Restriction map for
pF1KB5178
(Circular) (Six-base) MAPSORT of: pF1KB5178.seq Check: 3743 from: 1 to: 4568
pF1KB5178 4568 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 193 193
Size: 4568
AccI GT'mk_AC
Cuts at: 1432 1606 1432
Size: 174 4394
Acc65I G'GTAC_C
Cuts at: 1584 1584
Size: 4568
AclI AA'CG_TT
Cuts at: 4408 4408
Size: 4568
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 2393 2825 3572 3840 784
Size: 1609 432 747 268 1512
Fragments arranged by size:
1609 1512 747 432 268
AflIII A'CryG_T
Cuts at: 1920 3024 4001 1920
Size: 1104 977 2487
Fragments arranged by size:
2487 1104 977
AgeI A'CCGG_T
Cuts at: 2956 2956
Size: 4568
AleI CACnn'nnGTG
Cuts at: 730 1027 1073 730
Size: 297 46 4225
Fragments arranged by size:
4225 297 46
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 607 639 607
Size: 32 4536
AlwNI CAG_nnn'CTG
Cuts at: 675 717 786 1033 1290 1873 3440 675
Size: 42 69 247 257 583 1567 1803
Fragments arranged by size:
1803 1567 583 257 247 69 42
ApaLI G'TGCA_C
Cuts at: 3338 3338
Size: 4568
ApoI r'AATT_y
Cuts at: 1572 3921 4177 1572
Size: 2349 256 1963
Fragments arranged by size:
2349 1963 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4568
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4568
AvaI C'yCGr_G
Cuts at: 1588 1588
Size: 4568
BamHI G'GATC_C
Cuts at: 1593 1593
Size: 4568
BanI G'GyrC_C
Cuts at: 529 1057 1584 1998 2246 2281 529
Size: 528 527 414 248 35 2816
Fragments arranged by size:
2816 528 527 414 248 35
BanII G_rGCy'C
Cuts at: 998 1424 1582 2612 998
Size: 426 158 1030 2954
Fragments arranged by size:
2954 1030 426 158
BbeI G_GCGC'C
Cuts at: 2002 2250 2002
Size: 248 4320
BbsI GAAGACnn'nnnn_
Cuts at: 407 898 407
Size: 491 4077
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4534
BciVI GTATCCnnnnn_n'
Cuts at: 2466 3227 4568 2466
Size: 761 1341 2466
Fragments arranged by size:
2466 1341 761
BclI T'GATC_A
Cuts at: 1503 2088 1503
Size: 585 3983
BglI GCCn_nnn'nGGC
Cuts at: 661 661
Size: 4568
BglII A'GATC_T
Cuts at: 2083 4120 2083
Size: 2037 2531
BlpI GC'TnA_GC
Cuts at: 1304 1552 1680 1304
Size: 248 128 4192
Fragments arranged by size:
4192 248 128
Bme1580I G_kGCm'C
Cuts at: 1060 1112 2193 2286 3342 1060
Size: 52 1081 93 1056 2286
Fragments arranged by size:
2286 1081 1056 93 52
BmrI ACTGGGnnnn_n'
Cuts at: 120 1780 1861 2196 4340 120
Size: 1660 81 335 2144 348
Fragments arranged by size:
2144 1660 348 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2935 2935
Size: 4568
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1092 1092
Size: 4568
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1088 1744 2623 3115 3413 1088
Size: 656 879 492 298 2243
Fragments arranged by size:
2243 879 656 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1186 4272 1186
Size: 3086 1482
BsaAI yAC'GTr
Cuts at: 1921 1921
Size: 4568
BsaBI GATnn'nnATC
Cuts at: 6 2082 4119 6
Size: 2076 2037 455
Fragments arranged by size:
2076 2037 455
BsaHI Gr'CG_yC
Cuts at: 1999 2247 2949 4445 1999
Size: 248 702 1496 2122
Fragments arranged by size:
2122 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1757 2278 2956 3230 3377 3788 1757
Size: 521 678 274 147 411 2537
Fragments arranged by size:
2537 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 607 637 905 935 607
Size: 30 268 30 4240
Fragments arranged by size:
4240 268 30 30
BseYI C'CCAG_C
Cuts at: 710 1993 2751 3328 3824 710
Size: 1283 758 577 496 1454
Fragments arranged by size:
1454 1283 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 603 742 799 1306 1507 603
Size: 139 57 507 201 3664
Fragments arranged by size:
3664 507 201 139 57
BsiEI CG_ry'CG
Cuts at: 104 2110 2156 3364 104
Size: 2006 46 1208 1308
Fragments arranged by size:
2006 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 123 1558 1582 2360 2550 3342 123
Size: 1435 24 778 190 792 1349
Fragments arranged by size:
1435 1349 792 778 190 24
Bsp1286I G_dGCh'C
Cuts at: 123 998 1060 1112 1424 1558 1582 2193 2286
Size: 875 62 52 312 134 24 611 93
Cuts at: 2286 2360 2550 2612 3342 123
Size: 74 190 62 730 1349
Fragments arranged by size:
1349 875 730 611 312 190 134 93
74 62 62 52 24
BspEI T'CCGG_A
Cuts at: 3788 3788
Size: 4568
BspHI T'CATG_A
Cuts at: 3744 3744
Size: 4568
BspMI ACCTGCnnnn'nnnn_
Cuts at: 193 321 1289 1298 1346 1618 1832 2134 2515
Size: 128 968 9 48 272 214 302 381
Cuts at: 2515 193
Size: 2246
Fragments arranged by size:
2246 968 381 302 272 214 128 48
9
BsrBI CCG'CTC
Cuts at: 50 2860 2914 4398 4563 50
Size: 2810 54 1484 165 55
Fragments arranged by size:
2810 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 425 1359 2480 425
Size: 934 1121 2513
Fragments arranged by size:
2513 1121 934
BsrFI r'CCGG_y
Cuts at: 145 2566 2747 2956 145
Size: 2421 181 209 1757
Fragments arranged by size:
2421 1757 209 181
BsrGI T'GTAC_A
Cuts at: 215 666 215
Size: 451 4117
BssSI C'ACGA_G
Cuts at: 2839 3197 2839
Size: 358 4210
BstAPI GCAn_nnn'nTGC
Cuts at: 1290 1945 1290
Size: 655 3913
BstBI TT'CG_AA
Cuts at: 2929 2929
Size: 4568
BstEII G'GTnAC_C
Cuts at: 205 1467 205
Size: 1262 3306
BstXI CCAn_nnnn'nTGG
Cuts at: 444 732 444
Size: 288 4280
BstYI r'GATC_y
Cuts at: 1 1270 1593 2060 2083 2418 2664 3676 3762
Size: 1269 323 467 23 335 246 1012 86
Cuts at: 3762 3774 4120 1
Size: 12 346 449
Fragments arranged by size:
1269 1012 467 449 346 335 323 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 157 1454 157
Size: 1297 3271
BtgI C'CryG_G
Cuts at: 492 725 492
Size: 233 4335
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2518 2699 2862 2518
Size: 181 163 4224
Fragments arranged by size:
4224 181 163
BtsI GCAGTG_nn'
Cuts at: 1514 1811 1514
Size: 297 4271
DraI TTT'AAA
Cuts at: 1567 1567
Size: 4568
DrdI GACnn_nn'nnGTC
Cuts at: 2274 3132 2274
Size: 858 3710
EaeI y'GGCC_r
Cuts at: 434 989 1541 2153 2327 2718 2745 4090 434
Size: 555 552 612 174 391 27 1345 912
Fragments arranged by size:
1345 912 612 555 552 391 174 27
EagI C'GGCC_G
Cuts at: 2153 2153
Size: 4568
EarI CTCTTCn'nnn_
Cuts at: 2591 2801 2591
Size: 210 4358
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2776 3086 3232 4378 2776
Size: 310 146 1146 2966
Fragments arranged by size:
2966 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1580 1580
Size: 4568
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 1092 2393 2825 3572 3840 784
Size: 308 1301 432 747 268 1512
Fragments arranged by size:
1512 1301 747 432 308 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1168 1168
Size: 4568
EcoO109I rG'GnC_Cy
Cuts at: 354 645 672 783 1287 1707 354
Size: 291 27 111 504 420 3215
Fragments arranged by size:
3215 504 420 291 111 27
EcoRI G'AATT_C
Cuts at: 1572 1572
Size: 4568
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2587 2587
Size: 4568
FspI TGC'GCA
Cuts at: 638 2349 638
Size: 1711 2857
FspAI rTGC'GCAy
Cuts at: 638 638
Size: 4568
HaeII r_GCGC'y
Cuts at: 1127 1514 2002 2250 3272 4251 1127
Size: 387 488 248 1022 979 1444
Fragments arranged by size:
1444 1022 979 488 387 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1078 1110 1131 1163 2410 2440 2442 2472 1078
Size: 32 21 32 1247 30 2 30 3174
Fragments arranged by size:
3174 1247 32 32 30 30 21 2
HincII GTy'rAC
Cuts at: 1607 1607
Size: 4568
KasI G'GCGC_C
Cuts at: 1998 2246 1998
Size: 248 4320
KpnI G_GTAC'C
Cuts at: 1588 1588
Size: 4568
MluI A'CGCG_T
Cuts at: 4001 4001
Size: 4568
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3239 3423 3239
Size: 184 4384
MscI TGG'CCA
Cuts at: 436 1543 2329 436
Size: 1107 786 2675
Fragments arranged by size:
2675 1107 786
MslI CAynn'nnrTG
Cuts at: 730 1027 1073 1366 2557 2684 730
Size: 297 46 293 1191 127 2614
Fragments arranged by size:
2614 1191 297 293 127 46
MspA1I CmG'CkG
Cuts at: 267 369 571 714 1551 1679 1993 2353 3366
Size: 102 202 143 837 128 314 360 1013
Cuts at: 3366 3611 267
Size: 245 1224
Fragments arranged by size:
1224 1013 837 360 314 245 202 143
128 102
NaeI GCC'GGC
Cuts at: 2749 2749
Size: 4568
NarI GG'CG_CC
Cuts at: 1999 2247 1999
Size: 248 4320
NcoI C'CATG_G
Cuts at: 492 492
Size: 4568
NgoMIV G'CCGG_C
Cuts at: 2747 2747
Size: 4568
NspI r_CATG'y
Cuts at: 468 1621 2121 3028 468
Size: 1153 500 907 2008
Fragments arranged by size:
2008 1153 907 500
PasI CC'CwG_GG
Cuts at: 687 687
Size: 4568
PciI A'CATG_T
Cuts at: 3024 3024
Size: 4568
PfoI T'CCnGG_A
Cuts at: 1082 1082
Size: 4568
PmeI GTTT'AAAC
Cuts at: 1567 1567
Size: 4568
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 607 639 3707 3739 607
Size: 32 3068 32 1436
Fragments arranged by size:
3068 1436 32 32
PpuMI rG'GwC_Cy
Cuts at: 645 1287 645
Size: 642 3926
PstI C_TGCA'G
Cuts at: 204 662 1286 1615 2300 204
Size: 458 624 329 685 2472
Fragments arranged by size:
2472 685 624 458 329
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4568
PvuII CAG'CTG
Cuts at: 267 369 571 714 1993 2353 267
Size: 102 202 143 1279 360 2482
Fragments arranged by size:
2482 1279 360 202 143 102
RsrII CG'GwC_CG
Cuts at: 2763 2763
Size: 4568
SacI G_AGCT'C
Cuts at: 1582 1582
Size: 4568
SalI G'TCGA_C
Cuts at: 1605 1605
Size: 4568
SapI GCTCTTCn'nnn_
Cuts at: 2591 2801 2591
Size: 210 4358
SbfI CC_TGCA'GG
Cuts at: 662 1286 1615 662
Size: 624 329 3615
Fragments arranged by size:
3615 624 329
ScaI AGT'ACT
Cuts at: 4085 4085
Size: 4568
SfcI C'TryA_G
Cuts at: 33 200 358 580 658 1282 1611 2296 3289
Size: 167 158 222 78 624 329 685 993
Cuts at: 3289 3480 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 624 329 222 191 167
158 78
SfoI GGC'GCC
Cuts at: 2000 2248 2000
Size: 248 4320
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4568
SmaI CCC'GGG
Cuts at: 1590 1590
Size: 4568
SmlI C'TyrA_G
Cuts at: 1103 1723 2638 3130 3392 1103
Size: 620 915 492 262 2279
Fragments arranged by size:
2279 915 620 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4568
SphI G_CATG'C
Cuts at: 468 1621 2121 468
Size: 1153 500 2915
Fragments arranged by size:
2915 1153 500
StuI AGG'CCT
Cuts at: 755 755
Size: 4568
StyI C'CwwG_G
Cuts at: 492 557 601 960 1702 2056 492
Size: 65 44 359 742 354 3004
Fragments arranged by size:
3004 742 359 354 65 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 583 999 2151 2284 2743 2952 583
Size: 416 1152 133 459 209 2199
Fragments arranged by size:
2199 1152 459 416 209 133
TatI w'GTAC_w
Cuts at: 215 666 4083 215
Size: 451 3417 700
Fragments arranged by size:
3417 700 451
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 905 937 4085 4117 905
Size: 32 3148 32 1356
Fragments arranged by size:
3148 1356 32 32
Tth111I GACn'n_nGTC
Cuts at: 2365 2365
Size: 4568
XbaI T'CTAG_A
Cuts at: 1599 1599
Size: 4568
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1254 2063 1254
Size: 809 3759
XmaI C'CCGG_G
Cuts at: 1588 1588
Size: 4568
XmnI GAAnn'nnTTC
Cuts at: 976 976
Size: 4568
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI ApaI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BseRI BsiWI
BsmI BsmBI BssHII BstZ17I ClaI CspCI DraIII EcoRV
FseI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI