Restriction map for pF1KB5154
 (Circular) (Six-base) MAPSORT of: pF1KB5154.seq  Check: 3566  from: 1  to: 4997



pF1KB5154  4997 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:24  ..

AatII G_ACGT'C

Cuts at:   1562    1937    1562 
   Size:        375    4622
 
AccI GT'mk_AC

Cuts at:    516     940    2035     516 
   Size:        424    1095    3478

  Fragments arranged by size: 

                3478    1095     424
 
Acc65I G'GTAC_C

Cuts at:   2013    2013 
   Size:       4997
 
AclI AA'CG_TT

Cuts at:   4837    4837 
   Size:       4997
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    308    1607    1726    2822    3254    3607    4269     308 
   Size:       1299     119    1096     432     353     662    1036

  Fragments arranged by size: 

                1299    1096    1036     662     432     353     119
 
AflIII A'CryG_T

Cuts at:   2349    4149    4430    2349 
   Size:       1800     281    2916

  Fragments arranged by size: 

                2916    1800     281
 
AlwNI CAG_nnn'CTG

Cuts at:    130    1232    2302    3740     130 
   Size:       1102    1070    1438    1387

  Fragments arranged by size: 

                1438    1387    1102    1070
 
ApaLI G'TGCA_C

Cuts at:   3835    3835 
   Size:       4997
 
ApoI r'AATT_y

Cuts at:    865     951    1471    1645    2001    4350    4606     865 
   Size:         86     520     174     356    2349     256    1256

  Fragments arranged by size: 

                2349    1256     520     356     256     174      86
 
AseI AT'TA_AT

Cuts at:     20    1577      20 
   Size:       1557    3440
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4997
 
AvaI C'yCGr_G

Cuts at:   1171    2017    1171 
   Size:        846    4151
 
AvrII C'CTAG_G

Cuts at:   1437    1437 
   Size:       4997
 
BamHI G'GATC_C

Cuts at:   2022    2022 
   Size:       4997
 
BanI G'GyrC_C

Cuts at:    145    1363    1906    2013    2427    2675    2710     145 
   Size:       1218     543     107     414     248      35    2432

  Fragments arranged by size: 

                2432    1218     543     414     248     107      35
 
BanII G_rGCy'C

Cuts at:    272     409    1275    2011    3041     272 
   Size:        137     866     736    1030    2228

  Fragments arranged by size: 

                2228    1030     866     736     137
 
BbeI G_GCGC'C

Cuts at:   1910    2431    2679    1910 
   Size:        521     248    4228

  Fragments arranged by size: 

                4228     521     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    676    1595     676 
   Size:        919    4078
 
BciVI GTATCCnnnnn_n'

Cuts at:    369     387    1868    2895    3951    4997     369 
   Size:         18    1481    1027    1056    1046     369

  Fragments arranged by size: 

                1481    1056    1046    1027     369      18
 
BclI T'GATC_A

Cuts at:   2517    2517 
   Size:       4997
 
BglI GCCn_nnn'nGGC

Cuts at:    112     112 
   Size:       4997
 
BglII A'GATC_T

Cuts at:   1286    2512    4549    1286 
   Size:       1226    2037    1734

  Fragments arranged by size: 

                2037    1734    1226
 
BlpI GC'TnA_GC

Cuts at:   1073    1145    2109    1073 
   Size:         72     964    3961

  Fragments arranged by size: 

                3961     964      72
 
Bme1580I G_kGCm'C

Cuts at:   2622    2715    3839    2622 
   Size:         93    1124    3780

  Fragments arranged by size: 

                3780    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    266     729    1272    1429    2209    2290    2625    4769     266 
   Size:        463     543     157     780      81     335    2144     494

  Fragments arranged by size: 

                2144     780     543     494     463     335     157      81
            
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    264    1361    3364     264 
   Size:       1097    2003    1897

  Fragments arranged by size: 

                2003    1897    1097
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    257    1235     257 
   Size:        978    4019
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2173    3052    3766    4064    2173 
   Size:        879     714     298    3106

  Fragments arranged by size: 

                3106     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    738    1211    4701     738 
   Size:        473    3490    1034

  Fragments arranged by size: 

                3490    1034     473
 
BsaAI yAC'GTr

Cuts at:   1947    2350    1947 
   Size:        403    4594
 
BsaBI GATnn'nnATC

Cuts at:      6     662    1127    2511    4548       6 
   Size:        656     465    1384    2037     455

  Fragments arranged by size: 

                2037    1384     656     465     455
 
BsaHI Gr'CG_yC

Cuts at:   1559    1907    1934    2428    2676    3378    4874    1559 
   Size:        348      27     494     248     702    1496    1682

  Fragments arranged by size: 

                1682    1496     702     494     348     248      27
 
BsaWI w'CCGG_w

Cuts at:    584    2186    2707    3385    3796    3943    4217     584 
   Size:       1602     521     678     411     147     274    1364

  Fragments arranged by size: 

                1602    1364     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1959    1989    1959 
   Size:         30    4967
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    221    1010     221 
   Size:        789    4208
 
BseYI C'CCAG_C

Cuts at:    370    1193    1430    1797    1901    2422    3180    3845    4253 
   Size:        823     237     367     104     521     758     665     408

Cuts at:   4253     370 
   Size:       1114

  Fragments arranged by size: 

                1114     823     758     665     521     408     367     237
                 104
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1806    1806 
   Size:       4997
 
BsiEI CG_ry'CG

Cuts at:    104     170    2539    2585    3815     104 
   Size:         66    2369      46    1230    1286

  Fragments arranged by size: 

                2369    1286    1230      66      46
 
BsiHKAI G_wGCw'C

Cuts at:   1275    1922    2011    2789    2979    3839    1275 
   Size:        647      89     778     190     860    2433

  Fragments arranged by size: 

                2433     860     778     647     190      89
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1550    1550 
   Size:       4997
 
Bsp1286I G_dGCh'C

Cuts at:    272     409    1275    1922    2011    2622    2715    2789    2979 
   Size:        137     866     647      89     611      93      74     190

Cuts at:   2979    3041    3839     272 
   Size:         62     798    1430

  Fragments arranged by size: 

                1430     866     798     647     611     190     137      93
                  89      74      62
 
BspEI T'CCGG_A

Cuts at:    584     584 
   Size:       4997
 
BspHI T'CATG_A

Cuts at:    524    3429     524 
   Size:       2905    2092
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2047    2261    2563    2944    2047 
   Size:        214     302     381    4100

  Fragments arranged by size: 

                4100     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     166     229    3289    3343    4827    4992      50 
   Size:        116      63    3060      54    1484     165      55

  Fragments arranged by size: 

                3060    1484     165     116      63      55      54
 
BsrDI GCAATG_nn'

Cuts at:    460     543    1609    2909     460 
   Size:         83    1066    1300    2548

  Fragments arranged by size: 

                2548    1300    1066      83
 
BsrFI r'CCGG_y

Cuts at:    207    1298    2995    3176     207 
   Size:       1091    1697     181    2028

  Fragments arranged by size: 

                2028    1697    1091     181
 
BsrGI T'GTAC_A

Cuts at:    466    1811     466 
   Size:       1345    3652
 
BssSI C'ACGA_G

Cuts at:   3268    3976    3268 
   Size:        708    4289
 
BstAPI GCAn_nnn'nTGC

Cuts at:    554    2374     554 
   Size:       1820    3177
 
BstBI TT'CG_AA

Cuts at:   3358    3358 
   Size:       4997
 
BstXI CCAn_nnnn'nTGG

Cuts at:    565    1522     565 
   Size:        957    4040
 
BstYI r'GATC_y

Cuts at:      1     245     828     843    1286    1869    2022    2489    2512 
   Size:        244     583      15     443     583     153     467      23

Cuts at:   2512    2847    3093    3399    3411    3497    4549       1 
   Size:        335     246     306      12      86    1052     449

  Fragments arranged by size: 

                1052     583     583     467     449     443     335     306
                 246     244     153      86      23      15      12
 
BtgI C'CryG_G

Cuts at:    107     410    1910    1981     107 
   Size:        303    1500      71    3123

  Fragments arranged by size: 

                3123    1500     303      71
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2947    3128    3291    2947 
   Size:        181     163    4653

  Fragments arranged by size: 

                4653     181     163
 
BtsI GCAGTG_nn'

Cuts at:    936    2240     936 
   Size:       1304    3693
 
DraI TTT'AAA

Cuts at:    956    1996     956 
   Size:       1040    3957
 
DrdI GACnn_nn'nnGTC

Cuts at:    642    1605    2703    4047     642 
   Size:        963    1098    1344    1592

  Fragments arranged by size: 

                1592    1344    1098     963
 
EaeI y'GGCC_r

Cuts at:    132     426     626     763    2582    2756    3147    3174    4519 
   Size:        294     200     137    1819     174     391      27    1345

Cuts at:   4519     132 
   Size:        610

  Fragments arranged by size: 

                1819    1345     610     391     294     200     174     137
                  27
 
EagI C'GGCC_G

Cuts at:   2582    2582 
   Size:       4997
 
EarI CTCTTCn'nnn_

Cuts at:    185     413    1542    1693    3020    3230     185 
   Size:        228    1129     151    1327     210    1952

  Fragments arranged by size: 

                1952    1327    1129     228     210     151
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1503    3205    3947    4093    4807    1503 
   Size:       1702     742     146     714    1693

  Fragments arranged by size: 

                1702    1693     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   1273    2009    1273 
   Size:        736    4261
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    257     308    1235    1607    1726    2822    3254    3607    4269 
   Size:         51     927     372     119    1096     432     353     662

Cuts at:   4269     257 
   Size:        985

  Fragments arranged by size: 

                1096     985     927     662     432     372     353     119
                  51
 
EcoNI CCTnn'n_nnAGG

Cuts at:    158     158 
   Size:       4997
 
EcoO109I rG'GnC_Cy

Cuts at:    127     885    1340    2136     127 
   Size:        758     455     796    2988

  Fragments arranged by size: 

                2988     796     758     455
 
EcoRI G'AATT_C

Cuts at:   2001    2001 
   Size:       4997
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3016    3016 
   Size:       4997
 
FspI TGC'GCA

Cuts at:   2778    2778 
   Size:       4997
 
HaeII r_GCGC'y

Cuts at:   1080    1910    2431    2679    3909    4680    1080 
   Size:        830     521     248    1230     771    1397

  Fragments arranged by size: 

                1397    1230     830     771     521     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    230     262     322     354     381     413     493     525    1959 
   Size:         32      60      32      27      32      80      32    1434

Cuts at:   1959    1991    2839    2869    2871    2901     230 
   Size:         32     848      30       2      30    2326

  Fragments arranged by size: 

                2326    1434     848      80      60      32      32      32
                  32      32      30      30      27       2
 
HincII GTy'rAC

Cuts at:   2036    2036 
   Size:       4997
 
KasI G'GCGC_C

Cuts at:   1906    2427    2675    1906 
   Size:        521     248    4228

  Fragments arranged by size: 

                4228     521     248
 
KpnI G_GTAC'C

Cuts at:   2017    2017 
   Size:       4997
 
MluI A'CGCG_T

Cuts at:   4430    4430 
   Size:       4997
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    283    1293    3756    3940     283 
   Size:       1010    2463     184    1340

  Fragments arranged by size: 

                2463    1340    1010     184
 
MscI TGG'CCA

Cuts at:    134     628     765    2758     134 
   Size:        494     137    1993    2373

  Fragments arranged by size: 

                2373    1993     494     137
 
MslI CAynn'nnrTG

Cuts at:    563    2986    3113     563 
   Size:       2423     127    2447

  Fragments arranged by size: 

                2447    2423     127
 
MspA1I CmG'CkG

Cuts at:    217     983    1411    2108    2422    2782    3566    3811     217 
   Size:        766     428     697     314     360     784     245    1403

  Fragments arranged by size: 

                1403     784     766     697     428     360     314     245
            
 
NaeI GCC'GGC

Cuts at:   3178    3178 
   Size:       4997
 
NarI GG'CG_CC

Cuts at:   1907    2428    2676    1907 
   Size:        521     248    4228

  Fragments arranged by size: 

                4228     521     248
 
NcoI C'CATG_G

Cuts at:    107    1981     107 
   Size:       1874    3123
 
NgoMIV G'CCGG_C

Cuts at:   3176    3176 
   Size:       4997
 
NspI r_CATG'y

Cuts at:   2050    2550    4153    2050 
   Size:        500    1603    2894

  Fragments arranged by size: 

                2894    1603     500
 
PciI A'CATG_T

Cuts at:   4149    4149 
   Size:       4997
 
PfoI T'CCnGG_A

Cuts at:    234    1212     234 
   Size:        978    4019
 
PmeI GTTT'AAAC

Cuts at:   1996    1996 
   Size:       4997
 
PmlI CAC'GTG

Cuts at:   1947    1947 
   Size:       4997
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3443    3475    3443 
   Size:         32    4965
 
PpuMI rG'GwC_Cy

Cuts at:    127     885     127 
   Size:        758    4239
 
PsiI TTA'TAA

Cuts at:   1739    1739 
   Size:       4997
 
PstI C_TGCA'G

Cuts at:   2044    2729    2044 
   Size:        685    4312
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4997
 
PvuII CAG'CTG

Cuts at:   2422    2782    2422 
   Size:        360    4637
 
RsrII CG'GwC_CG

Cuts at:   3192    3192 
   Size:       4997
 
SacI G_AGCT'C

Cuts at:   1275    2011    1275 
   Size:        736    4261
 
SalI G'TCGA_C

Cuts at:   2034    2034 
   Size:       4997
 
SapI GCTCTTCn'nnn_

Cuts at:   1693    3020    3230    1693 
   Size:       1327     210    3460

  Fragments arranged by size: 

                3460    1327     210
 
SbfI CC_TGCA'GG

Cuts at:   2044    2044 
   Size:       4997
 
ScaI AGT'ACT

Cuts at:   4514    4514 
   Size:       4997
 
SfcI C'TryA_G

Cuts at:     33     534    1224    2040    2725    3693    3884      33 
   Size:        501     690     816     685     968     191    1146

  Fragments arranged by size: 

                1146     968     816     690     685     501     191
 
SfoI GGC'GCC

Cuts at:   1908    2429    2677    1908 
   Size:        521     248    4228

  Fragments arranged by size: 

                4228     521     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4997
 
SmaI CCC'GGG

Cuts at:   2019    2019 
   Size:       4997
 
SmlI C'TyrA_G

Cuts at:   2152    3067    3781    4043    2152 
   Size:        915     714     262    3106

  Fragments arranged by size: 

                3106     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67     943      67 
   Size:        876    4121
 
SphI G_CATG'C

Cuts at:   2050    2550    2050 
   Size:        500    4497
 
SspI AAT'ATT

Cuts at:   1733    1733 
   Size:       4997
 
StyI C'CwwG_G

Cuts at:    107    1437    1981    2131    2485     107 
   Size:       1330     544     150     354    2619

  Fragments arranged by size: 

                2619    1330     544     354     150
 
SwaI ATTT'AAAT

Cuts at:    956     956 
   Size:       4997
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    427     839    2580    2713    3172    3381     427 
   Size:        412    1741     133     459     209    2043

  Fragments arranged by size: 

                2043    1741     459     412     209     133
 
TatI w'GTAC_w

Cuts at:    466    1811    4512     466 
   Size:       1345    2701     951

  Fragments arranged by size: 

                2701    1345     951
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4514    4546    4514 
   Size:         32    4965
 
Tth111I GACn'n_nGTC

Cuts at:   2794    2794 
   Size:       4997
 
XbaI T'CTAG_A

Cuts at:   2028    2028 
   Size:       4997
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1519    2492    1519 
   Size:        973    4024
 
XmaI C'CCGG_G

Cuts at:   2017    2017 
   Size:       4997
 
ZraI GAC'GTC

Cuts at:   1560    1935    1560 
   Size:        375    4622
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI     PsiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     SspI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AgeI     AhdI     AleI     AlfI     AloI
     ApaI     AscI     BaeI    BbvCI     BcgI    BfrBI    BmgBI     BmtI
   Bpu10I    BsiWI     BsmI   BssHII   BstEII  BstZ17I   Bsu36I     ClaI
    CspCI   DraIII    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
    PshAI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     StuI     XhoI     XmnI
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