Restriction map for
pF1KB5164
(Circular) (Six-base) MAPSORT of: pF1KB5164.seq Check: 7935 from: 1 to: 4394
pF1KB5164 4394 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1432 1432
Size: 4394
Acc65I G'GTAC_C
Cuts at: 1410 1410
Size: 4394
AclI AA'CG_TT
Cuts at: 4234 4234
Size: 4394
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 2219 2651 3004 3666 703
Size: 1516 432 353 662 1431
Fragments arranged by size:
1516 1431 662 432 353
AflIII A'CryG_T
Cuts at: 1746 3546 3827 1746
Size: 1800 281 2313
Fragments arranged by size:
2313 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 618 850 963 1699 3137 618
Size: 232 113 736 1438 1875
Fragments arranged by size:
1875 1438 736 232 113
ApaI G_GGCC'C
Cuts at: 668 957 1079 1378 668
Size: 289 122 299 3684
Fragments arranged by size:
3684 299 289 122
ApaLI G'TGCA_C
Cuts at: 3232 3232
Size: 4394
ApoI r'AATT_y
Cuts at: 342 1398 3747 4003 342
Size: 1056 2349 256 733
Fragments arranged by size:
2349 1056 733 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4394
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
AvaI C'yCGr_G
Cuts at: 304 1244 1414 304
Size: 940 170 3284
Fragments arranged by size:
3284 940 170
BamHI G'GATC_C
Cuts at: 128 1419 128
Size: 1291 3103
BanI G'GyrC_C
Cuts at: 696 1255 1410 1824 2072 2107 696
Size: 559 155 414 248 35 2983
Fragments arranged by size:
2983 559 414 248 155 35
BanII G_rGCy'C
Cuts at: 605 628 668 957 1079 1378 1408 2438 605
Size: 23 40 289 122 299 30 1030 2561
Fragments arranged by size:
2561 1030 299 289 122 40 30 23
BbeI G_GCGC'C
Cuts at: 1828 2076 1828
Size: 248 4146
BbsI GAAGACnn'nnnn_
Cuts at: 524 744 524
Size: 220 4174
BbvCI CC'TCA_GC
Cuts at: 611 611
Size: 4394
BciVI GTATCCnnnnn_n'
Cuts at: 2292 3348 4394 2292
Size: 1056 1046 2292
Fragments arranged by size:
2292 1056 1046
BclI T'GATC_A
Cuts at: 1914 1914
Size: 4394
BglI GCCn_nnn'nGGC
Cuts at: 779 828 779
Size: 49 4345
BglII A'GATC_T
Cuts at: 1089 1909 3946 1089
Size: 820 2037 1537
Fragments arranged by size:
2037 1537 820
BlpI GC'TnA_GC
Cuts at: 901 1506 901
Size: 605 3789
Bme1580I G_kGCm'C
Cuts at: 668 957 971 1079 1214 1378 2019 2112 3236
Size: 289 14 108 135 164 641 93 1124
Cuts at: 3236 668
Size: 1826
Fragments arranged by size:
1826 1124 641 289 164 135 108 93
14
BmrI ACTGGGnnnn_n'
Cuts at: 547 993 1606 1687 2022 4166 547
Size: 446 613 81 335 2144 775
Fragments arranged by size:
2144 775 613 446 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2761 2761
Size: 4394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 1288 190
Size: 1098 3296
Bpu10I CC'TnA_GC
Cuts at: 599 611 599
Size: 12 4382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1570 2449 3163 3461 1570
Size: 879 714 298 2503
Fragments arranged by size:
2503 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4098 4098
Size: 4394
BsaAI yAC'GTr
Cuts at: 888 1217 1747 888
Size: 329 530 3535
Fragments arranged by size:
3535 530 329
BsaBI GATnn'nnATC
Cuts at: 6 1908 3945 6
Size: 1902 2037 455
Fragments arranged by size:
2037 1902 455
BsaHI Gr'CG_yC
Cuts at: 1825 2073 2775 4271 1825
Size: 248 702 1496 1948
Fragments arranged by size:
1948 1496 702 248
BsaWI w'CCGG_w
Cuts at: 392 477 1583 2104 2782 3193 3340 3614 392
Size: 85 1106 521 678 411 147 274 1172
Fragments arranged by size:
1172 1106 678 521 411 274 147 85
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1294 1324 1294
Size: 30 4364
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1012 1251 1012
Size: 239 4155
BseYI C'CCAG_C
Cuts at: 461 628 956 963 1819 2577 3242 3650 461
Size: 167 328 7 856 758 665 408 1205
Fragments arranged by size:
1205 856 758 665 408 328 167 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1270 1270
Size: 4394
BsiEI CG_ry'CG
Cuts at: 104 1936 1982 3212 104
Size: 1832 46 1230 1286
Fragments arranged by size:
1832 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1408 2186 2376 3236 1408
Size: 778 190 860 2566
Fragments arranged by size:
2566 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 605 628 668 957 971 1079 1214 1378 1408
Size: 23 40 289 14 108 135 164 30
Cuts at: 1408 2019 2112 2186 2376 2438 3236 605
Size: 611 93 74 190 62 798 1763
Fragments arranged by size:
1763 798 611 289 190 164 135 108
93 74 62 40 30 23 14
BspEI T'CCGG_A
Cuts at: 477 477
Size: 4394
BspHI T'CATG_A
Cuts at: 2826 2826
Size: 4394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 332 1292 1349 1444 1658 1960 2341 332
Size: 960 57 95 214 302 381 2385
Fragments arranged by size:
2385 960 381 302 214 95 57
BsrBI CCG'CTC
Cuts at: 50 350 2686 2740 4224 4389 50
Size: 300 2336 54 1484 165 55
Fragments arranged by size:
2336 1484 300 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2306 2306
Size: 4394
BsrFI r'CCGG_y
Cuts at: 2392 2573 2392
Size: 181 4213
BssSI C'ACGA_G
Cuts at: 1192 2665 3373 1192
Size: 1473 708 2213
Fragments arranged by size:
2213 1473 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1771 1771
Size: 4394
BstBI TT'CG_AA
Cuts at: 2755 2755
Size: 4394
BstXI CCAn_nnnn'nTGG
Cuts at: 1378 1378
Size: 4394
BstYI r'GATC_y
Cuts at: 1 128 567 1089 1419 1886 1909 2244 2490
Size: 127 439 522 330 467 23 335 246
Cuts at: 2490 2796 2808 2894 3946 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 522 467 449 439 335 330 306
246 127 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1222 1222
Size: 4394
BtgI C'CryG_G
Cuts at: 107 124 1177 107
Size: 17 1053 3324
Fragments arranged by size:
3324 1053 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2344 2525 2688 2344
Size: 181 163 4050
Fragments arranged by size:
4050 181 163
BtsI GCAGTG_nn'
Cuts at: 838 949 969 995 1637 838
Size: 111 20 26 642 3595
Fragments arranged by size:
3595 642 111 26 20
DraI TTT'AAA
Cuts at: 1393 1393
Size: 4394
DrdI GACnn_nn'nnGTC
Cuts at: 2100 3444 2100
Size: 1344 3050
EaeI y'GGCC_r
Cuts at: 829 1979 2153 2544 2571 3916 829
Size: 1150 174 391 27 1345 1307
Fragments arranged by size:
1345 1307 1150 391 174 27
EagI C'GGCC_G
Cuts at: 1979 1979
Size: 4394
EarI CTCTTCn'nnn_
Cuts at: 331 701 2417 2627 331
Size: 370 1716 210 2098
Fragments arranged by size:
2098 1716 370 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2602 3344 3490 4204 2602
Size: 742 146 714 2792
Fragments arranged by size:
2792 742 714 146
EcoICRI GAG'CTC
Cuts at: 1406 1406
Size: 4394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 703 1288 2219 2651 3004 3666 190
Size: 513 585 931 432 353 662 918
Fragments arranged by size:
931 918 662 585 513 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 235 336 235
Size: 101 4293
EcoO109I rG'GnC_Cy
Cuts at: 402 574 664 665 780 1038 1075 1076 1226
Size: 172 90 1 115 258 37 1 150
Cuts at: 1226 1374 1375 1533 402
Size: 148 1 158 3263
Fragments arranged by size:
3263 258 172 158 150 148 115 90
37 1 1 1
EcoRI G'AATT_C
Cuts at: 1398 1398
Size: 4394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4394
FspI TGC'GCA
Cuts at: 2175 2175
Size: 4394
HaeII r_GCGC'y
Cuts at: 1828 2076 3306 4077 1828
Size: 248 1230 771 2145
Fragments arranged by size:
2145 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 818 850 911 943 2236 2266 2268 2298 818
Size: 32 61 32 1293 30 2 30 2914
Fragments arranged by size:
2914 1293 61 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1433 1433
Size: 4394
KasI G'GCGC_C
Cuts at: 1824 2072 1824
Size: 248 4146
KpnI G_GTAC'C
Cuts at: 1414 1414
Size: 4394
MluI A'CGCG_T
Cuts at: 3827 3827
Size: 4394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1319 1346 3153 3337 1319
Size: 27 1807 184 2376
Fragments arranged by size:
2376 1807 184 27
MscI TGG'CCA
Cuts at: 831 2155 831
Size: 1324 3070
MslI CAynn'nnrTG
Cuts at: 2383 2510 2383
Size: 127 4267
MspA1I CmG'CkG
Cuts at: 153 960 1016 1505 1819 2179 2963 3208 153
Size: 807 56 489 314 360 784 245 1339
Fragments arranged by size:
1339 807 784 489 360 314 245 56
NaeI GCC'GGC
Cuts at: 2575 2575
Size: 4394
NarI GG'CG_CC
Cuts at: 1825 2073 1825
Size: 248 4146
NcoI C'CATG_G
Cuts at: 107 107
Size: 4394
NdeI CA'TA_TG
Cuts at: 297 297
Size: 4394
NgoMIV G'CCGG_C
Cuts at: 2573 2573
Size: 4394
NspI r_CATG'y
Cuts at: 858 898 1447 1947 3550 858
Size: 40 549 500 1603 1702
Fragments arranged by size:
1702 1603 549 500 40
PasI CC'CwG_GG
Cuts at: 1378 1378
Size: 4394
PciI A'CATG_T
Cuts at: 3546 3546
Size: 4394
PflMI CCAn_nnn'nTGG
Cuts at: 850 963 850
Size: 113 4281
PfoI T'CCnGG_A
Cuts at: 570 570
Size: 4394
PmeI GTTT'AAAC
Cuts at: 1393 1393
Size: 4394
PmlI CAC'GTG
Cuts at: 1217 1217
Size: 4394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 700 732 2840 2872 700
Size: 32 2108 32 2222
Fragments arranged by size:
2222 2108 32 32
PpuMI rG'GwC_Cy
Cuts at: 402 574 1038 402
Size: 172 464 3758
Fragments arranged by size:
3758 464 172
PspOMI G'GGCC_C
Cuts at: 664 953 1075 1374 664
Size: 289 122 299 3684
Fragments arranged by size:
3684 299 289 122
PstI C_TGCA'G
Cuts at: 1346 1441 2126 1346
Size: 95 685 3614
Fragments arranged by size:
3614 685 95
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4394
PvuII CAG'CTG
Cuts at: 153 1819 2179 153
Size: 1666 360 2368
Fragments arranged by size:
2368 1666 360
RsrII CG'GwC_CG
Cuts at: 2589 2589
Size: 4394
SacI G_AGCT'C
Cuts at: 1408 1408
Size: 4394
SalI G'TCGA_C
Cuts at: 1431 1431
Size: 4394
SanDI GG'GwC_CC
Cuts at: 574 574
Size: 4394
SapI GCTCTTCn'nnn_
Cuts at: 2417 2627 2417
Size: 210 4184
SbfI CC_TGCA'GG
Cuts at: 1346 1441 1346
Size: 95 4299
ScaI AGT'ACT
Cuts at: 501 3911 501
Size: 3410 984
SfcI C'TryA_G
Cuts at: 33 590 1342 1437 2122 3090 3281 33
Size: 557 752 95 685 968 191 1146
Fragments arranged by size:
1146 968 752 685 557 191 95
SfoI GGC'GCC
Cuts at: 1826 2074 1826
Size: 248 4146
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
SmaI CCC'GGG
Cuts at: 306 1246 1416 306
Size: 940 170 3284
Fragments arranged by size:
3284 940 170
SmlI C'TyrA_G
Cuts at: 1549 2464 3178 3440 1549
Size: 915 714 262 2503
Fragments arranged by size:
2503 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4394
SphI G_CATG'C
Cuts at: 1447 1947 1447
Size: 500 3894
StuI AGG'CCT
Cuts at: 274 1336 274
Size: 1062 3332
StyI C'CwwG_G
Cuts at: 107 217 1043 1229 1528 1882 107
Size: 110 826 186 299 354 2619
Fragments arranged by size:
2619 826 354 299 186 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 174 901 1319 1977 2110 2569 2778 174
Size: 727 418 658 133 459 209 1790
Fragments arranged by size:
1790 727 658 459 418 209 133
TatI w'GTAC_w
Cuts at: 499 1328 3909 499
Size: 829 2581 984
Fragments arranged by size:
2581 984 829
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1162 1194 1294 1326 3911 3943 1162
Size: 32 100 32 2585 32 1613
Fragments arranged by size:
2585 1613 100 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2191 2191
Size: 4394
XbaI T'CTAG_A
Cuts at: 1425 1425
Size: 4394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1889 1889
Size: 4394
XmaI C'CCGG_G
Cuts at: 304 1244 1414 304
Size: 940 170 3284
Fragments arranged by size:
3284 940 170
XmnI GAAnn'nnTTC
Cuts at: 521 521
Size: 4394
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NheI NotI NruI NsiI PacI PshAI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI