Restriction map for
pF1KB5205
(Circular) (Six-base) MAPSORT of: pF1KB5205.seq Check: 8159 from: 1 to: 4742
pF1KB5205 4742 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 417 417
Size: 4742
AccI GT'mk_AC
Cuts at: 347 1538 1780 347
Size: 1191 242 3309
Fragments arranged by size:
3309 1191 242
Acc65I G'GTAC_C
Cuts at: 1758 1758
Size: 4742
AclI AA'CG_TT
Cuts at: 4582 4582
Size: 4742
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 1084 2567 2999 3352 4014 442
Size: 642 1483 432 353 662 1170
Fragments arranged by size:
1483 1170 662 642 432 353
AflIII A'CryG_T
Cuts at: 1028 2094 3894 4175 1028
Size: 1066 1800 281 1595
Fragments arranged by size:
1800 1595 1066 281
AleI CACnn'nnGTG
Cuts at: 394 394
Size: 4742
AlwNI CAG_nnn'CTG
Cuts at: 191 1404 1627 2047 3485 191
Size: 1213 223 420 1438 1448
Fragments arranged by size:
1448 1438 1213 420 223
ApaLI G'TGCA_C
Cuts at: 3580 3580
Size: 4742
ApoI r'AATT_y
Cuts at: 1746 4095 4351 1746
Size: 2349 256 2137
Fragments arranged by size:
2349 2137 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4742
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4742
AvaI C'yCGr_G
Cuts at: 721 838 1762 721
Size: 117 924 3701
Fragments arranged by size:
3701 924 117
BamHI G'GATC_C
Cuts at: 1767 1767
Size: 4742
BanI G'GyrC_C
Cuts at: 385 1237 1758 2172 2420 2455 385
Size: 852 521 414 248 35 2672
Fragments arranged by size:
2672 852 521 414 248 35
BanII G_rGCy'C
Cuts at: 601 720 839 1506 1756 2786 601
Size: 119 119 667 250 1030 2557
Fragments arranged by size:
2557 1030 667 250 119 119
BbeI G_GCGC'C
Cuts at: 1241 2176 2424 1241
Size: 935 248 3559
Fragments arranged by size:
3559 935 248
BbsI GAAGACnn'nnnn_
Cuts at: 766 766
Size: 4742
BbvCI CC'TCA_GC
Cuts at: 206 371 206
Size: 165 4577
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1540 1574 1540
Size: 34 4708
BciVI GTATCCnnnnn_n'
Cuts at: 2640 3696 4742 2640
Size: 1056 1046 2640
Fragments arranged by size:
2640 1056 1046
BclI T'GATC_A
Cuts at: 2262 2262
Size: 4742
BglI GCCn_nnn'nGGC
Cuts at: 1630 1630
Size: 4742
BglII A'GATC_T
Cuts at: 2257 4294 2257
Size: 2037 2705
BlpI GC'TnA_GC
Cuts at: 1854 1854
Size: 4742
Bme1580I G_kGCm'C
Cuts at: 2367 2460 3584 2367
Size: 93 1124 3525
Fragments arranged by size:
3525 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1002 1954 2035 2370 4514 1002
Size: 952 81 335 2144 1230
Fragments arranged by size:
2144 1230 952 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3109 3109
Size: 4742
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 980 980
Size: 4742
Bpu10I CC'TnA_GC
Cuts at: 206 371 206
Size: 165 4577
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1362 1918 2797 3511 3809 1362
Size: 556 879 714 298 2295
Fragments arranged by size:
2295 879 714 556 298
BsaI GGTCTCn'nnnn_
Cuts at: 1150 4446 1150
Size: 3296 1446
BsaAI yAC'GTr
Cuts at: 2095 2095
Size: 4742
BsaBI GATnn'nnATC
Cuts at: 6 694 2256 4293 6
Size: 688 1562 2037 455
Fragments arranged by size:
2037 1562 688 455
BsaHI Gr'CG_yC
Cuts at: 1238 2173 2421 3123 4619 1238
Size: 935 248 702 1496 1361
Fragments arranged by size:
1496 1361 935 702 248
BsaWI w'CCGG_w
Cuts at: 1931 2452 3130 3541 3688 3962 1931
Size: 521 678 411 147 274 2711
Fragments arranged by size:
2711 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 953 983 953
Size: 30 4712
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1069 1270 1069
Size: 201 4541
BseYI C'CCAG_C
Cuts at: 167 494 614 775 1131 2167 2925 3590 3998
Size: 327 120 161 356 1036 758 665 408
Cuts at: 3998 167
Size: 911
Fragments arranged by size:
1036 911 758 665 408 356 327 161
120
BsiEI CG_ry'CG
Cuts at: 104 2284 2330 3560 104
Size: 2180 46 1230 1286
Fragments arranged by size:
2180 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 601 839 1756 2534 2724 3584 601
Size: 238 917 778 190 860 1759
Fragments arranged by size:
1759 917 860 778 238 190
BsmI GAATG_Cn'
Cuts at: 751 1116 751
Size: 365 4377
Bsp1286I G_dGCh'C
Cuts at: 601 720 839 1506 1756 2367 2460 2534 2724
Size: 119 119 667 250 611 93 74 190
Cuts at: 2724 2786 3584 601
Size: 62 798 1759
Fragments arranged by size:
1759 798 667 611 250 190 119 119
93 74 62
BspHI T'CATG_A
Cuts at: 1286 3174 1286
Size: 1888 2854
BspMI ACCTGCnnnn'nnnn_
Cuts at: 417 1373 1792 2006 2308 2689 417
Size: 956 419 214 302 381 2470
Fragments arranged by size:
2470 956 419 381 302 214
BsrBI CCG'CTC
Cuts at: 50 592 1266 1679 3034 3088 4572 4737 50
Size: 542 674 413 1355 54 1484 165 55
Fragments arranged by size:
1484 1355 674 542 413 165 55 54
BsrDI GCAATG_nn'
Cuts at: 350 1556 2654 350
Size: 1206 1098 2438
Fragments arranged by size:
2438 1206 1098
BsrFI r'CCGG_y
Cuts at: 2740 2921 2740
Size: 181 4561
BssSI C'ACGA_G
Cuts at: 3013 3721 3013
Size: 708 4034
BstAPI GCAn_nnn'nTGC
Cuts at: 2119 2119
Size: 4742
BstBI TT'CG_AA
Cuts at: 3103 3103
Size: 4742
BstXI CCAn_nnnn'nTGG
Cuts at: 498 958 1347 1431 498
Size: 460 389 84 3809
Fragments arranged by size:
3809 460 389 84
BstYI r'GATC_y
Cuts at: 1 947 1059 1767 2234 2257 2592 2838 3144
Size: 946 112 708 467 23 335 246 306
Cuts at: 3144 3156 3242 4294 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 946 708 467 449 335 306 246
112 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1052 1052
Size: 4742
BtgI C'CryG_G
Cuts at: 234 1230 1716 234
Size: 996 486 3260
Fragments arranged by size:
3260 996 486
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2692 2873 3036 2692
Size: 181 163 4398
Fragments arranged by size:
4398 181 163
BtsI GCAGTG_nn'
Cuts at: 659 1985 659
Size: 1326 3416
DraI TTT'AAA
Cuts at: 1741 1741
Size: 4742
DrdI GACnn_nn'nnGTC
Cuts at: 1266 2448 3792 1266
Size: 1182 1344 2216
Fragments arranged by size:
2216 1344 1182
EaeI y'GGCC_r
Cuts at: 1001 1041 1725 2327 2501 2892 2919 4264 1001
Size: 40 684 602 174 391 27 1345 1479
Fragments arranged by size:
1479 1345 684 602 391 174 40 27
EagI C'GGCC_G
Cuts at: 2327 2327
Size: 4742
EarI CTCTTCn'nnn_
Cuts at: 1060 1148 2765 2975 1060
Size: 88 1617 210 2827
Fragments arranged by size:
2827 1617 210 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2950 3692 3838 4552 2950
Size: 742 146 714 3140
Fragments arranged by size:
3140 742 714 146
EcoICRI GAG'CTC
Cuts at: 599 837 1754 599
Size: 238 917 3587
Fragments arranged by size:
3587 917 238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 980 1084 2567 2999 3352 4014 442
Size: 538 104 1483 432 353 662 1170
Fragments arranged by size:
1483 1170 662 538 432 353 104
EcoO109I rG'GnC_Cy
Cuts at: 229 1881 229
Size: 1652 3090
EcoRI G'AATT_C
Cuts at: 1746 1746
Size: 4742
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2761 2761
Size: 4742
FspI TGC'GCA
Cuts at: 2523 2523
Size: 4742
HaeII r_GCGC'y
Cuts at: 1241 2176 2424 3654 4425 1241
Size: 935 248 1230 771 1558
Fragments arranged by size:
1558 1230 935 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1079 1111 1526 1558 2584 2614 2616 2646 1079
Size: 32 415 32 1026 30 2 30 3175
Fragments arranged by size:
3175 1026 415 32 32 30 30 2
HincII GTy'rAC
Cuts at: 507 1781 507
Size: 1274 3468
HindIII A'AGCT_T
Cuts at: 247 623 1574 247
Size: 376 951 3415
Fragments arranged by size:
3415 951 376
HpaI GTT'AAC
Cuts at: 507 507
Size: 4742
KasI G'GCGC_C
Cuts at: 1237 2172 2420 1237
Size: 935 248 3559
Fragments arranged by size:
3559 935 248
KpnI G_GTAC'C
Cuts at: 1762 1762
Size: 4742
MfeI C'AATT_G
Cuts at: 902 902
Size: 4742
MluI A'CGCG_T
Cuts at: 4175 4175
Size: 4742
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3501 3685 3501
Size: 184 4558
MscI TGG'CCA
Cuts at: 1003 2503 1003
Size: 1500 3242
MslI CAynn'nnrTG
Cuts at: 394 1345 2731 2858 394
Size: 951 1386 127 2278
Fragments arranged by size:
2278 1386 951 127
MspA1I CmG'CkG
Cuts at: 498 735 1404 1452 1853 2167 2527 3311 3556
Size: 237 669 48 401 314 360 784 245
Cuts at: 3556 498
Size: 1684
Fragments arranged by size:
1684 784 669 401 360 314 245 237
48
NaeI GCC'GGC
Cuts at: 2923 2923
Size: 4742
NarI GG'CG_CC
Cuts at: 1238 2173 2421 1238
Size: 935 248 3559
Fragments arranged by size:
3559 935 248
NgoMIV G'CCGG_C
Cuts at: 2921 2921
Size: 4742
NspI r_CATG'y
Cuts at: 1032 1217 1513 1795 2295 3898 1032
Size: 185 296 282 500 1603 1876
Fragments arranged by size:
1876 1603 500 296 282 185
PasI CC'CwG_GG
Cuts at: 225 1515 225
Size: 1290 3452
PciI A'CATG_T
Cuts at: 1028 3894 1028
Size: 2866 1876
PfoI T'CCnGG_A
Cuts at: 1389 1389
Size: 4742
PmeI GTTT'AAAC
Cuts at: 1741 1741
Size: 4742
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1663 1695 3188 3220 1663
Size: 32 1493 32 3185
Fragments arranged by size:
3185 1493 32 32
PpuMI rG'GwC_Cy
Cuts at: 229 229
Size: 4742
PspXI vC'TCGA_Gb
Cuts at: 721 838 721
Size: 117 4625
PstI C_TGCA'G
Cuts at: 333 414 680 1789 2474 333
Size: 81 266 1109 685 2601
Fragments arranged by size:
2601 1109 685 266 81
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4742
PvuII CAG'CTG
Cuts at: 498 735 1404 1452 2167 2527 498
Size: 237 669 48 715 360 2713
Fragments arranged by size:
2713 715 669 360 237 48
RsrII CG'GwC_CG
Cuts at: 2937 2937
Size: 4742
SacI G_AGCT'C
Cuts at: 601 839 1756 601
Size: 238 917 3587
Fragments arranged by size:
3587 917 238
SalI G'TCGA_C
Cuts at: 1779 1779
Size: 4742
SanDI GG'GwC_CC
Cuts at: 229 229
Size: 4742
SapI GCTCTTCn'nnn_
Cuts at: 2765 2975 2765
Size: 210 4532
SbfI CC_TGCA'GG
Cuts at: 1789 1789
Size: 4742
ScaI AGT'ACT
Cuts at: 1012 4259 1012
Size: 3247 1495
SfcI C'TryA_G
Cuts at: 33 329 410 676 1652 1785 2470 3438 3629
Size: 296 81 266 976 133 685 968 191
Cuts at: 3629 33
Size: 1146
Fragments arranged by size:
1146 976 968 685 296 266 191 133
81
SfoI GGC'GCC
Cuts at: 1239 2174 2422 1239
Size: 935 248 3559
Fragments arranged by size:
3559 935 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4742
SmaI CCC'GGG
Cuts at: 1764 1764
Size: 4742
SmlI C'TyrA_G
Cuts at: 721 838 1377 1897 2812 3526 3788 721
Size: 117 539 520 915 714 262 1675
Fragments arranged by size:
1675 915 714 539 520 262 117
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4742
SphI G_CATG'C
Cuts at: 1513 1795 2295 1513
Size: 282 500 3960
Fragments arranged by size:
3960 500 282
StuI AGG'CCT
Cuts at: 1429 1429
Size: 4742
StyI C'CwwG_G
Cuts at: 156 1202 1424 1430 1876 2230 156
Size: 1046 222 6 446 354 2668
Fragments arranged by size:
2668 1046 446 354 222 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 367 508 1021 2325 2458 2917 3126 367
Size: 141 513 1304 133 459 209 1983
Fragments arranged by size:
1983 1304 513 459 209 141 133
TatI w'GTAC_w
Cuts at: 1010 4257 1010
Size: 3247 1495
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 110 142 4259 4291 110
Size: 32 4117 32 561
Fragments arranged by size:
4117 561 32 32
Tth111I GACn'n_nGTC
Cuts at: 2539 2539
Size: 4742
XbaI T'CTAG_A
Cuts at: 1773 1773
Size: 4742
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 996 2237 996
Size: 1241 3501
XhoI C'TCGA_G
Cuts at: 721 838 721
Size: 117 4625
XmaI C'CCGG_G
Cuts at: 1762 1762
Size: 4742
XmnI GAAnn'nnTTC
Cuts at: 625 763 625
Size: 138 4604
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PpuMI PspXI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI ApaI
AscI AvrII BaeI BfrBI BmgBI BmtI BsgI BsiWI
BsmBI BspEI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI NcoI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PshAI PsiI
PspOMI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI ZraI