Restriction map for
pF1KB5211
(Circular) (Six-base) MAPSORT of: pF1KB5211.seq Check: 4448 from: 1 to: 4247
pF1KB5211 4247 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 412 412
Size: 4247
AatII G_ACGT'C
Cuts at: 509 1027 509
Size: 518 3729
AccI GT'mk_AC
Cuts at: 1285 1285
Size: 4247
Acc65I G'GTAC_C
Cuts at: 1263 1263
Size: 4247
AclI AA'CG_TT
Cuts at: 530 4087 530
Size: 3557 690
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 1238 2072 2504 2857 3519 811
Size: 427 834 432 353 662 1539
Fragments arranged by size:
1539 834 662 432 427 353
AfeI AGC'GCT
Cuts at: 976 976
Size: 4247
AflIII A'CryG_T
Cuts at: 589 1009 1599 3399 3680 589
Size: 420 590 1800 281 1156
Fragments arranged by size:
1800 1156 590 420 281
AlwNI CAG_nnn'CTG
Cuts at: 1552 2990 1552
Size: 1438 2809
ApaI G_GGCC'C
Cuts at: 498 498
Size: 4247
ApaLI G'TGCA_C
Cuts at: 3085 3085
Size: 4247
ApoI r'AATT_y
Cuts at: 811 946 1251 3600 3856 811
Size: 135 305 2349 256 1202
Fragments arranged by size:
2349 1202 305 256 135
AseI AT'TA_AT
Cuts at: 20 20
Size: 4247
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4247
AvaI C'yCGr_G
Cuts at: 489 929 1267 489
Size: 440 338 3469
Fragments arranged by size:
3469 440 338
BamHI G'GATC_C
Cuts at: 386 1272 386
Size: 886 3361
BanI G'GyrC_C
Cuts at: 1263 1677 1925 1960 1263
Size: 414 248 35 3550
Fragments arranged by size:
3550 414 248 35
BanII G_rGCy'C
Cuts at: 498 930 1261 2291 498
Size: 432 331 1030 2454
Fragments arranged by size:
2454 1030 432 331
BbeI G_GCGC'C
Cuts at: 1681 1929 1681
Size: 248 3999
BbsI GAAGACnn'nnnn_
Cuts at: 553 1062 553
Size: 509 3738
BciVI GTATCCnnnnn_n'
Cuts at: 694 2145 3201 4247 694
Size: 1451 1056 1046 694
Fragments arranged by size:
1451 1056 1046 694
BclI T'GATC_A
Cuts at: 1767 1767
Size: 4247
BglII A'GATC_T
Cuts at: 1762 3799 1762
Size: 2037 2210
BlpI GC'TnA_GC
Cuts at: 215 1359 215
Size: 1144 3103
Bme1580I G_kGCm'C
Cuts at: 498 1872 1965 3089 498
Size: 1374 93 1124 1656
Fragments arranged by size:
1656 1374 1124 93
BmgBI CAC'GTC
Cuts at: 456 456
Size: 4247
BmrI ACTGGGnnnn_n'
Cuts at: 1459 1540 1875 4019 1459
Size: 81 335 2144 1687
Fragments arranged by size:
2144 1687 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2614 2614
Size: 4247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 612 981 1219 612
Size: 369 238 3640
Fragments arranged by size:
3640 369 238
Bpu10I CC'TnA_GC
Cuts at: 581 581
Size: 4247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 486 972 1423 2302 3016 3314 486
Size: 486 451 879 714 298 1419
Fragments arranged by size:
1419 879 714 486 451 298
BsaI GGTCTCn'nnnn_
Cuts at: 3951 3951
Size: 4247
BsaAI yAC'GTr
Cuts at: 383 592 1012 1600 383
Size: 209 420 588 3030
Fragments arranged by size:
3030 588 420 209
BsaBI GATnn'nnATC
Cuts at: 6 1761 3798 6
Size: 1755 2037 455
Fragments arranged by size:
2037 1755 455
BsaHI Gr'CG_yC
Cuts at: 272 506 543 1024 1678 1926 2628 4124 272
Size: 234 37 481 654 248 702 1496 395
Fragments arranged by size:
1496 702 654 481 395 248 234 37
BsaWI w'CCGG_w
Cuts at: 1436 1957 2635 3046 3193 3467 1436
Size: 521 678 411 147 274 2216
Fragments arranged by size:
2216 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 865 895 865
Size: 30 4217
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1009 1216 1009
Size: 207 4040
BseYI C'CCAG_C
Cuts at: 476 971 1041 1672 2430 3095 3503 476
Size: 495 70 631 758 665 408 1220
Fragments arranged by size:
1220 758 665 631 495 408 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 675 1252 675
Size: 577 3670
BsiEI CG_ry'CG
Cuts at: 104 1789 1835 3065 104
Size: 1685 46 1230 1286
Fragments arranged by size:
1685 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 967 1261 2039 2229 3089 967
Size: 294 778 190 860 2125
Fragments arranged by size:
2125 860 778 294 190
BsmI GAATG_Cn'
Cuts at: 848 848
Size: 4247
Bsp1286I G_dGCh'C
Cuts at: 498 930 967 1261 1872 1965 2039 2229 2291
Size: 432 37 294 611 93 74 190 62
Cuts at: 2291 3089 498
Size: 798 1656
Fragments arranged by size:
1656 798 611 432 294 190 93 74
62 37
BspHI T'CATG_A
Cuts at: 450 2679 450
Size: 2229 2018
BspMI ACCTGCnnnn'nnnn_
Cuts at: 412 448 1297 1511 1813 2194 412
Size: 36 849 214 302 381 2465
Fragments arranged by size:
2465 849 381 302 214 36
BsrBI CCG'CTC
Cuts at: 50 2539 2593 4077 4242 50
Size: 2489 54 1484 165 55
Fragments arranged by size:
2489 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2159 2159
Size: 4247
BsrFI r'CCGG_y
Cuts at: 288 1192 2245 2426 288
Size: 904 1053 181 2109
Fragments arranged by size:
2109 1053 904 181
BssSI C'ACGA_G
Cuts at: 2518 3226 2518
Size: 708 3539
BstAPI GCAn_nnn'nTGC
Cuts at: 1624 1624
Size: 4247
BstBI TT'CG_AA
Cuts at: 2608 2608
Size: 4247
BstYI r'GATC_y
Cuts at: 1 171 386 1272 1739 1762 2097 2343 2649
Size: 170 215 886 467 23 335 246 306
Cuts at: 2649 2661 2747 3799 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 886 467 449 335 306 246 215
170 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 196 196
Size: 4247
BtgI C'CryG_G
Cuts at: 107 500 107
Size: 393 3854
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2197 2378 2541 2197
Size: 181 163 3903
Fragments arranged by size:
3903 181 163
BtsI GCAGTG_nn'
Cuts at: 299 1490 299
Size: 1191 3056
DraI TTT'AAA
Cuts at: 1246 1246
Size: 4247
DrdI GACnn_nn'nnGTC
Cuts at: 1953 3297 1953
Size: 1344 2903
EaeI y'GGCC_r
Cuts at: 362 1832 2006 2397 2424 3769 362
Size: 1470 174 391 27 1345 840
Fragments arranged by size:
1470 1345 840 391 174 27
EagI C'GGCC_G
Cuts at: 1832 1832
Size: 4247
EarI CTCTTCn'nnn_
Cuts at: 397 2270 2480 397
Size: 1873 210 2164
Fragments arranged by size:
2164 1873 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1035 2455 3197 3343 4057 1035
Size: 1420 742 146 714 1225
Fragments arranged by size:
1420 1225 742 714 146
EcoICRI GAG'CTC
Cuts at: 1259 1259
Size: 4247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 612 811 981 1219 1238 2072 2504 2857 3519
Size: 199 170 238 19 834 432 353 662
Cuts at: 3519 612
Size: 1340
Fragments arranged by size:
1340 834 662 432 353 238 199 170
19
EcoNI CCTnn'n_nnAGG
Cuts at: 419 419
Size: 4247
EcoO109I rG'GnC_Cy
Cuts at: 231 494 495 1386 231
Size: 263 1 891 3092
Fragments arranged by size:
3092 891 263 1
EcoRI G'AATT_C
Cuts at: 946 1251 946
Size: 305 3942
EcoRV GAT'ATC
Cuts at: 255 255
Size: 4247
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2266 2266
Size: 4247
FspI TGC'GCA
Cuts at: 2028 2028
Size: 4247
HaeII r_GCGC'y
Cuts at: 959 978 1681 1929 3159 3930 959
Size: 19 703 248 1230 771 1276
Fragments arranged by size:
1276 1230 771 703 248 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 601 633 717 749 865 897 2089 2119 2121
Size: 32 84 32 116 32 1192 30 2
Cuts at: 2121 2151 601
Size: 30 2697
Fragments arranged by size:
2697 1192 116 84 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 224 547 1286 224
Size: 323 739 3185
Fragments arranged by size:
3185 739 323
KasI G'GCGC_C
Cuts at: 1677 1925 1677
Size: 248 3999
KpnI G_GTAC'C
Cuts at: 1267 1267
Size: 4247
MluI A'CGCG_T
Cuts at: 3680 3680
Size: 4247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3006 3190 3006
Size: 184 4063
MscI TGG'CCA
Cuts at: 364 2008 364
Size: 1644 2603
MslI CAynn'nnrTG
Cuts at: 655 2236 2363 655
Size: 1581 127 2539
Fragments arranged by size:
2539 1581 127
MspA1I CmG'CkG
Cuts at: 476 502 632 1358 1672 2032 2816 3061 476
Size: 26 130 726 314 360 784 245 1662
Fragments arranged by size:
1662 784 726 360 314 245 130 26
NaeI GCC'GGC
Cuts at: 290 1194 2428 290
Size: 904 1234 2109
Fragments arranged by size:
2109 1234 904
NarI GG'CG_CC
Cuts at: 1678 1926 1678
Size: 248 3999
NcoI C'CATG_G
Cuts at: 107 107
Size: 4247
NgoMIV G'CCGG_C
Cuts at: 288 1192 2426 288
Size: 904 1234 2109
Fragments arranged by size:
2109 1234 904
NspI r_CATG'y
Cuts at: 1300 1800 3403 1300
Size: 500 1603 2144
Fragments arranged by size:
2144 1603 500
PciI A'CATG_T
Cuts at: 3399 3399
Size: 4247
PflMI CCAn_nnn'nTGG
Cuts at: 654 654
Size: 4247
PfoI T'CCnGG_A
Cuts at: 900 900
Size: 4247
PmeI GTTT'AAAC
Cuts at: 1246 1246
Size: 4247
PmlI CAC'GTG
Cuts at: 383 1012 383
Size: 629 3618
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2693 2725 2693
Size: 32 4215
PpuMI rG'GwC_Cy
Cuts at: 231 231
Size: 4247
PspOMI G'GGCC_C
Cuts at: 494 494
Size: 4247
PstI C_TGCA'G
Cuts at: 475 1084 1294 1979 475
Size: 609 210 685 2743
Fragments arranged by size:
2743 685 609 210
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4247
PvuII CAG'CTG
Cuts at: 476 1672 2032 476
Size: 1196 360 2691
Fragments arranged by size:
2691 1196 360
RsrII CG'GwC_CG
Cuts at: 2442 2442
Size: 4247
SacI G_AGCT'C
Cuts at: 1261 1261
Size: 4247
SacII CC_GC'GG
Cuts at: 503 503
Size: 4247
SalI G'TCGA_C
Cuts at: 1284 1284
Size: 4247
SapI GCTCTTCn'nnn_
Cuts at: 2270 2480 2270
Size: 210 4037
SbfI CC_TGCA'GG
Cuts at: 1294 1294
Size: 4247
ScaI AGT'ACT
Cuts at: 3764 3764
Size: 4247
SfcI C'TryA_G
Cuts at: 33 471 1080 1290 1975 2943 3134 33
Size: 438 609 210 685 968 191 1146
Fragments arranged by size:
1146 968 685 609 438 210 191
SfoI GGC'GCC
Cuts at: 1679 1927 1679
Size: 248 3999
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4247
SmaI CCC'GGG
Cuts at: 491 931 1269 491
Size: 440 338 3469
Fragments arranged by size:
3469 440 338
SmlI C'TyrA_G
Cuts at: 465 951 1402 2317 3031 3293 465
Size: 486 451 915 714 262 1419
Fragments arranged by size:
1419 915 714 486 451 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4247
SphI G_CATG'C
Cuts at: 1300 1800 1300
Size: 500 3747
StyI C'CwwG_G
Cuts at: 107 415 1381 1735 107
Size: 308 966 354 2619
Fragments arranged by size:
2619 966 354 308
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 265 753 1830 1963 2422 2631 265
Size: 488 1077 133 459 209 1881
Fragments arranged by size:
1881 1077 488 459 209 133
TatI w'GTAC_w
Cuts at: 521 915 1221 3762 521
Size: 394 306 2541 1006
Fragments arranged by size:
2541 1006 394 306
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3764 3796 3764
Size: 32 4215
Tth111I GACn'n_nGTC
Cuts at: 2044 2044
Size: 4247
XbaI T'CTAG_A
Cuts at: 1278 1278
Size: 4247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 380 1742 380
Size: 1362 2885
XmaI C'CCGG_G
Cuts at: 489 929 1267 489
Size: 440 338 3469
Fragments arranged by size:
3469 440 338
ZraI GAC'GTC
Cuts at: 507 1025 507
Size: 518 3729
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmtI BsiWI BsmBI
BspEI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
DraIII FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PshAI PsiI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI