Restriction map for
pF1KB5141
(Circular) (Six-base) MAPSORT of: pF1KB5141.seq Check: 5599 from: 1 to: 4682
pF1KB5141 4682 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 264 429 264
Size: 165 4517
AccI GT'mk_AC
Cuts at: 1720 1720
Size: 4682
Acc65I G'GTAC_C
Cuts at: 1698 1698
Size: 4682
AclI AA'CG_TT
Cuts at: 4522 4522
Size: 4682
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 1569 2507 2939 3686 3954 712
Size: 857 938 432 747 268 1440
Fragments arranged by size:
1440 938 857 747 432 268
AflIII A'CryG_T
Cuts at: 1022 1496 2034 3138 4115 1022
Size: 474 538 1104 977 1589
Fragments arranged by size:
1589 1104 977 538 474
AgeI A'CCGG_T
Cuts at: 3070 3070
Size: 4682
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1426 1426
Size: 4682
AlwNI CAG_nnn'CTG
Cuts at: 164 1246 1987 3554 164
Size: 1082 741 1567 1292
Fragments arranged by size:
1567 1292 1082 741
ApaI G_GGCC'C
Cuts at: 1082 1082
Size: 4682
ApaLI G'TGCA_C
Cuts at: 3452 3452
Size: 4682
ApoI r'AATT_y
Cuts at: 1394 1686 4035 4291 1394
Size: 292 2349 256 1785
Fragments arranged by size:
2349 1785 292 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4682
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4682
AvaI C'yCGr_G
Cuts at: 223 337 1081 1702 223
Size: 114 744 621 3203
Fragments arranged by size:
3203 744 621 114
BamHI G'GATC_C
Cuts at: 518 1707 518
Size: 1189 3493
BanI G'GyrC_C
Cuts at: 1698 2112 2360 2395 1698
Size: 414 248 35 3985
Fragments arranged by size:
3985 414 248 35
BanII G_rGCy'C
Cuts at: 338 785 851 1082 1106 1696 2726 338
Size: 447 66 231 24 590 1030 2294
Fragments arranged by size:
2294 1030 590 447 231 66 24
BbeI G_GCGC'C
Cuts at: 2116 2364 2116
Size: 248 4434
BbsI GAAGACnn'nnnn_
Cuts at: 1376 1376
Size: 4682
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1521 1555 90
Size: 34 1397 34 3217
Fragments arranged by size:
3217 1397 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2580 3341 4682 2580
Size: 761 1341 2580
Fragments arranged by size:
2580 1341 761
BclI T'GATC_A
Cuts at: 1175 2202 1175
Size: 1027 3655
BglI GCCn_nnn'nGGC
Cuts at: 196 196
Size: 4682
BglII A'GATC_T
Cuts at: 2197 4234 2197
Size: 2037 2645
BlpI GC'TnA_GC
Cuts at: 1794 1794
Size: 4682
Bme1580I G_kGCm'C
Cuts at: 513 1082 1237 1547 2307 2400 3456 513
Size: 569 155 310 760 93 1056 1739
Fragments arranged by size:
1739 1056 760 569 310 155 93
BmrI ACTGGGnnnn_n'
Cuts at: 1234 1894 1975 2310 4454 1234
Size: 660 81 335 2144 1462
Fragments arranged by size:
2144 1462 660 335 81
BmtI G_CTAG'C
Cuts at: 566 566
Size: 4682
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1125 3049 1125
Size: 1924 2758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 1016 1074 1475 1540 1589 1684 277
Size: 739 58 401 65 49 95 3275
Fragments arranged by size:
3275 739 401 95 65 58 49
Bpu10I CC'TnA_GC
Cuts at: 1472 1472
Size: 4682
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 301 1858 2737 3229 3527 196
Size: 105 1557 879 492 298 1351
Fragments arranged by size:
1557 1351 879 492 298 105
BsaI GGTCTCn'nnnn_
Cuts at: 4386 4386
Size: 4682
BsaAI yAC'GTr
Cuts at: 2035 2035
Size: 4682
BsaBI GATnn'nnATC
Cuts at: 6 742 1594 2196 4233 6
Size: 736 852 602 2037 455
Fragments arranged by size:
2037 852 736 602 455
BsaHI Gr'CG_yC
Cuts at: 398 788 2113 2361 3063 4559 398
Size: 390 1325 248 702 1496 521
Fragments arranged by size:
1496 1325 702 521 390 248
BsaWI w'CCGG_w
Cuts at: 1871 2392 3070 3344 3491 3902 1871
Size: 521 678 274 147 411 2651
Fragments arranged by size:
2651 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1108 1138 1108
Size: 30 4652
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 832 1172 1309 1537 832
Size: 340 137 228 3977
Fragments arranged by size:
3977 340 228 137
BseYI C'CCAG_C
Cuts at: 157 481 827 2107 2865 3442 3938 157
Size: 324 346 1280 758 577 496 901
Fragments arranged by size:
1280 901 758 577 496 346 324
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 883 1168 883
Size: 285 4397
BsiEI CG_ry'CG
Cuts at: 104 191 2224 2270 3478 104
Size: 87 2033 46 1208 1308
Fragments arranged by size:
2033 1308 1208 87 46
BsiHKAI G_wGCw'C
Cuts at: 212 317 338 785 851 899 1106 1696 2474
Size: 105 21 447 66 48 207 590 778
Cuts at: 2474 2664 3456 212
Size: 190 792 1438
Fragments arranged by size:
1438 792 778 590 447 207 190 105
66 48 21
BsmBI CGTCTCn'nnnn_
Cuts at: 708 708
Size: 4682
Bsp1286I G_dGCh'C
Cuts at: 212 317 338 513 785 851 899 1082 1106
Size: 105 21 175 272 66 48 183 24
Cuts at: 1106 1237 1547 1696 2307 2400 2474 2664 2726
Size: 131 310 149 611 93 74 190 62
Cuts at: 2726 3456 212
Size: 730 1438
Fragments arranged by size:
1438 730 611 310 272 190 183 175
149 131 105 93 74 66 62 48
24 21
BspEI T'CCGG_A
Cuts at: 3902 3902
Size: 4682
BspHI T'CATG_A
Cuts at: 3858 3858
Size: 4682
BspMI ACCTGCnnnn'nnnn_
Cuts at: 264 429 476 1190 1732 1946 2248 2629 264
Size: 165 47 714 542 214 302 381 2317
Fragments arranged by size:
2317 714 542 381 302 214 165 47
BsrBI CCG'CTC
Cuts at: 50 2974 3028 4512 4677 50
Size: 2924 54 1484 165 55
Fragments arranged by size:
2924 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2594 2594
Size: 4682
BsrFI r'CCGG_y
Cuts at: 233 322 2680 2861 3070 233
Size: 89 2358 181 209 1845
Fragments arranged by size:
2358 1845 209 181 89
BssSI C'ACGA_G
Cuts at: 2953 3311 2953
Size: 358 4324
BstAPI GCAn_nnn'nTGC
Cuts at: 947 2059 947
Size: 1112 3570
BstBI TT'CG_AA
Cuts at: 3043 3043
Size: 4682
BstXI CCAn_nnnn'nTGG
Cuts at: 1098 1461 1098
Size: 363 4319
BstYI r'GATC_y
Cuts at: 1 518 695 1439 1707 2174 2197 2532 2778
Size: 517 177 744 268 467 23 335 246
Cuts at: 2778 3790 3876 3888 4234 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 744 517 467 449 346 335 268
246 177 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 890 890
Size: 4682
BtgI C'CryG_G
Cuts at: 659 1259 659
Size: 600 4082
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2632 2813 2976 2632
Size: 181 163 4338
Fragments arranged by size:
4338 181 163
BtsI GCAGTG_nn'
Cuts at: 1398 1557 1925 1398
Size: 159 368 4155
Fragments arranged by size:
4155 368 159
DraI TTT'AAA
Cuts at: 1681 1681
Size: 4682
DrdI GACnn_nn'nnGTC
Cuts at: 2388 3246 2388
Size: 858 3824
EaeI y'GGCC_r
Cuts at: 188 1346 2267 2441 2832 2859 4204 188
Size: 1158 921 174 391 27 1345 666
Fragments arranged by size:
1345 1158 921 666 391 174 27
EagI C'GGCC_G
Cuts at: 188 2267 188
Size: 2079 2603
EarI CTCTTCn'nnn_
Cuts at: 974 2705 2915 974
Size: 1731 210 2741
Fragments arranged by size:
2741 1731 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 531 2890 3200 3346 4492 531
Size: 2359 310 146 1146 721
Fragments arranged by size:
2359 1146 721 310 146
EcoICRI GAG'CTC
Cuts at: 336 783 849 1104 1694 336
Size: 447 66 255 590 3324
Fragments arranged by size:
3324 590 447 255 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 712 1016 1074 1475 1540 1569 1589 1684
Size: 435 304 58 401 65 29 20 95
Cuts at: 1684 2507 2939 3686 3954 277
Size: 823 432 747 268 1005
Fragments arranged by size:
1005 823 747 435 432 401 304 268
95 65 58 29 20
EcoNI CCTnn'n_nnAGG
Cuts at: 436 814 436
Size: 378 4304
EcoO109I rG'GnC_Cy
Cuts at: 304 551 1078 1821 304
Size: 247 527 743 3165
Fragments arranged by size:
3165 743 527 247
EcoRI G'AATT_C
Cuts at: 1394 1686 1394
Size: 292 4390
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2701 2701
Size: 4682
FspI TGC'GCA
Cuts at: 2463 2463
Size: 4682
HaeII r_GCGC'y
Cuts at: 127 835 2116 2364 3386 4365 127
Size: 708 1281 248 1022 979 444
Fragments arranged by size:
1281 1022 979 708 444 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 374 406 1312 1344 2524 2554 2556 2586 374
Size: 32 906 32 1180 30 2 30 2470
Fragments arranged by size:
2470 1180 906 32 32 30 30 2
HincII GTy'rAC
Cuts at: 576 1721 576
Size: 1145 3537
KasI G'GCGC_C
Cuts at: 2112 2360 2112
Size: 248 4434
KpnI G_GTAC'C
Cuts at: 1702 1702
Size: 4682
MluI A'CGCG_T
Cuts at: 4115 4115
Size: 4682
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3353 3537 3353
Size: 184 4498
MscI TGG'CCA
Cuts at: 1348 2443 1348
Size: 1095 3587
MslI CAynn'nnrTG
Cuts at: 796 1171 1270 2671 2798 796
Size: 375 99 1401 127 2680
Fragments arranged by size:
2680 1401 375 127 99
MspA1I CmG'CkG
Cuts at: 114 348 769 1206 1793 2107 2467 3480 3725
Size: 234 421 437 587 314 360 1013 245
Cuts at: 3725 114
Size: 1071
Fragments arranged by size:
1071 1013 587 437 421 360 314 245
234
NaeI GCC'GGC
Cuts at: 235 324 2863 235
Size: 89 2539 2054
Fragments arranged by size:
2539 2054 89
NarI GG'CG_CC
Cuts at: 2113 2361 2113
Size: 248 4434
NcoI C'CATG_G
Cuts at: 1259 1259
Size: 4682
NgoMIV G'CCGG_C
Cuts at: 233 322 2861 233
Size: 89 2539 2054
Fragments arranged by size:
2539 2054 89
NheI G'CTAG_C
Cuts at: 562 562
Size: 4682
NotI GC'GGCC_GC
Cuts at: 188 188
Size: 4682
NspI r_CATG'y
Cuts at: 1500 1735 2235 3142 1500
Size: 235 500 907 3040
Fragments arranged by size:
3040 907 500 235
PasI CC'CwG_GG
Cuts at: 913 913
Size: 4682
PciI A'CATG_T
Cuts at: 1496 3138 1496
Size: 1642 3040
PflMI CCAn_nnn'nTGG
Cuts at: 164 408 1246 1485 164
Size: 244 838 239 3361
Fragments arranged by size:
3361 838 244 239
PfoI T'CCnGG_A
Cuts at: 1517 1661 1517
Size: 144 4538
PmeI GTTT'AAAC
Cuts at: 1681 1681
Size: 4682
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3853 3821
Size: 32 4650
PpuMI rG'GwC_Cy
Cuts at: 304 551 304
Size: 247 4435
PspOMI G'GGCC_C
Cuts at: 1078 1078
Size: 4682
PspXI vC'TCGA_Gb
Cuts at: 337 337
Size: 4682
PstI C_TGCA'G
Cuts at: 347 527 1634 1729 2414 347
Size: 180 1107 95 685 2615
Fragments arranged by size:
2615 1107 685 180 95
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4682
PvuII CAG'CTG
Cuts at: 769 1206 2107 2467 769
Size: 437 901 360 2984
Fragments arranged by size:
2984 901 437 360
RsrII CG'GwC_CG
Cuts at: 2877 2877
Size: 4682
SacI G_AGCT'C
Cuts at: 338 785 851 1106 1696 338
Size: 447 66 255 590 3324
Fragments arranged by size:
3324 590 447 255 66
SalI G'TCGA_C
Cuts at: 1719 1719
Size: 4682
SanDI GG'GwC_CC
Cuts at: 304 304
Size: 4682
SapI GCTCTTCn'nnn_
Cuts at: 2705 2915 2705
Size: 210 4472
SbfI CC_TGCA'GG
Cuts at: 527 1729 527
Size: 1202 3480
ScaI AGT'ACT
Cuts at: 1087 4199 1087
Size: 3112 1570
SfcI C'TryA_G
Cuts at: 33 343 523 555 1630 1725 2410 3403 3594
Size: 310 180 32 1075 95 685 993 191
Cuts at: 3594 33
Size: 1121
Fragments arranged by size:
1121 1075 993 685 310 191 180 95
32
SfiI GGCCn_nnn'nGGCC
Cuts at: 196 196
Size: 4682
SfoI GGC'GCC
Cuts at: 2114 2362 2114
Size: 248 4434
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4682
SmaI CCC'GGG
Cuts at: 1704 1704
Size: 4682
SmlI C'TyrA_G
Cuts at: 211 316 337 1837 2752 3244 3506 211
Size: 105 21 1500 915 492 262 1387
Fragments arranged by size:
1500 1387 915 492 262 105 21
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4682
SphI G_CATG'C
Cuts at: 1735 2235 1735
Size: 500 4182
StuI AGG'CCT
Cuts at: 199 199
Size: 4682
StyI C'CwwG_G
Cuts at: 1127 1259 1816 2170 1127
Size: 132 557 354 3639
Fragments arranged by size:
3639 557 354 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1320 2265 2398 2857 3066 1320
Size: 945 133 459 209 2936
Fragments arranged by size:
2936 945 459 209 133
TatI w'GTAC_w
Cuts at: 1025 1085 4197 1025
Size: 60 3112 1510
Fragments arranged by size:
3112 1510 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 296 328 1108 1140 4199 4231 296
Size: 32 780 32 3059 32 747
Fragments arranged by size:
3059 780 747 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1267 2479 1267
Size: 1212 3470
XbaI T'CTAG_A
Cuts at: 1713 1713
Size: 4682
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1134 2177 1134
Size: 1043 3639
XhoI C'TCGA_G
Cuts at: 337 337
Size: 4682
XmaI C'CCGG_G
Cuts at: 1702 1702
Size: 4682
XmnI GAAnn'nnTTC
Cuts at: 1645 1645
Size: 4682
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BsiWI BsmI BsrGI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NruI NsiI PacI PmlI
PshAI PsiI PsrI SacII SexAI SgrAI SnaBI SrfI
SspI SwaI ZraI