Restriction map for
pF1KB5159
(Circular) (Six-base) MAPSORT of: pF1KB5159.seq Check: 8454 from: 1 to: 3701
pF1KB5159 3701 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 252 252
Size: 3701
AatII G_ACGT'C
Cuts at: 344 344
Size: 3701
AccI GT'mk_AC
Cuts at: 739 739
Size: 3701
Acc65I G'GTAC_C
Cuts at: 717 717
Size: 3701
AclI AA'CG_TT
Cuts at: 3541 3541
Size: 3701
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1526 1958 2311 2973 1526
Size: 432 353 662 2254
Fragments arranged by size:
2254 662 432 353
AfeI AGC'GCT
Cuts at: 652 652
Size: 3701
AflIII A'CryG_T
Cuts at: 1053 2853 3134 1053
Size: 1800 281 1620
Fragments arranged by size:
1800 1620 281
AlwNI CAG_nnn'CTG
Cuts at: 1006 2444 1006
Size: 1438 2263
ApaLI G'TGCA_C
Cuts at: 2539 2539
Size: 3701
ApoI r'AATT_y
Cuts at: 705 3054 3310 705
Size: 2349 256 1096
Fragments arranged by size:
2349 1096 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3701
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3701
AvaI C'yCGr_G
Cuts at: 199 394 409 577 721 199
Size: 195 15 168 144 3179
Fragments arranged by size:
3179 195 168 144 15
BamHI G'GATC_C
Cuts at: 726 726
Size: 3701
BanI G'GyrC_C
Cuts at: 717 1131 1379 1414 717
Size: 414 248 35 3004
Fragments arranged by size:
3004 414 248 35
BanII G_rGCy'C
Cuts at: 715 1745 715
Size: 1030 2671
BbeI G_GCGC'C
Cuts at: 1135 1383 1135
Size: 248 3453
BbsI GAAGACnn'nnnn_
Cuts at: 213 609 213
Size: 396 3305
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 568 602 568
Size: 34 3667
BciVI GTATCCnnnnn_n'
Cuts at: 1599 2655 3701 1599
Size: 1056 1046 1599
Fragments arranged by size:
1599 1056 1046
BclI T'GATC_A
Cuts at: 1221 1221
Size: 3701
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3701
BglII A'GATC_T
Cuts at: 1216 3253 1216
Size: 2037 1664
BlpI GC'TnA_GC
Cuts at: 813 813
Size: 3701
Bme1580I G_kGCm'C
Cuts at: 215 440 1326 1419 2543 215
Size: 225 886 93 1124 1373
Fragments arranged by size:
1373 1124 886 225 93
BmgBI CAC'GTC
Cuts at: 164 164
Size: 3701
BmrI ACTGGGnnnn_n'
Cuts at: 913 994 1329 3473 913
Size: 81 335 2144 1141
Fragments arranged by size:
2144 1141 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2068 2068
Size: 3701
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 403
Size: 3701
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 877 1756 2470 2768 877
Size: 879 714 298 1810
Fragments arranged by size:
1810 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3405 3405
Size: 3701
BsaAI yAC'GTr
Cuts at: 210 1054 210
Size: 844 2857
BsaBI GATnn'nnATC
Cuts at: 6 1215 3252 6
Size: 1209 2037 455
Fragments arranged by size:
2037 1209 455
BsaHI Gr'CG_yC
Cuts at: 341 1132 1380 2082 3578 341
Size: 791 248 702 1496 464
Fragments arranged by size:
1496 791 702 464 248
BsaWI w'CCGG_w
Cuts at: 890 1411 2089 2500 2647 2921 890
Size: 521 678 411 147 274 1670
Fragments arranged by size:
1670 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1126 1884 2549 2957 1126
Size: 758 665 408 1870
Fragments arranged by size:
1870 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 309 319 540 1243 1289 2519 104
Size: 205 10 221 703 46 1230 1286
Fragments arranged by size:
1286 1230 703 221 205 46 10
BsiHKAI G_wGCw'C
Cuts at: 586 715 1493 1683 2543 586
Size: 129 778 190 860 1744
Fragments arranged by size:
1744 860 778 190 129
BsmBI CGTCTCn'nnnn_
Cuts at: 616 616
Size: 3701
Bsp1286I G_dGCh'C
Cuts at: 215 440 586 715 1326 1419 1493 1683 1745
Size: 225 146 129 611 93 74 190 62
Cuts at: 1745 2543 215
Size: 798 1373
Fragments arranged by size:
1373 798 611 225 190 146 129 93
74 62
BspHI T'CATG_A
Cuts at: 2133 2133
Size: 3701
BspMI ACCTGCnnnn'nnnn_
Cuts at: 252 476 751 965 1267 1648 252
Size: 224 275 214 302 381 2305
Fragments arranged by size:
2305 381 302 275 224 214
BsrBI CCG'CTC
Cuts at: 50 321 1993 2047 3531 3696 50
Size: 271 1672 54 1484 165 55
Fragments arranged by size:
1672 1484 271 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1613 1613
Size: 3701
BsrFI r'CCGG_y
Cuts at: 1699 1880 1699
Size: 181 3520
BssHII G'CGCG_C
Cuts at: 633 633
Size: 3701
BssSI C'ACGA_G
Cuts at: 1972 2680 1972
Size: 708 2993
BstAPI GCAn_nnn'nTGC
Cuts at: 164 680 1078 164
Size: 516 398 2787
Fragments arranged by size:
2787 516 398
BstBI TT'CG_AA
Cuts at: 2062 2062
Size: 3701
BstXI CCAn_nnnn'nTGG
Cuts at: 447 447
Size: 3701
BstYI r'GATC_y
Cuts at: 1 726 1193 1216 1551 1797 2103 2115 2201
Size: 725 467 23 335 246 306 12 86
Cuts at: 2201 3253 1
Size: 1052 449
Fragments arranged by size:
1052 725 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 545 107
Size: 438 3263
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1651 1832 1995 1651
Size: 181 163 3357
Fragments arranged by size:
3357 181 163
BtsI GCAGTG_nn'
Cuts at: 944 944
Size: 3701
DraI TTT'AAA
Cuts at: 700 700
Size: 3701
DrdI GACnn_nn'nnGTC
Cuts at: 340 484 1407 2751 340
Size: 144 923 1344 1290
Fragments arranged by size:
1344 1290 923 144
EaeI y'GGCC_r
Cuts at: 113 236 316 452 537 548 626 1286 1460
Size: 123 80 136 85 11 78 660 174
Cuts at: 1460 1851 1878 3223 113
Size: 391 27 1345 591
Fragments arranged by size:
1345 660 591 391 174 136 123 85
80 78 27 11
EagI C'GGCC_G
Cuts at: 316 537 1286 316
Size: 221 749 2731
Fragments arranged by size:
2731 749 221
EarI CTCTTCn'nnn_
Cuts at: 1724 1934 1724
Size: 210 3491
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1909 2651 2797 3511 1909
Size: 742 146 714 2099
Fragments arranged by size:
2099 742 714 146
EcoICRI GAG'CTC
Cuts at: 713 713
Size: 3701
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 1526 1958 2311 2973 403
Size: 1123 432 353 662 1131
Fragments arranged by size:
1131 1123 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 405 840 405
Size: 435 3266
EcoRI G'AATT_C
Cuts at: 705 705
Size: 3701
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1720 1720
Size: 3701
FspI TGC'GCA
Cuts at: 472 526 556 1482 472
Size: 54 30 926 2691
Fragments arranged by size:
2691 926 54 30
FspAI rTGC'GCAy
Cuts at: 526 556 526
Size: 30 3671
HaeII r_GCGC'y
Cuts at: 277 654 1135 1383 2613 3384 277
Size: 377 481 248 1230 771 594
Fragments arranged by size:
1230 771 594 481 377 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1543 1573 1575 1605 1543
Size: 30 2 30 3639
Fragments arranged by size:
3639 30 30 2
HincII GTy'rAC
Cuts at: 740 740
Size: 3701
KasI G'GCGC_C
Cuts at: 1131 1379 1131
Size: 248 3453
KpnI G_GTAC'C
Cuts at: 721 721
Size: 3701
MluI A'CGCG_T
Cuts at: 3134 3134
Size: 3701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2460 2644 2460
Size: 184 3517
MscI TGG'CCA
Cuts at: 454 1462 454
Size: 1008 2693
MslI CAynn'nnrTG
Cuts at: 247 349 448 550 1690 1817 247
Size: 102 99 102 1140 127 2131
Fragments arranged by size:
2131 1140 127 102 102 99
MspA1I CmG'CkG
Cuts at: 812 1126 1486 2270 2515 812
Size: 314 360 784 245 1998
Fragments arranged by size:
1998 784 360 314 245
NaeI GCC'GGC
Cuts at: 1882 1882
Size: 3701
NarI GG'CG_CC
Cuts at: 1132 1380 1132
Size: 248 3453
NcoI C'CATG_G
Cuts at: 107 545 107
Size: 438 3263
NgoMIV G'CCGG_C
Cuts at: 1880 1880
Size: 3701
NotI GC'GGCC_GC
Cuts at: 316 316
Size: 3701
NspI r_CATG'y
Cuts at: 487 754 1254 2857 487
Size: 267 500 1603 1331
Fragments arranged by size:
1603 1331 500 267
PciI A'CATG_T
Cuts at: 2853 2853
Size: 3701
PmeI GTTT'AAAC
Cuts at: 700 700
Size: 3701
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2147 2179 2147
Size: 32 3669
PpuMI rG'GwC_Cy
Cuts at: 405 405
Size: 3701
PspXI vC'TCGA_Gb
Cuts at: 577 577
Size: 3701
PstI C_TGCA'G
Cuts at: 647 748 1433 647
Size: 101 685 2915
Fragments arranged by size:
2915 685 101
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3701
PvuII CAG'CTG
Cuts at: 1126 1486 1126
Size: 360 3341
RsrII CG'GwC_CG
Cuts at: 1896 1896
Size: 3701
SacI G_AGCT'C
Cuts at: 715 715
Size: 3701
SalI G'TCGA_C
Cuts at: 738 738
Size: 3701
SanDI GG'GwC_CC
Cuts at: 405 405
Size: 3701
SapI GCTCTTCn'nnn_
Cuts at: 1724 1934 1724
Size: 210 3491
SbfI CC_TGCA'GG
Cuts at: 748 748
Size: 3701
ScaI AGT'ACT
Cuts at: 3218 3218
Size: 3701
SfcI C'TryA_G
Cuts at: 33 643 744 1429 2397 2588 33
Size: 610 101 685 968 191 1146
Fragments arranged by size:
1146 968 685 610 191 101
SfoI GGC'GCC
Cuts at: 1133 1381 1133
Size: 248 3453
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3701
SmaI CCC'GGG
Cuts at: 723 723
Size: 3701
SmlI C'TyrA_G
Cuts at: 577 856 1771 2485 2747 577
Size: 279 915 714 262 1531
Fragments arranged by size:
1531 915 714 279 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3701
SphI G_CATG'C
Cuts at: 754 1254 754
Size: 500 3201
StyI C'CwwG_G
Cuts at: 107 545 596 835 1189 107
Size: 438 51 239 354 2619
Fragments arranged by size:
2619 438 354 239 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1284 1417 1876 2085 1284
Size: 133 459 209 2900
Fragments arranged by size:
2900 459 209 133
TatI w'GTAC_w
Cuts at: 3216 3216
Size: 3701
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3218 3250 3218
Size: 32 3669
Tth111I GACn'n_nGTC
Cuts at: 1498 1498
Size: 3701
XbaI T'CTAG_A
Cuts at: 732 732
Size: 3701
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 447 1196 447
Size: 749 2952
XhoI C'TCGA_G
Cuts at: 577 577
Size: 3701
XmaI C'CCGG_G
Cuts at: 721 721
Size: 3701
XmnI GAAnn'nnTTC
Cuts at: 421 421
Size: 3701
ZraI GAC'GTC
Cuts at: 342 342
Size: 3701
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PciI PmeI PpiI PpuMI
PspXI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BmtI Bpu10I BsaXI BseRI
BsgI BsiWI BsmI BspEI BsrGI BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI HindIII HpaI
MfeI NdeI NheI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspOMI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI