Restriction map for
pF1KB5179
(Circular) (Six-base) MAPSORT of: pF1KB5179.seq Check: 4271 from: 1 to: 4298
pF1KB5179 4298 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 977 1336 977
Size: 359 3939
Acc65I G'GTAC_C
Cuts at: 1114 1314 1114
Size: 200 4098
AclI AA'CG_TT
Cuts at: 4138 4138
Size: 4298
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 1225 2123 2555 3302 3570 229
Size: 996 898 432 747 268 957
Fragments arranged by size:
996 957 898 747 432 268
AflIII A'CryG_T
Cuts at: 1650 2754 3731 1650
Size: 1104 977 2217
Fragments arranged by size:
2217 1104 977
AgeI A'CCGG_T
Cuts at: 2686 2686
Size: 4298
AlwNI CAG_nnn'CTG
Cuts at: 1603 3170 1603
Size: 1567 2731
ApaLI G'TGCA_C
Cuts at: 3068 3068
Size: 4298
ApoI r'AATT_y
Cuts at: 1161 1302 3651 3907 1161
Size: 141 2349 256 1552
Fragments arranged by size:
2349 1552 256 141
AseI AT'TA_AT
Cuts at: 20 20
Size: 4298
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4298
AvaI C'yCGr_G
Cuts at: 1318 1318
Size: 4298
BamHI G'GATC_C
Cuts at: 252 1323 252
Size: 1071 3227
BanI G'GyrC_C
Cuts at: 946 1114 1314 1728 1976 2011 946
Size: 168 200 414 248 35 3233
Fragments arranged by size:
3233 414 248 200 168 35
BanII G_rGCy'C
Cuts at: 1312 2342 1312
Size: 1030 3268
BbeI G_GCGC'C
Cuts at: 1732 1980 1732
Size: 248 4050
BbsI GAAGACnn'nnnn_
Cuts at: 497 497
Size: 4298
BciVI GTATCCnnnnn_n'
Cuts at: 816 2196 2957 4298 816
Size: 1380 761 1341 816
Fragments arranged by size:
1380 1341 816 761
BclI T'GATC_A
Cuts at: 1818 1818
Size: 4298
BglI GCCn_nnn'nGGC
Cuts at: 598 617 598
Size: 19 4279
BglII A'GATC_T
Cuts at: 1813 3850 1813
Size: 2037 2261
BlpI GC'TnA_GC
Cuts at: 1410 1410
Size: 4298
Bme1580I G_kGCm'C
Cuts at: 157 1277 1923 2016 3072 157
Size: 1120 646 93 1056 1383
Fragments arranged by size:
1383 1120 1056 646 93
BmrI ACTGGGnnnn_n'
Cuts at: 1510 1591 1926 4070 1510
Size: 81 335 2144 1738
Fragments arranged by size:
2144 1738 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2665 2665
Size: 4298
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 524 524
Size: 4298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 638 1474 2353 2845 3143 446
Size: 192 836 879 492 298 1601
Fragments arranged by size:
1601 879 836 492 298 192
BsaI GGTCTCn'nnnn_
Cuts at: 500 1065 4002 500
Size: 565 2937 796
Fragments arranged by size:
2937 796 565
BsaAI yAC'GTr
Cuts at: 1651 1651
Size: 4298
BsaBI GATnn'nnATC
Cuts at: 6 1199 1812 3849 6
Size: 1193 613 2037 455
Fragments arranged by size:
2037 1193 613 455
BsaHI Gr'CG_yC
Cuts at: 1729 1977 2679 4175 1729
Size: 248 702 1496 1852
Fragments arranged by size:
1852 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1487 2008 2686 2960 3107 3518 1487
Size: 521 678 274 147 411 2267
Fragments arranged by size:
2267 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 430 460 430
Size: 30 4268
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 562 956 163
Size: 399 394 3505
Fragments arranged by size:
3505 399 394
BseYI C'CCAG_C
Cuts at: 310 1723 2481 3058 3554 310
Size: 1413 758 577 496 1054
Fragments arranged by size:
1413 1054 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 685 685
Size: 4298
BsiEI CG_ry'CG
Cuts at: 104 182 981 1840 1886 3094 104
Size: 78 799 859 46 1208 1308
Fragments arranged by size:
1308 1208 859 799 78 46
BsiHKAI G_wGCw'C
Cuts at: 936 1312 2090 2280 3072 936
Size: 376 778 190 792 2162
Fragments arranged by size:
2162 792 778 376 190
Bsp1286I G_dGCh'C
Cuts at: 157 936 1277 1312 1923 2016 2090 2280 2342
Size: 779 341 35 611 93 74 190 62
Cuts at: 2342 3072 157
Size: 730 1383
Fragments arranged by size:
1383 779 730 611 341 190 93 74
62 35
BspEI T'CCGG_A
Cuts at: 3518 3518
Size: 4298
BspHI T'CATG_A
Cuts at: 3474 3474
Size: 4298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1348 1562 1864 2245 1348
Size: 214 302 381 3401
Fragments arranged by size:
3401 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2590 2644 4128 4293 50
Size: 2540 54 1484 165 55
Fragments arranged by size:
2540 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2210 2210
Size: 4298
BsrFI r'CCGG_y
Cuts at: 2296 2477 2686 2296
Size: 181 209 3908
Fragments arranged by size:
3908 209 181
BssSI C'ACGA_G
Cuts at: 133 2569 2927 133
Size: 2436 358 1504
Fragments arranged by size:
2436 1504 358
BstAPI GCAn_nnn'nTGC
Cuts at: 672 1675 672
Size: 1003 3295
BstBI TT'CG_AA
Cuts at: 2659 2659
Size: 4298
BstXI CCAn_nnnn'nTGG
Cuts at: 573 573
Size: 4298
BstYI r'GATC_y
Cuts at: 1 252 1194 1200 1323 1790 1813 2148 2394
Size: 251 942 6 123 467 23 335 246
Cuts at: 2394 3406 3492 3504 3850 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 942 467 449 346 335 251 246
123 86 23 12 6
Bsu36I CC'TnA_GG
Cuts at: 872 872
Size: 4298
BtgI C'CryG_G
Cuts at: 1264 1264
Size: 4298
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2248 2429 2592 2248
Size: 181 163 3954
Fragments arranged by size:
3954 181 163
BtsI GCAGTG_nn'
Cuts at: 1541 1541
Size: 4298
DraI TTT'AAA
Cuts at: 1297 1297
Size: 4298
DrdI GACnn_nn'nnGTC
Cuts at: 2004 2862 2004
Size: 858 3440
EaeI y'GGCC_r
Cuts at: 1239 1883 2057 2448 2475 3820 1239
Size: 644 174 391 27 1345 1717
Fragments arranged by size:
1717 1345 644 391 174 27
EagI C'GGCC_G
Cuts at: 1883 1883
Size: 4298
EarI CTCTTCn'nnn_
Cuts at: 734 1120 2321 2531 734
Size: 386 1201 210 2501
Fragments arranged by size:
2501 1201 386 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1162 2506 2816 2962 4108 1162
Size: 1344 310 146 1146 1352
Fragments arranged by size:
1352 1344 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1310 1310
Size: 4298
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 524 1225 2123 2555 3302 3570 229
Size: 295 701 898 432 747 268 957
Fragments arranged by size:
957 898 747 701 432 295 268
EcoNI CCTnn'n_nnAGG
Cuts at: 247 817 247
Size: 570 3728
EcoO109I rG'GnC_Cy
Cuts at: 1437 1437
Size: 4298
EcoRI G'AATT_C
Cuts at: 1302 1302
Size: 4298
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2317 2317
Size: 4298
FspI TGC'GCA
Cuts at: 2079 2079
Size: 4298
HaeII r_GCGC'y
Cuts at: 1732 1980 3002 3981 1732
Size: 248 1022 979 2049
Fragments arranged by size:
2049 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 430 462 887 919 2140 2170 2172 2202 430
Size: 32 425 32 1221 30 2 30 2526
Fragments arranged by size:
2526 1221 425 32 32 30 30 2
HincII GTy'rAC
Cuts at: 339 978 1337 339
Size: 639 359 3300
Fragments arranged by size:
3300 639 359
HindIII A'AGCT_T
Cuts at: 1282 1282
Size: 4298
KasI G'GCGC_C
Cuts at: 1728 1976 1728
Size: 248 4050
KpnI G_GTAC'C
Cuts at: 1118 1318 1118
Size: 200 4098
MfeI C'AATT_G
Cuts at: 713 713
Size: 4298
MluI A'CGCG_T
Cuts at: 3731 3731
Size: 4298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2969 3153 2969
Size: 184 4114
MscI TGG'CCA
Cuts at: 1241 2059 1241
Size: 818 3480
MslI CAynn'nnrTG
Cuts at: 2287 2414 2287
Size: 127 4171
MspA1I CmG'CkG
Cuts at: 988 1409 1723 2083 3096 3341 988
Size: 421 314 360 1013 245 1945
Fragments arranged by size:
1945 1013 421 360 314 245
NaeI GCC'GGC
Cuts at: 2479 2479
Size: 4298
NarI GG'CG_CC
Cuts at: 1729 1977 1729
Size: 248 4050
NcoI C'CATG_G
Cuts at: 1264 1264
Size: 4298
NgoMIV G'CCGG_C
Cuts at: 2477 2477
Size: 4298
NspI r_CATG'y
Cuts at: 1351 1851 2758 1351
Size: 500 907 2891
Fragments arranged by size:
2891 907 500
PasI CC'CwG_GG
Cuts at: 1177 1177
Size: 4298
PciI A'CATG_T
Cuts at: 2754 2754
Size: 4298
PflMI CCAn_nnn'nTGG
Cuts at: 351 351
Size: 4298
PmeI GTTT'AAAC
Cuts at: 1297 1297
Size: 4298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3437 3469 3437
Size: 32 4266
PstI C_TGCA'G
Cuts at: 1233 1345 2030 1233
Size: 112 685 3501
Fragments arranged by size:
3501 685 112
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4298
PvuII CAG'CTG
Cuts at: 988 1723 2083 988
Size: 735 360 3203
Fragments arranged by size:
3203 735 360
RsrII CG'GwC_CG
Cuts at: 2493 2493
Size: 4298
SacI G_AGCT'C
Cuts at: 1312 1312
Size: 4298
SalI G'TCGA_C
Cuts at: 976 1335 976
Size: 359 3939
SapI GCTCTTCn'nnn_
Cuts at: 2321 2531 2321
Size: 210 4088
SbfI CC_TGCA'GG
Cuts at: 1345 1345
Size: 4298
ScaI AGT'ACT
Cuts at: 3815 3815
Size: 4298
SfcI C'TryA_G
Cuts at: 33 838 1229 1341 2026 3019 3210 33
Size: 805 391 112 685 993 191 1121
Fragments arranged by size:
1121 993 805 685 391 191 112
SfoI GGC'GCC
Cuts at: 1730 1978 1730
Size: 248 4050
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4298
SmaI CCC'GGG
Cuts at: 1320 1320
Size: 4298
SmlI C'TyrA_G
Cuts at: 461 653 1453 2368 2860 3122 461
Size: 192 800 915 492 262 1637
Fragments arranged by size:
1637 915 800 492 262 192
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4298
SphI G_CATG'C
Cuts at: 1351 1851 1351
Size: 500 3798
StyI C'CwwG_G
Cuts at: 434 628 971 1264 1432 1786 434
Size: 194 343 293 168 354 2946
Fragments arranged by size:
2946 354 343 293 194 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 168 209 996 1881 2014 2473 2682 168
Size: 41 787 885 133 459 209 1784
Fragments arranged by size:
1784 885 787 459 209 133 41
TatI w'GTAC_w
Cuts at: 3813 3813
Size: 4298
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3815 3847 3815
Size: 32 4266
Tth111I GACn'n_nGTC
Cuts at: 2095 2095
Size: 4298
XbaI T'CTAG_A
Cuts at: 1329 1329
Size: 4298
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1793 1793
Size: 4298
XmaI C'CCGG_G
Cuts at: 1318 1318
Size: 4298
XmnI GAAnn'nnTTC
Cuts at: 1162 1162
Size: 4298
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HpaI
NdeI NheI NotI NruI NsiI PacI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI