Restriction map for
pF1KB5177
(Circular) (Six-base) MAPSORT of: pF1KB5177.seq Check: 474 from: 1 to: 3764
pF1KB5177 3764 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 271 802 271
Size: 531 3233
Acc65I G'GTAC_C
Cuts at: 780 780
Size: 3764
AclI AA'CG_TT
Cuts at: 603 3604 603
Size: 3001 763
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 264 310 338 357 1589 2021 2768 3036 264
Size: 46 28 19 1232 432 747 268 992
Fragments arranged by size:
1232 992 747 432 268 46 28 19
AflIII A'CryG_T
Cuts at: 1116 2220 3197 1116
Size: 1104 977 1683
Fragments arranged by size:
1683 1104 977
AgeI A'CCGG_T
Cuts at: 2152 2152
Size: 3764
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 172 172
Size: 3764
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 351 383 351
Size: 32 3732
AlwNI CAG_nnn'CTG
Cuts at: 1069 2636 1069
Size: 1567 2197
ApaLI G'TGCA_C
Cuts at: 2534 2534
Size: 3764
ApoI r'AATT_y
Cuts at: 141 623 717 768 3117 3373 141
Size: 482 94 51 2349 256 532
Fragments arranged by size:
2349 532 482 256 94 51
AseI AT'TA_AT
Cuts at: 20 353 20
Size: 333 3431
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
AvaI C'yCGr_G
Cuts at: 117 784 117
Size: 667 3097
BamHI G'GATC_C
Cuts at: 789 789
Size: 3764
BanI G'GyrC_C
Cuts at: 156 780 1194 1442 1477 156
Size: 624 414 248 35 2443
Fragments arranged by size:
2443 624 414 248 35
BanII G_rGCy'C
Cuts at: 778 1808 778
Size: 1030 2734
BbeI G_GCGC'C
Cuts at: 1198 1446 1198
Size: 248 3516
BbvCI CC'TCA_GC
Cuts at: 252 252
Size: 3764
BciVI GTATCCnnnnn_n'
Cuts at: 115 211 1662 2423 3764 115
Size: 96 1451 761 1341 115
Fragments arranged by size:
1451 1341 761 115 96
BclI T'GATC_A
Cuts at: 501 1284 501
Size: 783 2981
BfrBI ATG'CAT
Cuts at: 445 445
Size: 3764
BglII A'GATC_T
Cuts at: 1279 3316 1279
Size: 2037 1727
BlpI GC'TnA_GC
Cuts at: 876 876
Size: 3764
Bme1580I G_kGCm'C
Cuts at: 748 1389 1482 2538 748
Size: 641 93 1056 1974
Fragments arranged by size:
1974 1056 641 93
BmrI ACTGGGnnnn_n'
Cuts at: 976 1057 1392 3536 976
Size: 81 335 2144 1204
Fragments arranged by size:
2144 1204 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 3764
Bpu10I CC'TnA_GC
Cuts at: 252 252
Size: 3764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 940 1819 2311 2609 940
Size: 879 492 298 2095
Fragments arranged by size:
2095 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 686 3468 686
Size: 2782 982
BsaAI yAC'GTr
Cuts at: 1117 1117
Size: 3764
BsaBI GATnn'nnATC
Cuts at: 6 416 1278 3315 6
Size: 410 862 2037 455
Fragments arranged by size:
2037 862 455 410
BsaHI Gr'CG_yC
Cuts at: 1195 1443 2145 3641 1195
Size: 248 702 1496 1318
Fragments arranged by size:
1496 1318 702 248
BsaWI w'CCGG_w
Cuts at: 953 1474 2152 2426 2573 2984 953
Size: 521 678 274 147 411 1733
Fragments arranged by size:
1733 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 351 381 351
Size: 30 3734
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 143 199 143
Size: 56 3708
BseYI C'CCAG_C
Cuts at: 198 1189 1947 2524 3020 198
Size: 991 758 577 496 942
Fragments arranged by size:
991 942 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1306 1352 2560 104
Size: 1202 46 1208 1308
Fragments arranged by size:
1308 1208 1202 46
BsiHKAI G_wGCw'C
Cuts at: 778 1556 1746 2538 778
Size: 778 190 792 2004
Fragments arranged by size:
2004 792 778 190
BsmI GAATG_Cn'
Cuts at: 149 170 445 149
Size: 21 275 3468
Fragments arranged by size:
3468 275 21
Bsp1286I G_dGCh'C
Cuts at: 748 778 1389 1482 1556 1746 1808 2538 748
Size: 30 611 93 74 190 62 730 1974
Fragments arranged by size:
1974 730 611 190 93 74 62 30
BspEI T'CCGG_A
Cuts at: 2984 2984
Size: 3764
BspHI T'CATG_A
Cuts at: 491 531 2940 491
Size: 40 2409 1315
Fragments arranged by size:
2409 1315 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 394 814 1028 1330 1711 394
Size: 420 214 302 381 2447
Fragments arranged by size:
2447 420 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2056 2110 3594 3759 50
Size: 2006 54 1484 165 55
Fragments arranged by size:
2006 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1676 1676
Size: 3764
BsrFI r'CCGG_y
Cuts at: 1762 1943 2152 1762
Size: 181 209 3374
Fragments arranged by size:
3374 209 181
BssSI C'ACGA_G
Cuts at: 2035 2393 2035
Size: 358 3406
BstAPI GCAn_nnn'nTGC
Cuts at: 1141 1141
Size: 3764
BstBI TT'CG_AA
Cuts at: 2125 2125
Size: 3764
BstYI r'GATC_y
Cuts at: 1 789 1256 1279 1614 1860 2872 2958 2970
Size: 788 467 23 335 246 1012 86 12
Cuts at: 2970 3316 1
Size: 346 449
Fragments arranged by size:
1012 788 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 272 272
Size: 3764
BtgI C'CryG_G
Cuts at: 107 107
Size: 3764
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1714 1895 2058 1714
Size: 181 163 3420
Fragments arranged by size:
3420 181 163
BtsI GCAGTG_nn'
Cuts at: 1007 1007
Size: 3764
DraI TTT'AAA
Cuts at: 763 763
Size: 3764
DrdI GACnn_nn'nnGTC
Cuts at: 1470 2328 1470
Size: 858 2906
EaeI y'GGCC_r
Cuts at: 1349 1523 1914 1941 3286 1349
Size: 174 391 27 1345 1827
Fragments arranged by size:
1827 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1349 1349
Size: 3764
EarI CTCTTCn'nnn_
Cuts at: 1787 1997 1787
Size: 210 3554
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1972 2282 2428 3574 1972
Size: 310 146 1146 2162
Fragments arranged by size:
2162 1146 310 146
EcoICRI GAG'CTC
Cuts at: 776 776
Size: 3764
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 264 310 338 357 1589 2021 2768 3036 264
Size: 46 28 19 1232 432 747 268 992
Fragments arranged by size:
1232 992 747 432 268 46 28 19
EcoO109I rG'GnC_Cy
Cuts at: 903 903
Size: 3764
EcoRI G'AATT_C
Cuts at: 768 768
Size: 3764
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 504 1783 504
Size: 1279 2485
FspI TGC'GCA
Cuts at: 1545 1545
Size: 3764
HaeII r_GCGC'y
Cuts at: 1198 1446 2468 3447 1198
Size: 248 1022 979 1515
Fragments arranged by size:
1515 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 205 237 486 518 1606 1636 1638 1668 205
Size: 32 249 32 1088 30 2 30 2301
Fragments arranged by size:
2301 1088 249 32 32 30 30 2
HincII GTy'rAC
Cuts at: 803 803
Size: 3764
HindIII A'AGCT_T
Cuts at: 658 658
Size: 3764
KasI G'GCGC_C
Cuts at: 1194 1442 1194
Size: 248 3516
KpnI G_GTAC'C
Cuts at: 784 784
Size: 3764
MfeI C'AATT_G
Cuts at: 755 755
Size: 3764
MluI A'CGCG_T
Cuts at: 3197 3197
Size: 3764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 389 2435 2619 389
Size: 2046 184 1534
Fragments arranged by size:
2046 1534 184
MscI TGG'CCA
Cuts at: 1525 1525
Size: 3764
MslI CAynn'nnrTG
Cuts at: 154 1753 1880 154
Size: 1599 127 2038
Fragments arranged by size:
2038 1599 127
MspA1I CmG'CkG
Cuts at: 256 875 1189 1549 2562 2807 256
Size: 619 314 360 1013 245 1213
Fragments arranged by size:
1213 1013 619 360 314 245
NaeI GCC'GGC
Cuts at: 1945 1945
Size: 3764
NarI GG'CG_CC
Cuts at: 1195 1443 1195
Size: 248 3516
NcoI C'CATG_G
Cuts at: 107 107
Size: 3764
NgoMIV G'CCGG_C
Cuts at: 1943 1943
Size: 3764
NsiI A_TGCA'T
Cuts at: 447 447
Size: 3764
NspI r_CATG'y
Cuts at: 817 1317 2224 817
Size: 500 907 2357
Fragments arranged by size:
2357 907 500
PciI A'CATG_T
Cuts at: 2220 2220
Size: 3764
PmeI GTTT'AAAC
Cuts at: 763 763
Size: 3764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 351 383 2903 2935 351
Size: 32 2520 32 1180
Fragments arranged by size:
2520 1180 32 32
PstI C_TGCA'G
Cuts at: 391 811 1496 391
Size: 420 685 2659
Fragments arranged by size:
2659 685 420
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3764
PvuII CAG'CTG
Cuts at: 256 1189 1549 256
Size: 933 360 2471
Fragments arranged by size:
2471 933 360
RsrII CG'GwC_CG
Cuts at: 1959 1959
Size: 3764
SacI G_AGCT'C
Cuts at: 778 778
Size: 3764
SalI G'TCGA_C
Cuts at: 801 801
Size: 3764
SapI GCTCTTCn'nnn_
Cuts at: 1787 1997 1787
Size: 210 3554
SbfI CC_TGCA'GG
Cuts at: 811 811
Size: 3764
ScaI AGT'ACT
Cuts at: 3281 3281
Size: 3764
SfcI C'TryA_G
Cuts at: 33 387 807 1492 2485 2676 33
Size: 354 420 685 993 191 1121
Fragments arranged by size:
1121 993 685 420 354 191
SfoI GGC'GCC
Cuts at: 1196 1444 1196
Size: 248 3516
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
SmaI CCC'GGG
Cuts at: 119 786 119
Size: 667 3097
SmlI C'TyrA_G
Cuts at: 919 1834 2326 2588 919
Size: 915 492 262 2095
Fragments arranged by size:
2095 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3764
SphI G_CATG'C
Cuts at: 817 1317 817
Size: 500 3264
StyI C'CwwG_G
Cuts at: 107 898 1252 107
Size: 791 354 2619
Fragments arranged by size:
2619 791 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1347 1480 1939 2148 1347
Size: 133 459 209 2963
Fragments arranged by size:
2963 459 209 133
TatI w'GTAC_w
Cuts at: 3279 3279
Size: 3764
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 167 199 3281 3313 167
Size: 32 3082 32 618
Fragments arranged by size:
3082 618 32 32
Tth111I GACn'n_nGTC
Cuts at: 1561 1561
Size: 3764
XbaI T'CTAG_A
Cuts at: 795 795
Size: 3764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 555 1259 555
Size: 704 3060
XmaI C'CCGG_G
Cuts at: 117 784 117
Size: 667 3097
XmnI GAAnn'nnTTC
Cuts at: 323 323
Size: 3764
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BstZ17I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
AvrII BaeI BbsI BcgI BglI BmgBI BmtI BpmI
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
NdeI NheI NotI NruI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI