Restriction map for
pF1KB5183
(Circular) (Six-base) MAPSORT of: pF1KB5183.seq Check: 5428 from: 1 to: 4415
pF1KB5183 4415 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 671 1453 671
Size: 782 3633
Acc65I G'GTAC_C
Cuts at: 1431 1431
Size: 4415
AclI AA'CG_TT
Cuts at: 4255 4255
Size: 4415
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 1181 2240 2672 3419 3687 316
Size: 865 1059 432 747 268 1044
Fragments arranged by size:
1059 1044 865 747 432 268
AflIII A'CryG_T
Cuts at: 1767 2871 3848 1767
Size: 1104 977 2334
Fragments arranged by size:
2334 1104 977
AgeI A'CCGG_T
Cuts at: 2803 2803
Size: 4415
AhdI GACnn_n'nnGTC
Cuts at: 891 891
Size: 4415
AleI CACnn'nnGTG
Cuts at: 724 724
Size: 4415
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1141 1141
Size: 4415
AlwNI CAG_nnn'CTG
Cuts at: 1720 3287 1720
Size: 1567 2848
ApaI G_GGCC'C
Cuts at: 132 132
Size: 4415
ApaLI G'TGCA_C
Cuts at: 1272 3185 1272
Size: 1913 2502
ApoI r'AATT_y
Cuts at: 1419 3768 4024 1419
Size: 2349 256 1810
Fragments arranged by size:
2349 1810 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4415
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4415
AvaI C'yCGr_G
Cuts at: 131 1435 131
Size: 1304 3111
BamHI G'GATC_C
Cuts at: 1043 1440 1043
Size: 397 4018
BanI G'GyrC_C
Cuts at: 1294 1431 1845 2093 2128 1294
Size: 137 414 248 35 3581
Fragments arranged by size:
3581 414 248 137 35
BanII G_rGCy'C
Cuts at: 132 1429 2459 132
Size: 1297 1030 2088
Fragments arranged by size:
2088 1297 1030
BbeI G_GCGC'C
Cuts at: 1849 2097 1849
Size: 248 4167
BbvCI CC'TCA_GC
Cuts at: 253 253
Size: 4415
BciVI GTATCCnnnnn_n'
Cuts at: 2313 3074 4415 2313
Size: 761 1341 2313
Fragments arranged by size:
2313 1341 761
BclI T'GATC_A
Cuts at: 563 1935 563
Size: 1372 3043
BglII A'GATC_T
Cuts at: 122 1930 3967 122
Size: 1808 2037 570
Fragments arranged by size:
2037 1808 570
BlpI GC'TnA_GC
Cuts at: 479 512 1527 479
Size: 33 1015 3367
Fragments arranged by size:
3367 1015 33
Bme1580I G_kGCm'C
Cuts at: 132 215 942 1276 1299 2040 2133 3189 132
Size: 83 727 334 23 741 93 1056 1358
Fragments arranged by size:
1358 1056 741 727 334 93 83 23
BmrI ACTGGGnnnn_n'
Cuts at: 209 616 932 1394 1627 1708 2043 4187 209
Size: 407 316 462 233 81 335 2144 437
Fragments arranged by size:
2144 462 437 407 335 316 233 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2782 2782
Size: 4415
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 412 781 268
Size: 144 369 3902
Fragments arranged by size:
3902 369 144
Bpu10I CC'TnA_GC
Cuts at: 253 345 1224 253
Size: 92 879 3444
Fragments arranged by size:
3444 879 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 1117 1591 2470 2962 3260 667
Size: 450 474 879 492 298 1822
Fragments arranged by size:
1822 879 492 474 450 298
BsaI GGTCTCn'nnnn_
Cuts at: 355 981 4119 355
Size: 626 3138 651
Fragments arranged by size:
3138 651 626
BsaAI yAC'GTr
Cuts at: 1768 1768
Size: 4415
BsaBI GATnn'nnATC
Cuts at: 6 1929 3966 6
Size: 1923 2037 455
Fragments arranged by size:
2037 1923 455
BsaHI Gr'CG_yC
Cuts at: 1846 2094 2796 4292 1846
Size: 248 702 1496 1969
Fragments arranged by size:
1969 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1604 2125 2803 3077 3224 3635 1604
Size: 521 678 274 147 411 2384
Fragments arranged by size:
2384 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 301 331 301
Size: 30 4385
BseYI C'CCAG_C
Cuts at: 933 1199 1356 1840 2598 3175 3671 933
Size: 266 157 484 758 577 496 1677
Fragments arranged by size:
1677 758 577 496 484 266 157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 683 683
Size: 4415
BsiEI CG_ry'CG
Cuts at: 104 1957 2003 3211 104
Size: 1853 46 1208 1308
Fragments arranged by size:
1853 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1276 1429 2207 2397 3189 1276
Size: 153 778 190 792 2502
Fragments arranged by size:
2502 792 778 190 153
Bsp1286I G_dGCh'C
Cuts at: 132 215 942 1276 1299 1429 2040 2133 2207
Size: 83 727 334 23 130 611 93 74
Cuts at: 2207 2397 2459 3189 132
Size: 190 62 730 1358
Fragments arranged by size:
1358 730 727 611 334 190 130 93
83 74 62 23
BspEI T'CCGG_A
Cuts at: 3635 3635
Size: 4415
BspHI T'CATG_A
Cuts at: 611 1337 3591 611
Size: 726 2254 1435
Fragments arranged by size:
2254 1435 726
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1465 1679 1981 2362 1465
Size: 214 302 381 3518
Fragments arranged by size:
3518 381 302 214
BsrBI CCG'CTC
Cuts at: 50 483 1095 2707 2761 4245 4410 50
Size: 433 612 1612 54 1484 165 55
Fragments arranged by size:
1612 1484 612 433 165 55 54
BsrDI GCAATG_nn'
Cuts at: 506 1247 2327 506
Size: 741 1080 2594
Fragments arranged by size:
2594 1080 741
BsrFI r'CCGG_y
Cuts at: 549 2413 2594 2803 549
Size: 1864 181 209 2161
Fragments arranged by size:
2161 1864 209 181
BssSI C'ACGA_G
Cuts at: 2686 3044 2686
Size: 358 4057
BstAPI GCAn_nnn'nTGC
Cuts at: 1792 1792
Size: 4415
BstBI TT'CG_AA
Cuts at: 2776 2776
Size: 4415
BstXI CCAn_nnnn'nTGG
Cuts at: 726 759 1195 1407 726
Size: 33 436 212 3734
Fragments arranged by size:
3734 436 212 33
BstYI r'GATC_y
Cuts at: 1 122 137 174 1043 1440 1907 1930 2265
Size: 121 15 37 869 397 467 23 335
Cuts at: 2265 2511 3523 3609 3621 3967 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 869 467 449 397 346 335 246
121 86 37 23 15 12
BtgI C'CryG_G
Cuts at: 150 150
Size: 4415
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 309 1204 2365 2546 2709 309
Size: 895 1161 181 163 2015
Fragments arranged by size:
2015 1161 895 181 163
BtsI GCAGTG_nn'
Cuts at: 215 308 1106 1658 215
Size: 93 798 552 2972
Fragments arranged by size:
2972 798 552 93
DraI TTT'AAA
Cuts at: 1414 1414
Size: 4415
DrdI GACnn_nn'nnGTC
Cuts at: 2121 2979 2121
Size: 858 3557
EaeI y'GGCC_r
Cuts at: 455 518 596 2000 2174 2565 2592 3937 455
Size: 63 78 1404 174 391 27 1345 933
Fragments arranged by size:
1404 1345 933 391 174 78 63 27
EagI C'GGCC_G
Cuts at: 2000 2000
Size: 4415
EarI CTCTTCn'nnn_
Cuts at: 1346 2438 2648 1346
Size: 1092 210 3113
Fragments arranged by size:
3113 1092 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2623 2933 3079 4225 2623
Size: 310 146 1146 2813
Fragments arranged by size:
2813 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1427 1427
Size: 4415
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 316 412 781 1181 2240 2672 3419 3687
Size: 48 96 369 400 1059 432 747 268
Cuts at: 3687 268
Size: 996
Fragments arranged by size:
1059 996 747 432 400 369 268 96
48
EcoO109I rG'GnC_Cy
Cuts at: 243 987 1554 243
Size: 744 567 3104
Fragments arranged by size:
3104 744 567
EcoRI G'AATT_C
Cuts at: 1419 1419
Size: 4415
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2434 2434
Size: 4415
FspI TGC'GCA
Cuts at: 2196 2196
Size: 4415
HaeII r_GCGC'y
Cuts at: 1849 2097 3119 4098 1849
Size: 248 1022 979 2166
Fragments arranged by size:
2166 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 400 432 877 909 2257 2287 2289 2319 400
Size: 32 445 32 1348 30 2 30 2496
Fragments arranged by size:
2496 1348 445 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1140 1454 1140
Size: 314 4101
KasI G'GCGC_C
Cuts at: 1845 2093 1845
Size: 248 4167
KpnI G_GTAC'C
Cuts at: 1435 1435
Size: 4415
MluI A'CGCG_T
Cuts at: 3848 3848
Size: 4415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 377 3086 3270 377
Size: 2709 184 1522
Fragments arranged by size:
2709 1522 184
MscI TGG'CCA
Cuts at: 598 2176 598
Size: 1578 2837
MslI CAynn'nnrTG
Cuts at: 724 2404 2531 724
Size: 1680 127 2608
Fragments arranged by size:
2608 1680 127
MspA1I CmG'CkG
Cuts at: 152 478 516 933 1526 1840 2200 3213 3458
Size: 326 38 417 593 314 360 1013 245
Cuts at: 3458 152
Size: 1109
Fragments arranged by size:
1109 1013 593 417 360 326 314 245
38
NaeI GCC'GGC
Cuts at: 551 2596 551
Size: 2045 2370
NarI GG'CG_CC
Cuts at: 1846 2094 1846
Size: 248 4167
NgoMIV G'CCGG_C
Cuts at: 549 2594 549
Size: 2045 2370
NspI r_CATG'y
Cuts at: 1468 1968 2875 1468
Size: 500 907 3008
Fragments arranged by size:
3008 907 500
PciI A'CATG_T
Cuts at: 2871 2871
Size: 4415
PflMI CCAn_nnn'nTGG
Cuts at: 1053 1053
Size: 4415
PfoI T'CCnGG_A
Cuts at: 140 758 140
Size: 618 3797
PmeI GTTT'AAAC
Cuts at: 1414 1414
Size: 4415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3554 3586 3554
Size: 32 4383
PpuMI rG'GwC_Cy
Cuts at: 987 987
Size: 4415
PspOMI G'GGCC_C
Cuts at: 128 128
Size: 4415
PstI C_TGCA'G
Cuts at: 210 1462 2147 210
Size: 1252 685 2478
Fragments arranged by size:
2478 1252 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4415
PvuII CAG'CTG
Cuts at: 478 516 933 1840 2200 478
Size: 38 417 907 360 2693
Fragments arranged by size:
2693 907 417 360 38
RsrII CG'GwC_CG
Cuts at: 2610 2610
Size: 4415
SacI G_AGCT'C
Cuts at: 1429 1429
Size: 4415
SacII CC_GC'GG
Cuts at: 153 153
Size: 4415
SalI G'TCGA_C
Cuts at: 1452 1452
Size: 4415
SapI GCTCTTCn'nnn_
Cuts at: 1346 2438 2648 1346
Size: 1092 210 3113
Fragments arranged by size:
3113 1092 210
SbfI CC_TGCA'GG
Cuts at: 1462 1462
Size: 4415
ScaI AGT'ACT
Cuts at: 3932 3932
Size: 4415
SexAI A'CCwGG_T
Cuts at: 1256 1256
Size: 4415
SfcI C'TryA_G
Cuts at: 33 206 1458 2143 3136 3327 33
Size: 173 1252 685 993 191 1121
Fragments arranged by size:
1252 1121 993 685 191 173
SfoI GGC'GCC
Cuts at: 1847 2095 1847
Size: 248 4167
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4415
SmaI CCC'GGG
Cuts at: 133 1437 133
Size: 1304 3111
SmlI C'TyrA_G
Cuts at: 682 1132 1570 2485 2977 3239 682
Size: 450 438 915 492 262 1858
Fragments arranged by size:
1858 915 492 450 438 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4415
SphI G_CATG'C
Cuts at: 1468 1968 1468
Size: 500 3915
StuI AGG'CCT
Cuts at: 1114 1114
Size: 4415
StyI C'CwwG_G
Cuts at: 776 1549 1903 776
Size: 773 354 3288
Fragments arranged by size:
3288 773 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 433 1045 1143 1998 2131 2590 2799 433
Size: 612 98 855 133 459 209 2049
Fragments arranged by size:
2049 855 612 459 209 133 98
TatI w'GTAC_w
Cuts at: 3930 3930
Size: 4415
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 301 333 3932 3964 301
Size: 32 3599 32 752
Fragments arranged by size:
3599 752 32 32
Tth111I GACn'n_nGTC
Cuts at: 955 2212 955
Size: 1257 3158
XbaI T'CTAG_A
Cuts at: 1446 1446
Size: 4415
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 168 438 1288 1910 168
Size: 270 850 622 2673
Fragments arranged by size:
2673 850 622 270
XmaI C'CCGG_G
Cuts at: 131 1435 131
Size: 1304 3111
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI AscI AvrII BaeI
BbsI BcgI BfrBI BglI BmgBI BmtI BseRI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PshAI PsiI PspXI PsrI SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI