Restriction map for
pF1KB5166
(Circular) (Six-base) MAPSORT of: pF1KB5166.seq Check: 1311 from: 1 to: 3587
pF1KB5166 3587 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 625 625
Size: 3587
Acc65I G'GTAC_C
Cuts at: 603 603
Size: 3587
AclI AA'CG_TT
Cuts at: 3427 3427
Size: 3587
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 128 256 1412 1844 2591 2859 128
Size: 128 1156 432 747 268 856
Fragments arranged by size:
1156 856 747 432 268 128
AflIII A'CryG_T
Cuts at: 939 2043 3020 939
Size: 1104 977 1506
Fragments arranged by size:
1506 1104 977
AgeI A'CCGG_T
Cuts at: 1975 1975
Size: 3587
AlwNI CAG_nnn'CTG
Cuts at: 892 2459 892
Size: 1567 2020
ApaLI G'TGCA_C
Cuts at: 2357 2357
Size: 3587
ApoI r'AATT_y
Cuts at: 591 2940 3196 591
Size: 2349 256 982
Fragments arranged by size:
2349 982 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3587
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3587
AvaI C'yCGr_G
Cuts at: 607 607
Size: 3587
BamHI G'GATC_C
Cuts at: 612 612
Size: 3587
BanI G'GyrC_C
Cuts at: 314 603 1017 1265 1300 314
Size: 289 414 248 35 2601
Fragments arranged by size:
2601 414 289 248 35
BanII G_rGCy'C
Cuts at: 601 1631 601
Size: 1030 2557
BbeI G_GCGC'C
Cuts at: 318 1021 1269 318
Size: 703 248 2636
Fragments arranged by size:
2636 703 248
BciVI GTATCCnnnnn_n'
Cuts at: 1485 2246 3587 1485
Size: 761 1341 1485
Fragments arranged by size:
1485 1341 761
BclI T'GATC_A
Cuts at: 1107 1107
Size: 3587
BglII A'GATC_T
Cuts at: 1102 3139 1102
Size: 2037 1550
BlpI GC'TnA_GC
Cuts at: 699 699
Size: 3587
Bme1580I G_kGCm'C
Cuts at: 1212 1305 2361 1212
Size: 93 1056 2438
Fragments arranged by size:
2438 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 340 799 880 1215 3359 340
Size: 459 81 335 2144 568
Fragments arranged by size:
2144 568 459 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 376 1954 376
Size: 1578 2009
Bpu10I CC'TnA_GC
Cuts at: 271 271
Size: 3587
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 763 1642 2134 2432 763
Size: 879 492 298 1918
Fragments arranged by size:
1918 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 388 3291 388
Size: 2903 684
BsaAI yAC'GTr
Cuts at: 940 940
Size: 3587
BsaBI GATnn'nnATC
Cuts at: 6 1101 3138 6
Size: 1095 2037 455
Fragments arranged by size:
2037 1095 455
BsaHI Gr'CG_yC
Cuts at: 315 1018 1266 1968 3464 315
Size: 703 248 702 1496 438
Fragments arranged by size:
1496 703 702 438 248
BsaWI w'CCGG_w
Cuts at: 776 1297 1975 2249 2396 2807 776
Size: 521 678 274 147 411 1556
Fragments arranged by size:
1556 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 505 505
Size: 3587
BseYI C'CCAG_C
Cuts at: 297 1012 1770 2347 2843 297
Size: 715 758 577 496 1041
Fragments arranged by size:
1041 758 715 577 496
BsiEI CG_ry'CG
Cuts at: 104 1129 1175 2383 104
Size: 1025 46 1208 1308
Fragments arranged by size:
1308 1208 1025 46
BsiHKAI G_wGCw'C
Cuts at: 601 1379 1569 2361 601
Size: 778 190 792 1827
Fragments arranged by size:
1827 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 601 1212 1305 1379 1569 1631 2361 601
Size: 611 93 74 190 62 730 1827
Fragments arranged by size:
1827 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2807 2807
Size: 3587
BspHI T'CATG_A
Cuts at: 2763 2763
Size: 3587
BspMI ACCTGCnnnn'nnnn_
Cuts at: 637 851 1153 1534 637
Size: 214 302 381 2690
Fragments arranged by size:
2690 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1879 1933 3417 3582 50
Size: 1829 54 1484 165 55
Fragments arranged by size:
1829 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1499 1499
Size: 3587
BsrFI r'CCGG_y
Cuts at: 293 1585 1766 1975 293
Size: 1292 181 209 1905
Fragments arranged by size:
1905 1292 209 181
BssSI C'ACGA_G
Cuts at: 259 1858 2216 259
Size: 1599 358 1630
Fragments arranged by size:
1630 1599 358
BstAPI GCAn_nnn'nTGC
Cuts at: 964 964
Size: 3587
BstBI TT'CG_AA
Cuts at: 1948 1948
Size: 3587
BstYI r'GATC_y
Cuts at: 1 173 612 1079 1102 1437 1683 2695 2781
Size: 172 439 467 23 335 246 1012 86
Cuts at: 2781 2793 3139 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 439 346 335 246 172
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1537 1718 1881 1537
Size: 181 163 3243
Fragments arranged by size:
3243 181 163
BtsI GCAGTG_nn'
Cuts at: 830 830
Size: 3587
DraI TTT'AAA
Cuts at: 586 586
Size: 3587
DrdI GACnn_nn'nnGTC
Cuts at: 1293 2151 1293
Size: 858 2729
EaeI y'GGCC_r
Cuts at: 116 1172 1346 1737 1764 3109 116
Size: 1056 174 391 27 1345 594
Fragments arranged by size:
1345 1056 594 391 174 27
EagI C'GGCC_G
Cuts at: 1172 1172
Size: 3587
EarI CTCTTCn'nnn_
Cuts at: 124 1610 1820 124
Size: 1486 210 1891
Fragments arranged by size:
1891 1486 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1795 2105 2251 3397 1795
Size: 310 146 1146 1985
Fragments arranged by size:
1985 1146 310 146
EcoICRI GAG'CTC
Cuts at: 599 599
Size: 3587
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 128 256 1412 1844 2591 2859 128
Size: 128 1156 432 747 268 856
Fragments arranged by size:
1156 856 747 432 268 128
EcoO109I rG'GnC_Cy
Cuts at: 726 726
Size: 3587
EcoRI G'AATT_C
Cuts at: 591 591
Size: 3587
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1606 1606
Size: 3587
FspI TGC'GCA
Cuts at: 390 1368 390
Size: 978 2609
HaeII r_GCGC'y
Cuts at: 318 1021 1269 2291 3270 318
Size: 703 248 1022 979 635
Fragments arranged by size:
1022 979 703 635 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 342 374 500 532 1429 1459 1461 1491 342
Size: 32 126 32 897 30 2 30 2438
Fragments arranged by size:
2438 897 126 32 32 30 30 2
HincII GTy'rAC
Cuts at: 626 626
Size: 3587
KasI G'GCGC_C
Cuts at: 314 1017 1265 314
Size: 703 248 2636
Fragments arranged by size:
2636 703 248
KpnI G_GTAC'C
Cuts at: 607 607
Size: 3587
MluI A'CGCG_T
Cuts at: 3020 3020
Size: 3587
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2258 2442 2258
Size: 184 3403
MscI TGG'CCA
Cuts at: 1348 1348
Size: 3587
MslI CAynn'nnrTG
Cuts at: 494 1576 1703 494
Size: 1082 127 2378
Fragments arranged by size:
2378 1082 127
MspA1I CmG'CkG
Cuts at: 698 1012 1372 2385 2630 698
Size: 314 360 1013 245 1655
Fragments arranged by size:
1655 1013 360 314 245
NaeI GCC'GGC
Cuts at: 295 1768 295
Size: 1473 2114
NarI GG'CG_CC
Cuts at: 315 1018 1266 315
Size: 703 248 2636
Fragments arranged by size:
2636 703 248
NgoMIV G'CCGG_C
Cuts at: 293 1766 293
Size: 1473 2114
NspI r_CATG'y
Cuts at: 197 640 1140 2047 197
Size: 443 500 907 1737
Fragments arranged by size:
1737 907 500 443
PciI A'CATG_T
Cuts at: 2043 2043
Size: 3587
PmeI GTTT'AAAC
Cuts at: 586 586
Size: 3587
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2726 2758 2726
Size: 32 3555
PstI C_TGCA'G
Cuts at: 173 634 1319 173
Size: 461 685 2441
Fragments arranged by size:
2441 685 461
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3587
PvuII CAG'CTG
Cuts at: 1012 1372 1012
Size: 360 3227
RsrII CG'GwC_CG
Cuts at: 1782 1782
Size: 3587
SacI G_AGCT'C
Cuts at: 601 601
Size: 3587
SalI G'TCGA_C
Cuts at: 624 624
Size: 3587
SapI GCTCTTCn'nnn_
Cuts at: 124 1610 1820 124
Size: 1486 210 1891
Fragments arranged by size:
1891 1486 210
SbfI CC_TGCA'GG
Cuts at: 634 634
Size: 3587
ScaI AGT'ACT
Cuts at: 253 3104 253
Size: 2851 736
SfcI C'TryA_G
Cuts at: 33 169 630 1315 2308 2499 33
Size: 136 461 685 993 191 1121
Fragments arranged by size:
1121 993 685 461 191 136
SfoI GGC'GCC
Cuts at: 316 1019 1267 316
Size: 703 248 2636
Fragments arranged by size:
2636 703 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3587
SmaI CCC'GGG
Cuts at: 609 609
Size: 3587
SmlI C'TyrA_G
Cuts at: 742 1657 2149 2411 742
Size: 915 492 262 1918
Fragments arranged by size:
1918 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3587
SphI G_CATG'C
Cuts at: 640 1140 640
Size: 500 3087
StyI C'CwwG_G
Cuts at: 721 1075 721
Size: 354 3233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 289 1170 1303 1762 1971 289
Size: 881 133 459 209 1905
Fragments arranged by size:
1905 881 459 209 133
TatI w'GTAC_w
Cuts at: 251 3102 251
Size: 2851 736
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3104 3136 3104
Size: 32 3555
Tth111I GACn'n_nGTC
Cuts at: 1384 1384
Size: 3587
XbaI T'CTAG_A
Cuts at: 618 618
Size: 3587
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 214 1082 214
Size: 868 2719
XmaI C'CCGG_G
Cuts at: 607 607
Size: 3587
XmnI GAAnn'nnTTC
Cuts at: 490 490
Size: 3587
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI BsaXI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI