Restriction map for
pF1KB5210
(Circular) (Six-base) MAPSORT of: pF1KB5210.seq Check: 8163 from: 1 to: 4097
pF1KB5210 4097 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 390 390
Size: 4097
AccI GT'mk_AC
Cuts at: 188 500 1135 188
Size: 312 635 3150
Fragments arranged by size:
3150 635 312
Acc65I G'GTAC_C
Cuts at: 1113 1113
Size: 4097
AclI AA'CG_TT
Cuts at: 3937 3937
Size: 4097
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 826 857 1922 2354 3101 3369 583
Size: 243 31 1065 432 747 268 1311
Fragments arranged by size:
1311 1065 747 432 268 243 31
AflIII A'CryG_T
Cuts at: 1449 2553 3530 1449
Size: 1104 977 2016
Fragments arranged by size:
2016 1104 977
AgeI A'CCGG_T
Cuts at: 2485 2485
Size: 4097
AleI CACnn'nnGTG
Cuts at: 361 361
Size: 4097
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 293 293
Size: 4097
AlwNI CAG_nnn'CTG
Cuts at: 645 804 1402 2969 645
Size: 159 598 1567 1773
Fragments arranged by size:
1773 1567 598 159
ApaLI G'TGCA_C
Cuts at: 278 2867 278
Size: 2589 1508
ApoI r'AATT_y
Cuts at: 1101 3450 3706 1101
Size: 2349 256 1492
Fragments arranged by size:
2349 1492 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4097
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
AvaI C'yCGr_G
Cuts at: 489 1117 489
Size: 628 3469
BamHI G'GATC_C
Cuts at: 1122 1122
Size: 4097
BanI G'GyrC_C
Cuts at: 178 366 415 697 1113 1527 1775 1810 178
Size: 188 49 282 416 414 248 35 2465
Fragments arranged by size:
2465 416 414 282 248 188 49 35
BanII G_rGCy'C
Cuts at: 798 911 1111 2141 798
Size: 113 200 1030 2754
Fragments arranged by size:
2754 1030 200 113
BbeI G_GCGC'C
Cuts at: 1531 1779 1531
Size: 248 3849
BbsI GAAGACnn'nnnn_
Cuts at: 142 790 142
Size: 648 3449
BciVI GTATCCnnnnn_n'
Cuts at: 1995 2756 4097 1995
Size: 761 1341 1995
Fragments arranged by size:
1995 1341 761
BclI T'GATC_A
Cuts at: 1617 1617
Size: 4097
BglI GCCn_nnn'nGGC
Cuts at: 919 919
Size: 4097
BglII A'GATC_T
Cuts at: 1612 3649 1612
Size: 2037 2060
BlpI GC'TnA_GC
Cuts at: 1209 1209
Size: 4097
Bme1580I G_kGCm'C
Cuts at: 210 248 282 371 702 1722 1815 2871 210
Size: 38 34 89 331 1020 93 1056 1436
Fragments arranged by size:
1436 1056 1020 331 93 89 38 34
BmgBI CAC'GTC
Cuts at: 669 669
Size: 4097
BmrI ACTGGGnnnn_n'
Cuts at: 1309 1390 1725 3869 1309
Size: 81 335 2144 1537
Fragments arranged by size:
2144 1537 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 4097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 988 137
Size: 851 3246
Bpu10I CC'TnA_GC
Cuts at: 797 797
Size: 4097
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 388 1039 1273 2152 2644 2942 178
Size: 210 651 234 879 492 298 1333
Fragments arranged by size:
1333 879 651 492 298 234 210
BsaI GGTCTCn'nnnn_
Cuts at: 3801 3801
Size: 4097
BsaAI yAC'GTr
Cuts at: 132 948 1450 132
Size: 816 502 2779
Fragments arranged by size:
2779 816 502
BsaBI GATnn'nnATC
Cuts at: 6 1611 3648 6
Size: 1605 2037 455
Fragments arranged by size:
2037 1605 455
BsaHI Gr'CG_yC
Cuts at: 347 1528 1776 2478 3974 347
Size: 1181 248 702 1496 470
Fragments arranged by size:
1496 1181 702 470 248
BsaWI w'CCGG_w
Cuts at: 1286 1807 2485 2759 2906 3317 1286
Size: 521 678 274 147 411 2066
Fragments arranged by size:
2066 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 559 589 559
Size: 30 4067
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 285 285
Size: 4097
BseYI C'CCAG_C
Cuts at: 209 370 659 1040 1065 1522 2280 2857 3353
Size: 161 289 381 25 457 758 577 496
Cuts at: 3353 209
Size: 953
Fragments arranged by size:
953 758 577 496 457 381 289 161
25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 112 112
Size: 4097
BsiEI CG_ry'CG
Cuts at: 104 1639 1685 2893 104
Size: 1535 46 1208 1308
Fragments arranged by size:
1535 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 282 1111 1889 2079 2871 282
Size: 829 778 190 792 1508
Fragments arranged by size:
1508 829 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 210 248 282 371 702 798 911 1111 1722
Size: 38 34 89 331 96 113 200 611
Cuts at: 1722 1815 1889 2079 2141 2871 210
Size: 93 74 190 62 730 1436
Fragments arranged by size:
1436 730 611 331 200 190 113 96
93 89 74 62 38 34
BspEI T'CCGG_A
Cuts at: 3317 3317
Size: 4097
BspHI T'CATG_A
Cuts at: 3273 3273
Size: 4097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 390 1147 1361 1663 2044 390
Size: 757 214 302 381 2443
Fragments arranged by size:
2443 757 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2389 2443 3927 4092 50
Size: 2339 54 1484 165 55
Fragments arranged by size:
2339 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2009 2009
Size: 4097
BsrFI r'CCGG_y
Cuts at: 175 2095 2276 2485 175
Size: 1920 181 209 1787
Fragments arranged by size:
1920 1787 209 181
BssSI C'ACGA_G
Cuts at: 1000 2368 2726 1000
Size: 1368 358 2371
Fragments arranged by size:
2371 1368 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1474 1474
Size: 4097
BstBI TT'CG_AA
Cuts at: 2458 2458
Size: 4097
BstXI CCAn_nnnn'nTGG
Cuts at: 363 774 363
Size: 411 3686
BstYI r'GATC_y
Cuts at: 1 230 896 1122 1589 1612 1947 2193 3205
Size: 229 666 226 467 23 335 246 1012
Cuts at: 3205 3291 3303 3649 1
Size: 86 12 346 449
Fragments arranged by size:
1012 666 467 449 346 335 246 229
226 86 23 12
BtgI C'CryG_G
Cuts at: 683 683
Size: 4097
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 579 663 2047 2228 2391 579
Size: 84 1384 181 163 2285
Fragments arranged by size:
2285 1384 181 163 84
BtsI GCAGTG_nn'
Cuts at: 1340 1340
Size: 4097
DraI TTT'AAA
Cuts at: 1096 1096
Size: 4097
DrdI GACnn_nn'nnGTC
Cuts at: 673 1803 2661 673
Size: 1130 858 2109
Fragments arranged by size:
2109 1130 858
EaeI y'GGCC_r
Cuts at: 173 332 464 863 929 1682 1856 2247 2274
Size: 159 132 399 66 753 174 391 27
Cuts at: 2274 3619 173
Size: 1345 651
Fragments arranged by size:
1345 753 651 399 391 174 159 132
66 27
EagI C'GGCC_G
Cuts at: 1682 1682
Size: 4097
EarI CTCTTCn'nnn_
Cuts at: 2120 2330 2120
Size: 210 3887
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2305 2615 2761 3907 2305
Size: 310 146 1146 2495
Fragments arranged by size:
2495 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1109 1109
Size: 4097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 583 826 857 988 1922 2354 3101 3369
Size: 446 243 31 131 934 432 747 268
Cuts at: 3369 137
Size: 865
Fragments arranged by size:
934 865 747 446 432 268 243 131
31
EcoO109I rG'GnC_Cy
Cuts at: 471 618 771 801 1236 471
Size: 147 153 30 435 3332
Fragments arranged by size:
3332 435 153 147 30
EcoRI G'AATT_C
Cuts at: 1101 1101
Size: 4097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 4097
FspI TGC'GCA
Cuts at: 1878 1878
Size: 4097
HaeII r_GCGC'y
Cuts at: 402 1054 1531 1779 2801 3780 402
Size: 652 477 248 1022 979 719
Fragments arranged by size:
1022 979 719 652 477 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1939 1969 1971 2001 1939
Size: 30 2 30 4035
Fragments arranged by size:
4035 30 30 2
HincII GTy'rAC
Cuts at: 1136 1136
Size: 4097
KasI G'GCGC_C
Cuts at: 1527 1775 1527
Size: 248 3849
KpnI G_GTAC'C
Cuts at: 1117 1117
Size: 4097
MluI A'CGCG_T
Cuts at: 3530 3530
Size: 4097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 987 2768 2952 987
Size: 1781 184 2132
Fragments arranged by size:
2132 1781 184
MscI TGG'CCA
Cuts at: 466 1858 466
Size: 1392 2705
MslI CAynn'nnrTG
Cuts at: 361 364 682 2086 2213 361
Size: 3 318 1404 127 2245
Fragments arranged by size:
2245 1404 318 127 3
MspA1I CmG'CkG
Cuts at: 934 990 1044 1065 1208 1522 1882 2895 3140
Size: 56 54 21 143 314 360 1013 245
Cuts at: 3140 934
Size: 1891
Fragments arranged by size:
1891 1013 360 314 245 143 56 54
21
NaeI GCC'GGC
Cuts at: 177 2278 177
Size: 2101 1996
NarI GG'CG_CC
Cuts at: 1528 1776 1528
Size: 248 3849
NcoI C'CATG_G
Cuts at: 683 683
Size: 4097
NdeI CA'TA_TG
Cuts at: 327 327
Size: 4097
NgoMIV G'CCGG_C
Cuts at: 175 2276 175
Size: 2101 1996
NspI r_CATG'y
Cuts at: 1150 1650 2557 1150
Size: 500 907 2690
Fragments arranged by size:
2690 907 500
PasI CC'CwG_GG
Cuts at: 774 774
Size: 4097
PciI A'CATG_T
Cuts at: 2553 2553
Size: 4097
PmeI GTTT'AAAC
Cuts at: 1096 1096
Size: 4097
PmlI CAC'GTG
Cuts at: 132 948 132
Size: 816 3281
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 4065
PstI C_TGCA'G
Cuts at: 650 1144 1829 650
Size: 494 685 2918
Fragments arranged by size:
2918 685 494
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4097
PvuII CAG'CTG
Cuts at: 1044 1065 1522 1882 1044
Size: 21 457 360 3259
Fragments arranged by size:
3259 457 360 21
RsrII CG'GwC_CG
Cuts at: 2292 2292
Size: 4097
SacI G_AGCT'C
Cuts at: 1111 1111
Size: 4097
SalI G'TCGA_C
Cuts at: 1134 1134
Size: 4097
SapI GCTCTTCn'nnn_
Cuts at: 2120 2330 2120
Size: 210 3887
SbfI CC_TGCA'GG
Cuts at: 1144 1144
Size: 4097
ScaI AGT'ACT
Cuts at: 3614 3614
Size: 4097
SfcI C'TryA_G
Cuts at: 33 646 939 1140 1825 2818 3009 33
Size: 613 293 201 685 993 191 1121
Fragments arranged by size:
1121 993 685 613 293 201 191
SfoI GGC'GCC
Cuts at: 1529 1777 1529
Size: 248 3849
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
SmaI CCC'GGG
Cuts at: 491 1119 491
Size: 628 3469
SmlI C'TyrA_G
Cuts at: 193 403 1054 1252 2167 2659 2921 193
Size: 210 651 198 915 492 262 1369
Fragments arranged by size:
1369 915 651 492 262 210 198
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4097
SphI G_CATG'C
Cuts at: 1150 1650 1150
Size: 500 3597
StyI C'CwwG_G
Cuts at: 467 683 767 1088 1231 1585 467
Size: 216 84 321 143 354 2979
Fragments arranged by size:
2979 354 321 216 143 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 424 1680 1813 2272 2481 424
Size: 1256 133 459 209 2040
Fragments arranged by size:
2040 1256 459 209 133
TatI w'GTAC_w
Cuts at: 3612 3612
Size: 4097
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 559 591 3614 3646 559
Size: 32 3023 32 1010
Fragments arranged by size:
3023 1010 32 32
Tth111I GACn'n_nGTC
Cuts at: 1894 1894
Size: 4097
XbaI T'CTAG_A
Cuts at: 1128 1128
Size: 4097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 426 1592 426
Size: 1166 2931
XmaI C'CCGG_G
Cuts at: 489 1117 489
Size: 628 3469
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PmeI PmlI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PflMI PfoI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI