Restriction map for
pF1KB5181
(Circular) (Six-base) MAPSORT of: pF1KB5181.seq Check: 7866 from: 1 to: 5081
pF1KB5181 5081 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 2119 2119
Size: 5081
Acc65I G'GTAC_C
Cuts at: 1294 2097 1294
Size: 803 4278
AclI AA'CG_TT
Cuts at: 4921 4921
Size: 5081
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 1259 2906 3338 3691 4353 724
Size: 535 1647 432 353 662 1452
Fragments arranged by size:
1647 1452 662 535 432 353
AfeI AGC'GCT
Cuts at: 922 922
Size: 5081
AflIII A'CryG_T
Cuts at: 2433 4233 4514 2433
Size: 1800 281 3000
Fragments arranged by size:
3000 1800 281
AleI CACnn'nnGTG
Cuts at: 285 361 1024 285
Size: 76 663 4342
Fragments arranged by size:
4342 663 76
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1226 1678 1711 1912 1226
Size: 452 33 201 4395
Fragments arranged by size:
4395 452 201 33
AlwNI CAG_nnn'CTG
Cuts at: 883 1345 1461 1550 1944 2386 3824 883
Size: 462 116 89 394 442 1438 2140
Fragments arranged by size:
2140 1438 462 442 394 116 89
ApaLI G'TGCA_C
Cuts at: 3919 3919
Size: 5081
ApoI r'AATT_y
Cuts at: 2085 4434 4690 2085
Size: 2349 256 2476
Fragments arranged by size:
2476 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5081
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5081
AvaI C'yCGr_G
Cuts at: 538 1748 1884 2101 538
Size: 1210 136 217 3518
Fragments arranged by size:
3518 1210 217 136
BamHI G'GATC_C
Cuts at: 2106 2106
Size: 5081
BanI G'GyrC_C
Cuts at: 1150 1294 2097 2511 2759 2794 1150
Size: 144 803 414 248 35 3437
Fragments arranged by size:
3437 803 414 248 144 35
BanII G_rGCy'C
Cuts at: 116 182 440 472 512 712 1241 1337 1607
Size: 66 258 32 40 200 529 96 270
Cuts at: 1607 1643 2095 3125 116
Size: 36 452 1030 2072
Fragments arranged by size:
2072 1030 529 452 270 258 200 96
66 40 36 32
BbeI G_GCGC'C
Cuts at: 2515 2763 2515
Size: 248 4833
BbsI GAAGACnn'nnnn_
Cuts at: 728 1447 728
Size: 719 4362
BbvCI CC'TCA_GC
Cuts at: 1926 1926
Size: 5081
BciVI GTATCCnnnnn_n'
Cuts at: 2979 4035 5081 2979
Size: 1056 1046 2979
Fragments arranged by size:
2979 1056 1046
BclI T'GATC_A
Cuts at: 1028 2601 1028
Size: 1573 3508
BfrBI ATG'CAT
Cuts at: 377 377
Size: 5081
BglI GCCn_nnn'nGGC
Cuts at: 872 1611 872
Size: 739 4342
BglII A'GATC_T
Cuts at: 500 788 2596 4633 500
Size: 288 1808 2037 948
Fragments arranged by size:
2037 1808 948 288
BlpI GC'TnA_GC
Cuts at: 1801 2193 1801
Size: 392 4689
Bme1580I G_kGCm'C
Cuts at: 1153 1667 1891 2706 2799 3923 1153
Size: 514 224 815 93 1124 2311
Fragments arranged by size:
2311 1124 815 514 224 93
BmgBI CAC'GTC
Cuts at: 1776 1776
Size: 5081
BmrI ACTGGGnnnn_n'
Cuts at: 195 1202 1358 2293 2374 2709 4853 195
Size: 1007 156 935 81 335 2144 423
Fragments arranged by size:
2144 1007 935 423 335 156 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 807 3448 807
Size: 2641 2440
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 1551 1690 157
Size: 1394 139 3548
Fragments arranged by size:
3548 1394 139
Bpu10I CC'TnA_GC
Cuts at: 987 1199 1926 987
Size: 212 727 4142
Fragments arranged by size:
4142 727 212
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2257 3136 3850 4148 2257
Size: 879 714 298 3190
Fragments arranged by size:
3190 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4785 4785
Size: 5081
BsaAI yAC'GTr
Cuts at: 2434 2434
Size: 5081
BsaBI GATnn'nnATC
Cuts at: 6 2595 4632 6
Size: 2589 2037 455
Fragments arranged by size:
2589 2037 455
BsaHI Gr'CG_yC
Cuts at: 2512 2760 3462 4958 2512
Size: 248 702 1496 2635
Fragments arranged by size:
2635 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1297 2270 2791 3469 3880 4027 4301 1297
Size: 973 521 678 411 147 274 2077
Fragments arranged by size:
2077 973 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 502 556 629 688 829 1075 502
Size: 54 73 59 141 246 4508
Fragments arranged by size:
4508 246 141 73 59 54
BseYI C'CCAG_C
Cuts at: 1161 1338 1383 1475 1561 1684 1814 2506 3264
Size: 177 45 92 86 123 130 692 758
Cuts at: 3264 3929 4337 1161
Size: 665 408 1905
Fragments arranged by size:
1905 758 692 665 408 177 130 123
92 86 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 781
Size: 5081
BsiEI CG_ry'CG
Cuts at: 104 649 2623 2669 3899 104
Size: 545 1974 46 1230 1286
Fragments arranged by size:
1974 1286 1230 545 46
BsiHKAI G_wGCw'C
Cuts at: 182 440 512 712 1105 1241 1643 1807 2095
Size: 258 72 200 393 136 402 164 288
Cuts at: 2095 2873 3063 3923 182
Size: 778 190 860 1340
Fragments arranged by size:
1340 860 778 402 393 288 258 200
190 164 136 72
BsmI GAATG_Cn'
Cuts at: 810 810
Size: 5081
Bsp1286I G_dGCh'C
Cuts at: 116 182 440 472 512 712 1105 1153 1241
Size: 66 258 32 40 200 393 48 88
Cuts at: 1241 1337 1607 1643 1667 1807 1891 2095 2706
Size: 96 270 36 24 140 84 204 611
Cuts at: 2706 2799 2873 3063 3125 3923 116
Size: 93 74 190 62 798 1274
Fragments arranged by size:
1274 798 611 393 270 258 204 200
190 140 96 93 88 84 74 66
62 48 40 36 32 24
BspHI T'CATG_A
Cuts at: 902 3513 902
Size: 2611 2470
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1538 2131 2345 2647 3028 1538
Size: 593 214 302 381 3591
Fragments arranged by size:
3591 593 381 302 214
BsrBI CCG'CTC
Cuts at: 50 930 3373 3427 4911 5076 50
Size: 880 2443 54 1484 165 55
Fragments arranged by size:
2443 1484 880 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2993 2993
Size: 5081
BsrFI r'CCGG_y
Cuts at: 1084 3079 3260 1084
Size: 1995 181 2905
Fragments arranged by size:
2905 1995 181
BssSI C'ACGA_G
Cuts at: 3352 4060 3352
Size: 708 4373
BstAPI GCAn_nnn'nTGC
Cuts at: 1050 1461 1550 2458 1050
Size: 411 89 908 3673
Fragments arranged by size:
3673 908 411 89
BstBI TT'CG_AA
Cuts at: 3442 3442
Size: 5081
BstEII G'GTnAC_C
Cuts at: 2008 2008
Size: 5081
BstXI CCAn_nnnn'nTGG
Cuts at: 287 366 465 690 287
Size: 79 99 225 4678
Fragments arranged by size:
4678 225 99 79
BstYI r'GATC_y
Cuts at: 1 269 500 788 995 1119 1134 2106 2573
Size: 268 231 288 207 124 15 972 467
Cuts at: 2573 2596 2931 3177 3483 3495 3581 4633 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 972 467 449 335 306 288 268
246 231 207 124 86 23 15 12
BtgI C'CryG_G
Cuts at: 107 643 2003 107
Size: 536 1360 3185
Fragments arranged by size:
3185 1360 536
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 133 3031 3212 3375 133
Size: 2898 181 163 1839
Fragments arranged by size:
2898 1839 181 163
BtsI GCAGTG_nn'
Cuts at: 1756 2324 1756
Size: 568 4513
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1468 1503 1468
Size: 35 5046
DraI TTT'AAA
Cuts at: 2080 2080
Size: 5081
DraIII CAC_nnn'GTG
Cuts at: 1593 1593
Size: 5081
DrdI GACnn_nn'nnGTC
Cuts at: 2787 4131 2787
Size: 1344 3737
EaeI y'GGCC_r
Cuts at: 646 1649 2666 2840 3231 3258 4603 646
Size: 1003 1017 174 391 27 1345 1124
Fragments arranged by size:
1345 1124 1017 1003 391 174 27
EagI C'GGCC_G
Cuts at: 646 2666 646
Size: 2020 3061
EarI CTCTTCn'nnn_
Cuts at: 376 700 790 952 1741 3104 3314 376
Size: 324 90 162 789 1363 210 2143
Fragments arranged by size:
2143 1363 789 324 210 162 90
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3289 4031 4177 4891 3289
Size: 742 146 714 3479
Fragments arranged by size:
3479 742 714 146
EcoICRI GAG'CTC
Cuts at: 180 438 510 710 1239 1641 2093 180
Size: 258 72 200 529 402 452 3168
Fragments arranged by size:
3168 529 452 402 258 200 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 724 1259 1551 1690 2906 3338 3691 4353
Size: 567 535 292 139 1216 432 353 662
Cuts at: 4353 157
Size: 885
Fragments arranged by size:
1216 885 662 567 535 432 353 292
139
EcoNI CCTnn'n_nnAGG
Cuts at: 172 538 904 1021 172
Size: 366 366 117 4232
Fragments arranged by size:
4232 366 366 117
EcoO109I rG'GnC_Cy
Cuts at: 235 897 1359 1527 1752 1941 2220 235
Size: 662 462 168 225 189 279 3096
Fragments arranged by size:
3096 662 462 279 225 189 168
EcoRI G'AATT_C
Cuts at: 2085 2085
Size: 5081
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 441 2013 3100 441
Size: 1572 1087 2422
Fragments arranged by size:
2422 1572 1087
FspI TGC'GCA
Cuts at: 2862 2862
Size: 5081
HaeII r_GCGC'y
Cuts at: 924 1574 1770 2515 2763 3993 4764 924
Size: 650 196 745 248 1230 771 1241
Fragments arranged by size:
1241 1230 771 745 650 248 196
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 664 696 699 731 1228 1260 2923 2953 2955
Size: 32 3 32 497 32 1663 30 2
Cuts at: 2955 2985 664
Size: 30 2760
Fragments arranged by size:
2760 1663 497 32 32 32 30 30
3 2
HincII GTy'rAC
Cuts at: 1068 2120 1068
Size: 1052 4029
HindIII A'AGCT_T
Cuts at: 607 607
Size: 5081
KasI G'GCGC_C
Cuts at: 2511 2759 2511
Size: 248 4833
KpnI G_GTAC'C
Cuts at: 1298 2101 1298
Size: 803 4278
MluI A'CGCG_T
Cuts at: 4514 4514
Size: 5081
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 623 975 1301 1796 3840 4024 623
Size: 352 326 495 2044 184 1680
Fragments arranged by size:
2044 1680 495 352 326 184
MscI TGG'CCA
Cuts at: 1651 2842 1651
Size: 1191 3890
MslI CAynn'nnrTG
Cuts at: 285 361 847 1024 1660 3070 3197 285
Size: 76 486 177 636 1410 127 2169
Fragments arranged by size:
2169 1410 636 486 177 127 76
MspA1I CmG'CkG
Cuts at: 521 883 1047 1412 1550 1846 2192 2506 2866
Size: 362 164 365 138 296 346 314 360
Cuts at: 2866 3650 3895 521
Size: 784 245 1707
Fragments arranged by size:
1707 784 365 362 360 346 314 296
245 164 138
NaeI GCC'GGC
Cuts at: 1086 3262 1086
Size: 2176 2905
NarI GG'CG_CC
Cuts at: 2512 2760 2512
Size: 248 4833
NcoI C'CATG_G
Cuts at: 107 2003 107
Size: 1896 3185
NgoMIV G'CCGG_C
Cuts at: 1084 3260 1084
Size: 2176 2905
NsiI A_TGCA'T
Cuts at: 379 379
Size: 5081
NspI r_CATG'y
Cuts at: 377 2134 2634 4237 377
Size: 1757 500 1603 1221
Fragments arranged by size:
1757 1603 1221 500
PasI CC'CwG_GG
Cuts at: 1096 1096
Size: 5081
PciI A'CATG_T
Cuts at: 4233 4233
Size: 5081
PfoI T'CCnGG_A
Cuts at: 1523 1523
Size: 5081
PmeI GTTT'AAAC
Cuts at: 2080 2080
Size: 5081
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 5049
PpuMI rG'GwC_Cy
Cuts at: 897 1527 1752 1941 897
Size: 630 225 189 4037
Fragments arranged by size:
4037 630 225 189
PstI C_TGCA'G
Cuts at: 1004 1454 1535 1555 1877 2128 2813 1004
Size: 450 81 20 322 251 685 3272
Fragments arranged by size:
3272 685 450 322 251 81 20
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5081
PvuII CAG'CTG
Cuts at: 521 883 1047 1550 1846 2506 2866 521
Size: 362 164 503 296 660 360 2736
Fragments arranged by size:
2736 660 503 362 360 296 164
RsrII CG'GwC_CG
Cuts at: 3276 3276
Size: 5081
SacI G_AGCT'C
Cuts at: 182 440 512 712 1241 1643 2095 182
Size: 258 72 200 529 402 452 3168
Fragments arranged by size:
3168 529 452 402 258 200 72
SalI G'TCGA_C
Cuts at: 2118 2118
Size: 5081
SanDI GG'GwC_CC
Cuts at: 1752 1752
Size: 5081
SapI GCTCTTCn'nnn_
Cuts at: 376 700 952 3104 3314 376
Size: 324 252 2152 210 2143
Fragments arranged by size:
2152 2143 324 252 210
SbfI CC_TGCA'GG
Cuts at: 1004 2128 1004
Size: 1124 3957
ScaI AGT'ACT
Cuts at: 4598 4598
Size: 5081
SfcI C'TryA_G
Cuts at: 33 516 1000 1450 1531 1551 1873 2124 2809
Size: 483 484 450 81 20 322 251 685
Cuts at: 2809 3777 3968 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 685 484 483 450 322 251
191 81 20
SfoI GGC'GCC
Cuts at: 2513 2761 2513
Size: 248 4833
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5081
SmaI CCC'GGG
Cuts at: 1750 2103 1750
Size: 353 4728
SmlI C'TyrA_G
Cuts at: 2236 3151 3865 4127 2236
Size: 915 714 262 3190
Fragments arranged by size:
3190 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5081
SphI G_CATG'C
Cuts at: 2134 2634 2134
Size: 500 4581
StuI AGG'CCT
Cuts at: 304 562 985 1130 1614 304
Size: 258 423 145 484 3771
Fragments arranged by size:
3771 484 423 258 145
StyI C'CwwG_G
Cuts at: 107 359 1853 2003 2215 2569 107
Size: 252 1494 150 212 354 2619
Fragments arranged by size:
2619 1494 354 252 212 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1036 1951 1996 2664 2797 3256 3465 1036
Size: 915 45 668 133 459 209 2652
Fragments arranged by size:
2652 915 668 459 209 133 45
TatI w'GTAC_w
Cuts at: 4596 4596
Size: 5081
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 321 353 612 644 1579 1611 4598 4630 321
Size: 32 259 32 935 32 2987 32 772
Fragments arranged by size:
2987 935 772 259 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2878 2878
Size: 5081
XbaI T'CTAG_A
Cuts at: 2112 2112
Size: 5081
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 255 2060 2576 255
Size: 1805 516 2760
Fragments arranged by size:
2760 1805 516
XmaI C'CCGG_G
Cuts at: 1748 2101 1748
Size: 353 4728
XmnI GAAnn'nnTTC
Cuts at: 1309 1309
Size: 5081
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AloI ApaI AscI
AvrII BaeI BcgI BmtI BsaXI BsiWI BsmBI BspEI
BsrGI BssHII BstZ17I Bsu36I ClaI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PflMI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI