Restriction map for
pF1KB5155
(Circular) (Six-base) MAPSORT of: pF1KB5155.seq Check: 36 from: 1 to: 4646
pF1KB5155 4646 bp
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AccI GT'mk_AC
Cuts at: 1582 1684 1582
Size: 102 4544
Acc65I G'GTAC_C
Cuts at: 1662 1662
Size: 4646
AclI AA'CG_TT
Cuts at: 4486 4486
Size: 4646
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2471 2903 3256 3918 2471
Size: 432 353 662 3199
Fragments arranged by size:
3199 662 432 353
AflIII A'CryG_T
Cuts at: 1998 3798 4079 1998
Size: 1800 281 2565
Fragments arranged by size:
2565 1800 281
AleI CACnn'nnGTG
Cuts at: 853 1529 853
Size: 676 3970
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 817 849 817
Size: 32 4614
AlwNI CAG_nnn'CTG
Cuts at: 1951 3389 1951
Size: 1438 3208
ApaLI G'TGCA_C
Cuts at: 3484 3484
Size: 4646
ApoI r'AATT_y
Cuts at: 1333 1650 3999 4255 1333
Size: 317 2349 256 1724
Fragments arranged by size:
2349 1724 317 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4646
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4646
AvaI C'yCGr_G
Cuts at: 1666 1666
Size: 4646
BamHI G'GATC_C
Cuts at: 1671 1671
Size: 4646
BanI G'GyrC_C
Cuts at: 1504 1662 2076 2324 2359 1504
Size: 158 414 248 35 3791
Fragments arranged by size:
3791 414 248 158 35
BanII G_rGCy'C
Cuts at: 164 684 1660 2690 164
Size: 520 976 1030 2120
Fragments arranged by size:
2120 1030 976 520
BbeI G_GCGC'C
Cuts at: 2080 2328 2080
Size: 248 4398
BbvCI CC'TCA_GC
Cuts at: 955 955
Size: 4646
BciVI GTATCCnnnnn_n'
Cuts at: 2544 3600 4646 2544
Size: 1056 1046 2544
Fragments arranged by size:
2544 1056 1046
BclI T'GATC_A
Cuts at: 2166 2166
Size: 4646
BglI GCCn_nnn'nGGC
Cuts at: 277 277
Size: 4646
BglII A'GATC_T
Cuts at: 541 1410 2161 4198 541
Size: 869 751 2037 989
Fragments arranged by size:
2037 989 869 751
BlpI GC'TnA_GC
Cuts at: 1758 1758
Size: 4646
Bme1580I G_kGCm'C
Cuts at: 2271 2364 3488 2271
Size: 93 1124 3429
Fragments arranged by size:
3429 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 129 851 1858 1939 2274 4418 129
Size: 722 1007 81 335 2144 357
Fragments arranged by size:
2144 1007 722 357 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3013 3013
Size: 4646
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 757 757
Size: 4646
Bpu10I CC'TnA_GC
Cuts at: 955 955
Size: 4646
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1822 2701 3415 3713 1822
Size: 879 714 298 2755
Fragments arranged by size:
2755 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4350 4350
Size: 4646
BsaAI yAC'GTr
Cuts at: 1999 1999
Size: 4646
BsaBI GATnn'nnATC
Cuts at: 6 2160 4197 6
Size: 2154 2037 455
Fragments arranged by size:
2154 2037 455
BsaHI Gr'CG_yC
Cuts at: 2077 2325 3027 4523 2077
Size: 248 702 1496 2200
Fragments arranged by size:
2200 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1835 2356 3034 3445 3592 3866 1835
Size: 521 678 411 147 274 2615
Fragments arranged by size:
2615 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 347 377 1296 1326 347
Size: 30 919 30 3667
Fragments arranged by size:
3667 919 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 109 368 1529 109
Size: 259 1161 3226
Fragments arranged by size:
3226 1161 259
BseYI C'CCAG_C
Cuts at: 156 197 2071 2829 3494 3902 156
Size: 41 1874 758 665 408 900
Fragments arranged by size:
1874 900 758 665 408 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 901
Size: 4646
BsiEI CG_ry'CG
Cuts at: 104 2188 2234 3464 104
Size: 2084 46 1230 1286
Fragments arranged by size:
2084 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 684 850 1660 2438 2628 3488 684
Size: 166 810 778 190 860 1842
Fragments arranged by size:
1842 860 810 778 190 166
BsmI GAATG_Cn'
Cuts at: 601 601
Size: 4646
Bsp1286I G_dGCh'C
Cuts at: 164 684 850 1660 2271 2364 2438 2628 2690
Size: 520 166 810 611 93 74 190 62
Cuts at: 2690 3488 164
Size: 798 1322
Fragments arranged by size:
1322 810 798 611 520 190 166 93
74 62
BspHI T'CATG_A
Cuts at: 3078 3078
Size: 4646
BspMI ACCTGCnnnn'nnnn_
Cuts at: 836 1696 1910 2212 2593 836
Size: 860 214 302 381 2889
Fragments arranged by size:
2889 860 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2938 2992 4476 4641 50
Size: 2888 54 1484 165 55
Fragments arranged by size:
2888 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 809 1289 2558 809
Size: 480 1269 2897
Fragments arranged by size:
2897 1269 480
BsrFI r'CCGG_y
Cuts at: 2644 2825 2644
Size: 181 4465
BssSI C'ACGA_G
Cuts at: 2917 3625 2917
Size: 708 3938
BstAPI GCAn_nnn'nTGC
Cuts at: 2023 2023
Size: 4646
BstBI TT'CG_AA
Cuts at: 3007 3007
Size: 4646
BstXI CCAn_nnnn'nTGG
Cuts at: 735 1413 735
Size: 678 3968
BstYI r'GATC_y
Cuts at: 1 395 541 1091 1410 1671 2138 2161 2496
Size: 394 146 550 319 261 467 23 335
Cuts at: 2496 2742 3048 3060 3146 4198 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 550 467 449 394 335 319 306
261 246 146 86 23 12
BtgI C'CryG_G
Cuts at: 1108 1108
Size: 4646
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 392 627 2596 2777 2940 392
Size: 235 1969 181 163 2098
Fragments arranged by size:
2098 1969 235 181 163
BtsI GCAGTG_nn'
Cuts at: 571 626 1016 1160 1181 1557 1889 571
Size: 55 390 144 21 376 332 3328
Fragments arranged by size:
3328 390 376 332 144 55 21
DraI TTT'AAA
Cuts at: 1332 1645 1332
Size: 313 4333
DrdI GACnn_nn'nnGTC
Cuts at: 2352 3696 2352
Size: 1344 3302
EaeI y'GGCC_r
Cuts at: 546 806 1002 1275 2231 2405 2796 2823 4168
Size: 260 196 273 956 174 391 27 1345
Cuts at: 4168 546
Size: 1024
Fragments arranged by size:
1345 1024 956 391 273 260 196 174
27
EagI C'GGCC_G
Cuts at: 2231 2231
Size: 4646
EarI CTCTTCn'nnn_
Cuts at: 985 1343 2669 2879 985
Size: 358 1326 210 2752
Fragments arranged by size:
2752 1326 358 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2854 3596 3742 4456 2854
Size: 742 146 714 3044
Fragments arranged by size:
3044 742 714 146
EcoICRI GAG'CTC
Cuts at: 682 1658 682
Size: 976 3670
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 757 2471 2903 3256 3918 757
Size: 1714 432 353 662 1485
Fragments arranged by size:
1714 1485 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 520 1113 1785 520
Size: 593 672 3381
Fragments arranged by size:
3381 672 593
EcoRI G'AATT_C
Cuts at: 1650 1650
Size: 4646
EcoRV GAT'ATC
Cuts at: 480 480
Size: 4646
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1601 2665 1601
Size: 1064 3582
FspI TGC'GCA
Cuts at: 2427 2427
Size: 4646
HaeII r_GCGC'y
Cuts at: 2080 2328 3558 4329 2080
Size: 248 1230 771 2397
Fragments arranged by size:
2397 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 509 541 1296 1328 2488 2518 2520 2550 509
Size: 32 755 32 1160 30 2 30 2605
Fragments arranged by size:
2605 1160 755 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1473 1685 1473
Size: 212 4434
HindIII A'AGCT_T
Cuts at: 411 411
Size: 4646
KasI G'GCGC_C
Cuts at: 2076 2324 2076
Size: 248 4398
KpnI G_GTAC'C
Cuts at: 1666 1666
Size: 4646
MfeI C'AATT_G
Cuts at: 1259 1259
Size: 4646
MluI A'CGCG_T
Cuts at: 4079 4079
Size: 4646
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 288 567 610 709 887 1504 3405 3589 288
Size: 279 43 99 178 617 1901 184 1345
Fragments arranged by size:
1901 1345 617 279 184 178 99 43
MscI TGG'CCA
Cuts at: 548 808 1004 1277 2407 548
Size: 260 196 273 1130 2787
Fragments arranged by size:
2787 1130 273 260 196
MslI CAynn'nnrTG
Cuts at: 646 703 802 853 1529 2635 2762 646
Size: 57 99 51 676 1106 127 2530
Fragments arranged by size:
2530 1106 676 127 99 57 51
MspA1I CmG'CkG
Cuts at: 1000 1757 2071 2431 3215 3460 1000
Size: 757 314 360 784 245 2186
Fragments arranged by size:
2186 784 757 360 314 245
NaeI GCC'GGC
Cuts at: 2827 2827
Size: 4646
NarI GG'CG_CC
Cuts at: 2077 2325 2077
Size: 248 4398
NcoI C'CATG_G
Cuts at: 1108 1108
Size: 4646
NgoMIV G'CCGG_C
Cuts at: 2825 2825
Size: 4646
NspI r_CATG'y
Cuts at: 1164 1699 2199 3802 1164
Size: 535 500 1603 2008
Fragments arranged by size:
2008 1603 535 500
PciI A'CATG_T
Cuts at: 3798 3798
Size: 4646
PflMI CCAn_nnn'nTGG
Cuts at: 1486 1486
Size: 4646
PmeI GTTT'AAAC
Cuts at: 1645 1645
Size: 4646
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3092 3124 3092
Size: 32 4614
PpuMI rG'GwC_Cy
Cuts at: 520 1113 520
Size: 593 4053
PstI C_TGCA'G
Cuts at: 1693 2378 1693
Size: 685 3961
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4646
PvuII CAG'CTG
Cuts at: 1000 2071 2431 1000
Size: 1071 360 3215
Fragments arranged by size:
3215 1071 360
RsrII CG'GwC_CG
Cuts at: 2841 2841
Size: 4646
SacI G_AGCT'C
Cuts at: 684 1660 684
Size: 976 3670
SalI G'TCGA_C
Cuts at: 1683 1683
Size: 4646
SanDI GG'GwC_CC
Cuts at: 1113 1113
Size: 4646
SapI GCTCTTCn'nnn_
Cuts at: 2669 2879 2669
Size: 210 4436
SbfI CC_TGCA'GG
Cuts at: 1693 1693
Size: 4646
ScaI AGT'ACT
Cuts at: 404 4163 404
Size: 3759 887
SfcI C'TryA_G
Cuts at: 33 1599 1689 2374 3342 3533 33
Size: 1566 90 685 968 191 1146
Fragments arranged by size:
1566 1146 968 685 191 90
SfoI GGC'GCC
Cuts at: 2078 2326 2078
Size: 248 4398
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4646
SmaI CCC'GGG
Cuts at: 1668 1668
Size: 4646
SmlI C'TyrA_G
Cuts at: 1801 2716 3430 3692 1801
Size: 915 714 262 2755
Fragments arranged by size:
2755 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4646
SphI G_CATG'C
Cuts at: 1699 2199 1699
Size: 500 4146
StuI AGG'CCT
Cuts at: 581 1081 581
Size: 500 4146
StyI C'CwwG_G
Cuts at: 728 1108 1623 1780 2134 728
Size: 380 515 157 354 3240
Fragments arranged by size:
3240 515 380 354 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 445 2229 2362 2821 3030 445
Size: 1784 133 459 209 2061
Fragments arranged by size:
2061 1784 459 209 133
TatI w'GTAC_w
Cuts at: 402 1568 1588 4161 402
Size: 1166 20 2573 887
Fragments arranged by size:
2573 1166 887 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 401 433 851 883 1270 1302 4163 4195 401
Size: 32 418 32 387 32 2861 32 852
Fragments arranged by size:
2861 852 418 387 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2443 2443
Size: 4646
XbaI T'CTAG_A
Cuts at: 1677 1677
Size: 4646
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2141 2141
Size: 4646
XmaI C'CCGG_G
Cuts at: 1666 1666
Size: 4646
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI
BsiWI BsmBI BspEI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI FseI FspAI HpaI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI