3598 1333
Size: 248 1833 184 2390
Fragments arranged by size:
2390 1833 248 184
MscI TGG'CCA
Cuts at: 2416 2416
Size: 4655
MslI CAynn'nnrTG
Cuts at: 924 998 1154 2644 2771 924
Size: 74 156 1490 127 2808
Fragments arranged by size:
2808 1490 156 127 74
MspA1I CmG'CkG
Cuts at: 247 436 445 454 1766 2080 2440 3224 3469
Size: 189 9 9 1312 314 360 784 245
Cuts at: 3469 247
Size: 1433
Fragments arranged by size:
1433 1312 784 360 314 245 189 9
9
NaeI GCC'GGC
Cuts at: 2836 2836
Size: 4655
NarI GG'CG_CC
Cuts at: 2086 2334 2086
Size: 248 4407
NgoMIV G'CCGG_C
Cuts at: 2834 2834
Size: 4655
NheI G'CTAG_C
Cuts at: 883 883
Size: 4655
NsiI A_TGCA'T
Cuts at: 1411 1411
Size: 4655
NspI r_CATG'y
Cuts at: 1708 2208 3811 1708
Size: 500 1603 2552
Fragments arranged by size:
2552 1603 500
PciI A'CATG_T
Cuts at: 3807 3807
Size: 4655
PflMI CCAn_nnn'nTGG
Cuts at: 860 860
Size: 4655
PmeI GTTT'AAAC
Cuts at: 1654 1654
Size: 4655
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3101 3133 3101
Size: 32 4623
PpuMI rG'GwC_Cy
Cuts at: 1179 1179
Size: 4655
PstI C_TGCA'G
Cuts at: 444 459 1702 2387 444
Size: 15 1243 685 2712
Fragments arranged by size:
2712 1243 685 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4655
PvuII CAG'CTG
Cuts at: 436 445 454 2080 2440 436
Size: 9 9 1626 360 2651
Fragments arranged by size:
2651 1626 360 9 9
RsrII CG'GwC_CG
Cuts at: 2850 2850
Size: 4655
SacI G_AGCT'C
Cuts at: 1669 1669
Size: 4655
SalI G'TCGA_C
Cuts at: 332 1692 332
Size: 1360 3295
SanDI GG'GwC_CC
Cuts at: 1179 1179
Size: 4655
SapI GCTCTTCn'nnn_
Cuts at: 2678 2888 2678
Size: 210 4445
SbfI CC_TGCA'GG
Cuts at: 1702 1702
Size: 4655
ScaI AGT'ACT
Cuts at: 737 4172 737
Size: 3435 1220
SfcI C'TryA_G
Cuts at: 33 277 440 455 527 1545 1698 2383 3351
Size: 244 163 15 72 1018 153 685 968
Cuts at: 3351 3542 33
Size: 191 1146
Fragments arranged by size:
1146 1018 968 685 244 191 163 153
72 15
SfoI GGC'GCC
Cuts at: 2087 2335 2087
Size: 248 4407
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4655
SmaI CCC'GGG
Cuts at: 1677 1677
Size: 4655
SmlI C'TyrA_G
Cuts at: 1810 2725 3439 3701 1810
Size: 915 714 262 2764
Fragments arranged by size:
2764 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4655
SphI G_CATG'C
Cuts at: 1708 2208 1708
Size: 500 4155
SspI AAT'ATT
Cuts at: 1565 1565
Size: 4655
StyI C'CwwG_G
Cuts at: 1143 1789 2143 1143
Size: 646 354 3655
Fragments arranged by size:
3655 646 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1412 2238 2371 2830 3039 1412
Size: 826 133 459 209 3028
Fragments arranged by size:
3028 826 459 209 133
TatI w'GTAC_w
Cuts at: 381 735 1084 1442 1503 4170 381
Size: 354 349 358 61 2667 866
Fragments arranged by size:
2667 866 358 354 349 61
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 687 719 4172 4204 687
Size: 32 3453 32 1138
Fragments arranged by size:
3453 1138 32 32
Tth111I GACn'n_nGTC
Cuts at: 2452 2452
Size: 4655
XbaI T'CTAG_A
Cuts at: 798 1686 798
Size: 888 3767
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2150 2150
Size: 4655
XmaI C'CCGG_G
Cuts at: 1675 1675
Size: 4655
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NheI NsiI NspI PciI PflMI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BglI BmgBI
Bpu10I BsgI BsiWI BsmBI BspEI BssHII BstXI BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
NcoI NdeI NotI NruI PacI PasI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI