Restriction map for
pF1KB5195
(Circular) (Six-base) MAPSORT of: pF1KB5195.seq Check: 5465 from: 1 to: 3827
pF1KB5195 3827 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 865 865
Size: 3827
Acc65I G'GTAC_C
Cuts at: 843 843
Size: 3827
AclI AA'CG_TT
Cuts at: 3667 3667
Size: 3827
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1652 2084 2831 3099 1652
Size: 432 747 268 2380
Fragments arranged by size:
2380 747 432 268
AflIII A'CryG_T
Cuts at: 1179 2283 3260 1179
Size: 1104 977 1746
Fragments arranged by size:
1746 1104 977
AgeI A'CCGG_T
Cuts at: 2215 2215
Size: 3827
AlwNI CAG_nnn'CTG
Cuts at: 1132 2699 1132
Size: 1567 2260
ApaLI G'TGCA_C
Cuts at: 2597 2597
Size: 3827
ApoI r'AATT_y
Cuts at: 831 3180 3436 831
Size: 2349 256 1222
Fragments arranged by size:
2349 1222 256
AseI AT'TA_AT
Cuts at: 20 620 644 20
Size: 600 24 3203
Fragments arranged by size:
3203 600 24
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3827
AvaI C'yCGr_G
Cuts at: 847 847
Size: 3827
BamHI G'GATC_C
Cuts at: 852 852
Size: 3827
BanI G'GyrC_C
Cuts at: 843 1257 1505 1540 843
Size: 414 248 35 3130
Fragments arranged by size:
3130 414 248 35
BanII G_rGCy'C
Cuts at: 841 1871 841
Size: 1030 2797
BbeI G_GCGC'C
Cuts at: 1261 1509 1261
Size: 248 3579
BbsI GAAGACnn'nnnn_
Cuts at: 285 285
Size: 3827
BbvCI CC'TCA_GC
Cuts at: 703 703
Size: 3827
BciVI GTATCCnnnnn_n'
Cuts at: 1725 2486 3827 1725
Size: 761 1341 1725
Fragments arranged by size:
1725 1341 761
BclI T'GATC_A
Cuts at: 356 1347 356
Size: 991 2836
BglII A'GATC_T
Cuts at: 1342 3379 1342
Size: 2037 1790
BlpI GC'TnA_GC
Cuts at: 939 939
Size: 3827
Bme1580I G_kGCm'C
Cuts at: 213 1452 1545 2601 213
Size: 1239 93 1056 1439
Fragments arranged by size:
1439 1239 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1039 1120 1455 3599 1039
Size: 81 335 2144 1267
Fragments arranged by size:
2144 1267 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2194 2194
Size: 3827
Bpu10I CC'TnA_GC
Cuts at: 703 703
Size: 3827
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1003 1882 2374 2672 1003
Size: 879 492 298 2158
Fragments arranged by size:
2158 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3531 3531
Size: 3827
BsaAI yAC'GTr
Cuts at: 1180 1180
Size: 3827
BsaBI GATnn'nnATC
Cuts at: 6 1341 3378 6
Size: 1335 2037 455
Fragments arranged by size:
2037 1335 455
BsaHI Gr'CG_yC
Cuts at: 1258 1506 2208 3704 1258
Size: 248 702 1496 1381
Fragments arranged by size:
1496 1381 702 248
BsaWI w'CCGG_w
Cuts at: 1016 1537 2215 2489 2636 3047 1016
Size: 521 678 274 147 411 1796
Fragments arranged by size:
1796 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1252 2010 2587 3083 1252
Size: 758 577 496 1996
Fragments arranged by size:
1996 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 200
Size: 3827
BsiEI CG_ry'CG
Cuts at: 104 1369 1415 2623 104
Size: 1265 46 1208 1308
Fragments arranged by size:
1308 1265 1208 46
BsiHKAI G_wGCw'C
Cuts at: 841 1619 1809 2601 841
Size: 778 190 792 2067
Fragments arranged by size:
2067 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 213 841 1452 1545 1619 1809 1871 2601 213
Size: 628 611 93 74 190 62 730 1439
Fragments arranged by size:
1439 730 628 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3047 3047
Size: 3827
BspHI T'CATG_A
Cuts at: 3003 3003
Size: 3827
BspMI ACCTGCnnnn'nnnn_
Cuts at: 877 1091 1393 1774 877
Size: 214 302 381 2930
Fragments arranged by size:
2930 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2119 2173 3657 3822 50
Size: 2069 54 1484 165 55
Fragments arranged by size:
2069 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 410 1739 410
Size: 1329 2498
BsrFI r'CCGG_y
Cuts at: 1825 2006 2215 1825
Size: 181 209 3437
Fragments arranged by size:
3437 209 181
BssSI C'ACGA_G
Cuts at: 787 2098 2456 787
Size: 1311 358 2158
Fragments arranged by size:
2158 1311 358
BstAPI GCAn_nnn'nTGC
Cuts at: 412 1204 412
Size: 792 3035
BstBI TT'CG_AA
Cuts at: 2188 2188
Size: 3827
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3827
BstYI r'GATC_y
Cuts at: 1 852 1319 1342 1677 1923 2935 3021 3033
Size: 851 467 23 335 246 1012 86 12
Cuts at: 3033 3379 1
Size: 346 449
Fragments arranged by size:
1012 851 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 197 107
Size: 90 3737
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 264 1777 1958 2121 264
Size: 1513 181 163 1970
Fragments arranged by size:
1970 1513 181 163
BtsI GCAGTG_nn'
Cuts at: 188 290 500 1070 188
Size: 102 210 570 2945
Fragments arranged by size:
2945 570 210 102
DraI TTT'AAA
Cuts at: 826 826
Size: 3827
DrdI GACnn_nn'nnGTC
Cuts at: 1533 2391 1533
Size: 858 2969
EaeI y'GGCC_r
Cuts at: 401 1412 1586 1977 2004 3349 401
Size: 1011 174 391 27 1345 879
Fragments arranged by size:
1345 1011 879 391 174 27
EagI C'GGCC_G
Cuts at: 1412 1412
Size: 3827
EarI CTCTTCn'nnn_
Cuts at: 304 787 1850 2060 304
Size: 483 1063 210 2071
Fragments arranged by size:
2071 1063 483 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2035 2345 2491 3637 2035
Size: 310 146 1146 2225
Fragments arranged by size:
2225 1146 310 146
EcoICRI GAG'CTC
Cuts at: 839 839
Size: 3827
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1652 2084 2831 3099 1652
Size: 432 747 268 2380
Fragments arranged by size:
2380 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 329 966 329
Size: 637 3190
EcoRI G'AATT_C
Cuts at: 831 831
Size: 3827
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1846 1846
Size: 3827
FspI TGC'GCA
Cuts at: 1608 1608
Size: 3827
HaeII r_GCGC'y
Cuts at: 1261 1509 2531 3510 1261
Size: 248 1022 979 1578
Fragments arranged by size:
1578 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1669 1699 1701 1731 1669
Size: 30 2 30 3765
Fragments arranged by size:
3765 30 30 2
HincII GTy'rAC
Cuts at: 866 866
Size: 3827
KasI G'GCGC_C
Cuts at: 1257 1505 1257
Size: 248 3579
KpnI G_GTAC'C
Cuts at: 847 847
Size: 3827
MluI A'CGCG_T
Cuts at: 3260 3260
Size: 3827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2498 2682 2498
Size: 184 3643
MscI TGG'CCA
Cuts at: 1588 1588
Size: 3827
MslI CAynn'nnrTG
Cuts at: 112 283 391 1816 1943 112
Size: 171 108 1425 127 1996
Fragments arranged by size:
1996 1425 171 127 108
MspA1I CmG'CkG
Cuts at: 938 1252 1612 2625 2870 938
Size: 314 360 1013 245 1895
Fragments arranged by size:
1895 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2008 2008
Size: 3827
NarI GG'CG_CC
Cuts at: 1258 1506 1258
Size: 248 3579
NcoI C'CATG_G
Cuts at: 107 107
Size: 3827
NgoMIV G'CCGG_C
Cuts at: 2006 2006
Size: 3827
NspI r_CATG'y
Cuts at: 880 1380 2287 880
Size: 500 907 2420
Fragments arranged by size:
2420 907 500
PciI A'CATG_T
Cuts at: 2283 2283
Size: 3827
PfoI T'CCnGG_A
Cuts at: 512 512
Size: 3827
PmeI GTTT'AAAC
Cuts at: 826 826
Size: 3827
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2966 2998 2966
Size: 32 3795
PstI C_TGCA'G
Cuts at: 732 874 1559 732
Size: 142 685 3000
Fragments arranged by size:
3000 685 142
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3827
PvuII CAG'CTG
Cuts at: 1252 1612 1252
Size: 360 3467
RsrII CG'GwC_CG
Cuts at: 2022 2022
Size: 3827
SacI G_AGCT'C
Cuts at: 841 841
Size: 3827
SalI G'TCGA_C
Cuts at: 864 864
Size: 3827
SapI GCTCTTCn'nnn_
Cuts at: 1850 2060 1850
Size: 210 3617
SbfI CC_TGCA'GG
Cuts at: 874 874
Size: 3827
ScaI AGT'ACT
Cuts at: 3344 3344
Size: 3827
SfcI C'TryA_G
Cuts at: 33 728 870 1555 2548 2739 33
Size: 695 142 685 993 191 1121
Fragments arranged by size:
1121 993 695 685 191 142
SfoI GGC'GCC
Cuts at: 1259 1507 1259
Size: 248 3579
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3827
SmaI CCC'GGG
Cuts at: 849 849
Size: 3827
SmlI C'TyrA_G
Cuts at: 982 1897 2389 2651 982
Size: 915 492 262 2158
Fragments arranged by size:
2158 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3827
SphI G_CATG'C
Cuts at: 880 1380 880
Size: 500 3327
StyI C'CwwG_G
Cuts at: 107 738 961 1315 107
Size: 631 223 354 2619
Fragments arranged by size:
2619 631 354 223
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 190 277 1410 1543 2002 2211 190
Size: 87 1133 133 459 209 1806
Fragments arranged by size:
1806 1133 459 209 133 87
TatI w'GTAC_w
Cuts at: 3342 3342
Size: 3827
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 203 235 3344 3376 203
Size: 32 3109 32 654
Fragments arranged by size:
3109 654 32 32
Tth111I GACn'n_nGTC
Cuts at: 1624 1624
Size: 3827
XbaI T'CTAG_A
Cuts at: 858 858
Size: 3827
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1322 1322
Size: 3827
XmaI C'CCGG_G
Cuts at: 847 847
Size: 3827
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BpmI BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI