(Circular) (Six-base) MAPSORT of: pF1KB5154.seq Check: 3566 from: 1 to: 4997 pF1KB5154 4997 bp With 182 enzymes: SgfI * June 20, 2008 10:24 .. AatII G_ACGT'C Cuts at: 1562 1937 1562 Size: 375 4622 AccI GT'mk_AC Cuts at: 516 940 2035 516 Size: 424 1095 3478 Fragments arranged by size: 3478 1095 424 Acc65I G'GTAC_C Cuts at: 2013 2013 Size: 4997 AclI AA'CG_TT Cuts at: 4837 4837 Size: 4997 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 308 1607 1726 2822 3254 3607 4269 308 Size: 1299 119 1096 432 353 662 1036 Fragments arranged by size: 1299 1096 1036 662 432 353 119 AflIII A'CryG_T Cuts at: 2349 4149 4430 2349 Size: 1800 281 2916 Fragments arranged by size: 2916 1800 281 AlwNI CAG_nnn'CTG Cuts at: 130 1232 2302 3740 130 Size: 1102 1070 1438 1387 Fragments arranged by size: 1438 1387 1102 1070 ApaLI G'TGCA_C Cuts at: 3835 3835 Size: 4997 ApoI r'AATT_y Cuts at: 865 951 1471 1645 2001 4350 4606 865 Size: 86 520 174 356 2349 256 1256 Fragments arranged by size: 2349 1256 520 356 256 174 86 AseI AT'TA_AT Cuts at: 20 1577 20 Size: 1557 3440 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4997 AvaI C'yCGr_G Cuts at: 1171 2017 1171 Size: 846 4151 AvrII C'CTAG_G Cuts at: 1437 1437 Size: 4997 BamHI G'GATC_C Cuts at: 2022 2022 Size: 4997 BanI G'GyrC_C Cuts at: 145 1363 1906 2013 2427 2675 2710 145 Size: 1218 543 107 414 248 35 2432 Fragments arranged by size: 2432 1218 543 414 248 107 35 BanII G_rGCy'C Cuts at: 272 409 1275 2011 3041 272 Size: 137 866 736 1030 2228 Fragments arranged by size: 2228 1030 866 736 137 BbeI G_GCGC'C Cuts at: 1910 2431 2679 1910 Size: 521 248 4228 Fragments arranged by size: 4228 521 248 BbsI GAAGACnn'nnnn_ Cuts at: 676 1595 676 Size: 919 4078 BciVI GTATCCnnnnn_n' Cuts at: 369 387 1868 2895 3951 4997 369 Size: 18 1481 1027 1056 1046 369 Fragments arranged by size: 1481 1056 1046 1027 369 18 BclI T'GATC_A Cuts at: 2517 2517 Size: 4997 BglI GCCn_nnn'nGGC Cuts at: 112 112 Size: 4997 BglII A'GATC_T Cuts at: 1286 2512 4549 1286 Size: 1226 2037 1734 Fragments arranged by size: 2037 1734 1226 BlpI GC'TnA_GC Cuts at: 1073 1145 2109 1073 Size: 72 964 3961 Fragments arranged by size: 3961 964 72 Bme1580I G_kGCm'C Cuts at: 2622 2715 3839 2622 Size: 93 1124 3780 Fragments arranged by size: 3780 1124 93 BmrI ACTGGGnnnn_n' Cuts at: 266 729 1272 1429 2209 2290 2625 4769 266 Size: 463 543 157 780 81 335 2144 494 Fragments arranged by size: 2144 780 543 494 463 335 157 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 264 1361 3364 264 Size: 1097 2003 1897 Fragments arranged by size: 2003 1897 1097 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 257 1235 257 Size: 978 4019 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2173 3052 3766 4064 2173 Size: 879 714 298 3106 Fragments arranged by size: 3106 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 738 1211 4701 738 Size: 473 3490 1034 Fragments arranged by size: 3490 1034 473 BsaAI yAC'GTr Cuts at: 1947 2350 1947 Size: 403 4594 BsaBI GATnn'nnATC Cuts at: 6 662 1127 2511 4548 6 Size: 656 465 1384 2037 455 Fragments arranged by size: 2037 1384 656 465 455 BsaHI Gr'CG_yC Cuts at: 1559 1907 1934 2428 2676 3378 4874 1559 Size: 348 27 494 248 702 1496 1682 Fragments arranged by size: 1682 1496 702 494 348 248 27 BsaWI w'CCGG_w Cuts at: 584 2186 2707 3385 3796 3943 4217 584 Size: 1602 521 678 411 147 274 1364 Fragments arranged by size: 1602 1364 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1959 1989 1959 Size: 30 4967 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 221 1010 221 Size: 789 4208 BseYI C'CCAG_C Cuts at: 370 1193 1430 1797 1901 2422 3180 3845 4253 Size: 823 237 367 104 521 758 665 408 Cuts at: 4253 370 Size: 1114 Fragments arranged by size: 1114 823 758 665 521 408 367 237 104 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1806 1806 Size: 4997 BsiEI CG_ry'CG Cuts at: 104 170 2539 2585 3815 104 Size: 66 2369 46 1230 1286 Fragments arranged by size: 2369 1286 1230 66 46 BsiHKAI G_wGCw'C Cuts at: 1275 1922 2011 2789 2979 3839 1275 Size: 647 89 778 190 860 2433 Fragments arranged by size: 2433 860 778 647 190 89 BsmBI CGTCTCn'nnnn_ Cuts at: 1550 1550 Size: 4997 Bsp1286I G_dGCh'C Cuts at: 272 409 1275 1922 2011 2622 2715 2789 2979 Size: 137 866 647 89 611 93 74 190 Cuts at: 2979 3041 3839 272 Size: 62 798 1430 Fragments arranged by size: 1430 866 798 647 611 190 137 93 89 74 62 BspEI T'CCGG_A Cuts at: 584 584 Size: 4997 BspHI T'CATG_A Cuts at: 524 3429 524 Size: 2905 2092 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2047 2261 2563 2944 2047 Size: 214 302 381 4100 Fragments arranged by size: 4100 381 302 214 BsrBI CCG'CTC Cuts at: 50 166 229 3289 3343 4827 4992 50 Size: 116 63 3060 54 1484 165 55 Fragments arranged by size: 3060 1484 165 116 63 55 54 BsrDI GCAATG_nn' Cuts at: 460 543 1609 2909 460 Size: 83 1066 1300 2548 Fragments arranged by size: 2548 1300 1066 83 BsrFI r'CCGG_y Cuts at: 207 1298 2995 3176 207 Size: 1091 1697 181 2028 Fragments arranged by size: 2028 1697 1091 181 BsrGI T'GTAC_A Cuts at: 466 1811 466 Size: 1345 3652 BssSI C'ACGA_G Cuts at: 3268 3976 3268 Size: 708 4289 BstAPI GCAn_nnn'nTGC Cuts at: 554 2374 554 Size: 1820 3177 BstBI TT'CG_AA Cuts at: 3358 3358 Size: 4997 BstXI CCAn_nnnn'nTGG Cuts at: 565 1522 565 Size: 957 4040 BstYI r'GATC_y Cuts at: 1 245 828 843 1286 1869 2022 2489 2512 Size: 244 583 15 443 583 153 467 23 Cuts at: 2512 2847 3093 3399 3411 3497 4549 1 Size: 335 246 306 12 86 1052 449 Fragments arranged by size: 1052 583 583 467 449 443 335 306 246 244 153 86 23 15 12 BtgI C'CryG_G Cuts at: 107 410 1910 1981 107 Size: 303 1500 71 3123 Fragments arranged by size: 3123 1500 303 71 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2947 3128 3291 2947 Size: 181 163 4653 Fragments arranged by size: 4653 181 163 BtsI GCAGTG_nn' Cuts at: 936 2240 936 Size: 1304 3693 DraI TTT'AAA Cuts at: 956 1996 956 Size: 1040 3957 DrdI GACnn_nn'nnGTC Cuts at: 642 1605 2703 4047 642 Size: 963 1098 1344 1592 Fragments arranged by size: 1592 1344 1098 963 EaeI y'GGCC_r Cuts at: 132 426 626 763 2582 2756 3147 3174 4519 Size: 294 200 137 1819 174 391 27 1345 Cuts at: 4519 132 Size: 610 Fragments arranged by size: 1819 1345 610 391 294 200 174 137 27 EagI C'GGCC_G Cuts at: 2582 2582 Size: 4997 EarI CTCTTCn'nnn_ Cuts at: 185 413 1542 1693 3020 3230 185 Size: 228 1129 151 1327 210 1952 Fragments arranged by size: 1952 1327 1129 228 210 151 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1503 3205 3947 4093 4807 1503 Size: 1702 742 146 714 1693 Fragments arranged by size: 1702 1693 742 714 146 EcoICRI GAG'CTC Cuts at: 1273 2009 1273 Size: 736 4261 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 257 308 1235 1607 1726 2822 3254 3607 4269 Size: 51 927 372 119 1096 432 353 662 Cuts at: 4269 257 Size: 985 Fragments arranged by size: 1096 985 927 662 432 372 353 119 51 EcoNI CCTnn'n_nnAGG Cuts at: 158 158 Size: 4997 EcoO109I rG'GnC_Cy Cuts at: 127 885 1340 2136 127 Size: 758 455 796 2988 Fragments arranged by size: 2988 796 758 455 EcoRI G'AATT_C Cuts at: 2001 2001 Size: 4997 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3016 3016 Size: 4997 FspI TGC'GCA Cuts at: 2778 2778 Size: 4997 HaeII r_GCGC'y Cuts at: 1080 1910 2431 2679 3909 4680 1080 Size: 830 521 248 1230 771 1397 Fragments arranged by size: 1397 1230 830 771 521 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 230 262 322 354 381 413 493 525 1959 Size: 32 60 32 27 32 80 32 1434 Cuts at: 1959 1991 2839 2869 2871 2901 230 Size: 32 848 30 2 30 2326 Fragments arranged by size: 2326 1434 848 80 60 32 32 32 32 32 30 30 27 2 HincII GTy'rAC Cuts at: 2036 2036 Size: 4997 KasI G'GCGC_C Cuts at: 1906 2427 2675 1906 Size: 521 248 4228 Fragments arranged by size: 4228 521 248 KpnI G_GTAC'C Cuts at: 2017 2017 Size: 4997 MluI A'CGCG_T Cuts at: 4430 4430 Size: 4997 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 283 1293 3756 3940 283 Size: 1010 2463 184 1340 Fragments arranged by size: 2463 1340 1010 184 MscI TGG'CCA Cuts at: 134 628 765 2758 134 Size: 494 137 1993 2373 Fragments arranged by size: 2373 1993 494 137 MslI CAynn'nnrTG Cuts at: 563 2986 3113 563 Size: 2423 127 2447 Fragments arranged by size: 2447 2423 127 MspA1I CmG'CkG Cuts at: 217 983 1411 2108 2422 2782 3566 3811 217 Size: 766 428 697 314 360 784 245 1403 Fragments arranged by size: 1403 784 766 697 428 360 314 245 NaeI GCC'GGC Cuts at: 3178 3178 Size: 4997 NarI GG'CG_CC Cuts at: 1907 2428 2676 1907 Size: 521 248 4228 Fragments arranged by size: 4228 521 248 NcoI C'CATG_G Cuts at: 107 1981 107 Size: 1874 3123 NgoMIV G'CCGG_C Cuts at: 3176 3176 Size: 4997 NspI r_CATG'y Cuts at: 2050 2550 4153 2050 Size: 500 1603 2894 Fragments arranged by size: 2894 1603 500 PciI A'CATG_T Cuts at: 4149 4149 Size: 4997 PfoI T'CCnGG_A Cuts at: 234 1212 234 Size: 978 4019 PmeI GTTT'AAAC Cuts at: 1996 1996 Size: 4997 PmlI CAC'GTG Cuts at: 1947 1947 Size: 4997 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3443 3475 3443 Size: 32 4965 PpuMI rG'GwC_Cy Cuts at: 127 885 127 Size: 758 4239 PsiI TTA'TAA Cuts at: 1739 1739 Size: 4997 PstI C_TGCA'G Cuts at: 2044 2729 2044 Size: 685 4312 PvuI CG_AT'CG Cuts at: 104 104 Size: 4997 PvuII CAG'CTG Cuts at: 2422 2782 2422 Size: 360 4637 RsrII CG'GwC_CG Cuts at: 3192 3192 Size: 4997 SacI G_AGCT'C Cuts at: 1275 2011 1275 Size: 736 4261 SalI G'TCGA_C Cuts at: 2034 2034 Size: 4997 SapI GCTCTTCn'nnn_ Cuts at: 1693 3020 3230 1693 Size: 1327 210 3460 Fragments arranged by size: 3460 1327 210 SbfI CC_TGCA'GG Cuts at: 2044 2044 Size: 4997 ScaI AGT'ACT Cuts at: 4514 4514 Size: 4997 SfcI C'TryA_G Cuts at: 33 534 1224 2040 2725 3693 3884 33 Size: 501 690 816 685 968 191 1146 Fragments arranged by size: 1146 968 816 690 685 501 191 SfoI GGC'GCC Cuts at: 1908 2429 2677 1908 Size: 521 248 4228 Fragments arranged by size: 4228 521 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4997 SmaI CCC'GGG Cuts at: 2019 2019 Size: 4997 SmlI C'TyrA_G Cuts at: 2152 3067 3781 4043 2152 Size: 915 714 262 3106 Fragments arranged by size: 3106 915 714 262 SpeI A'CTAG_T Cuts at: 67 943 67 Size: 876 4121 SphI G_CATG'C Cuts at: 2050 2550 2050 Size: 500 4497 SspI AAT'ATT Cuts at: 1733 1733 Size: 4997 StyI C'CwwG_G Cuts at: 107 1437 1981 2131 2485 107 Size: 1330 544 150 354 2619 Fragments arranged by size: 2619 1330 544 354 150 SwaI ATTT'AAAT Cuts at: 956 956 Size: 4997 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 427 839 2580 2713 3172 3381 427 Size: 412 1741 133 459 209 2043 Fragments arranged by size: 2043 1741 459 412 209 133 TatI w'GTAC_w Cuts at: 466 1811 4512 466 Size: 1345 2701 951 Fragments arranged by size: 2701 1345 951 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4514 4546 4514 Size: 32 4965 Tth111I GACn'n_nGTC Cuts at: 2794 2794 Size: 4997 XbaI T'CTAG_A Cuts at: 2028 2028 Size: 4997 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1519 2492 1519 Size: 973 4024 XmaI C'CCGG_G Cuts at: 2017 2017 Size: 4997 ZraI GAC'GTC Cuts at: 1560 1935 1560 Size: 375 4622 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI PmlI PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AarI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI AscI BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI XhoI XmnI