Restriction map for
pF1KB5184
(Circular) (Six-base) MAPSORT of: pF1KB5184.seq Check: 5325 from: 1 to: 4931
pF1KB5184 4931 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 351 351
Size: 4931
AccI GT'mk_AC
Cuts at: 361 1969 361
Size: 1608 3323
Acc65I G'GTAC_C
Cuts at: 1947 1947
Size: 4931
AclI AA'CG_TT
Cuts at: 4771 4771
Size: 4931
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 176 932 1045 1601 1797 2756 3188 3541
Size: 30 756 113 556 196 959 432 353
Cuts at: 3541 4203 146
Size: 662 874
Fragments arranged by size:
959 874 756 662 556 432 353 196
113 30
AfeI AGC'GCT
Cuts at: 1012 1012
Size: 4931
AflIII A'CryG_T
Cuts at: 2283 4083 4364 2283
Size: 1800 281 2850
Fragments arranged by size:
2850 1800 281
AleI CACnn'nnGTG
Cuts at: 1051 1051
Size: 4931
AlwNI CAG_nnn'CTG
Cuts at: 195 390 2236 3674 195
Size: 195 1846 1438 1452
Fragments arranged by size:
1846 1452 1438 195
ApaI G_GGCC'C
Cuts at: 786 822 1652 786
Size: 36 830 4065
Fragments arranged by size:
4065 830 36
ApaLI G'TGCA_C
Cuts at: 3769 3769
Size: 4931
ApoI r'AATT_y
Cuts at: 1935 4284 4540 1935
Size: 2349 256 2326
Fragments arranged by size:
2349 2326 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4931
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4931
AvaI C'yCGr_G
Cuts at: 332 926 1951 332
Size: 594 1025 3312
Fragments arranged by size:
3312 1025 594
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 371 404 491 524 371
Size: 33 87 33 4778
Fragments arranged by size:
4778 87 33 33
BamHI G'GATC_C
Cuts at: 1506 1697 1956 1506
Size: 191 259 4481
Fragments arranged by size:
4481 259 191
BanI G'GyrC_C
Cuts at: 339 1756 1822 1879 1903 1947 2361 2609 2644
Size: 1417 66 57 24 44 414 248 35
Cuts at: 2644 339
Size: 2626
Fragments arranged by size:
2626 1417 414 248 66 57 44 35
24
BanII G_rGCy'C
Cuts at: 786 822 1169 1611 1628 1652 1856 1945 2975
Size: 36 347 442 17 24 204 89 1030
Cuts at: 2975 786
Size: 2742
Fragments arranged by size:
2742 1030 442 347 204 89 36 24
17
BbeI G_GCGC'C
Cuts at: 2365 2613 2365
Size: 248 4683
BbvCI CC'TCA_GC
Cuts at: 1568 1568
Size: 4931
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 112 146 1608 1642 112
Size: 34 1462 34 3401
Fragments arranged by size:
3401 1462 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 600 1310 2829 3885 4931 600
Size: 710 1519 1056 1046 600
Fragments arranged by size:
1519 1056 1046 710 600
BclI T'GATC_A
Cuts at: 681 2451 681
Size: 1770 3161
BglI GCCn_nnn'nGGC
Cuts at: 247 352 745 754 781 790 247
Size: 105 393 9 27 9 4388
Fragments arranged by size:
4388 393 105 27 9 9
BglII A'GATC_T
Cuts at: 2446 4483 2446
Size: 2037 2894
BlpI GC'TnA_GC
Cuts at: 2043 2043
Size: 4931
Bme1580I G_kGCm'C
Cuts at: 786 822 1652 2556 2649 3773 786
Size: 36 830 904 93 1124 1944
Fragments arranged by size:
1944 1124 904 830 93 36
BmgBI CAC'GTC
Cuts at: 1341 1341
Size: 4931
BmrI ACTGGGnnnn_n'
Cuts at: 582 1195 1668 2143 2224 2559 4703 582
Size: 613 473 475 81 335 2144 810
Fragments arranged by size:
2144 810 613 475 473 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 850 3298 850
Size: 2448 2483
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 877 274
Size: 603 4328
Bpu10I CC'TnA_GC
Cuts at: 521 566 1568 521
Size: 45 1002 3884
Fragments arranged by size:
3884 1002 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1462 2107 2986 3700 3998 1462
Size: 645 879 714 298 2395
Fragments arranged by size:
2395 879 714 645 298
BsaI GGTCTCn'nnnn_
Cuts at: 264 282 300 318 466 726 1450 4635 264
Size: 18 18 18 148 260 724 3185 560
Fragments arranged by size:
3185 724 560 260 148 18 18 18
BsaAI yAC'GTr
Cuts at: 2284 2284
Size: 4931
BsaBI GATnn'nnATC
Cuts at: 6 1003 2445 4482 6
Size: 997 1442 2037 455
Fragments arranged by size:
2037 1442 997 455
BsaHI Gr'CG_yC
Cuts at: 2362 2610 3312 4808 2362
Size: 248 702 1496 2485
Fragments arranged by size:
2485 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2120 2641 3319 3730 3877 4151 2120
Size: 521 678 411 147 274 2900
Fragments arranged by size:
2900 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 552 1212 2356 3114 3779 4187 552
Size: 660 1144 758 665 408 1296
Fragments arranged by size:
1296 1144 758 665 660 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 521 599 152
Size: 369 78 4484
Fragments arranged by size:
4484 369 78
BsiEI CG_ry'CG
Cuts at: 104 2473 2519 3749 104
Size: 2369 46 1230 1286
Fragments arranged by size:
2369 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1611 1945 2723 2913 3773 1611
Size: 334 778 190 860 2769
Fragments arranged by size:
2769 860 778 334 190
BsmI GAATG_Cn'
Cuts at: 441 1644 441
Size: 1203 3728
Bsp1286I G_dGCh'C
Cuts at: 786 822 1169 1611 1628 1652 1856 1945 2556
Size: 36 347 442 17 24 204 89 611
Cuts at: 2556 2649 2723 2913 2975 3773 786
Size: 93 74 190 62 798 1944
Fragments arranged by size:
1944 798 611 442 347 204 190 93
89 74 62 36 24 17
BspHI T'CATG_A
Cuts at: 1247 3363 1247
Size: 2116 2815
BspMI ACCTGCnnnn'nnnn_
Cuts at: 124 351 1981 2195 2497 2878 124
Size: 227 1630 214 302 381 2177
Fragments arranged by size:
2177 1630 381 302 227 214
BsrBI CCG'CTC
Cuts at: 50 3223 3277 4761 4926 50
Size: 3173 54 1484 165 55
Fragments arranged by size:
3173 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2843 2843
Size: 4931
BsrFI r'CCGG_y
Cuts at: 2929 3110 2929
Size: 181 4750
BsrGI T'GTAC_A
Cuts at: 1142 1142
Size: 4931
BssSI C'ACGA_G
Cuts at: 3202 3910 3202
Size: 708 4223
BstAPI GCAn_nnn'nTGC
Cuts at: 2308 2308
Size: 4931
BstBI TT'CG_AA
Cuts at: 3292 3292
Size: 4931
BstXI CCAn_nnnn'nTGG
Cuts at: 1738 1738
Size: 4931
BstYI r'GATC_y
Cuts at: 1 1506 1697 1956 2423 2446 2781 3027 3333
Size: 1505 191 259 467 23 335 246 306
Cuts at: 3333 3345 3431 4483 1
Size: 12 86 1052 449
Fragments arranged by size:
1505 1052 467 449 335 306 259 246
191 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1555 2881 3062 3225 1555
Size: 1326 181 163 3261
Fragments arranged by size:
3261 1326 181 163
BtsI GCAGTG_nn'
Cuts at: 684 1749 2174 684
Size: 1065 425 3441
Fragments arranged by size:
3441 1065 425
DraI TTT'AAA
Cuts at: 1930 1930
Size: 4931
DrdI GACnn_nn'nnGTC
Cuts at: 2637 3981 2637
Size: 1344 3587
EaeI y'GGCC_r
Cuts at: 711 2516 2690 3081 3108 4453 711
Size: 1805 174 391 27 1345 1189
Fragments arranged by size:
1805 1345 1189 391 174 27
EagI C'GGCC_G
Cuts at: 2516 2516
Size: 4931
EarI CTCTTCn'nnn_
Cuts at: 659 1861 2954 3164 659
Size: 1202 1093 210 2426
Fragments arranged by size:
2426 1202 1093 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3139 3881 4027 4741 3139
Size: 742 146 714 3329
Fragments arranged by size:
3329 742 714 146
EcoICRI GAG'CTC
Cuts at: 1609 1943 1609
Size: 334 4597
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 176 274 877 932 1045 1601 1797 2756
Size: 30 98 603 55 113 556 196 959
Cuts at: 2756 3188 3541 4203 146
Size: 432 353 662 874
Fragments arranged by size:
959 874 662 603 556 432 353 196
113 98 55 30
EcoO109I rG'GnC_Cy
Cuts at: 222 549 766 1437 1649 2070 222
Size: 327 217 671 212 421 3083
Fragments arranged by size:
3083 671 421 327 217 212
EcoRI G'AATT_C
Cuts at: 1935 1935
Size: 4931
EcoRV GAT'ATC
Cuts at: 1674 1674
Size: 4931
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2950 2950
Size: 4931
FspI TGC'GCA
Cuts at: 1294 2712 1294
Size: 1418 3513
HaeII r_GCGC'y
Cuts at: 1014 2365 2613 3843 4614 1014
Size: 1351 248 1230 771 1331
Fragments arranged by size:
1351 1331 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 177 195 209 1593 1625 1824 1856 2773
Size: 14 18 14 1384 32 199 32 917
Cuts at: 2773 2803 2805 2835 163
Size: 30 2 30 2259
Fragments arranged by size:
2259 1384 917 199 32 32 30 30
18 14 14 2
HincII GTy'rAC
Cuts at: 200 1970 200
Size: 1770 3161
HindIII A'AGCT_T
Cuts at: 158 158
Size: 4931
KasI G'GCGC_C
Cuts at: 2361 2609 2361
Size: 248 4683
KpnI G_GTAC'C
Cuts at: 1951 1951
Size: 4931
MfeI C'AATT_G
Cuts at: 1111 1111
Size: 4931
MluI A'CGCG_T
Cuts at: 4364 4364
Size: 4931
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3690 3874 3690
Size: 184 4747
MscI TGG'CCA
Cuts at: 2692 2692
Size: 4931
MslI CAynn'nnrTG
Cuts at: 656 1051 1246 2920 3047 656
Size: 395 195 1674 127 2540
Fragments arranged by size:
2540 1674 395 195 127
MspA1I CmG'CkG
Cuts at: 372 397 418 583 1567 1579 2042 2356 2716
Size: 25 21 165 984 12 463 314 360
Cuts at: 2716 3500 3745 372
Size: 784 245 1558
Fragments arranged by size:
1558 984 784 463 360 314 245 165
25 21 12
NaeI GCC'GGC
Cuts at: 3112 3112
Size: 4931
NarI GG'CG_CC
Cuts at: 2362 2610 2362
Size: 248 4683
NgoMIV G'CCGG_C
Cuts at: 3110 3110
Size: 4931
NspI r_CATG'y
Cuts at: 1023 1984 2484 4087 1023
Size: 961 500 1603 1867
Fragments arranged by size:
1867 1603 961 500
PasI CC'CwG_GG
Cuts at: 1312 1682 1312
Size: 370 4561
PciI A'CATG_T
Cuts at: 4083 4083
Size: 4931
PflMI CCAn_nnn'nTGG
Cuts at: 216 390 1517 216
Size: 174 1127 3630
Fragments arranged by size:
3630 1127 174
PmeI GTTT'AAAC
Cuts at: 1930 1930
Size: 4931
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3377 3409 3377
Size: 32 4899
PpuMI rG'GwC_Cy
Cuts at: 222 766 222
Size: 544 4387
PspOMI G'GGCC_C
Cuts at: 782 818 1648 782
Size: 36 830 4065
Fragments arranged by size:
4065 830 36
PstI C_TGCA'G
Cuts at: 1566 1578 1978 2663 1566
Size: 12 400 685 3834
Fragments arranged by size:
3834 685 400 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4931
PvuII CAG'CTG
Cuts at: 372 397 418 583 1567 1579 2356 2716 372
Size: 25 21 165 984 12 777 360 2587
Fragments arranged by size:
2587 984 777 360 165 25 21 12
RsrII CG'GwC_CG
Cuts at: 3126 3126
Size: 4931
SacI G_AGCT'C
Cuts at: 1611 1945 1611
Size: 334 4597
SalI G'TCGA_C
Cuts at: 1968 1968
Size: 4931
SanDI GG'GwC_CC
Cuts at: 766 766
Size: 4931
SapI GCTCTTCn'nnn_
Cuts at: 1861 2954 3164 1861
Size: 1093 210 3628
Fragments arranged by size:
3628 1093 210
SbfI CC_TGCA'GG
Cuts at: 1978 1978
Size: 4931
ScaI AGT'ACT
Cuts at: 1764 4448 1764
Size: 2684 2247
SfcI C'TryA_G
Cuts at: 33 1562 1574 1974 2659 3627 3818 33
Size: 1529 12 400 685 968 191 1146
Fragments arranged by size:
1529 1146 968 685 400 191 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 745 745
Size: 4931
SfoI GGC'GCC
Cuts at: 2363 2611 2363
Size: 248 4683
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4931
SmaI CCC'GGG
Cuts at: 1953 1953
Size: 4931
SmlI C'TyrA_G
Cuts at: 1477 2086 3001 3715 3977 1477
Size: 609 915 714 262 2431
Fragments arranged by size:
2431 915 714 609 262
SpeI A'CTAG_T
Cuts at: 67 1765 67
Size: 1698 3233
SphI G_CATG'C
Cuts at: 1984 2484 1984
Size: 500 4431
StuI AGG'CCT
Cuts at: 241 355 241
Size: 114 4817
StyI C'CwwG_G
Cuts at: 617 1511 1586 1866 2065 2419 617
Size: 894 75 280 199 354 3129
Fragments arranged by size:
3129 894 354 280 199 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 364 1221 1250 2514 2647 3106 3315 364
Size: 857 29 1264 133 459 209 1980
Fragments arranged by size:
1980 1264 857 459 209 133 29
TatI w'GTAC_w
Cuts at: 1142 1762 4446 1142
Size: 620 2684 1627
Fragments arranged by size:
2684 1627 620
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4448 4480 4448
Size: 32 4899
Tth111I GACn'n_nGTC
Cuts at: 2728 2728
Size: 4931
XbaI T'CTAG_A
Cuts at: 1962 1962
Size: 4931
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2426 2426
Size: 4931
XmaI C'CCGG_G
Cuts at: 1951 1951
Size: 4931
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AgeI AhdI AlfI AloI AscI AvrII
BbsI BfrBI BmtI BsaXI BseRI BsiWI BsmBI BspEI
BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI FseI FspAI HpaI NcoI NdeI NheI NotI
NruI NsiI PacI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SexAI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI