Restriction map for
pF1KB5167
(Circular) (Six-base) MAPSORT of: pF1KB5167.seq Check: 3813 from: 1 to: 4673
pF1KB5167 4673 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1296 1296
Size: 4673
AccI GT'mk_AC
Cuts at: 404 1628 1711 404
Size: 1224 83 3366
Fragments arranged by size:
3366 1224 83
Acc65I G'GTAC_C
Cuts at: 1689 1689
Size: 4673
AclI AA'CG_TT
Cuts at: 4513 4513
Size: 4673
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 940 1168 2498 2930 3677 3945 940
Size: 228 1330 432 747 268 1668
Fragments arranged by size:
1668 1330 747 432 268 228
AfeI AGC'GCT
Cuts at: 190 221 190
Size: 31 4642
AflIII A'CryG_T
Cuts at: 728 908 2025 3129 4106 728
Size: 180 1117 1104 977 1295
Fragments arranged by size:
1295 1117 1104 977 180
AgeI A'CCGG_T
Cuts at: 3061 3061
Size: 4673
AlwNI CAG_nnn'CTG
Cuts at: 1035 1978 3545 1035
Size: 943 1567 2163
Fragments arranged by size:
2163 1567 943
ApaI G_GGCC'C
Cuts at: 791 791
Size: 4673
ApaLI G'TGCA_C
Cuts at: 3443 3443
Size: 4673
ApoI r'AATT_y
Cuts at: 1677 4026 4282 1677
Size: 2349 256 2068
Fragments arranged by size:
2349 2068 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4673
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
AvaI C'yCGr_G
Cuts at: 1693 1693
Size: 4673
BamHI G'GATC_C
Cuts at: 1698 1698
Size: 4673
BanI G'GyrC_C
Cuts at: 1237 1285 1612 1689 2103 2351 2386 1237
Size: 48 327 77 414 248 35 3524
Fragments arranged by size:
3524 414 327 248 77 48 35
BanII G_rGCy'C
Cuts at: 398 791 1202 1687 2717 398
Size: 393 411 485 1030 2354
Fragments arranged by size:
2354 1030 485 411 393
BbeI G_GCGC'C
Cuts at: 1616 2107 2355 1616
Size: 491 248 3934
Fragments arranged by size:
3934 491 248
BbsI GAAGACnn'nnnn_
Cuts at: 160 215 947 1361 1643 160
Size: 55 732 414 282 3190
Fragments arranged by size:
3190 732 414 282 55
BbvCI CC'TCA_GC
Cuts at: 1486 1486
Size: 4673
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 715 749 715
Size: 34 4639
BciVI GTATCCnnnnn_n'
Cuts at: 2571 3332 4673 2571
Size: 761 1341 2571
Fragments arranged by size:
2571 1341 761
BclI T'GATC_A
Cuts at: 2193 2193
Size: 4673
BglI GCCn_nnn'nGGC
Cuts at: 1082 1417 1082
Size: 335 4338
BglII A'GATC_T
Cuts at: 2188 4225 2188
Size: 2037 2636
BlpI GC'TnA_GC
Cuts at: 1785 1785
Size: 4673
Bme1580I G_kGCm'C
Cuts at: 283 791 895 2298 2391 3447 283
Size: 508 104 1403 93 1056 1509
Fragments arranged by size:
1509 1403 1056 508 104 93
BmgBI CAC'GTC
Cuts at: 729 882 729
Size: 153 4520
BmrI ACTGGGnnnn_n'
Cuts at: 440 1885 1966 2301 4445 440
Size: 1445 81 335 2144 668
Fragments arranged by size:
2144 1445 668 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 549 1599 3040 549
Size: 1050 1441 2182
Fragments arranged by size:
2182 1441 1050
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1591 1591
Size: 4673
Bpu10I CC'TnA_GC
Cuts at: 1486 1486
Size: 4673
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 476 1384 1849 2728 3220 3518 476
Size: 908 465 879 492 298 1631
Fragments arranged by size:
1631 908 879 492 465 298
BsaI GGTCTCn'nnnn_
Cuts at: 4377 4377
Size: 4673
BsaAI yAC'GTr
Cuts at: 2026 2026
Size: 4673
BsaBI GATnn'nnATC
Cuts at: 6 2187 4224 6
Size: 2181 2037 455
Fragments arranged by size:
2181 2037 455
BsaHI Gr'CG_yC
Cuts at: 1115 1613 2104 2352 3054 4550 1115
Size: 498 491 248 702 1496 1238
Fragments arranged by size:
1496 1238 702 498 491 248
BsaWI w'CCGG_w
Cuts at: 1862 2383 3061 3335 3482 3893 1862
Size: 521 678 274 147 411 2642
Fragments arranged by size:
2642 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1063 1093 1063
Size: 30 4643
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 217 1081 1084 1210 1381 1588 1615 1663 217
Size: 864 3 126 171 207 27 48 3227
Fragments arranged by size:
3227 864 207 171 126 48 27 3
BseYI C'CCAG_C
Cuts at: 2098 2856 3433 3929 2098
Size: 758 577 496 2842
Fragments arranged by size:
2842 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1273 1480 1495 1273
Size: 207 15 4451
Fragments arranged by size:
4451 207 15
BsiEI CG_ry'CG
Cuts at: 104 161 1473 2215 2261 3469 104
Size: 57 1312 742 46 1208 1308
Fragments arranged by size:
1312 1308 1208 742 57 46
BsiHKAI G_wGCw'C
Cuts at: 398 471 1313 1439 1687 2465 2655 3447 398
Size: 73 842 126 248 778 190 792 1624
Fragments arranged by size:
1624 842 792 778 248 190 126 73
BsmBI CGTCTCn'nnnn_
Cuts at: 1570 1570
Size: 4673
Bsp1286I G_dGCh'C
Cuts at: 283 398 471 791 895 1202 1313 1439 1687
Size: 115 73 320 104 307 111 126 248
Cuts at: 1687 2298 2391 2465 2655 2717 3447 283
Size: 611 93 74 190 62 730 1509
Fragments arranged by size:
1509 730 611 320 307 248 190 126
115 111 104 93 74 73 62
BspEI T'CCGG_A
Cuts at: 3893 3893
Size: 4673
BspHI T'CATG_A
Cuts at: 3849 3849
Size: 4673
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1296 1502 1517 1723 1937 2239 2620 1296
Size: 206 15 206 214 302 381 3349
Fragments arranged by size:
3349 381 302 214 206 206 15
BsrBI CCG'CTC
Cuts at: 50 1655 2965 3019 4503 4668 50
Size: 1605 1310 54 1484 165 55
Fragments arranged by size:
1605 1484 1310 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2585 2585
Size: 4673
BsrFI r'CCGG_y
Cuts at: 1218 1372 1465 2671 2852 3061 1218
Size: 154 93 1206 181 209 2830
Fragments arranged by size:
2830 1206 209 181 154 93
BssSI C'ACGA_G
Cuts at: 2944 3302 2944
Size: 358 4315
BstAPI GCAn_nnn'nTGC
Cuts at: 1434 2050 1434
Size: 616 4057
BstBI TT'CG_AA
Cuts at: 3034 3034
Size: 4673
BstEII G'GTnAC_C
Cuts at: 1293 1479 1293
Size: 186 4487
BstYI r'GATC_y
Cuts at: 1 567 1698 2165 2188 2523 2769 3781 3867
Size: 566 1131 467 23 335 246 1012 86
Cuts at: 3867 3879 4225 1
Size: 12 346 449
Fragments arranged by size:
1131 1012 566 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 141 991 1007 1013 1289 107
Size: 34 850 16 6 276 3491
Fragments arranged by size:
3491 850 276 34 16 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2623 2804 2967 2623
Size: 181 163 4329
Fragments arranged by size:
4329 181 163
BtsI GCAGTG_nn'
Cuts at: 1497 1916 1497
Size: 419 4254
DraI TTT'AAA
Cuts at: 1672 1672
Size: 4673
DrdI GACnn_nn'nnGTC
Cuts at: 1477 2379 3237 1477
Size: 902 858 2913
Fragments arranged by size:
2913 902 858
EaeI y'GGCC_r
Cuts at: 158 369 587 1010 1274 2258 2432 2823 2850
Size: 211 218 423 264 984 174 391 27
Cuts at: 2850 4195 158
Size: 1345 636
Fragments arranged by size:
1345 984 636 423 391 264 218 211
174 27
EagI C'GGCC_G
Cuts at: 158 2258 158
Size: 2100 2573
EarI CTCTTCn'nnn_
Cuts at: 658 930 1058 1583 2696 2906 658
Size: 272 128 525 1113 210 2425
Fragments arranged by size:
2425 1113 525 272 210 128
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 515 2881 3191 3337 4483 515
Size: 2366 310 146 1146 705
Fragments arranged by size:
2366 1146 705 310 146
EcoICRI GAG'CTC
Cuts at: 396 1685 396
Size: 1289 3384
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 940 1168 1591 2498 2930 3677 3945 940
Size: 228 423 907 432 747 268 1668
Fragments arranged by size:
1668 907 747 432 423 268 228
EcoNI CCTnn'n_nnAGG
Cuts at: 294 1366 294
Size: 1072 3601
EcoO109I rG'GnC_Cy
Cuts at: 576 787 1153 1246 1812 576
Size: 211 366 93 566 3437
Fragments arranged by size:
3437 566 366 211 93
EcoRI G'AATT_C
Cuts at: 1677 1677
Size: 4673
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1378 2692 1378
Size: 1314 3359
FspI TGC'GCA
Cuts at: 2454 2454
Size: 4673
HaeII r_GCGC'y
Cuts at: 192 223 465 1616 2107 2355 3377 4356 192
Size: 31 242 1151 491 248 1022 979 509
Fragments arranged by size:
1151 1022 979 509 491 248 242 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 630 659 662 691 1063 1095 2515 2545 2547
Size: 29 3 29 372 32 1420 30 2
Cuts at: 2547 2577 630
Size: 30 2726
Fragments arranged by size:
2726 1420 372 32 30 30 29 29
3 2
HincII GTy'rAC
Cuts at: 438 1629 1712 438
Size: 1191 83 3399
Fragments arranged by size:
3399 1191 83
KasI G'GCGC_C
Cuts at: 1612 2103 2351 1612
Size: 491 248 3934
Fragments arranged by size:
3934 491 248
KpnI G_GTAC'C
Cuts at: 1693 1693
Size: 4673
MfeI C'AATT_G
Cuts at: 670 670
Size: 4673
MluI A'CGCG_T
Cuts at: 4106 4106
Size: 4673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 616 1430 3344 3528 616
Size: 814 1914 184 1761
Fragments arranged by size:
1914 1761 814 184
MscI TGG'CCA
Cuts at: 589 1012 2434 589
Size: 423 1422 2828
Fragments arranged by size:
2828 1422 423
MslI CAynn'nnrTG
Cuts at: 2662 2789 2662
Size: 127 4546
MspA1I CmG'CkG
Cuts at: 143 507 1146 1257 1643 1784 2098 2458 3471
Size: 364 639 111 386 141 314 360 1013
Cuts at: 3471 3716 143
Size: 245 1100
Fragments arranged by size:
1100 1013 639 386 364 360 314 245
141 111
NaeI GCC'GGC
Cuts at: 1220 1374 1467 2854 1220
Size: 154 93 1387 3039
Fragments arranged by size:
3039 1387 154 93
NarI GG'CG_CC
Cuts at: 1613 2104 2352 1613
Size: 491 248 3934
Fragments arranged by size:
3934 491 248
NcoI C'CATG_G
Cuts at: 107 1013 107
Size: 906 3767
NgoMIV G'CCGG_C
Cuts at: 1218 1372 1465 2852 1218
Size: 154 93 1387 3039
Fragments arranged by size:
3039 1387 154 93
NspI r_CATG'y
Cuts at: 912 1458 1726 2226 3133 912
Size: 546 268 500 907 2452
Fragments arranged by size:
2452 907 546 500 268
PasI CC'CwG_GG
Cuts at: 828 1242 828
Size: 414 4259
PciI A'CATG_T
Cuts at: 908 3129 908
Size: 2221 2452
PflMI CCAn_nnn'nTGG
Cuts at: 480 599 997 480
Size: 119 398 4156
Fragments arranged by size:
4156 398 119
PmeI GTTT'AAAC
Cuts at: 1672 1672
Size: 4673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 3812
Size: 32 4641
PpuMI rG'GwC_Cy
Cuts at: 576 1153 576
Size: 577 4096
PshAI GACnn'nnGTC
Cuts at: 1158 1158
Size: 4673
PspOMI G'GGCC_C
Cuts at: 787 787
Size: 4673
PstI C_TGCA'G
Cuts at: 1720 2405 1720
Size: 685 3988
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4673
PvuII CAG'CTG
Cuts at: 507 1146 1257 2098 2458 507
Size: 639 111 841 360 2722
Fragments arranged by size:
2722 841 639 360 111
RsrII CG'GwC_CG
Cuts at: 2868 2868
Size: 4673
SacI G_AGCT'C
Cuts at: 398 1687 398
Size: 1289 3384
SacII CC_GC'GG
Cuts at: 144 144
Size: 4673
SalI G'TCGA_C
Cuts at: 1627 1710 1627
Size: 83 4590
SanDI GG'GwC_CC
Cuts at: 1153 1153
Size: 4673
SapI GCTCTTCn'nnn_
Cuts at: 2696 2906 2696
Size: 210 4463
SbfI CC_TGCA'GG
Cuts at: 1720 1720
Size: 4673
ScaI AGT'ACT
Cuts at: 4190 4190
Size: 4673
SfcI C'TryA_G
Cuts at: 33 1716 2401 3394 3585 33
Size: 1683 685 993 191 1121
Fragments arranged by size:
1683 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1614 2105 2353 1614
Size: 491 248 3934
Fragments arranged by size:
3934 491 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
SmaI CCC'GGG
Cuts at: 1695 1695
Size: 4673
SmlI C'TyrA_G
Cuts at: 491 1363 1828 2743 3235 3497 491
Size: 872 465 915 492 262 1667
Fragments arranged by size:
1667 915 872 492 465 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4673
SphI G_CATG'C
Cuts at: 1726 2226 1726
Size: 500 4173
SspI AAT'ATT
Cuts at: 361 361
Size: 4673
StuI AGG'CCT
Cuts at: 1534 1534
Size: 4673
StyI C'CwwG_G
Cuts at: 107 295 1013 1535 1807 2161 107
Size: 188 718 522 272 354 2619
Fragments arranged by size:
2619 718 522 354 272 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 810 1255 2256 2389 2848 3057 810
Size: 445 1001 133 459 209 2426
Fragments arranged by size:
2426 1001 459 445 209 133
TatI w'GTAC_w
Cuts at: 700 1103 4188 700
Size: 403 3085 1185
Fragments arranged by size:
3085 1185 403
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 262 294 4190 4222 262
Size: 32 3896 32 713
Fragments arranged by size:
3896 713 32 32
Tth111I GACn'n_nGTC
Cuts at: 2470 2470
Size: 4673
XbaI T'CTAG_A
Cuts at: 1704 1704
Size: 4673
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1413 2168 1413
Size: 755 3918
XmaI C'CCGG_G
Cuts at: 1693 1693
Size: 4673
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BfrBI BmtI BsiWI BsmI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PfoI PmlI PsiI PspXI PsrI SexAI SfiI SgrAI
SnaBI SrfI SwaI XhoI XmnI ZraI